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Journal of Forestry Research 2021年第1期32卷 249-262页

作者： Wei tang anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA 2 Stadium Drive University of North Carolina at Chapel HillChapel HillNC 27514USA

Transcription factors can be used to engineer plants for enhanced ***,the mechanism(s)by which the C2H2-type zinc fi nger transcription factor enhances pathogen resistance in cells is not fully ***,Agrobacterium tumefaciens carrying the gene for Arabidopsis thaliana cysteine2/histidine2-type transcription factor 6(ZAT6)was used to engineer rice(Oryza sativa L.),cotton(Gossypium hirsutum L.),and slash pine(Pinus elliottii Engelm.)to generate transgenic cell *** cells were then inoculated with the pathogenic bacterium Pseudomonas *** to the control,cell viability of transgenic cells increased 39–47%and growth rate increased 9–15%by 7 days after inoculation in rice,cotton and slash *** phosphatase activity and alkaline phosphatase activity and transcript levels of Ca 2+-dependent protein kinase genes OsCPK1,OsCPK2,OsCPK6,and OsCPK8 and mitogen-activated protein kinase genes OsMAPK1,OsMAPK2,OsMAPK3,and OsMAPK8 increased signifi cantly in transgenic rice cells by 3 day after inoculation,and extracellular pH had decreased by 10–14%by 96 min after inoculation in transgenic rice,cotton and slash pine *** results suggest that ZAT6 enhances *** resistance in plant cells by modulating transcription of CPK and MAPK and oxidase activity.

关键词： Ca^2+-dependent protein kinase Mitogenactivated protein kinase Oxidase Pathogen Pinus

Overexpression of Arabidopsis thaliana malonyl-CoA synthetase gene enhances cold stress tolerance by activating mitogen-activated protein kinases in plant cells

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Journal of Forestry Research 2021年第2期32卷 741-753页

作者： Wei tang anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA 101 Stadium Drive University of North Carolina at Chapel HillChapel HillNC 27514USA

Malonyl-CoA synthetases may modulate cell responses to abiotic stress by regulating stress-related signaling transduction pathways or activating expression of transcription ***,the molecular mechanism of cold stress tolerance enhanced by malonyl-CoA synthetase is not fully ***,we report that overexpression of the Arabidopsis thaliana malonyl-CoA synthetase gene AAE13.1 resulted in increased cell viability and growth rate and decreased thiobarbituric acid reactive substances under cold stress in rice(Oryza sativa L.),tobacco(Nicotiana tabacum),and slash pine(Pinus elliottii Engelm.).AAE13.1 was associated with cold stress tolerance by increasing the activity of ascorbate peroxidase,catalase,polyphenol oxidase,and peroxidase and the accumulation of acid phosphatase and alkaline *** six rice mitogenactivated protein kinase(MAPK)genes examined,AAE13.1 overexpression increased the expression of OsMAPK genes during cold ***13.1 activated expression of stressresponse genes OsMAPK1,OsMAPK2,and OsMAPK3,indicating that AAE13.1 enhances cold stress tolerance by regulating expression of MAPK genes in plant *** results increase our understanding of cold stress tolerance in species of monocotyledons,dicotyledons,and gymnosperms.

关键词： Cold stress Malonyl-CoA synthetase gene MAPK transcription factor Phosphatase Pinus

Transcriptional mechanisms regulating gene expression and determining cell fates in plant development

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Journal of Forestry Research 2017年第5期28卷 863-880页

作者： Wei tang anna y.tang College of Arts and Science East Carolina University University of North Carolina at Chapel Hill

Transcriptional regulatory mechanisms that control transcriptional regulators, target genes, and their interactions provide new insights into general development processes throughout the life cycle of the plant. Although different molecular mechanisms that regulate plant growth and development have been identified, detailed transcriptional mechanisms that control gene expression, modulate developmental programmes, and determine cell fates in plant development are not fully understood. To increase our understanding on transcriptional mechanisms regulating diverse processes in plant development, we have reviewed the regulation of transcription during the process of development including transcriptional mechanisms regulating root, stem, leaf, flower, seed, embryo, endosperm, ovule, fruit, and chloroplast development. We have summarized the interaction, expression, transport, signaling events of transcriptional regulators and their targets in a number of model plants and highlighted the involvement of hormones and microRNAs in plant development. Understanding the precise transcriptional mechanisms regulating gene expression in plant development will be valuable for plant molecular breeding.

关键词： MicroRNAs Molecular breeding Plant development Root formation Transcriptional regulators

Applications and roles of the CRISPR system in genome editing of plants

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Journal of Forestry Research 2017年第1期28卷 15-28页

作者： Wei tang anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA University of North Carolina at Chapel Hill 101 Stadium DriveChapel HillNC 27514USA

Genome editing is a valuable tool to target specific DNA sequences for mutagenesis in the genomes of microbes, plants, and animals. Although different genome editing technologies are available, the clustered regularly interspaced short palindromic repeats/Cas9 （CRISPR/ Cas9） system, which utilizes engineered endonucleases to generate a double-stranded DNA break （DSB） in the target DNA region and subsequently stimulates site-specific mutagenesis through DNA repair machineries, is emerging as a powerful genome editing tool for elucidating mecha- nisms of protection from plant viruses, plant disease resistance, and gene functions in basic and applied research. In this review, we provide an overview of recent advances in the CRISPR system associated genome editing in plants by focusing on application of this technology in model plants, crop plants, fruit plants, woody plants and grasses and discuss how genome editing associated with the CRISPR system can provide insights into genome modifications and functional genomics in plants.

关键词： CRISPR system break Functional genomics modifications Double-stranded DNA Genome editing Genome

MicroRNAs associated with molecular mechanisms for plant root formation and growth

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Journal of Forestry Research 2016年第1期27卷 1-12页

作者： Wei tang anna y.tang Department of Biology East Carolina University 101 Stadium Drive University of North Carolina at Chapel Hill

Although roots are so important for plant growth and crop productivity, the molecular mecha- nism（s） of root formation and growth is not fully under- stood. To increase our understanding of the underlying molecular mechanisms, here we review microRNA regu- lation of processes related to root formation： the regulation of transcription factors, nutrient uptake, stress signaling, and growth signaling. We have summarized the interaction, expression, transport, and signaling events that involve microRNAs in ideal and stressed conditions in a number of model plants, highlighting the involvement of microRNAs in root formation. MicroRNAs are now known to be important players in root initiation, development, and growth; understanding the precise mechanisms involved will be valuable for plant molecular breeding to develop high-yielding crops with high stress resistance and low nutrient requirements.

关键词： MicroRNAs, Nutrient. Root formationStress response Transcription factor

Transgenic woody plants for biofuel

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Journal of Forestry Research 2014年第2期25卷 225-236页

作者： Wei tang anna y.tang Department of Biology East Carolina University 101 Stadium Drive University of North Carolina at Chapel Hill

Transgenic trees as a new source for biofuel have brought a great interest in tree biotechnology. Genetically modifying forest trees for ethanol production have advantages in technical challenges, costs, environmental concerns, and financial problems over some of crops. Genetic engineering of forest trees can be used to reduce the level of lignin, to produce the fast-growing trees, to develop trees with higher cellulose, and to allow the trees to be grown more widely. Trees can establish themselves in the field with less care of farmers, compared to most of crops. Transgenic crops as a new source for biofuel have been recently reviewed in several reviews. Here, we overview transgenic woody plants as a new source for biofuel including genetically modified woody plants and environment; main focus of woody plants genetic modifications; solar to chemical energy transfer; cellulose biosynthesis; lignin biosynthesis; and cellulosic ethanol as biofuel.

关键词： biofuel cell wall cellulose lignin transgenic tree

Biological significance of RNA-seq and single-cell genomic research in woody plants

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Journal of Forestry Research 2019年第5期30卷 1555-1568页

作者： Wei tang anna y.tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA University of North Carolina at Chapel Hill 101 Stadium DriveChapel HillNC 27514USA

RNA-seq and single-cell genomic research emerge as an important research area in the recent years due to its ability to examine genetic information of any number of single cells in all living *** knowledge gained from RNA-seq and single-cell genomic research will have a great impact in many aspects of plant *** this review,we summary and discuss the biological significance of RNA-seq and single-cell genomic research in plants including the single-cell DNA-sequencing,RNA-seq and single-cell RNA sequencing in woody plants,methods of RNA-seq and single-cell RNA-sequencing,single-cell RNA-sequencing for studying plant development,and single-cell RNA-sequencing for elucidating cell type *** will focus on RNA-seq and single-cell RNA sequencing in woody plants,understanding of plant development through single-cell RNAsequencing,and elucidation of cell type composition via single-cell *** presented in this review will be helpful to increase our understanding of plant genomic research in a way with the power of plant single-cell RNA-sequencing analysis.

关键词： Cell type composition Genomics Plant development Single-cell RNA-sequencing methods

Advancing the National Immunization Program in an era of achieving universal vaccine coverage in China and beyond

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贫困所致传染病(英文) 2024年第2期13卷 1-5页

作者： Chen Shu Rodewald Lance E. Heng Du anna tang Shenglan ARC Centre of Excellence in Population Ageing Research (CEPAR) University of New South Wales Sydney Australia School of Risk and Actuarial Studies University of New South Wales Sydney Australia Department of the National Immunization Program Chinese Center for Disease Control and Prevention National Immunization Program China CDC Beijing China China Country Office Bill &amp Melinda Gates Foundation Beijing China Duke Global Health Institute Duke University Durham NC USA Global Health Research Center Duke Kunshan University No. Duke Avenue Kunshan Jiangsu China SingHealth Duke-NUS Global Health Institute Duke-NUS Singapore Singapore

Background Immunization is a cornerstone of public health. Despite great success, China’s National Immunization Program (NIP) faces challenges, such as the integration of several World Health Organization-recommended vaccines and other systemic issues. The Innovation Laboratory for Vaccine Delivery Research (VaxLab), supported by the Bill & Melinda Gates Foundation and established in 2021 at Duke Kunshan University, focuses on enhancing China’s NIP through research and policy advocacy. This editorial aims to summarize the key findings of the manuscripts published in the collection contributed by VaxLab team and set the future research *** findings The collection contains eleven manuscripts discussing China’s immunization landscape and strategies to improve coverage, particularly for non-NIP vaccines like human papillomavirus vaccine (HPV), pneumococcal conjugate vaccine (PCV),Haemophilus influenzae type b vaccine (Hib), and rotavirus vaccines. Key findings include: (i) The COVID-19 vaccination campaign demonstrated China’s capacity for rapid, large-scale immunization efforts, suggesting potential for broader vaccine coverage improvements; (ii) Efforts in combating cervical cancer through the HPV vaccine indicate progress but also highlight challenges like vaccine supply and equitable access; (iii) The lag in adopting higher-valent paediatric combination vaccines in China needs attention to address regulatory and health system hurdles; (iv) Disparities in access to non-NIP vaccines underscore the need for government initiatives to improve vaccine coverage, especially for remote areas and marginalized populations; (v) Original studies emphasize the influence of caregivers’ knowledge, health workers’ financial incentives, and concerns about vaccine efficacy on immunization rates; (vi) Case studies from the Weifang City of China and Indonesia to introduce PCV offer insights on successful vaccine introduction strategies and the impact of innovative financing and g

关键词： National Immunization Program New vaccine introduction Universal vaccine coverage Health system strengthening

丹参素对UVB诱导的皮肤光老化小鼠的保护作用和抗氧化机制研究

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日用化学工业（中英文） 2024年第1期54卷 65-72页

作者：王安娜方梦婕唐超岳天翔江西省医药学校江西南昌330020 江西中医药大学江西南昌330004

研究丹参素(DSS)对紫外线B(UVB)诱导的皮肤光老化(SP)小鼠的保护作用和抗氧化机制。建立了UVB诱导的SP小鼠模型,使用3个剂量的DSS(20,40和80 mg/(kg·d))治疗小鼠8周,结束后分别测定了各组小鼠的表皮含水量。通过HE染色评价皮肤组织形态... 详细信息

研究丹参素(DSS)对紫外线B(UVB)诱导的皮肤光老化(SP)小鼠的保护作用和抗氧化机制。建立了UVB诱导的SP小鼠模型,使用3个剂量的DSS(20,40和80 mg/(kg·d))治疗小鼠8周,结束后分别测定了各组小鼠的表皮含水量。通过HE染色评价皮肤组织形态,Masson三色染色评价胶原蛋白沉积。按照试剂盒说明测定皮肤组织中氧化应激指标(SOD、CAT、GSH-Px和MDA)和炎症指标(TNF-α和IL-6)水平。通过RT-qPCT和Western blotting检测了皮肤组织中MMP-1、Collagen I、Nrf2、Keap1、HO-1、NF-κB p65和p-NF-κB p65的mRNA或蛋白水平。结果显示,DSS剂量依赖性地提高了UVB诱导的SP小鼠表皮含水量,减轻皮肤损伤,促进胶原形成(P<0.05)。DSS抑制了UVB诱导的SP小鼠皮肤组织中MMP-1的转录和表达,促进了Collagen的转录和表达(P<0.05)。DSS升高了UVB诱导的SP小鼠皮肤组织中SOD、CAT和GSH-Px的水平,降低了MDA的水平(P<0.05)。DSS降低了UVB诱导的SP小鼠皮肤组织中TNF-α和IL-6的水平(P<0.05)。DSS促进了UVB诱导的SP小鼠皮肤组织中Nrf2和HO-1的转录和表达,抑制了Keap1的转录和表达(P<0.05)。DSS抑制了UVB诱导的SP小鼠皮肤组织中p-NF-κB p65的表达(P<0.05)。本研究表明DSS可有效改善UVB诱导的小鼠SP,其机制与Nrf2和NF-κB信号通路有关。

关键词：丹参素紫外线B 皮肤光老化小鼠抗氧化

尖吻蝮蛇毒腺的缓激肽2型受体结合多肽的筛选与验证

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四川动物 2024年第4期43卷 361-372页

作者：蒋安娜刘杨刘桂娜唐业忠陆方四川大学华西医院成都610041 中国科学院成都生物研究所成都610041

蛇毒已被证明具有抗炎活性,其中包含许多免疫原性弱、活性强的短肽小分子。蛇毒腺是分泌毒液的器官,含有毒素成分及调控其分泌过程的大量生物活性成分。缓激肽2型受体(B2R)参与重要的细胞内信号通路,作为炎症的调节因子成为潜在的抗炎... 详细信息

蛇毒已被证明具有抗炎活性,其中包含许多免疫原性弱、活性强的短肽小分子。蛇毒腺是分泌毒液的器官,含有毒素成分及调控其分泌过程的大量生物活性成分。缓激肽2型受体(B2R)参与重要的细胞内信号通路,作为炎症的调节因子成为潜在的抗炎药物开发的靶点。为了获得特异性的B2R结合肽,利用尖吻蝮蛇Deinagkistro-don acutus毒腺T7噬菌体文库对靶点B2R进行3轮生物淘选,测序获得了多个具有潜在结合能力的序列。通过序列长度、溶解性预测、稳定性预测和分子对接等一系列生物信息学分析,最终确定1个含25个氨基酸的肽段,命名为DAvp-4。合成该肽段,通过表面等离子体共振技术验证了DAvp-4和B2R的结合能力,在脂多糖诱导的巨噬细胞模型及糖尿病视网膜病变小鼠模型中肯定了其抗炎作用。此研究不但获得了一个具有成药潜力的毒腺多肽,还显示了噬菌体展示技术在筛选天然产物成分研究中的有效性。

关键词：噬菌体展示毒腺表面等离子体共振缓激肽2型受体