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地氟烷和七氟烷预处理信号链中活性氧生成和线粒体解偶联的差异

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麻醉与镇痛 2010年第5期6卷 17-23页

作者： Filip Sedlic, MD Danijel Pravdic, MD Marko Ljubkovic, MD, PhD Jasna Marinovic, MD anna stadnicka, PhD Zeljko J. Bosnjak, PhD 胡乃琴(译) 杜金(校) Departments of AnesthesiologyMedical College of Wisconsin Milwaukee Wisconsin Departments of Physiology Medical College of Wisconsin Milwaukee Wisconsin 不详

背景麻醉药预处理的信号传导链已被广泛研究，但是许多问题仍然有待解决。本研究拟评价两种现代麻醉药地氟烷和七氰烷进行心肌细胞预处理过程中，活性氧（ROS）和线粒体解偶联的作用。方法酶解分离成年大鼠心室肌细胞，利用细胞存活实... 详细信息

背景麻醉药预处理的信号传导链已被广泛研究，但是许多问题仍然有待解决。本研究拟评价两种现代麻醉药地氟烷和七氰烷进行心肌细胞预处理过程中，活性氧（ROS）和线粒体解偶联的作用。方法酶解分离成年大鼠心室肌细胞，利用细胞存活实验评估地氟烷和七氟垸预处理保护心肌细胞免受氧化应激诱导的细胞死亡的效力。应用共聚焦显微镜实时监测ROS生成和黄素蛋白荧光。黄素蛋白荧光是黄素蛋白氧化和线粒体解偶联的标志。应用ROS清除剂Trolox通过细胞生存和共聚焦实验评估麻醉药导致ROS生成增加的作用。结果地氟烷或七氟烷参与的心肌细胞预处理明显减少氧化应激诱导的细胞死亡。这种效应与心肌细胞急性接触麻醉药过程中增加ROS生成和黄素蛋白氧化相一致。地氟烷诱导较七氟垸明显提高ROS生成和黄素蛋白氧化。ROS清除剂Trolox取消麻醉药的预处理效力，减弱黄素蛋白氧化。结论地氟烷和七氟烷预处理保护大鼠分离心肌细胞免受氧化应激诱导的细胞死亡。ROS清除剂废除两种麻醉药的预处理作用，减弱麻醉药诱导的线粒体解偶联，这表明ROS在麻醉药诱导预处理中起关键作用，提示ROS是线粒体解偶联的上游信号。地氟烷与七氟烷相比，刺激ROS生成和线粒体解偶联的作用更强。

关键词：信号传导链活性氧生成预处理地氟烷解偶联线粒体七氟烷保护心肌细胞

Venous and arterial thromboembolism in patients with inflammatory bowel diseases

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World Journal of Gastroenterology 2021年第40期27卷 6757-6774页

作者： Antoni Stadnicki Izabela stadnicka Department of Physiology Faculty of MedicineUniversity of TechnologyKatowice 41-209Poland Department of Molecular Medicine Medical University of SilesiaFaculty of PharmacySosnowiec 41-200Poland

The risk of thromboembolism(TE)is increased in patients with inflammatory bowel disease(IBD),mainly due to an increased risk of venous TE(VTE).The risk of arterial TE(ATE)is less pronounced,but an increased risk of cardiovascular diseases needs to be addressed in IBD *** predisposes to arterial and venous thrombosis through similar prothrombotic mechanisms,including triggering activation of coagulation,in part mediated by impairment of the intestinal barrier and released bacterial *** in IBD has clinical specificities,i.e.,an earlier first episode in life,high rates during both active and remission stages,higher recurrence rates,and poor *** increased likelihood of VTE in IBD patients may be related to surgery,the use of medications such as corticosteroids or tofacitinib,whereas infliximab is ***-term complications of VTE can include post-thrombotic syndrome and high recurrence rate during post-hospital discharge.A global clot lysis assay may be useful in identifying patients with IBD who are at risk for *** VTEs occur in IBD outpatients;therefore,outpatient prophylaxis in high-risk patients is *** is crucial to continue focusing on prevention and adequate treatment of VTE in patients with IBD.

关键词： Inflammatory bowel disease Thrombosis Coagulation Bacterial components Prophylaxis Post-thrombotic syndrome

The influence of in ovo injection with the prebiotic DiNovoon the development of histomorphological parameters of the duodenum, body mass and productivity in large-scale poultry production conditions

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Journal of Animal Science and Biotechnology 2017年第4期8卷 984-991页

作者： Adrianna Sobolewska Gabriela Elminowska-Wenda Joanna Bogucka Agata Dankowiakowska anna Kulakowska Agata Szczerba Katarzyna stadnicka Michal Szpinda Marek Bednarczyk Department of Animal Biochemistry and Biotechnology University of Science and Technology in Bydgoszcz Mazowiecka 28 Street 85-084 Bydgoszcz Poland Department of Normal Anatomy the Ludwik Rydygier Collegium Medicum in Bydgoszcz the Nicolaus Copernicus University in Torun 24 Karlowicza Street Bydgoszcz 85-092 Poland

Background: Among various feed additives currently used in poultry nutrition, an important role is played by bioactive substances, including prebiotics. The beneficial effect of these bioactive substances on the gastrointestinal tract and immune system give rise to improvements in broiler health and performance nutrition, thus increasing the productivity of these birds. An innovative method for introducing bioactive substances into chickens is the in ovo injection into eggs intended for hatching. The aim of the study was to evaluate the development of histomorphological parameters of the duodenum and productivity in chickens injected in ovo with the prebiotic DiNovo~?(extract of Laminaria species of seaweed, BioA tlantis Ltd., Ireland) on d 12 of incubation, under large-scale, high density poultry production ***: There was no significant impact of the injection of DiNovo~? prebiotic on the production parameters of broiler chickens(body weight, FCR, EBI and mortality) obtained on d 42 of rearing. No significant impact of the DiNovo~? injection on the duodenum weight and length was observed, as wel as on the CSA, diameter and muscular layer thickness of the duodenum. The in ovo injection of DiNovo~? significantly increased the width of the duodenal vil i(P < 0.05) and crypt depth(P < 0.01) of chickens on d 21 of rearing. Other histomorphological parameters of duodenal vil i at d 42 of chickens rearing such as: the height, width, and cross section area of vil i were significantly greater in chickens from the control group compared to those from the DiNovo~? group(P < 0.05 and P < 0.01).Conclusions: In conclusion, this study demonstrates that injection of DiNovo~? prebiotic into the air chamber of egg significantly influences the histomorphological parameters on d 21 of rearing without negatively affecting productivity in chickens at the end of rearing.

关键词： Chicken Duodenum Histomorphological parameters In ovo Prebiotic Productivity

The impact of synbiotic administration through in ovo technology on the microstructure of a broiler chicken small intestine tissue on the 1^(st) and 42^(nd) day of rearing

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Journal of Animal Science and Biotechnology 2018年第1期9卷 167-174页

作者： A.Sobolewska J.Bogucka A.Dankowiakowska G.Elminowska-Wenda K.stadnicka M.Bednarczyk Department of Animal Biochemistry and Biotechnolocjy University of Science and Technology in Bydgoszcz Mazowiecka 28 85-084 Bydgoszcz Poland Department of Normal Anatomy Ludwik Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Toruri Karlowicza 24 85-092 Bydgoszcz Poland

Background： Application the innovative method which is in ovo technology provides a means of modulating the immune system at early embryonic stages. The aim of study was to determine influence of the in ovo stimulation, on d12 of incubation, with synbiotics（synbiotic 1-L. salivarius IBB3154 ＋ Bi^2tos, Clasado Ltd. and the synbiotic 2-L. plantarum IBB3036 ＋ lupin RFOs） on the microstructure of duodenum, jejunum and ileum in the 1^（st） and 42^（nd）day of ***： On the 1^（st） day of chickens life, in the duodenum of both experimental groups（SYN1 and SYN2）, a significantly higher and wider intestinal vil i as wel as a significantly larger absorbent surface of these villi were found in comparison with the Control group（P ≤ 0.01）. On the 42^（nd）day of rearing the beneficial effect of synbiotic 1 was reflected by the numerical y higher vil i（no statistical differences） with a larger surface（P ≤ 0.01） in the duodenum in the SYN1 group compare to the Control group. In the jejunum on the 1^（st） day of life, in the SYN1 group, significantly higher vil i than in the Control group, with a simultaneous decrease in the depth of crypts（P ≤ 0.01）, and also the largest width of vil i and their absorbent area（P ≤ 0.01） in comparison to the other groups were found. On the 42^（nd）day of life, in the jejunum, an increase in the height of the vil i whilst reducing the crypt depth in the SYN2 group was found（P ≤ 0.01）. In turn, in the SYN1 group, there were significantly more neutral goblet cel s observed compared with the control group（P ≤ 0.05）. In the ileum of 1-day-old chickens, the widest vil i（P ≤ 0.05） and the deepest crypts（P ≤ 0.01） were found in the SYN2 group. In the same group, there was also the least amount of neutral goblet cel s in comparison to the other groups（P ≤ 0.05）.Conclusions： We observed that synbiotic 1 and 2 beneficial y affected the examined characteristics on the 1^（st） and 42^（nd） day of life. The obtained results al ow us to conclude that the use of

关键词： Broiler chicken In ovo Small intestine Synbiotics

GLP1 and GIP are involved in the action of synbiotics in broiler chickens

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Journal of Animal Science and Biotechnology 2018年第2期9卷 451-459页

作者： Pawel Antoni Kolodziejski Maciej Sassek Daniela Chalupka Natalia Leciejewska Leszek Nogowski Pawel Mackowiak Damian Jozefiak Katarzyna stadnicka Maria Siwek Marek Bednarczyk Tomasz Szwaczkowski Ewa Pruszynska-Oszmalek Department of Animal Physiology and Biochemistry Poznan University of Life Sciences Department of Animal Nutrition and Feed Management Poznan University of Life Sciences Department of Animal Biochemistry and Biotechnology UTP University of Science and Technology Department of Genetics and Animal Breeding Poznan University of Life Sciences

Background: In order to discover new strategies to replace antibiotics in the post-antibiotic era in meat-type chicken production, two new synbiotics were tested:(Lactobacillus salivarius IBB3154 plus galactooligosaccharide(Syn1) and Lactobacillus plantarum IBB3036 plus raffinose family oligosaccharides(Syn2).Methods: The synbiotics were administered via syringe, using a special automatic system, into the egg air chamber of Cobb 500 broiler chicks on the 12 thday of egg incubation(2 mg of prebiotics + 105 cfu bacteria per egg).Hatched roosters(total 2,400) were reared on an experimental farm, kept in pens(75 animals per pen), with free access to feed and water. After 42 d animals were slaughtered. Blood serum, pancreas, duodenum and duodenum content were ***: Syn2 increased trypsin activity by 2.5-fold in the pancreas and 1.5-fold in the duodenal content. In the duodenum content, Syn2 resulted in ca 30% elevation in lipase activity and 70% reduction in amylase ***1 and Syn2 strongly decreased expression of m RNA for GLP-1 and GIP in the duodenum and for GLP-1 receptors in the pancreas. Simultaneously, concentrations of the incretins significantly diminished in the blood serum(P < 0.05). The decreased expression of incretins coincides with changed activity of digestive enzymes in the pancreas and in the duodenal content. The results indicate that incretins are involved in the action of Syn1 and Syn2 or that they may even be their target. No changes were observed in key hormones regulating metabolism(insulin, glucagon, corticosterone, thyroid hormones, and leptin) or in metabolic indices(glucose,NEFA, triglycerides, cholesterol). Additionally, synbiotics did not cause significant changes in the activities of alanine and aspartate aminotransferases in broiler chickens. Simultaneously, the activity of alkaline phosphatase and gamma glutamyl transferase diminished after Syn2 and Syn1, ***: The selected synbiotics may be used as

关键词： GIP GLP-1 Incretins In ovo Synbiotics