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α-Synuclein arginylation in the human brain

Translational Neurodegeneration 2022年第1期11卷 654-666页

作者： Jun Zhao Buyan Pan marie Fina Yun Huang marie Shimogawa Kelvin C.Luk Elizabeth Rhoades EJames Petersson Dawei W.Dong anna Kashina Department of Biomedical Sciences University of Pennsylvania School of Veterinary MedicinePhiladelphiaPennsylvania 19104USA Department of Chemistry University of Pennsylvania School of Arts and SciencesPhiladelphiaPennsylvania 19104USA Center for Neurodegenerative Disease Research Department of Pathology and Laboratory MedicineUniversity of Pennsylvania Perelman School of MedicinePhiladelphiaPennsylvania 19104USA.

Background: Alpha-synuclein (α-syn) exhibits pathological misfolding in many human neurodegenerative disorders. We previously showed that α-syn is arginylated in the mouse brain and that lack of arginylation leads to neurodegen-eration in mice. methods: Here, we tested α-syn arginylation in human brain pathology using newly derived antibodies in combina-tion with Western blotting, biochemical assays, and experiments in live neurons. Results: We found that α-syn was arginylated in the human brain on E46 and E83, two sites previously implicated in α-syn pathology and familial cases of Parkinson’s disease. The levels of arginylation in different brain samples ranged between ~ 3% and ~ 50% of the total α-syn pool, and this arginylation nearly exclusively concentrated in the subcellu-lar α-syn fraction that sedimented at low centrifugation speeds and appeared to be simultaneously targeted by multi-ple posttranslational modifications. Arginylated α-syn was less susceptible to S129 phosphorylation and pathological aggregation in neurons. The arginylation level inversely correlated with the overall α-syn levels and with patient age, suggesting a possible causal relationship between arginylation decline and α-syn-dependent neuropathology. Conclusion: We propose that α-syn arginylation constitutes a potential neuroprotective mechanism that prevents its abnormal accumulation during neurodegeneration and aging in the human brain.

关键词： Arginylation Neurodegeneration Aging α-Synuclein

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Nest-site selection by the white-tailed eagle and black stork–implications for conservation practice

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Forest Ecosystems 2020年第4期7卷 781-792页

作者： Grzegorz Zawadzki Dorota Zawadzka anna Sołtys Stanisław Drozdowski Department of Forest Protection Institute of Forest SciencesWarsaw University of Life SciencesNowoursynowska 15902-776 WarszawaPoland. Department of Forestry University ofŁódźBranch in Tomaszów MazowieckiKonstytucji maja 65/67 97-200 Tomaszów MazowieckiPoland.3Department of SilvicultureInstitute of Forest SciencesWarsaw University of Life SciencesNowoursynowska 15902-776 WarszawaPoland.

Background:Nesting trees and habitat represent the key factor underpinning stand selection by forest-dwelling *** two large European species–the black stork(Ciconia nigra L.)and white-tailed eagle(Haliaaetus albicilla L.)–are known to require old,large trees for nesting,we sought to investigate further by comparing species requirements at the levels of the nesting tree,nesting stand,and *** entailed a detailed examination of forest features within circles of radius 15 m surrounding 16 and 19 trees holding the nests of storks and eagles *** same parameters were also checked in the vicinity of 50 randomly-selected mature ***:Our results indicate different nesting preferences,with the eagles entirely confining themselves to Scots pine(Pinus sylvestris L.)–a species also chosen by black storks,which nevertheless regularly favour pedunculate oak(Quercus robur L.).Both species select trees of greater girth than the random ones,but white-tailed eagles choose to nest in the vicinity of patches of mature old stands with a loose canopy,to the extent that nesting trees and surrounding trees are of similar *** contrast,black storks prefer“veteran”trees with low-set crowns that are much older than any others in their *** trees of the eagles are away from roads and close to lakes,while black storks do not avoid ***:As the ages of nesting trees of both species are greater than ages at final cutting in this region’s managed forest,silvicultural measures will need to be adjusted,with small patches of forest spared,or larger areas as“islands”of ***,as tending and thinning are engaged in,certain trees with horizontal branches will need to remain,with relict trees also left *** it happens,all of these recommendations are anyway key elements of close-to nature silviculture and multifunctional forestry.

关键词： Augustów Forest Poland Ciconia nigra Haliaeetus albicilla Nesting requirements Protection of nest sites Old trees Forest management

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Diet composition of the lizard P odarcis lilfordi (Lacertidae) on 2 small islands: an individualresource network approach

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Current Zoology 2020年第1期66卷 39-49页

作者： Silvia SANTAmARIA Camilla Aviaaja ENOKSEN Jens m.OLESEN Giacomo TAVECCHIA Andreu ROTGER Jose manuel IGUAL anna TRAVESET Global Change Research Group Inst.Mediterrani d'Estudis Avanqats(CSIC-UIB)C/Miquel Marques 21E07190 EsporlesMallorcaBalearic IslandsSpain Section for Ecoinformatics&Biodiversity Department of BioscienceAarhus UniversityAarhus C8000Denmark Animal Ecology and Demography Group Inst.Mediterrani d'Estudis Avanqats(CSIC-UIB)C/Miquel Marques 21EsporlesMallorcaBalearic IslandsE07190Spain

Despite it is widely accepted that intrapopulation variation is fundamental to ecological and evolutionary processes,this level of information has only recently been included into network analysis of species/population *** done,it has revealed non-random patterns in the distribution of trophic *** in resource use among individuals is the most recurrent observed pattern,often accompanied by an absence of modularity,but no previous studies examine bipartite *** use network analysis to describe the diet composition of the Balearic endemic lizard Podarcis lilfordi in 2 islets at population and individual levels,based on the occurrence of food items in fecal *** objectives are to 1)compare niche structure at both levels,2)characterize niche partition using nestedness and modularity,and 3)assess how size,sex,season,and spatial location influence niche *** population-level niche width was wide,but narrow at the level of the *** islet networks were nested,indicating similar ranking of the food preferences among individuals,but also modular,which was partially explained by *** and body size did not notably affect diet *** niche overlap and therefore possibly relaxed competition were observed among females in one of the islets and during spring on both ***,higher modularity in autumn suggests that higher competition could lead to specialization in both populations,because resources are usually scarce in this *** absence of spatial location influence on niche might respond to fine-grained spatio-temporally distribution of food *** traits,not included in this study,could also influence resource partitioning.

关键词： Balearic Islands individual diet composition individual-level network modularity nestedness population niche width

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Halogen-specific total organic halogen analysis： Assessment by recovery of total bromine

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Journal of Environmental Sciences 2017年第8期29卷 340-348页

作者： markus Langsa Sebastien Allard Ina Kristiana anna Heitz Cynthia A.Joll Curtin Water Quality Research Centre Department of Chemistry Curtin University Perth Western Australia 6102 Australia Department of Civil Engineering Curtin University Perth Western Australia 6102 Australia Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan AlamUniversitas PapuaManokwaripapua Barat 98314Indonesia

Determination of halogen-specific total organic halogen（TOX） is vital for studies of disinfection of waters containing bromide, since total organic bromine（TOBr） is likely to be more problematic than total organic chlorine. Here, we present further halogen-specific TOX method optimisation and validation, focusing on measurement of TOBr. The optimised halogen-specific TOX method was validated based on the recovery of model compounds covering different classes of disinfection by-products（haloacetic acids, haloacetonitriles,halophenols and halogenated benzenes） and the recovery of total bromine（mass balance of TOBr and bromide concentrations） during disinfection of waters containing dissolved organic matter and bromide. The validation of a halogen-specific TOX method based on the mass balance of total bromine has not previously been reported. Very good recoveries of organic halogen from all model compounds were obtained, indicating high or complete conversion of all organic halogen in the model compound solution through to halide in the absorber solution for ion chromatography analysis. The method was also successfully applied to monitor conversion of bromide to TOBr in a groundwater treatment plant. An excellent recovery（101%）of total bromine was observed from the raw water to the post-chlorination stage. Excellent recoveries of total bromine（92%–95%） were also obtained from chlorination of a synthetic water containing dissolved organic matter and bromide, demonstrating the validity of the halogen-specific TOX method for TOBr measurement. The halogen-specific TOX method is an important tool to monitor and better understand the formation of halogenated organic compounds, in particular brominated organic compounds, in drinking water systems.

关键词： Total organic bromine Dissolved organic matter Bromide Chlorination Drinking water Health effects

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Identification of victims of the collapse of a mine tailing dam in Brumadinho

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Forensic Sciences Research 2022年第4期7卷 580-589页

作者： Ricardo moreira Araújo Yara Vieira Lemos Erlon Dias do Nascimento anna Helena Silva Paraizo Alberto Julius Alves Wainstein Ana Paula Drummond-Lage Faculty of medical Sciences of minas Gerais Belo HorizonteBrazil Instituto médico Legal AndréRoquette Belo HorizonteBrazil Corpo de Bombeiros militar de minas Gerais Juiz de ForaBrazil

The collapse of the B1 Dam of VALESAmining company in Brumadinho,minas Gerais,Brazil was the largest humanitarian disaster and occupational accident in the country’s history,and it posed challenges regarding the management and identification of multiple *** evaluated the impact of the iron ore tailings on the victims’*** examined the scientific identification of the victims and the dynamics of the disaster over the 1st year after it *** also determined the socio-demographic profiles of the *** this retrospective,cross-sectional study,we investigated the expert reports of the victims’biological remains from 25 January 2019 to 25 January *** analysed the socio-demographic data,identification methods,identification status,identification time,and necroscopic *** the study period,259 of 270 victims were identified,and 603 biological materials were analysed;among them,86.2%were body parts and 13.8%were whole *** the total cases registered that year,476(78.9%)were submitted during the first 10 weeks after the *** ridge analysis accounted for 67.9%of primary identifications and DNAanalysis did so for 91.6%of re-identification *** dismemberment was 3.4 times greater among mine workers than among community *** males accounted for the greatest number of victims(Pmatic injury was the prevalent single cause of *** examination revealed the occurrence of asphyxia in 7%of *** higher number of fatalities and greater dismemberment among employees than with community residents underlines the occupational dangers in the mining industry and clarifies the dynamics of the *** the initial weeks after the dam collapsed,friction ridge analysis was the most appropriate method for ***,DNAanalysis became the most-used technique for identification and re-identification owing to the great volume of body parts and decomposed biological ***

关键词： Disasters victim identification tailings mining legal medicine developing countries man-made disasters traumatic amputation

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电子转移途径中芳香族氨基酸的引入提高了细胞色素P450s的催化性能

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Chinese Journal of Catalysis 2023年第6期49卷 81-90页

作者：孟帅奇李忠玉季宇 anna Joelle Ruff 刘珞 mehdi D.Davari Ulrich Schwaneberg 亚琛工业大学生物技术研究所德国北京化工大学北京生物过程重点实验室北京100029 莱布尼茨植物生物化学研究所生物有机化学系德国莱布尼茨互动材料研究所德国

细胞色素P450s是用于生物合成的多功能催化剂.在P450催化循环中,需要两个电子来还原血红素铁,并通过电子转移途径(eTPs)激活随后的还原,该步骤是反应的限速步骤.本文重新设计了巨大芽孢杆菌P450 Bm3的e TPs,大幅提高了其催化性能.通过在... 详细信息

细胞色素P450s是用于生物合成的多功能催化剂.在P450催化循环中,需要两个电子来还原血红素铁,并通过电子转移途径(eTPs)激活随后的还原,该步骤是反应的限速步骤.本文重新设计了巨大芽孢杆菌P450 Bm3的e TPs,大幅提高了其催化性能.通过在P450 Bm3的e TP中引入芳香族氨基酸,“最佳”变体P2H02(A399Y/Q403F)的催化效率比P450 Bm3野生型催化效率提高了12.9倍(kcat/Km从65.8 L mol^(-1)s^(-1)提高到913.5 L mol^(-1)s^(-1)).分子动力学模拟和电子传递分析表明,在辅因子FmN和血红素之间引入的芳香族氨基酸可以显著提高电子转移速率和酶催化性能.同时,在电子传递途径中引入酪氨酸可以保护P450的催化中心,使其避免被氧化性中间产物所破坏,从而提高其催化效率.此外,引入芳香族氨基酸的策略被证明对其他P450(如CYP116B3)同样有效,改造后的酶表现出显著提高的催化效率.综上,本文策略有望拓展到其他带有长程电子传递链的氧化还原酶上,成为改造的通用策略.

关键词：定向进化 P450 Bm3 蛋白质工程电子传递理性设计

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BEA沸石后合成金属(Sn,Zr,Hf)改性:结合Lewis和Bronsted酸性进行级联催化

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Chinese Journal of Catalysis 2023年第12期55卷 200-215页

作者： Peerapol Pornsetmetakul Ferdy J.A.G.Coumans Rim C.J.van de Poll anna Liutkova Duangkamon Suttipat Brahim mezari Chularat Wattanakit Emiel J.m.Hensen 泰国科学与技术研究院能源科学与工程学院泰国埃因霍温理工大学化学工程系无机材料与催化实验荷兰

Sn,Zr和Hf等Lewis酸性金属中心改性的沸石是许多生物炼制相关反应(如碳水化合物异构化和呋喃meerwein-Pondorf-Verley(mPV)还原)中有应用前景的催化剂.在大孔BEA沸石框架中固载上述金属离子得到的催化剂性能良好.然而,制备该类催化剂通... 详细信息

Sn,Zr和Hf等Lewis酸性金属中心改性的沸石是许多生物炼制相关反应(如碳水化合物异构化和呋喃meerwein-Pondorf-Verley(mPV)还原)中有应用前景的催化剂.在大孔BEA沸石框架中固载上述金属离子得到的催化剂性能良好.然而,制备该类催化剂通常需采用昂贵的有机金属前体,并且需要经历多步制备过程.本文开发了一种简易的固态研磨法,采用简单的无机金属前体,在脱铝BEA沸石中成功掺入了高度分散的、高金属含量(Si/m比为50-75)的孤立Sn,Zr和Hf.在样品焙烧前,采用甲醇处理去除过量的金属,可使孤立金属位点高含量、有效地掺杂在BEA框架中.在改性的BEA沸石中,Lewis酸位点来自于开放的Sn,Zr和Hf孤立金属离子位点;Sn改性的BEA上Lewis酸位点还包括封闭的Sn位点,但开放的Sn位点表现出最高的Lewis酸性.Br?nsted酸性来自于受开放金属位点Lewis酸性金属离子摄动的硅羟基以及与开放金属位点相连的羟基.Sn,Zr,Hf改性的BEA沸石在肉桂醛级联还原醚化反应中表现出较好的催化活性.以异丙醇为溶剂,肉桂醛先在Lewis酸位点上发生mPV还原生成肉桂醇;随后,肉桂醇在Br?nsted酸位点发生醚化反应生成肉桂基丙基醚.其中,Sn改性的BEA沸石表现出最高的催化活性,这是因为其具有最强的Lewis酸性,这对第一步mPV反应至关重要.此外,将本研究中Sn改性优化固态离子交换法用于对不同形貌BEA沸石进行改性以提高其催化性能,结果表明,BEA沸石多级孔结构进一步有利于级联还原醚化反应.

关键词：分子筛 BEA沸石嫁接金属形态酸性

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Real-world performance analysis of a novel computational method in the precision oncology of pediatric tumors

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World Journal of Pediatrics 2023年第10期19卷 992-1008页

作者： Barbara Vodicska Júlia Déri Dóra Tihanyi Edit Várkondi EnikőKispéter Róbert Dóczi Dóra Lakatos anna Dirner mátyás Vidermann Péter Filotás Réka Szalkai-Dénes István Szegedi Katalin Bartyik Krisztina míta Gábor Réka Simon Péter Hauser György Péter Csongor Kiss miklós Garami István Peták Oncompass medicine Hungary Kft Retek Str.34Budapest 1024Hungary Division of Pediatric Hematology-Oncology Department of PediatricsFaculty of MedicineUniversity of DebrecenDebrecenHungary Department of Pediatrics Albert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Onco-Hematology Department Velkey LászlóPaediatric Health CentreMiskolcHungary Department of Paediatrics Semmelweis UniversityBudapestHungary Onco-Hematology Department Heim Pál Children’s HospitalBudapestHungary Department of Pharmacology and Pharmacotherapy Semmelweis UniversityBudapestHungary Department of Pharmaceutical Sciences University of Illinois at ChicagoChicagoUSA Genomate Health CambridgeMAUSA

Background The utility of routine extensive molecular profiling of pediatric tumors is a matter of debate due to the high number of genetic alterations of unknown significance or low evidence and the lack of standardized and personalized decision support *** drug assignment(DDA)is a novel computational method to prioritize treatment options by aggregating numerous evidence-based associations between multiple drivers,targets,and targeted *** has been validated to improve personalized treatment decisions based on the outcome data of adult patients treated in the SHIVA01 clinical *** aim of this study was to evaluate the utility of DDA in pediatric *** Between 2017 and 2020,103 high-risk pediatric cancer patients(m,and samples from 100 patients were eligible for further *** or blood samples were analyzed by whole-exome(WES)or targeted panel sequencing and other molecular diagnostic modalities and processed by a software system using the DDA algorithm for therapeutic decision ***,a molecular tumor board(mTB)evaluated the results to provide therapy *** Of the 100 cases with comprehensive molecular diagnostic data,88 yielded WES and 12 panel sequencing *** identified matching off-label targeted treatment options(actionability)in 72/100 cases(72%),while 57/100(57%)showed potential drug *** reached 88%(29/33)by 2020 due to the continuous updates of the evidence *** approved the clinical use of a DDA-top-listed treatment in 56 of 72 actionable cases(78%).The approved therapies had significantly higher aggregated evidence levels(AELs)than dismissed *** of WES results for targeted panels missed important mutations affecting therapy *** DDA is a promising approach to overcome challenges associated with the interpretation of extensive molecular profiling in the routine car

关键词： Computational decision support Pediatric tumors Precision oncology Tumor board

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金属污染和海洋酸化对海洋生物生理的影响

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Current Zoology 2015年第4期61卷 653-668页

作者： anna V. Ivanina Inna m. Sokolova Department of Biological Sciences University of North Carolina at Charlotte Charlotte NC 28223 USA

在全球环境中的变化，如海洋酸化（办公自动化）可能会影响人类的污染物，包括微量金属，威胁到海洋生态系统的完整性。我们分析最近的研究，对海洋生物的相互作用的影响，对海洋生物的相互作用，这些相互作用的生理基础。我们的分析表... 详细信息

在全球环境中的变化，如海洋酸化（办公自动化）可能会影响人类的污染物，包括微量金属，威胁到海洋生态系统的完整性。我们分析最近的研究，对海洋生物的相互作用的影响，对海洋生物的相互作用，这些相互作用的生理基础。我们的分析表明，CO2和金属高温反应强烈依赖于物种，发育阶段，金属生物化学和环境的高碳酸血症的程度，并不能直接从CO2引起的金属溶解性和形态变化预测。通过OA和微量金属暴露影响的关键生理功能涉及酸碱调节，亲蛋白周转和线粒体能量，反映其细胞和分子途径的CO2和金属的敏感性。生理作用下二氧化碳和金属之间的相互作用可能会影响生物体的能力，以保持交流-身份证的基本平衡，并减少能量可用于健身相关的功能，如生长，发展和重新生产，从而影响生存和河口种群的性能。也可以通过改变环境的高碳酸血症的捕食作用和食物链的金属传输影响海洋食物网。然而，我们在这一程度上，这些影响可以影响海洋生态系统的功能和完整性是有限的，由于疤痕-城市发表的研究和对某些分类群的偏见。今后的研究重点应包括金属X pCO2的相互作用的研究聚焦于关键的生理功能（包括酸碱，蛋白质和能量平衡）在更大范围的生态和经济上重要的海洋物种，以及包括田间种群自然前合影（和潜在的改编）在不同层次的环境[当前生态61金属和CO2（4）：653-668，2015 ]。

关键词：金属污染海洋生物生理基础酸化海洋生态系统相互作用 pCO2 全球环境

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银纳米颗粒的绿色合成:藻类生命周期对银纳米颗粒合成和长期稳定性的影响（英文）

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Transactions of Nonferrous metals Society of China 2018年第5期28卷 974-979页

作者： Oksana VELGOSOVA anna mRAZIKOVA Elena CIZmAROVA Jaroslav mALEK Institute of materials and Quality Engineering Faculty of Materials Metallurgy and Recycling Technical University of Kosice Letnfi 9/A 040 01 Kosice Slovakia Republic Department of materials Engineering Faculty of Mechanical EngineeringCzech Technical University in Prague Karlovo nam. 13 121 32 Prague 2 Czech Republic

用分别培养了1、2、3和4周的凯氏拟小球藻的四种不同的提取物,生物合成球形银纳米颗粒(AgNPs)。研究藻类生命周期对AgNPs合成的影响和不同的储存条件对AgNPs长期稳定性的影响。结果发现,藻类的年龄会影响AgNPs的合成率和溶液中的数量,... 详细信息

用分别培养了1、2、3和4周的凯氏拟小球藻的四种不同的提取物,生物合成球形银纳米颗粒(AgNPs)。研究藻类生命周期对AgNPs合成的影响和不同的储存条件对AgNPs长期稳定性的影响。结果发现,藻类的年龄会影响AgNPs的合成率和溶液中的数量,但不影响AgNPs的长期稳定性。UV–vis和TEm的结果显示,AgNPs的长期稳定性受储存温度的影响,低温更有利于其长期稳定性。储存在黑暗环境和5°C左右时,AgNPs表现出最佳的长期稳定性,而与藻类年龄无关,存储6个月以后,这些AgNPs仍然保持着球形,尺寸细小(5~20nm),且无团聚。

关键词：银纳米颗粒稳定性凯氏拟小球藻

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