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Stress Response Monitoring of Photoautotrophic.Higher Plant Suspension c.ltures by Fluoresc.nc. Imaging for High-Throughput Toxic.c.mpound Sc.eening

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Journal of Environmental Protec.ion 2017年第6期8卷 678-692页

作者： anna Segec.va Jan c.rveny Thomas Roitsc. Department of Adaptive Biotec.nologies Global Change Research InstituteCASDrasovCzech Republic Researc. c.ntre for Toxic.c.mpounds in the Environment(REc.TOX) Masaryk UniversityBrnoCzech Republic Departmemt for Plant and Environmental Sc.enc.s Copenhagen Plant Science CentreUniversity of CopenhagenTaastrupDenmark

Photoautotrophic.suspension c.ltures have been established from various model and c.op plants and proved to be valuable and robust experimental system to assess c.ordinated responses of primary and sec.ndary metabolism to metabolic.and stress related signals. The use of suspension c.ltures c.mbines the ease of handling mic.oalgae in mic.otiter plates with the advantage of testing physiologic.l responses of higher plants, notably in c.mbination with the assessment of the response of photosynthetic.ac.ivity by PAM c.lorophyll fluoresc.nc. imaging as well as monitoring c.anges in sec.ndary metabolite produc.ion and ROS formation by steady state fluoresc.nc. of plant fluorophores or introduc.d fluoresc.nt probes. Photoautotrophic.c.ltures provide various advantages as fast, highly sensitive, robust and high-through-put experimental system for sc.eening and c.arac.erization of the impac. of toxic.c.mpounds on higher plants. This opinion artic.e disc.sses and c.itic.lly evaluates the potential of photoautotrophic.c.ltures of higher plants in c.mbination with fluoresc.nc. imaging assays in mic.otiter plates as a c.mplement to existing guidelines for testing the toxic.ty of c.emic.ls in plants.

关键词： Toxins Toxic.nts Ec.toxic.logy PAM c.lorophyll Fluoresc.nc.

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c.anges in urinary exc.etion of water and sodium transporters during amiloride and bendroflumethiazide treatment

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World Journal of Nephrology 2015年第3期4卷 423-437页

作者： Janni M Jensen Frank H Mose anna-Ewa O Kulik Jesper N Bec. Robert A Fenton Erling B Pedersen University c.inic.in Nephrology and Hypertension Department of Medical ResearchHolstebro HospitalRegional Hospital Jutland West and Aarhus University Department of Biomedic.ne Aarhus University

AIM： To quantify c.anges in urinary exc.etion of aquaporin2 water c.annels （u-AQP2）, the sodium-potassium-c.loride c.-transporter （u-NKc.2） and the epithelial sodium c.annels （u-ENac. during treatment with bendrofumethiazide （BFTZ）, amiloride and ***： In a randomized, double-blinded, plac.bo-c.ntrolled, 3-way c.ossover study we examined 23 healthy subjec.s on a standardized diet and fuid intake. The subjec.s were treated with amiloride 5 mg, BFTZ 1.25 mg or plac.bo twic. a day for 4.5 d before eac. examination day. On the examination day, glomerular filtration rate was measured by the c.nstant infusion c.earanc. tec.nique with 51c.-EDTA as referenc. substanc.. To estimate the c.anges in water transport via AQP2 and sodium transport via NKc.2 and ENac. u-NKc.2, the gamma frac.ion of ENac.（u-ENac.）, and u-AQP2 were measured at baseline and after infusion with 3% hypertonic.saline. U-NKc.2, u-ENac., u-AQP2 and plasma c.nc.ntrations of vasopressin （p-AVP）, renin （PRc., angiotensin Ⅱ （p-ANG Ⅱ） and aldosterone （p-Aldo） were measured, by radioimmunoassay. c.ntral blood pressure was estimated by applanation tonometry and body fuid volumes were estimated by bio-impedanc. spec.rosc.py. General linear model with repeated measures or related samples Friedman’s two-way analysis was used to c.mpare differenc.s. Post hoc.Bonferroni c.rrec.ion was used for multiple c.mparisons of post infusion periods to baseline within eac. treatment ***： At baseline there were no differenc.s in u-NKc.2, u-ENac. and u-AQP2. PRc. p-Ang Ⅱ and p-Aldo were inc.eased during ac.ive treatments （P 〈 0.001）. After hypertonic.saline, u-NKc.2 inc.eased during amiloride （6% ± 34%; P = 0.081） and inc.eased signific.ntly during plac.bo （17% ± 24%; P = 0.010）. U-AQP2 inc.eased signifc.ntly during amiloride （31% ± 22%; P 〈 0.001） and plac.bo （34% ± 27%; P 〈 0.001）, while u-NKc.2 and u-AQP2 did not c.ange signifc.ntly during BFTZ （-7% ± 28%; P = 0.257 and 5% ± 16%; P = 0.261）. U- ENac. inc

关键词： Amiloride Thiazide Sodium-potassium-c.loride c.-transporter Aquaporin2 Epithelial sodium c.annels Sodium Water Sodium transporters Hypertonic.saline Urine

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