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A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine

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Horticulture Research 2016年第1期3卷 302-313页

作者： Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks anna katharine mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun Horticulture Section School of Integrative Plant ScienceCornell UniversityGenevaNY 14456USA Bioinformatics Facility Cornell UniversityIthacaNY 14853USA Institute of Biotechnology Cornell UniversityIthacaNY 14853USA Plant Breeding and Genetics Section School of Integrative Plant ScienceCornell UniversityIthacaNY 14853USA Department of Horticultural Science University of MinnesotaSt PaulMN 55108USA Department of Food Science Cornell UniversityGenevaNY 14456USA Department of Food Science Cornell UniversityIthacaNY 14853USA USDA-ARS Grape Genetics Research Unit GenevaNY 14456USA Plant Science Department South Dakota State UniversityBrookingsSD 57007USA Plant Pathology and Plant-Microbe Biology Section School of Integrative Plant ScienceCornell UniversityGenevaNY 14456USA

Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to ***-generation sequencing and its derivative technologies have been used for genome-wide molecular marker *** bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision *** results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine *** vast majority of sequence reads(⩾99%)were from the targeted *** 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis.

关键词： crops breeding cultivar

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Current modalities of sickle cell disease management

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Blood Science 2020年第4期2卷 109-116页

作者： Adekunle Sanyaolu Ejoke Agiri Carl Bertram Latasha Brookes Jesy Choudhury Dorina Datt Amira Ibrahim anna Maciejko anna mansfield Jasmine Nkrumah Martina Williams Federal Ministry of Health AbujaNigeria Saint James School of Medicine AnguillaBWI

Sickle cell disease(SCD)affects nearly 100,000 people in the United States of America and the sickle gene is present in approximately 8%of black *** Africans,the prevalence of sickle cell trait(heterozygosity)is as high as 30%.While SCD occurs among varying racial and ethnic groups,it is more commonly prevalent in individuals of African or African-American *** inherited blood disorder causes varying symptoms and complications among affected children and adults and early diagnosis and treatment are essential to help reduce mortality *** there is no cure for SCD,management is vital to ***,there are different approaches in use to aid those living with SCD;thus,this paper provides insight into the current methods that are implemented in the management and maintenance of this disease.

关键词： Hemoglobin S(HbS) Sicklecellanemia Sicklecelldisease(SCD) UnitedStatesofAmerica

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Microcomputer hardware, software, and troubleshooting for engineering and technology / James L. Antonakos, Kenneth C. mansfield.

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2000年

作者： James L. Antonakos Kenneth C. mansfield.

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What Is Politics？--A Dialogue between Profs. Dongfeng Tao and Harvey Claflin mansfield

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2013年第4期7卷 706-712页

作者： Dongfeng Tao Harvey Claflin mansfield Harvard Univ Ctr Govt & Int Studies Room N417 Cambridge MA 02138 USA

Co-authors： Dongfeng Tao and Harvey Claflin mansfield Oral interpreted by Yue Hou （Massachusetts Institute of Technology） Transcripted by Peter Jones （New York University）

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Endo-β-1,4-glucanases impact plant cell wall development by influencing cellulose crystallization

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Journal of Integrative Plant Biology 2015年第4期57卷 396-410页

作者： Magdalena Glass Sarah Barkwill Faride Unda Shawn D.mansfield Department of Wood Science University of British Columbia

Cell walls are vital to the normal growth and development of plants as they protect the protoplast and provide rigidity to the stem. Here, two poplar and Arabidopsis orthologous endoglucanases, which have been proposed to play a role in secondary cell wall development, were examined. The class B endoglucanases, Pt GH9B5 and At GH9B5, are secreted enzymes that have a predicted glycosylphosphatidylinositol anchor, while the class C endoglucanases, Pt GH9C2 and At GH9C2, are also predicted to be secreted but instead contain a carbohydrate-binding *** poplar endoglucanases were expressed in Arabidopsis using both a 35 S promoter and the Arabidopsis secondary cell wall-specific Ces A8 promoter. Additionally, Arabidopsis t-DNA insertion lines and an RNAiconstruct was created to downregulate At GH9C2 in Arabidopsis. All of the plant lines were examined for changes in cell morphology and patterning, growth and development, cell wall crystallinity, micro fibril angle, and proportion of cell wall carbohydrates. Misregulation of Pt GH9B5/At GH9B5 resulted in changes in xylose content, while misregulation of Pt GH9C2/At GH9C2 resulted in changes in crystallinity, which was inversely correlated with changes in plant height and rosette diameter. Together, these results suggest that these endoglucanases affect secondary cell wall development by contributing to the cell wall crystallization process.

关键词： Cellulose cell walls crystallinity endoglucanase expansion glucose xylose

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Psychotropic Medication Refusal:Reasons and Patients’Perception at a Secure Forensic Psychiatric Treatment Centre

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Journal of Forensic Science and Medicine 2016年第1期2卷 12-17页

作者： Olajide O Adelugba mansfield Mela Inam U Haq Regional Psychiatric Center University of SaskatchewanSaskatoonSaskatchewanCanada Department of Psychiatry University of SaskatchewanSaskatoonSaskatchewanCanada

Poor adherence to prescribed medication regimens can undermine the effectiveness of *** study was conducted to determine the demographic profile of forensic psychiatric inpatients refusing medications and to identify the reasons for *** were collected through interviews using a questionnaire including Drug Attitude Inventory‑*** refusal was more common among Aboriginals(68%,n=34)than Caucasians(32%,n=16)and was highest among the patients 21–30 years of age(44%,n=22).Antisocial personality disorder and substance use disorder featured prominently among patients refusing *** main reasons for medication refusal were inconvenience(34%,n=17)followed by side effects(22%,n=11),ineffective medication(20%,n=10),illness‑related(16%,n=8),and no reasons(8%,n=6).Antipsychotic medications topped the list of the major classes of medications refused followed by Antidepressants and Mood Stabilizers.

关键词： Forensic medication nonadherence psychiatry refusal

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Manipulating Chladni Patterns of Ferromagnetic Materials by an External Magnetic Field

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Sound & Vibration 2021年第3期55卷 235-240页

作者： Kenneth R.Podolak Vihan A.W.Wickramasinghe Gareth A.mansfield Alex M.Tuller State University of New York Plattsburgh12901USA

Ernst Chladni is called the father of acoustics for his work,which includes investigating patterns formed by vibrating *** these patterns helps research involving standing waves and other harmonic behaviors,including studies of single electron orbits in *** experiment vibrates circular plates which result in well-known *** to traditional experiments that used sand or salt,we use magnetic materials,namely iron filings and nickel *** then manipulate the patterns by applying a localized external magnetic field to one of the rings that moves a segment of the magnetic material in that ring to the next inner *** results show a significant decrease in magnetic field necessary to move the magnetic material at higher frequencies as well as a significant decrease in the magnetic field required to move the magnetic material as nickel powder is substituted for iron filings while keeping the mass constant.

关键词： Chladni ferromagnetism acoustics

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Effects of intracellular application of CsCL on action potential and contractio,

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中国药理学与毒理学杂志 1995年第1期9卷 73-74页

作者： CUI Yi Derek A TERRAR(University Department of Pharmacology, mansfield jRoad,Oxford OX13QT,UK) Univ. Department of Pharmacology Mansfield Road Oxford OX1 3QT United Kingdom

ＥｆｆｅｃｔｓｏｆｉｎｔｒａｃｅｌｌｕｌａｒａｐｐｌｉｃａｔｉｏｎｏｆＣｓＣｌｏｎａｃｔｉｏｎＰｏｔｅｎｔｉａｌａｎｄｃｏｎｔｒａｃｔｉｏｎｏｆｉｓｏｌａｔｅｄｇｕｉｎｅａ－ｐｉｇｖｅｎｔｒｉｃｕｌａｒｃｅｌｌｓＣＵ... 详细信息

ＥｆｆｅｃｔｓｏｆｉｎｔｒａｃｅｌｌｕｌａｒａｐｐｌｉｃａｔｉｏｎｏｆＣｓＣｌｏｎａｃｔｉｏｎＰｏｔｅｎｔｉａｌａｎｄｃｏｎｔｒａｃｔｉｏｎｏｆｉｓｏｌａｔｅｄｇｕｉｎｅａ－ｐｉｇｖｅｎｔｒｉｃｕｌａｒｃｅｌｌｓＣＵＩＹｉ；ＤｅｒｅｋＡＴＥＲＲＡＲ（...

关键词： myocardium, sarcoplasmicreticulum, ion channels, K+ contraction, cesium

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Sun and Moon

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英语学习 1994年第3期 3-9页

作者： Katherine mansfield 乐安

In the afternoon the chairs came,a whole big cart full of lit-tle gold ones with their legs in the *** then the *** you stared down from the balcony at the people car-rying them the flower pots looked like funny awfully nice hatsnodding up the *** thought they were *** said:"***'s aman wearing a palm on his head."But she never knew the differ-

关键词： down over

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帕博利珠单抗单药治疗PD-L1阳性伴脑转移的非小细胞肺癌患者的疗效：KEYNOTE-001、010、024和042研究的汇总分析

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中国肺癌杂志 2022年第9期25卷 701-712页

作者： Aaron S. mansfield Roy S. HERBST Gilberto de CASTRO Jr. Rina HUI Nir PELED Dong-Wan KIM Silvia NOVELLO Miyako SATOUCHI Yi-Long WU Edward B.GARON Martin RECK Andrew G.ROBINSON Ayman SAMKaRI Bilal PIPERDI Victoria EBIANA Jianxin LIN Tony S.K.MOK 黎扬斯 Division of Medical Oncology Mayo Clinic Yale Comprehensive Cancer Center Yale University School of Medicine Instituto do Cancer do Estado de S?o Paulo Westmead Hospital and the University of Sydney Soroka Cancer Center Ben Gurion University Seoul National University Hospital Seoul National University College of Medicine Azienda Ospedaliero-Universitaria San Luigi Gonzaga University of Turin Hyogo Cancer Center Guangdong Lung Cancer Institute Guangdong Provincial People's Hospital and Guangdong Academy of Medical Sciences David Geffen School of Medicine at the University of California Los Angeles Lung Clinic Grosshansdorf Airway Research Center North German Center of Lung Research Cancer Centre of Southeastern Ontario at Kingston General Hospital Merck & Co. Inc. State Key Laboratory of Translational Oncology Chinese University of Hong Kong 广东省人民医院广东省人民医院肿瘤医院广东省肺癌研究所

背景与目的我们回顾性评估了程序性死亡配体1（programmed death ligand 1, PD-L1）阳性非小细胞肺癌（non-small cell lung cancer, NSCLC）患者的预后，以比较基线（即入组时）脑转移与帕博利珠单抗及化疗的疗效关系。方法我们汇总... 详细信息

背景与目的我们回顾性评估了程序性死亡配体1（programmed death ligand 1, PD-L1）阳性非小细胞肺癌（non-small cell lung cancer, NSCLC）患者的预后，以比较基线（即入组时）脑转移与帕博利珠单抗及化疗的疗效关系。方法我们汇总分析了KEYNOTE-001（NCT01295827）、KEYNOTE-010（NCT01905657）、KEYNOTE-024（NCT02142738）和KEYNOTE-042（NCT02220894）研究中既往接受过治疗或未接受过的PD-L1阳性[肿瘤细胞阳性比例评分（tumor proportion score, TPS）≥1%]的晚期或转移性NSCLC患者的数据。患者接受帕博利珠单抗（2 mg/kg,10 mg/kg，或每3周200 mg或每2周10 mg/kg）治疗；而化疗是除KEYNOTE-001外所有研究的对照组。所有研究都纳入了既往接受过治疗的稳定脑转移的患者。结果共计纳入3,170例患者，基线时，293例（9.2%）患者伴有脑转移，而2,877例（90.8%）是无脑转移患者；数据截止时，中位（范围）随访时间为12.9（0.1-43.7）个月。与化疗相比，帕博利珠单抗可改善基线伴有脑转移及无脑转移患者的总生存期：PD-L1 TPS≥50%[分别为0.67（95%CI:0.44-1.02）和0.66（95%CI:0.58-0.76）]和PD-L1 TPS≥1%[分别为0.83（95%CI:0.62-1.10）和0.78（95%CI:0.71-0.85）]。无论是否有脑转移，与化疗相比，接受帕博利珠单抗治疗患者的无进展生存期更长，客观缓解率更高，缓解持续时间更长。在脑转移患者中，帕博利珠单抗与化疗的治疗相关不良事件发生率分别为66.3%和84.4%，在无脑转移患者中分别为67.2%和88.3%。结论对于既往未接受治疗或接受过治疗的PD-L1阳性晚期/转移性NSCLC患者，无论基线时是否存在治疗后稳定的脑转移，帕博利珠单抗单药治疗显示出明显的疗效改善，且与化疗相比，不良事件更少。

关键词：帕博利珠单抗脑转移化疗非小细胞肺癌 PD-L1

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