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Journal of Forestry Research 2021年第1期32卷 249-262页

作者： Wei Tang anna Y.Tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA 2 Stadium Drive University of North Carolina at Chapel HillChapel HillNC 27514USA

Transcription factors can be used to engineer plants for enhanced ***,the mechanism(s)by which the C2H2-type zinc fi nger transcription factor enhances pathogen resistance in cells is not fully ***,Agrobacterium tumefaciens carrying the gene for Arabidopsis thaliana cysteine2/histidine2-type transcription factor 6(ZAT6)was used to engineer rice(Oryza sativa L.),cotton(Gossypium hirsutum L.),and slash pine(Pinus elliottii Engelm.)to generate transgenic cell *** cells were then inoculated with the pathogenic bacterium Pseudomonas *** to the control,cell viability of transgenic cells increased 39–47%and growth rate increased 9–15%by 7 days after inoculation in rice,cotton and slash *** phosphatase activity and alkaline phosphatase activity and transcript levels of Ca 2+-dependent protein kinase genes OsCPK1,OsCPK2,OsCPK6,and OsCPK8 and mitogen-activated protein kinase genes OsMAPK1,OsMAPK2,OsMAPK3,and OsMAPK8 increased signifi cantly in transgenic rice cells by 3 day after inoculation,and extracellular pH had decreased by 10–14%by 96 min after inoculation in transgenic rice,cotton and slash pine *** results suggest that ZAT6 enhances *** resistance in plant cells by modulating transcription of CPK and MAPK and oxidase activity.

关键词： Ca^2+-dependent protein kinase Mitogenactivated protein kinase Oxidase Pathogen Pinus

Remembering,Forgetting,and Absencing Disasters in the Post-disaster Recovery Process

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International Journal of Disaster Risk Science 2020年第3期11卷 287-299页

作者： Charlotte Monteil Jenni Barclay anna Hicks Lancaster Environment Center Lancaster UniversityBailrigg LAI 4YWUK School of Environmental Sciences University of East AngliaNorwich NR47TJUK Edinburgh Office British Geological SurveyEdinburgh EH 144BAUK

Sustainable post-disaster recovery implies learning from past experience in order to prevent recreating forms of *** construction supports both the healing process and redevelopment ***,memory of disaster results from the balance between remembering,forgetting,and absencing elements of the disaster,and can be both a tool and an obstacle to sustainable *** explore here how collective memory is built in a post-disaster context to respond to the needs of this critical period,and how it shapes *** ethnographic study,conducted between 2015 and 2017,explores the recovery processes in Montserrat,a small Caribbean island affected by an extended volcanic crisis from 1995 to *** this study does not give tangible solutions for disaster risk reduction in a post-disaster context,it highlights potential obstacles for learning from a disaster and how they may be *** argue that it is crucial to acknowledge evolving collective memory in order to implement effective measures for preserving and sharing a shared understanding of disaster across generations and social groups in a way that supports disaster risk *** also maintain that acknowledging the dilemma faced by authorities and disaster management agencies during a period of conflicting needs may encourage the reconsideration of risk framing,and hence reveal how to improve implementation of disaster risk reduction measures.

关键词： Absencing risk information Disaster remembering Forgetting processes Montserrat Post-disaster recovery

KRAB-ZFPs and cancer stem cells

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Genes & Diseases 2023年第5期10卷 1820-1832页

作者： anna Olechnowicz Urszula Oleksiewicz Marta Machnik Department of Cancer Immunology Poznan University of Medical SciencesPoznan 60-806Poland Department of Histology and Embryology Poznan University of Medical SciencesPoznan 60-781Poland Doctoral School Poznan University of Medical SciencesPoznan 60-812Poland Department of Cancer Diagnostics and Immunology Greater Poland Cancer CentrePoznan 61-866Poland

Studies on carcinogenesis continue to provide new information about different disease-related *** others,much research has focused on the involvement of cancer stem cells(CSCs)in tumor initiation and *** the similarities and dif-ferences between CSCs and physiological stem cells(SCs)allows for a better understanding of cancer ***,it was shown that stem cell identity is partially governed by the Kruppel-associated box domain zinc finger proteins(KRAB-ZFPs),the biggest family of tran-scription *** KRAB-ZFP factors exert a known effect in tumor cells,acting as tumor suppressor genes(TSGs)or oncogenes,yet their role in CSCs is still poorly ***,we review recent studies regarding the influence of KRAB-ZFPs and their cofactor pro-tein TRIM28 on CSCs phenotype,stemness features,migration and invasion potential,metas-tasis,and expression of parental markers.

关键词： Cancer Cancer stem cell Epigenetic KRAB-ZFP Transcription factors

Three-dimensional in vitro models of neuromuscular tissue

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Neural Regeneration Research 2022年第4期17卷 759-766页

作者： Paolo Raffa Maria Easler anna Urciuolo Institute of Pediatric Research IRP PadovaItaly Molecular Medicine Department University of PadovaPadovaItaly

Skeletal muscle is a dynamic tissue in which homeostasis and function are guaranteed by a very defined three-dimensional organization of myofibers in respect to other nonmuscular components,including the extracellular matrix and the nervous *** particular,communication between myofibers and the nervous system is essential for the overall correct development and function of the skeletal muscle.A wide range of chronic,acute and genetic-based human pathologies that lead to the alteration of muscle function are associated with modified preservation of the fine interaction between motor neurons and myofibers at the neuromuscular *** advancements in the development of in vitro models for human skeletal muscle have shown that three-dimensionality and integration of multiple cell types are both key parameters required to unveil pathophysiological relevant ***,we describe recent achievement reached in skeletal muscle modeling which used biomaterials for the generation of three-dimensional constructs of myotubes integrated with motor neurons.

关键词： 3D organization bioengineering biomaterials motor neurons neuromuscular junction skeletal muscle models

What is attractive rural landscape?Differences in the social and expert assessment of the changes in the rural landscape of the Carpathian region in Poland with regard to the need of its protection

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Journal of Mountain Science 2023年第2期20卷 501-515页

作者： Agata GAJDEK Barbara KRUPA anna NOWAK Department of Landscape Architecture Institute of Agricultural SciencesEnvironmental Protection and DevelopmentCollege of Natural SciencesUniversity of Rzeszowul.Ćwiklińskiej 2PL-35-601 RzeszówPoland

Contemporary villages of the mountain region are subject to uncontrolled structural and spatial transformations,which cause deformation of centuries-old spatial systems of high cultural and natural *** aim of the study is to confront the opinions of the inhabitants and experts regarding the condition of the cultural landscape of the villages in south-eastern *** area belongs to the Carpathian region of Central *** historical and economic conditions of the studied region,related to the functioning in the post-war period,and then its breakdown and the development of the free market economy,constitute an interesting background for the proposed *** communities still remembering the period of difficulties related to the period of systemic transformations,are currently experiencing a relative prosperity,many difficulties related to the period of systemic transformations,are currently experiencing a relative prosperity,which is also expressed in a completely new,previously unknown way of managing the *** implemented in villages are associated by the inhabitants with the improvement of the standards and quality of *** assess them rather *** expert assessment of these landscape transformations indicates their negative dimension and the risk of losing timeless *** discrepancy in the assessment of experts and local residents creates difficulties in the protection of the rural ***,high-quality visual landscape features among rural residents is necessary from the point of view of its multi-faceted and effective *** initiatives and actions in the field of industry policy should play a significant role in this respect by consolidating the images of a harmonious landscape in the public awareness.

关键词： Polish village Landscape attractiveness Cultural landscape Spatial systems

Overexpression of Arabidopsis thaliana malonyl-CoA synthetase gene enhances cold stress tolerance by activating mitogen-activated protein kinases in plant cells

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Journal of Forestry Research 2021年第2期32卷 741-753页

作者： Wei Tang anna Y.Tang College of Arts and Science East Carolina UniversityGreenvilleNC 27858USA 101 Stadium Drive University of North Carolina at Chapel HillChapel HillNC 27514USA

Malonyl-CoA synthetases may modulate cell responses to abiotic stress by regulating stress-related signaling transduction pathways or activating expression of transcription ***,the molecular mechanism of cold stress tolerance enhanced by malonyl-CoA synthetase is not fully ***,we report that overexpression of the Arabidopsis thaliana malonyl-CoA synthetase gene AAE13.1 resulted in increased cell viability and growth rate and decreased thiobarbituric acid reactive substances under cold stress in rice(Oryza sativa L.),tobacco(Nicotiana tabacum),and slash pine(Pinus elliottii Engelm.).AAE13.1 was associated with cold stress tolerance by increasing the activity of ascorbate peroxidase,catalase,polyphenol oxidase,and peroxidase and the accumulation of acid phosphatase and alkaline *** six rice mitogenactivated protein kinase(MAPK)genes examined,AAE13.1 overexpression increased the expression of OsMAPK genes during cold ***13.1 activated expression of stressresponse genes OsMAPK1,OsMAPK2,and OsMAPK3,indicating that AAE13.1 enhances cold stress tolerance by regulating expression of MAPK genes in plant *** results increase our understanding of cold stress tolerance in species of monocotyledons,dicotyledons,and gymnosperms.

关键词： Cold stress Malonyl-CoA synthetase gene MAPK transcription factor Phosphatase Pinus

On Desideratum for B2C E-Commerce Reputation Systems

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Journal of Computer Science & Technology 2009年第5期24卷 820-832页

作者： anna Gutowska Andrew Sloane Kevan A.Buckley School of Computing and Information Technology University of WolverhamptonWolverhamptonWV1 1SBU.K.

This paper reviews existing approaches to reputation systems, their constraints as well as available solutions. Furthermore, it presents and evaluates a novel and comprehensive reputation model devoted to the distributed reputation system for Business-to-Consumer （B2C） E-commerce applications that overcomes the discussed drawbacks. The algorithm offers a comprehensive approach as it considers a number of issues that have a bearing on trust and reputation such as age of ratings, transaction value, credibility of referees, number of malicious incidents, collusion and unfair ratings. Moreover, it also extends the existing frameworks based on information about past behaviour, with other aspects affecting online trading decisions which relate to the characteristic of the providers, such as existence of trustmark seals, payment intermediaries, privacy statements, security/privacy strategies, purchase protection/insurance, alternative dispute resolutions as well as the existence of first party information.

关键词： decision support distributed systems electronic commerce online information services

Contribution of precursor compounds to the release of per-and polyfluoroalkyl substances(PFASs) from waste water treatment plants(WWTPs)

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Journal of Environmental Sciences 2017年第11期29卷 80-90页

作者： Ulrika Eriksson Peter Haglund anna K?rrman Man-Technology-Environment (MTM) Research Centre School of Science and Technology ?rebro University Department of Chemistry Umea University

Per-and polyfluoroalkyl substances（PFASs） are ubiquitous in sludge and water from waste water treatment plants, as a result of their incorporation in everyday products and industrial processes. In this study, we measured several classes of persistent PFASs,precursors, transformation intermediates, and newly identified PFASs in influent and effluent sewage water and sludge from three municipal waste water treatment plants in Sweden, sampled in 2015. For sludge, samples from 2012 and 2014 were analyzed as *** of precursors in sludge exceeded those of perfluoroalkyl acids and sulfonic acids（PFCAs and PFSAs）, in 2015 the sum of polyfluoroalkyl phosphoric acid esters（PAPs） were 15–20 ng/g dry weight, the sum of fluorotelomer sulfonic acids（FTSAs） was 0.8–1.3 ng/g,and the sum of perfluorooctane sulfonamides and ethanols ranged from non-detected to 3.2 ng/g. Persistent PFSAs and PFCAs were detected at 1.9–3.9 ng/g and 2.4–7.3 ng/g dry weight, respectively. The influence of precursor compounds was further demonstrated by an observed substantial increase for a majority of the persistent PFCAs and PFSAs in water after waste water treatment. Perfluorohexanoic acid（PFHxA）, perfluorooctanoic acid（PFOA）, perfluorohexane sulfonic acid（PFHxS）, and perfluorooctane sulfonic acid（PFOS）had a net mass increase in all WWTPs, with mean values of 83%, 28%, 37% and 58%,respectively. The load of precursors and intermediates in influent water and sludge combined with net mass increase support the hypothesis that degradation of precursor compounds is a significant contributor to PFAS contamination in the environment.

关键词： diPAPs FTSAs Waste water Daily discharge PFCA precursor

Grafting affects yield and phenolic prof ile of Solanum melongena L. landraces

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Journal of Integrative Agriculture 2016年第5期15卷 1017-1024页

作者： L Sabatino G Iapichino A Maggio E D’ anna M Bruno F D’ anna Department of Agricultural and Forest Sciences University of Palermo Department of Biological Chemical and Pharmaceutical Sciences and Technologies University of Palermo Regional Centres for Agricultural Technologies (Ce.R.T.A.) University of Foggia

The influence of grafting on agronomical and qualitative characteristics of four Sicilian eggplant landraces was investigated. Grafted and ungrafted plants were compared in the open field in the northern coast of Sicily. Solanum torvum seedlings were used as rootstock. Regardless of genotypes tested, grafting significantly increased total fruit production, marketable production, and number of marketable fruits, but did not affect weight of marketable fruits and waste production. Landrace 2（Sciacca）, with black epidermal tissue and pyriform fruit shape, when grafted onto S. torvum not only gave a higher yield performance than ungrafted plants, but also showed a higher phenolic antioxidant content. Landrace 4（Sicilia）, with black epidermal tissue and small cylindrical fruits also benefited, when grafted onto S. torvum, from a substantial increase in antioxidant fruit content. As consumers＇ demand for fruits and vegetables rich in compounds important for human health is steadily increasing, these landrace/rootstock combinations should deserve more attention by plant nurseries involved in grafted seedling production and interested in the valorization and conservation of eggplant biodiversity.

关键词： eggplant propagation technique yield polyphenols HPLC analysis

The electrochemical detection of bioterrorism agents: a review of the detection, diagnostics, and implementation of sensors in biosafety programs for Class A bioweapons

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Microsystems & Nanoengineering 2021年第1期7卷 21-39页

作者： Connor O’Brien Kathleen Varty anna Ignaszak Department of Chemistry University of New Brunswick30 Dineen DriveFrederictonNB E3B 5A3Canada

During the past year,disease has shown us the iron grip it can hold over a population of *** systems can be overwhelmed,economies can be brought into recession,and many people can be harmed or *** weaponized,diseases can be manipulated to create a detriment to health while becoming an economic burden on any *** is consequently prudent that easy detection of bioweapons is available to governments for protecting their *** sensing displays many distinct advantages,such as its low limit of detection,low cost to run,rapid generation of results,and in many instances *** therefore present a wide array of electrochemical sensing platforms currently being fabricated,a brief summary of Class A bioweapons,and the potential future of bioweapon detection and biosafety.

关键词： electrochemical instance programs