检索结果-南通市图书馆

Species Identification by Means of Mitochondrial Cytochrome <i>b</i>DNA Sequencing in Processed Anchovy, Sardine and Tuna Products

引用

Food and Nutrition Sciences 2018年第4期9卷 369-375页

作者： anna cutarelli Giorgio Galiero Federico Capuano Federica Corrado Istituto Zooprofilattico Sperimentale del Mezzogiorno Napoli Italy

Identifying the contents of processed food products is essential to correct labelling. In processed foodstuffs, species identification through morphological analysis is difficult. Several factors hinder the identification of fish species in processed foods: proteins or other materials subjected to analysis may be denatured during heat treatments;the presence of other ingredients (e.g., olive and other vegetable oils) may interfere with the analysis. Consequently, possible frauds perpetrated by replacing valuable species with less precious ones may go undetected. In most processed samples (e.g. canned products), DNA is degraded into small fragments, which considerably reduces the sensitivity of molecular analysis. The main goal of our research was to develop an analytical method able to identify fish species in highly processed products, such as canned fish. The assay was developed by combining an effective method of DNA recovery from samples with the detection of small-sized sequences of the mitochondrial Cytb gene. This method appears particularly suitable when morphological characterization is difficult, to carry out such as in canned products where DNA is degraded or present in small quantities. We have analyzed 60 samples of seafood commercial products identifying 3 different genera and five different species. All analyzed samples revealed a correct species declaration, for one sample we highlighted important commercial fraud. We also used bio-informatic identification systems for the Sequence Alignment and the construction of phylogenetic tree to better confirm the revealed fraud.

关键词： Species Identification Processed Seafood Commercial Fraud Dna Sequencing Cytochrome b (Cytb)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Role of Ozone Carried by Liposomes in the Therapy of Infectious and Skin-Regenerating Ocular Surface

引用

Journal of Biomedical Science and Engineering 2019年第7期12卷 347-353页

作者： anna cutarelli Gianpaolo Carlini Federica Sarno Angela Nebbioso Francesca Garofalo Lucia Altucci Federica Corrado Istituto Zooprofilattico Sperimentale del Mezzogiorno di Portici (NA) Naples Italy Pathology Center of the Cornea and Ocular Surface-U.O. of Ophthalmology P.O. San Giovanni Bosco Naples Italy Department of Medicine of Precision University of Studi della Campania Luigi Vanvitelli Naples Italy

Effect of the ozonated oil in liposomes: they act against Staphylococcus aureus and Pseudomonas aeruginosa is among the most frequent eye pathogens and causes acute and chronic infections of the ocular surface. Staphylococcus aureus and Pseudomonas aeruginosa were tested in this study. The bacterial suspension of Staphylococcus aureus was diluted to obtain 150 CFU/ml. The bacterial suspension of Pseudomonas aeruginosa was diluted to obtain 15 CFU/ml. Various volumes of liposome-vehiculated ozonated oil were added (400 μl, 200 μl, 100 μl and 50 μl) to 100 μl of bacterial suspension;both of Staphylococcus aureus and Pseudomonas aeruginosa are incubated at 37?C for 10 minutes. The cytotoxicity of liposome-vehiculated ozonated oil was analysed at the University of Campania “Luigi Vanvitelli”, Department of Precision Medicine. The HaCaT epidermal keratinocyte cell line (ATCC, USA) was grown in Dulbecco’s Modified Eagle Medium (Euroclone) with the addition of 10% foetal bovine serum (FBS) (Euroclone), 2 mM of L-glutamine (Euroclone) and antibiotics (100 U/ml penicillin, 100 g/ml streptomycin) (Euroclone). The microbiological results clearly show the antimicrobial efficacy of liposome-vehiculated ozonated oil against bacterial strains such as Staphylococcus aureus and Pseudomonas aeruginosa. Furthermore, the studies carried out in vitro on the keratinocyte line showed how ozonated oil in liposomes does not evidence any cell toxicity, and that after 3 days of treatment, it promotes cell growth compared to the positive control.

关键词： Ozonated Oil in Liposomes Staphylococcus aureus Pseudomonas aeruginosa Cytotoxicity MTT Assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Isolation of <i>Bacillus cereus</i>in a Facility Preparing School Meals

引用

Food and Nutrition Sciences 2020年第3期11卷 186-193页

作者： Francesca Garofalo anna cutarelli Rita Nappi Assunta De Lella Marcella Palomba Salvatore Capo Angela Michela Immacolata Montone Loredana Biondi Federica Corrado Food Inspection Department Istituto Zooprofilattico Sperimentale del Mezzogiorno Portici Italy

Food safety is a fundamental requirement in mass catering, as large numbers of meals are served each day to potentially vulnerable consumers, such as children. Food Business Operators implement plans for the microbiological monitoring of the meals prepared and served in the catering sector, and for the swab-sampling of surfaces. From January 2018 to June 2019, our laboratory analyzed both food and swab samples from four catering facilities. Considering the EFSA 2018 data, we specifically focused on samples analyzed for Bacillus cereus. Our data substantially showed episodic contamination due to a piece of equipment that is not usually subjected to microbiological control, thus suggesting that every aspect should be scrutinized in order to identify critical points. While Bacillus cereus is widespread in nature and common in soil, it is adapted for growth in the intestinal tracts of insects and mammals. It is often present in a variety of foods, and may cause an emetic or a diarrheal type of food-associated illness. B. cereus produces several toxins. Multiplex PCR enables seven toxin genes to be detected (hblC, hblD, hblA, nheA, nheB, nheC and cytK).

关键词： Bacillus cereus Food-Borne Disease School Meals Enterotoxin Genes

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cyclicity of the Lower Buntsandstein in the eastern part of the Central European Basin:implications for Early Triassic palaeogeography and for geochronological calibration

引用

Journal of Palaeogeography 2024年第2期13卷 252-292页

作者： anna Becker Polish Geological Institute-National Research Institute 4 Rakowiecka str.00-975 WarsawPoland

Lower Buntsandstein small—scale cycles recognized in the Central European Basin(CEB) are readily used for astrochronological calibration of the Early Triassic time scales,although they are not well studied *** borehole sections from the eastern part of the CEB,forming a south-north transect perpendicular to the basin axis,were studied in terms to better understand the depositional history of the Lower Buntsandstein developed as the Baltic Formation in the studied Polish part of the *** sedimentary facies were recognized,based on lithological and sedimentological investigations of 655.7 m of drill cores from the Otyń IG 1,Gorzów Wielkopolski IG 1 and Kamień Pomorski IG 1 *** facies can be grouped according to a lithological criterion into facies of predominantly siliciclastic lithology(including 9facies types) and facies of predominantly carbonate lithology(including 2 facies types).Facies analysis allowed to distinguish 8 facies associations of:1) alluvial fan,2) playa,3) sandy-muddy coastal plain,4)embayment and distal delta,5) lagoon,6) sand bars or shoals,7) ooidal shoals or bars,and 8) ***-upward(DC) and shallowing-upward(SC) sedimentary cycles were recognized in parts of the Baltic Formation,with DCs dominating in the southernmost located Otyń IG 1,and SCs dominating in Gorz ow Wielkopolski IG 1,located in the axial part of the *** cycles are very *** statistical significance of the sedimentary cycles is relatively low according to Markov-chain analysis conducted with the phpSedistat software of Stanova et al.(2009).Analysis of small—scale cycles performed on well logs of investigated boreholes and additional boreholes in the vicinity of Otyń IG 1,implementing the earlier study of Becker(2005),showed that the correlation of well-log cycles(GR cycles) and sedimentary cycles is not *** cycles can be roughly correlated with lithological carbonate—siliciclastic cycles(c-s cycles),refle

关键词： Triassic Lower Buntsandstein Cyclicity Central European Basin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Each coin has 2 sides: a positive role of alien Potamopyrgus antipodarum (Grey, 1843) snails in reducing the infection of native lymnaeids with trematodes

引用

Current Zoology 2024年第2期70卷 262-269页

作者： anna Cichy anna Stanicka Elzbieta Zbikowska Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in TorunLwowska 187-100 TorunPoland

The change in the distribution of organisms in freshwater ecosystems due to natural or manmade processes raises the question of the impact of alien species on local *** most studies indicate a negative effect,the positive one is more difficult to discern,espe-cially in multispecies systems,including hosts and *** purpose of the study was to check whether the presence of an alien host,Potamopyrgus antipodarum,reduces the intensity of Echinoparyphium aconiatum metacercariae in a native host,Radix *** additionally tested the impact of water temperature and the biomass of the alien host on the dilution *** experimentally studied(1)the lifespan of echinostome cercariae in different temperatures,(2)the infectivity of cercariae toward the alien host and native host,and(3)the impact of different biomass of the alien host on the intensity of metacercariae in the native *** found that cercarial survival and infectivity were tem-perature ***,cercarial survival decreased with increasing temperature,contrary to cercarial *** cercariae entered the renal cavity of both the native host and alien host,and successfully transformed into *** number of metacercariae in the native host decreased with the increasing biomass of the alien *** results indicate that lymnaeids may benefit from the co-occur-rence with P antipodarum,as the presence of additional hosts of different origins may reduce the prevalence of parasites in native ***,the scale of the dilution effect depends not only on the increased spectrum of susceptible hosts but also on the other variables of the environment,including water temperature and host density.

关键词： biological invasion density dilution effect echinostomes Radix spp. temperature.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

WNT/β-catenin signaling in hepatocellular carcinoma:The aberrant activation,pathogenic roles,and therapeutic opportunities

引用

Genes & Diseases 2024年第2期11卷 727-746页

作者： anna Gajos-Michniewicz Malgorzata Czyz Department of Molecular Biology of Cancer Medical University of LodzLodz 92-215Poland

Hepatocellular carcinoma(HCC)is a liver cancer,highly heterogeneous both at the histopathological and molecular *** arises from hepatocytes as the result of the accumulation of numerous genomic alterations in various signaling pathways,including canonical WNT/β-catenin,AKT/mTOR,MAPK pathways as well as signaling associated with telomere maintenance,p53/cell cycle regulation,epigenetic modifiers,and oxidative *** role of WNT/β-catenin signaling in liver homeostasis and regeneration is well established,whereas in development and progression of HCC is extensively ***,we review recent advances in our understanding of how WNT/β-catenin signaling facilitates the HCC development,acquisition of stemness features,metastasis,and resistance to *** outline genetic and epigenetic alterations that lead to activated WNT/β-catenin signaling in *** discuss the pivotal roles of CTNNB1 mutations,aberrantly expressed non-coding RNAs and complexity of crosstalk between WNT/β-catenin signaling and other signaling pathways as challenging or advantageous aspects of therapy development and molecular stratification of HCC patients for treatment.

关键词： Cancer stem cells CTNNB1 mutations Liver cancer Non-coding RNA Tumor heterogeneity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Double-check in Lizard Age Estimation:Use of Phalanx Bone and Keratin Claw Sheath Lamellas

引用

Asian Herpetological Research 2024年第1期15卷 59-61页

作者： Eduard GALOYAN Natalia SOPILKO anna KOVALYEVA anna CHAMKINA A.N.Severtsov Institute of Ecology and Evolution Moscow 119071Russia Lomonosov Moscow State University Leninskie Gori 1Moscow 119991Russia

Dear Editor,The problem of age estimation in amphibians and reptiles with annual fluctuations of growth pattern has been considered to be mostly solved since the skeletochronological method was introduced(Kleinenberg and Smirina,1969).This method is based on counting the number of lines of arrested growth(LAGs)—cyclical growth marks that are usually formed annually and characterized by different optical aspects within the tubular bones.

关键词： estimation method usual

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Polymerization of 1,3-Butadiene with Catalysts Based on Cobalt Dichloride Complexes with Aminophosphines: Switching the Regioselectivity by Varying the MAO/Co Molar Ratio

引用

Chinese Journal of Polymer Science 2024年第4期42卷 501-510页

作者： Giovanni Ricci anna Sommazzi Francesco Masi Alessandra Forni CNR-Istituto di Scienze e Tecnologie Molecolari“Giulio Natta”(SCITEC) via A.Corti 1220133MilanoItaly Viale Giovanni XXIII 34 I-28100NovaraNOItaly Via Galvani 7 I-26866Sant’Angelo LodigianoLOItaly CNR-Istituto di Scienze e Tecnologie Molecolari“Giulio Natta”(SCITEC) via C.Golgi 1920133MilanoItaly

Several novel cobalt dichloride complexes with amino-phosphine bidentate ligands were synthesized and *** some of them single crystals were obtained and their molecular structure was determined by X-ray diffraction *** the complexes were then used in combination with MAO for the polymerization of 1,3-butadiene,obtaining polymers with different structures(i.e.,predominantly 1,2 or cis-1,4)mainly depending on the type of ligand and on the MAO/Co molar *** behavior of these novel catalysts was compared with that exhibited,in the polymerization of the same monomer,by the systems CoCl_(2)(PR3)2-MAO and CoCl_(2)(PRPh2)2-MAO(R=alkyl or cycloalkyl group),and by the systems based on cobalt dichloride complexes with various bi-and tridentate ligands(e.g.,diphosphines,bis-imines,pyridyl-imines,bis-iminopyridines).The comparison between the different systems allowed us to make some clarity about the actual and effective role played by the various types of ligands in the polymerization of conjugated dienes with catalytic systems CoCl_(2)(L)-MAO,in which L=mono-,bi-,or tri-dentate ligand.

关键词： 1,3-Butadiene Stereospecific polymerization Cobalt catalysts Regioselectivity Aminophosphine ligands Crystal structures Chelate rings

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Methyltransferase-like 3(METTL3)inhibition potentiates anti-tumor immunity:a novel strategy for improving anti-PD1 therapy

引用

Signal Transduction and Targeted Therapy 2024年第1期9卷 10-11页

作者： anna Brichkina Roman Suezov Magdalena Huber Institute of Systems Immunology Member of the German Center for Lung Research(DZL)Philipps University of Marburg35043 MarburgGermany

In a recent article published in Cancer Discovery,Guirguis et *** that enzymatic inhibitors of the m6 A methyltransferase METTL3 induced a formation of immunostimulatory doublestranded mRNAs(dsRNAs)in cancer cells,which triggered a profound tumor-cell-intrinsic interferon response leading to an enhanced antigen presentation and thereby increased killing of tumor cells by cytotoxic CD8+*** inhibition of METTL3 synergized with anti-PD1 immunotherapy,highlighting its potential for improving cancer treatment.

关键词： PD1 immunity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biodegradability of bioplastics in different aquatic environments:A systematic review

引用

Journal of Environmental Sciences 2024年第8期142卷 169-181页

作者： Maria Cristina Lavagnolo Valentina Poli anna Maria Zampini Valentina Grossule Department of Civil Environmental and Architectural Engineering-Laboratory of Environmental EngineeringUniversity of PadovaLungargine Rovetta 8Padova 35100Italy

Bioplastics were first introduced as environmentally friendly materials,with properties similar to those of conventional plastics.A bioplastic is defined as biodegradable if it can be decomposed into carbon dioxide under aerobic degradation,or methane and CO_(2)under anaerobic conditions,inorganic compounds,and new cellular biomass,by the action of naturally occurring *** definition however does not provide any information on the environmental conditions,timescale and extent at which decomposition processes should *** regard to the aquatic environment,recognized standards have been established to assess the ability of plastics to undergo biodegradation;however,these standards fail to provide clear targets to be met to allow labelling of a bioplastic as ***,these standards grant the user an extensive leeway in the choice of process *** these reasons,the comparison of results deriving from different studies is *** authors analysed and discussed the degree of biodegradability of a series of biodegradable bioplastics in aquatic environments(both fresh and salt water)using the results obtained in the laboratory and from on-site testing in the context of different research *** Oxygen Demand(BOD),CO_(2)evolution,surface erosion and weight loss were the main parameters used by researchers to describe the percentage of *** results showed a large variability both in weight loss and BOD,even when evaluating the same type of *** confirms the need for a reference range of values to be established with regard to parameters applied in defining the biodegradability of bioplastics.

关键词： Biodegradation Bioplastic Plastic Natural environment Plastic waste

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：