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Emergence of Conilon Coffee Seedlings Originating from Seeds Treated with a Sodium Hypochlorite Solution

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American Journal of Plant Sciences 2014年第13期5卷 1819-1830页

作者： Raquel Fialho Rubim Henrique Duarte Vieira Eduardo Fontes Araújo anna christina sanazário de oliveira Alexandre Pio Viana Center of Agricultural Sciences and Technologies State University of North Fluminense Darcy RibeiroCampos dos GoytacazesBrazil department of Plant Science Federal University of VicosaVicosaBrazil

The coffee seeds are problematic for the physiologycal quality, featuring low speed of seedling emergence. In this sense, this work was developed with the objective of evaluating the effect of sodium hypochlorite in the removal of the parchment and in the emergence and seedlings development of conilon coffee in nursery conditions. Coffee seeds of the variety Victoria were used, which were harvested in the cherry stage and pulped by hand. Seeds were dried in an oven with forced ventilation until reaching moisture contents of 35%, 30% and 25% wet basis. Then, the seeds with parchment were soaked in a sodium hypochlorite solution with concentrations of 4%, 5%, 6% and 7% active chlorine for 3 and 6 hours. For each moisture content, two additional treatments were added consisting of seeds with parchment and seeds in which the structure was removed manually. The characteristics evaluated were: emergence speed index, total emergence, dry mass of the shoots and root system. Utilization of sodium hypochlorite promoted an acceleration in seedling emergence speed similar to the method for manual removal of the parchment when using concentrations of 4%, 5%, 6% and 7% for 3 hours, at all moisture contents.

关键词： Coffea canephora Soaking Seeds Parchment

Physiological Quality and Seed Production of Corn and Fabaceae in Monoculture and Intercropping

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American Journal of Plant Sciences 2017年第11期8卷 2597-2607页

作者： anna christina sanazário de oliveira Fábio Cunha Coelho Henrique Duarte Vieira Jocarla Ambrosim Crevelari Aurea Izabel Aguiar Fonseca e Souza Tiago Massi Ferraz Antonia Alice Costa Rodrigues Programa de Pós-Graduacao em Agroecologia Centro de Ciências AgráriasUniversidade Estadual do MaranhaoSao LuísBrasil Laboratório de Fitotecnia Campos dos GoytacazesUniversidade do Norte Fluminense Darcy RibeiroCamposBrasil Laboratório de Melhoramento Genético Vegetal Campos dos GoytacazesUniversidade do Norte Fluminense Darcy RibeiroCamposBrasil Universidade Federal Rural da Amazonia BelémBrasil

The aim of this study was to evaluate the physiological quality and seed production of Fabaceae and corn in monoculture and intercropping in areas cropped under different management. The following treatments were evaluated: corn + NPK + weed control;corn + weed control;corn + NPK;corn + crotalaria;corn + jack bean. Intercropping raised the average number of corn ears of corn and the seed IVG Weed control positively influenced the final stand and average number of ears. NPK fertilization on corn sowing positively influenced the seed electrical conductivity. The yield of corn plants intercropped with crotalaria and jack bean was statistically similar. However, their absolute values should be considered for economic purposes. Corn seeds derived from intercropping with crotalaria were more effective compared with intercropping with jack bean. Crotalaria and jack bean seeds when intercropped with corn have high production, germination and vigor (IVG and emergence).

关键词： Zea mays Green Manure No-Till System Vigor Germination

Effectiveness of BNT162b2 and CoronaVac vaccines against omicron in children aged 5 to 11 years

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World Journal of Pediatrics 2023年第10期19卷 949-960页

作者： Eduardo A.oliveira Maria christina L.oliveira Ana Cristina Simões e Silva Enrico A.Colosimo Robert H.Mak Mariana A.Vasconcelos Ludmila R.Silva Daniella B.Martelli Clara C.Pinhati Hercílio Martelli-Júnior department of Pediatrics Health Sciences Postgraduate ProgramSchool of MedicineFederal University of Minas Gerais(UFMG)R.Engenheiro Amaro Lanari 389/501Belo HorizonteMG30130-100Brazil department of Statistics Federal University of Minas GeraisBelo HorizonteMGBrazil department of Pediatrics Rady Children’s HospitalUniversity of CaliforniaLa JollaSan DiegoCAUSA Health Science/Postgraduate Program in Nursing.School of Nursing Federal University of Minas Gerais(UFMG)Belo HorizonteMG30130-100Brazil Health Science/Primary Care Postgraduate Program State University of Montes Claros(Unimontes)Montes ClarosMG39401-089Brazil

Background This study aimed to estimate vaccine effectiveness(VE)against omicron variant infection and severe corona virus disease 2019(COVID-19)in children aged 5–11 years hospitalized with acute respiratory *** A test-negative,case–control analysis was conducted from February 2022 to June *** enrolled 6950 eligible children,including 1102 cases and 5848 *** was calculated after immunization with one and two doses of BNT162b2 or *** outcomes were hospitalization with acute respiratory symptoms and detection of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection and severe *** adjusted odds ratio for the association of prior vaccination and outcomes was used to estimate *** For fully vaccinated children,the overall estimated VE against hospitalization with SARS-CoV-2 infection was 42%[95%confidence interval(CI)26 to 54].VE peaked at 29–42 days(67%,95%CI 40%to 82%)and then declined to 19%(95%CI,−20%to 45%)at 57–120 days after the second *** BNT162b2 vaccine had a similar VE against hospitalization with SARS-CoV-2 infection(45%,95%CI,20 to 61)compared to the CoronaVac vaccine(40%,95%CI,17%to 56%).Among cases,56(5%)children died;53(94.6%)were not fully *** cases,the two-dose schedule effectiveness against ICU admission,need for invasive ventilation,severe illness,and death were 10%(95%CI,−54%–45%),22%(95%CI−70%–68%),12%(95%CI,−62%–52%),and 16%(95%CI,−77%–75%),*** For hospitalized children aged 5–11 years during the omicron-predominant period in Brazil,two doses of both vaccines had moderate effectiveness against hospitalization with acute respiratory symptoms and SARS-CoV-2 infection and offered limited protection against endpoints of COVID-19 severity.

关键词： Children COVID-19 Effectiveness Omicron Vaccine

Ultrastructural Analysis of Chitosan Antibacterial Activity against Clinical Isolates of <i>Staphylococcus aureus</i>and <i>Escherichia coli</i>

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Advances in Microbiology 2019年第10期9卷 893-903页

作者： Maria Eduarda de Farias Albuquerque Gaspar de oliveira christina Alves Peixoto Rosa Valéria da Silva Amorim Programa de Pós-Graduação em Morfotecnologia Departamento de Histologia e Embriologia Universidade Federal da Pernambuco (UFPE) Recife Brazil departamento de Ultraestrutura Instituto Aggeu Magalhães/Fundação Oswaldo Cruz Recife Brazil

The continuous search for an antimicrobial agent led to the identification of potential antimicrobial biomaterials based on polymers naturals, such as chitosan (CS). However, the mechanism of action of antibacterial activity of CS for gram-positive and gram-negative bacteria was not completely elucidated. The aim of this work is to report the antibacterial activity of CS through ultrastructural analyses of the clinical isolates Staphylococcus aureus and Escherichia coli by Transmission Electron Microscopy. The CS has a bactericidal action against S. aureus and E. coli which alters its cellular ultrastructure, such as with collapsed cell walls, condensed chromatin and the increase of intracellulares structures like vacuoles and cell debris. In this way, the CS represents a potential model for the future design of antibacterial in order to control bacterial resistance of patients in hospital settings.

关键词： Antibacterial Activity Chitosan Ultrastructure Staphylococcus aureu Escherichia coli

determinant Factors in Maintaining the Exclusive Breastfeeding and Premature Weaning in Postpartum Mothers in the Brazilian Semiarid

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Health 2015年第3期7卷 355-364页

作者： Hellen Lívia oliveira Catunda Elizian Braga Rodrigues Bernardo Lara Leite de oliveira Mirna Fontenele de oliveira Régia christina Moura Barbosa Castro Priscila de Souza Aquino Ana Karina Bezerra Pinheiro department of Nursing Federal University of Ceará Fortaleza Brazil department of Nursing Federal University of Cariri Juazeiro do Norte Brazil

This is a cross-sectional, correlational and quantitative study conducted on 210 postpartum women from January to July 2013 at the breastfeeding outpatient clinic of a public maternity. The study was carried out in order to assess the determining factors in maintaining an exclusive breastfeeding in the first month of the newborn’s life, the participation of the nurse in encouraging this process, and the reasons leading to a premature weaning. Prior births proved to be a positive factor for exclusive breastfeeding (Odds Ratio (OR) 0.359;CI 95% 0.137 - 0.941;p = 0.031). The absence of a nurse in the medical appointments resulted in a threefold risk to have no prenatal exclusive breastfeeding guidance whatsoever (OR 3.025;CI 95% 1.567 - 5.840;p = 0.001). The reported reasons for a premature weaning were difficulty latching-on, cracked nipples, poor milk that does not satiate the baby’s hunger, and production of little or no milk at all. The results provide information for the adoption of strategies during pregnancy and the postpartum period aimed to promote an exclusive breastfeeding, checking for the possible influence of the woman’s personal circumstances in her choosing exclusive breastfeeding, as well as the participation of the nurse in the mother’s decision.

关键词： Breastfeeding Weaning Health Promotion Nursing

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Health 2014年第18期6卷 2375-2387页

作者： Cinthia Gondim Pereira Calou Ana Karina Bezerra Pinheiro Régia christina Moura Barbosa Castro Mirna Fontenele de oliveira Priscila de Souza Aquino Franz Janco Antezana departamento de Enfermagen Universidade Regional do Cariri Ceará Brasil department of Nursing Federal University of Ceará Fortaleza Brazil

Objective: The objectives were to characterize the scientific production on Health-Related Quality of Life of pregnant women, identify the areas that are most affected during pregnancy and puerperal period and identify the instruments used to assess quality of life related to health in pregnant women. Methods: For the integrative review, 11 articles published from 2006 to 2013 in the PUBMED, MEDLINE, CINAHL, SCOPUS and SCIELO were selected. Result: Data showed scarce publication from nursing professionals, prevalence of non-experimental studies conducted mainly in Brazil. The most commonly instruments used were the WHOQOL-BREF and SF-12. The presence of pain, nausea and vomiting, depression, low education, younger age and absence of partner negatively affect the quality of life of pregnant women. Practicing physical activity and being socially supported during pregnancy favour a better quality of life. In the domain of social relations, sexuality was the only affected facet. Conclusion: Gaps in the level of evidence considered weak were identified. We suggest bigger role of nurses in research on the subject so that there will be the development of effective interventions to support nursing practice and ensure quality care and consequently improve the quality of life of women in pregnancy and childbirth.

关键词： Prenatal Quality of Life Nursing

Maternal Predictors for Quality of Life during the Postpartum in Brazilian Mothers

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Health 2015年第3期7卷 371-380页

作者： Mirna Fontenele de oliveira Leslie Parker Hyochol Ahn Hellen Lívia oliveira Catunda Elizian Braga Rodrigues Bernardo Mara Fontenele de oliveira Samila Gomes Ribeiro Cinthia Gondim Pereira Calou Franz Janco Antezana Paulo César Almeida Régia christina Moura Barbosa Castro Priscila de Souza Aquino Ana Karina Bezerra Pinheiro College of Nursing University of Florida Gainesville USA department of Nursing Federal University of Ceará Fortaleza Brazil

Introduction: The postpartum period can have a significant physical, emotional, and social impact on the quality of a woman’s life. Most postpartum research has focused on physical complications and only a few studies have specifically investigated quality of life. The purpose of this study was to explore predictors affecting the quality of life of postpartum Brazilian mothers. Study design and Methods: A cross-sectional Quality of Life survey was performed in 210 Brazilian mothers during the early postpartum period. Data were collected using an interview technique and two instruments: 1) a maternal questionnaire and the 2) Maternal Postpartum Quality of Life tool/Brazilian version. The association between maternal characteristics and quality of life in the post-partum period was investigated with bivariate and multivariable analyses. Results: Mothers who had the best Quality of Life were white, registered students, 30 - 40 years of age, who were married or living with a partner, and without physical complaints;in addition, they had at least an 8th grade education, more than 4 children, and had attended more than 8 prenatal visits with a nurse. The stepwise model indicated that white race (p develop health interventions to enhance the quality of life of this population.

关键词： Mothers Postpartum Quality of Life Predictors

Knowledge of Female Prisoners in a City of Ceará, Brazil, about Sexually Transmitted Diseases

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Open Journal of Nursing 2014年第9期4卷 630-636页

作者： Cinthia Gondim Pereira Calou Camila Fonseca Wasidi Maria de Fátima Esmeraldo Ramos de Figueiredo Ana Izabel oliveira Nicolau Ana Karina Bezerra Pinheiro Régia christina Moura Barbosa Castro Priscila de Souza Aquino Universidade Federal do Ceará Fortaleza Brazil Universidade Federal do Ceará Fortaleza Brazil Universidade Regional do Cariri Crato Brazil Universidade Regional do Cariri Crato Brazil

Exploratory study was conducted in the Crato Public Prison, State of Ceará, Brazil, aiming to understand the knowledge and experiences related to the preventive aspects of Sexually Transmitted Diseases (STDs) in the prison context. The target population of the study consisted of 14 female inmates, 10 of whom agreed to participate. Data collection occurred in June 2012 through recorded semi-structured individual interviews. We investigated quantitative, socio-demographic and sexual variables. In qualitative data analysis, we applied the Meaning Interpretation Method. The female vulnerability was aggravated by the early onset of sexual activity, lack of knowledge about STDs, non-adoption of preventive measures and history of prostitution. The following categories emerged from the qualitative assessment: Knowledge about STD/AIDS versus Personal Experience;Use of contraception as STD/AIDS prevention within the Public Jail;Habits of inmates: alcohol and tobacco;and Characterization of the conjugal visit area.

关键词： Prisoners Nursing Women’s Health Sexual and Reproductive Health

Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases

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World Journal of Gastroenterology 2006年第41期12卷 6627-6633页

作者： Claudia Rubie Vilma oliveira Frick Mathias Wagner christina Weber Bianca Kruse Katja Kempf Jochen Knig Bettina Rau Martin Schilling department of General- Visceral-Vascular-and Paediatric SurgeryUniversity of the SaarlandHomburg/Saar 66421Germany Institute of Pathology University of the SaarlandHomburg/Saar 66421Germany Institute of Medical Biometrics Epidemiologyand Medical InformaticsUniversity of the SaarlandHomburg/ Saar 66421Germany

AIM: To evaluate and compare the expression profiles of CXCL12 (SDF-1), CCL19 (MIP-3β), CCL20 (MIP-3α) and CCL21 (6Ckine, Exodus2) and their receptors on RNA and protein levels in hepatocellular carcinoma (HCC) versus colorectal liver metastases (CRLM) and to elucidate their impact on the carcinogenesis and progression of malignant liver diseases. METHODS: Chemokine expression was analyzed by RT-PCR and ELISA in 11 cases of HCC specimens and in 23 cases of CRLM and corresponding adjacent non- tumorous liver tissues, respectively. Expressions of their receptors CXCR4, CCR6 and CCR7 were analyzed by RT- PCR and Western blot analysis in the same cases of HCC and CRLM. RESULTS: Significant up-regulation for CCL20/CCR6 was detected in both cancer types. Moreover, CCL20 demonstrated significant overexpression in CRLM in relation to the HCC tissues. Being significantly up- regulated only in CRLM, CXCR4 displayed an aberrant expression pattern with respect to the HCC tissues. CONCLUSION: Correlation of CXCR4 expression with CRLM suggests CXCR4 as a potential predictive factor for CRLM. High level expression of CCL20 and its receptor CCR6 in HCC and CRLM with marked up- regulation of CCL20 in CRLM in relation to HCC tissues indicates involvement of the CCL20/CCR6 ligand-receptor pair in the carcinogenesis and progression of hepatic malignancies.

关键词：肝细胞癌基因表达肿瘤转移病理