检索结果-南通市图书馆

Detection of ca^(2+)-dependent acid phosphatase activity identifies neuronal integrity in damaged rat central nervous system after application of bacterial melanin

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第7期11卷 1147-1152页

作者： Tigran R. Petrosyan anna s. Ter-Markosyan anna s. Hovsepyan Yerevan state Medical University YerevanArmenia sPc"Armbiotechnology"sNPO NAs RA YerevanArmenia

The study aims to confirm the neuroregenerative effects of bacterial melanin （BM） on central nervous system injury using a special staining method based on the detection of ca^2＋-dependent acid phosphatase activity. Twenty-four rats were randomly assigned to undergo either unilateral destruction of sensorimotor cortex （group I; n = 12） or unilateral rubrospinal tract transection at the cervical level （c3-4）（group II; n = 12）. In each group, six rats were randomly selected after surgery to undergo intramuscular injection of BM solution （BM subgroup） and the remaining six rats were intramuscularly in）ected with saline （saline subgroup）. Neurological testing confirmed that BM accelerated the recovery of motor function in rats from both BM and saline subgroups. Two months after surgery, ca^2＋-dependent acid phosphatase activity detection in combination with chilingarian＇s calcium adenoside triphosphate method revealed that BM stimulated the sprouting of fibers and dilated the capillaries in the brain and spinal cord. These results suggest that BM can promote the recovery of motor function of rats with central nervous system injury; and detection of ca^2＋-dependent acid phosphatase activity is a fast and easy method used to study the regeneration-promoting effects of BM on the injured central nervous system.

关键词： nerve regeneration bacterial melanin histochemical analysis rubrospinal tract sensorimotor cortex ca^2+-dependent acid phosphatase activity rats neural regeneration

When Kinases Meet PROTAcs

在线全文

学校读者我要写书评

暂无评论

chinese Journal of chemistry 2018年第10期36卷 971-977页

作者： Li tan Nathanael s. Gray Interdisciplinary Research center on Biology and chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences 26 Qiuyue Rd Pudong Shanghai 201210 China Department of cancer Biology Dana-Father Cancer Institute Boston MA 02215 USA Department of Biochemistry and Molecular Pharmacology Harvard Med- ical School Boston MA 02115 USA

small molecule drugs targeting kinases have revolutionized treatment options for millions of patients worldwide, especially in oncology. These targeted treatments have less side effects because they inhibit a specific dysfunctional kinase usually with relatively narrow selectivity. However, kinase inhibitors do have well-established liabilities, most prominently the emergence of drug resistance. Moreover, the majority of kinases are multi- domain and multifunctional proteins that in addition to their enzymatic activity have scaffolding and other roles, and inhibitors seldom address these alternative functions. Recently, small molecule mediated targeted protein degradation emerged as a new pharmacological strategy. The majority of small molecule degraders are bispecific molecules called proteolysis targeting chimeras （PROTAcs）, and their mechanism of action is based on simultaneous recruitment of the target of interest and an E3 ligase, resulting in target polyubiquitination and eventual destruction by the proteasome. Over the last couple of years, PROTAc strategy has been developed and validated for a range of targets, including kinases. Here, we introduce the targeted protein degradation strategy, provide an overview of representative kinase PROTAcs, and describe design rationales, efficacy and specificity. We also discuss their potential advantages, as well as comment on some of the limitations of this emerging pharmacological modality.

关键词： kinase inhibitors PROTAcs drug discovery ligases kinase degradation drug design

Deciphering the mechanobiology of microglia in traumatic brain injury with advanced microsystems

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2304-2306页

作者： Anthony Procès sylvain Gabriele Univ.Grenoble Alpes InsermU1216Grenoble Institut des NeurosciencesGrenobleFrance Mechanobiology&Biomaterials Group CIRMAPResearch Institute for BiosciencesUniversity of MonsMonsBelgium

Advanced microsystems in traumatic brain injury research:Traumatic brain injury(TBI)results from a mechanical insult to the brain,leading to neuronal and axonal damage and subsequently causing a secondary *** minutes ... 详细信息

关键词： traumatic injury damage

计算机视觉技术赋能大学英语课堂展示中的学习投入研究

在线全文

学校读者我要写书评

暂无评论

新疆师范大学学报（哲学社会科学版） 2025年第2期46卷 144-151页

作者：谭苏燕王晋军广州大学外国语学院广东广州510010

学习投入是预测学习者学习效果的重要指标,学术界对其与课堂展示间的相关性研究鲜有涉猎。文本以某综合性大学303名非英语专业本科生为研究对象,采用人工智能与计算机视觉技术模型YOLOv7对大学英语课堂中的课堂展示实录进行学习投入多... 详细信息

学习投入是预测学习者学习效果的重要指标,学术界对其与课堂展示间的相关性研究鲜有涉猎。文本以某综合性大学303名非英语专业本科生为研究对象,采用人工智能与计算机视觉技术模型YOLOv7对大学英语课堂中的课堂展示实录进行学习投入多模态数据分析,并辅以问卷调查,形成双向印证。研究发现,学生课堂展示中的行为投入、认知投入和情感投入值均等于或高于3.78,投入结果均值达4.01,说明学生在课堂展示中的学习投入情况良好。此外,不同时段课堂展示对学生行为投入的影响显著,学生专注度及参与度在课堂前10分钟的课堂展示中效果最佳。文本为课堂展示的有效性提供了智能化、多模态、多维度的实证支持,为课堂教学的数智技术应用提供了新视角。

关键词：大学英语课堂展示学习投入计算机视觉技术展示时段

Emerging structures and dynamic mechanisms ofγ-secretase for Alzheimer’s disease

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 174-180页

作者： Yinglong Miao Michael s.Wolfe computational Medicine Program and Department of Pharmacology University of North Carolina–Chapel HillChapel HillNCUSA Department of Medicinal chemistry University of KansasLawrenceKSUSA

γ-secretase,called“the proteasome of the membrane,”is a membrane-embedded protease complex that cleaves 150+peptide substrates with central roles in biology and medicine,including amyloid precursor protein and the Notch family of cell-surface *** inγ-secretase and amyloid precursor protein lead to early-onset familial Alzheimer’s disease.γ-secretase has thus served as a critical drug target for treating familial Alzheimer’s disease and the more common late-onset Alzheimer’s disease as ***,critical gaps remain in understanding the mechanisms of processive proteolysis of substrates,the effects of familial Alzheimer’s disease mutations,and allosteric modulation of substrate cleavage byγ-*** this review,we focus on recent studies of structural dynamic mechanisms ofγ-*** mechanisms,including the“Fit-stay-Trim,”“sliding-Unwinding,”and“Tilting-Unwinding,”have been proposed for substrate proteolysis of amyloid precursor protein byγ-secretase based on all-atom molecular dynamics *** an incorrect registry of the Notch1 substrate was identified in the cryo-electron microscopy structure of Notch1-boundγ-secretase,molecular dynamics simulations on a resolved model of Notch1-boundγ-secretase that was reconstructed using the amyloid precursor protein-boundγ-secretase as a template successfully capturedγ-secretase activation for proper cleavages of both wildtype and mutant Notch,being consistent with biochemical experimental *** approach could be potentially applied to decipher the processing mechanisms of various substrates byγ-*** addition,controversy over the effects of familial Alzheimer’s disease mutations,particularly the issue of whether they stabilize or destabilizeγ-secretase-substrate complexes,is ***,an outlook is provided for future studies ofγ-secretase,including pathways of substrate binding and product release,effects of modulators on familial Alzheimer’s disease mutations

关键词： Alzheimer’s disease amyloid precursor protein cryo-EM structures drug design intramembrane proteolysis molecular dynamics Notch

Perception of artificial conspecifics by bearded dragons(Pogona vitticeps)

在线全文

学校读者我要写书评

暂无评论

Integrative Zoology 2019年第2期14卷 214-222页

作者： anna FROHNWIEsER Thomas W.PIKE John c.MURRAY anna WILKINsON school of Life sciences University of LincolnLincolnUK school of computer science University of LincolnLincolnUK Wildlife Research center Kyoto UniversityKyotoJapan

Artificial animals are increasingly used as conspecific stimuli in animal behavior ***,researchers often have an incomplete understanding of how the species under study perceives conspecifics,and,hence,which features are needed for a stimulus to be perceived *** investigate the features that bearded dragons(Pogona vitticeps)attend to,we measured their lateralized eye use when assessing a successive range of *** ranged through several stages of realism in artificial conspecifics,to see how features such as color,the presence of eyes,body shape and motion influence *** found differences in lateralized eye use depending on the sex of the observing bearded dragon and the artificial conspecific,as well as the artificial conspecific’s ***,this approach can inform the design of robotic animals that elicit biologically-meaningful responses in live animals.

关键词： bearded dragons conspecifics lateralized eye use perception

The andropause and memory loss: is there a link between androgen decline and dementia in the aging male?

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2001年第3期3卷 169-174页

作者： Robert s.tan shou-Jin Pu Geriatric Medicine Fellowship Program University of Texas Houston United StatesGeriatric Medicine Fellowship Program University of Texas Houston United States

studies demonstrate a decline in androgens with age and this results in the andropause. The objective of this paperis to review the literature on hormonal changes that occur in the aging males and determine if there are associations be-tween decreased testosterone, dehydroepiandrosterone (DHEA) and decreased cognitive function. Trials of androgen re-placement and its impact on cognitive function will also be analyzed. Method of analysis will be by a thorough searchof articles on MEDLINE, the Internet and major abstract databases. Results of the author's own research in 302 men ofthe association of memory loss as a symptom in the andropause will be presented. In addition, the authors open trial oftestosterone replacement in hypogonadic men with Alzheimer's disease will also be presented. The results of the author'strial will be compared with other investigators. High endogenous testosterone level predicted better performance on visu-al spatial tests in several studies, but not in all studies. Likewise, testosterone replacement in hypogonadic patients im-proved cognitive functions in some but not all studies. Testosterone has also been shown to improve cognitive functionin eugonadal men. several studies have shown that declines in DHEA may contribute to Alzheimer's disease and the re-sults of double blind studies with DHEA replacement and its effect on cognition will also be presented. In summary,there is still no consensus that androgen replacement is beneficial in cognitive decline but this option may prove promis-ing in some patients. (Asian J Androl 2001 sep; 3: 169-174)

关键词： andropause memory loss androgens testosterone aging dementia

Order Exponential Evaluation Model for Road Traffic safety in city clusters

在线全文

学校读者我要写书评

暂无评论

Journal of Harbin Institute of Technology(New series) 2021年第1期28卷 53-61页

作者： Qizhou Hu s.c.Wong Y.c.Li Minjia tan school of Automation Nanjing University of Science and TechnologyNanjing 210094China Department of civil Engineering The University of Hong KongHong Kong 999077China

This study presents an order exponential model for estimating road traffic safety in city *** proposed model introduces the traffic flow intrinsic properties and uses the characteristics and regular patterns of traffic development to identify road traffic safety levels in city ***,an evaluation index system of city cluster road traffic safety was constructed based on the spatial and temporal *** Order Exponential Evaluation Model(OEEM),a comprehensive model using order exponent function for road traffic safety evaluation,was put forward,which considers the main characteristics and the generation process of traffic *** model effectively controlled the unsafe behavior of the traffic *** could define the levels of city cluster road traffic safety and dynamically detect road safety *** proposed model was verified with statistical data from three chinese city clusters by comparing the common model for road traffic safety with an ideal *** results indicate that the order exponent approach undertaken in this study can be extended and applied to other research topics and fields.

关键词： city cluster road traffic safety evaluation model order exponential function

Multiple sclerosis is at a checkpoint: advancing the program

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第3期20卷 811-812页

作者： Brandon c.smith Jessica L.Williams Department of Neurosciences Lerner Research InstituteCleveland ClinicClevelandOHUSA Department of Biological Geologicaland Environmental SciencesCleveland State UniversityClevelandOHUSA

Multiple sclerosis(Ms) is a chronic inflammatory and demyelinating disease of the central nervous system(cNs). Patients with Ms experience sensory and motor function loss due to myelin and/or axon damage perpetuated b... 详细信息

关键词： Hauser sclerosis damage