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High‐throughput formation of miniaturized cocultures of 2D cell monolayers and 3D cell spheroids using droplet microarray

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Droplet 2023年第1期2卷 36-46页

作者： Haijun Cui Tina Tronser Xianxian Wang Janine Wesslowski Gary Davidson anna a.popova Pavel A.Levkin Institute of Biological and Chemical Systems‐Functional Molecular Systems(IBCS‐FMS) Karlsruhe Institute of Technology(KIT)Eggenstein‐LeopoldshafenGermany Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqingChina Institute of Organic Chemistry Karlsruhe Institute of Technology(KIT)KarlsruheGermany

Most of the biological processes,including cell signaling,cancer invasion,embryogenesis,or neural development,are dependent on and guided by the complex architecture and composition of cellular *** such microenvironments in cell coculture models is crucial for fundamental and applied biology *** ability to combine different cell types grown as both two‐dimensional(2D)monolayers and three‐dimensional(3D)spheroids in specific defined location inside a microculture environments is a key towards in vitro tissue modeling and towards mimicking complex in vivo cellular *** this study,we introduce and investigate a method to create in vitro models of 2D cell monolayers cocultured with 3D spheroids in defined *** demonstrate the possibility of creating such complex cellular microenvironments in a high‐throughput and automated manner by creating arrays of such droplets containing prearranged 2D and 3D cellular ***,we demonstrate an application of this approach to study paracrine propagation of Wnt signaling between 2D and 3D cellular *** method provides a general approach for the miniaturized,high‐throughput,and automated formation of complex coculture cellular microarchitectures that will be useful for mimicking various in vivo complex cellular structures and for studying complex biological processes in vitro.

关键词： invasion architectures coculture

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Digital twin of a droplet microarray platform:Evaporation behavior for multiple droplets on patterned chips for cell culture

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Droplet 2024年第1期3卷 113-124页

作者： Yanchen Wu Joaquin E.Urrutia Gomez Hongmin Zhang Fei Wang Pavel A.Levkin anna a.popova Britta Nestler Institute for Applied Materials–Microstructure Modeling and Simulation Karlsruhe Institute of TechnologyKarlsruheGermany Institute of Nanotechnology Karlsruhe Institute of TechnologyEggenstein‐LeopoldshafenGermany Institute of Biological and Chemical Systems(IBCS‐FMS) Karlsruhe Institute of TechnologyEggenstein‐LeopoldshafenGermany Institute of Organic Chemistry Karlsruhe Institute of TechnologyKarlsruheGermany Institute of Digital Materials Science Karlsruhe University of Applied SciencesKarlsruheGermany

Precise control of the evaporation of multiple droplets on patterned surfaces is crucial in many technological applications,such as anti‐icing,coating,and high‐throughput ***,the complex evaporation process of multiple droplets on well‐defined patterned surfaces is still poorly ***,we develop a digital twin system for real‐time monitoring of key processes on a droplet microarray(DMA),which is essential for parallelization and automation of the operations for cell ***,we investigate the evaporation of multiple nanoliter droplets under different conditions via experiments and numerical *** demonstrate that the evaporation rate is not only affected by the environmental humidity and temperature but is also strongly linked to the droplet distribution on the patterned surfaces,being significantly reduced when the droplets are densely ***,we propose a theoretical method to aid in the experimental detection of volumes and pH variation of evaporating droplets on patterned *** versatile and practical strategy allows us to achieve active maneuvering of the collective evaporation of droplets on a DMA,which provides essential implications for a wide range of applications including cell culture,heat management,microreactors,biochips,and so on.

关键词： coating culture collective

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Fine structure and phase composition of Fe–14Mn–1.2C steel:influence of a modified mixture based on refractory metals

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International Journal of Minerals,Metallurgy and Materials 2017年第5期24卷 523-529页

作者： anna Zykova Natalya.popova Mark Kalashnikov Irina Kurzina Tomsk Polytechnic University Tomsk State University of Architecture and Building Chemical Department Tomsk State University

The effect of TiO;,ZrO;and Na;AlF;ultrafine powders on the fine structure and the phase composition of Fe–14Mn–1.2C steel was *** introduction of the ultrafine powders into the melt influenced the grain size,the quantity,and the character of distribution of nonmetallic inclusions in the railroad *** microstructure of castings was improved significantly because of the refinement of the grain structure and an increase of the grain-boundary *** the modifying mixture was introduced into the melt,either the microtwins of one or two intersecting systems or the precipitations of ε-martensite of different types,or simultaneously the microtwins and wafers of ε-martensite,were present in each grain.

关键词： manganese steel fine structure phase composition refractory metals

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Early Miocene flora of central Kazakhstan(Turgai Plateau) and its paleoenvironmental implications

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Plant Diversity 2019年第3期41卷 183-197页

作者： popova Svetlana Utescher Torsten Averyanova anna Tarasevich Valentina Tropina Polina Xing Yaowu Komarov Botanical Institute of RAS Palaeobotanical Department 2 Professor Popov Str. Saint Petersburg 197376 Russia Steinmann Institute Bonn University Nussallee 8 Bonn D-53115 Germany Senckenberg Research Institute and Natural History Museum/Biodiversity and Climate Research Centre Senckenberganlage 25 Frankfurt D-60325 Germany Xishuangbanna Tropical Botanical Garden of Chinese Academy of Sciences (CAS) Menglum Mengla Yunnan 666303 China

The investigation of the fossil floras from the Turgai plateau (central Kazakhstan) contributes to a better understanding of the origin of the temperate Turgai type flora which spread to Kazakhstan and adjacent areas during the Oligocene-Miocene transition. In this paper, we present the results of a carpological and palynological study of the Kumyrtas flora collected from a flora-bearing horizon of the regional coalbearing Zhilanchik suite, dated to the Aquitanian period. Pollen analysis identified 33 taxa, with are dominated by angiosperms (about 73%) and reflect zonal vegetation. The high percentages of Betula (27%) and Pterocarya (7.5%) that were found in this flora allows comparisons with other Aquitanian floras of Kazakhstan. Based on descriptions of fossil fruits and seeds, we determined that 19 taxa were dominant;these taxa had meosphytic herbaceous components, suggesting mostly edaphic local conditions. The incongruence between the carpological and the pollen records suggests a significant taphonomical effect. Quantitative reconstruction of the palaeoclimate based on pollen records supports—and slightly extends—previous findings based on fossil leaf data, but contradicts findings deduced from the carpological record. Plant Functional Type (PFT) classification was used to characterize the vegetation patterns. Pollen records show that about 45% of diversity relates to the arboreal broadleaved deciduous components and ca. 35% to conifers. Fossil fruit and seed data indicate riverine vegetation with a high diversity of aquatic components and shrub stratum.

关键词： Kazakhstan Turgai flora Oligocenee-Miocene transition Palaeoclimate reconstruction PFT

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High-resolution Fourier spectroscopy as a tool for studying quality of rare-earth-doped crystals

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Journal of Rare Earths 2014年第3期32卷 230-235页

作者： Marina N.popova Institute of Spectroscopy Russian Academy of Sciences 5 Fizicheskaya Str. 142190 Troitsk Moscow Russia

Three examples were considered of the use of high resolution Fourier-transform optical spectroscopy for studying quality of rare-earth-doped crystals. The first example was connected with defects present in crystals grown by flux techniques. The second example dealt with detection of stresses and deformations in rare-earth-containing crystals, by registering splitting of spectral lines. The third example showed that a very small amount （at the level ofppm） of different RE ions present in a crystal could be determined using high-resolution spectroscopy. This work was carried out by the author＇s group in collaboration with several institutions in Rus- sia and abroad.

关键词： Fourier-transform optical spectroscopy high resolution absorption spectra rare earths

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Spectroscopy of compounds from the family of rare-earth orthoborates

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Journal of Rare Earths 2009年第4期27卷 607-611页

作者： Marina N.popova Institute of Spectroscopy Russian Academy of Sciences 5 Fizicheskaya Str. Troitsk Moscow Region 142190 Russia

This paper is devoted to the rare-earth borates described by the general formula RM3（BO3）4, M = Al, Fe, and Cr, that have non-centrosymmetric trigonal slructure of the natural mineral huntite. It give a brief review of a spectroscopic research on YbAI3（BO3）4 （YbAB）, on a large group of magnetic iron borates, and on NdCra（BO3）4.

关键词： YbAl3(BO3)4 rare-earth iron and chromium borates magnetic ordering Fourier-transform optical spectroscopy

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On the metallicity gradient in the Galactic disk

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Research in Astronomy and Astrophysics 2020年第4期20卷 123-128页

作者： Alexander Loktin Maria.popova Astronomical Observatory Ural Federal UniversityEkaterinburgRussia

The problem of the chemical composition gradient in the Galactic disk is studied based on a sample of metallicity estimates of open star clusters,using Gaia DR2-improved distance estimates.A clearly non-monotonic variation was observed in the average metallicity of clusters with increasing Galactocentric *** can clearly see the metallicity jump of 0.22 in[Fe/H]at a Galactocentric distance of about9.5 kpc,which appears to be linked to the outer boundary of the thinnest and youngest component of the Galactic *** absence of a significant metallicity gradient in the internal(R10 kpc)regions of the disk demonstrates the absence of noticeable metal enrichment at times of the order of the ages corresponding to those of the disk regions under *** data show that the disk experiences noticeable metal enrichment only during the starburst *** significant dependence was identified between the average metallicity and the age of the clusters.

关键词： Galaxy:general Galaxy:disk (Galaxy:)open clusters and associations:general

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Timoshenko inclusions in elastic bodies crossing an external boundary at zero angle

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Acta Mechanica Solida Sinica 2017年第3期30卷 327-333页

作者： A.M. Khludnev T.S. popova Lavrentyev Institute of Hydrodynamics of R_AS Novosibirsk State University Novosibirsk 630090 Russia North-Eastern Federal University Yakutsk 677000 Russia

The paper concerns an analysis of equilibrium problems for 2D elastic bodies with a thin Timoshenko inclusion crossing an external boundary at zero angle. The inclusion is assumed to be delaminated, thus forming a crack between the inclusion and the body. We consider elastic inclusions as well as rigid inclusions. To prevent a mutual penetration between the crack faces, inequality type boundary conditions are imposed at the crack faces. Theorems of existence and uniqueness are established. Passages to limits are investigated as a rigidity parameter of the elastic inclusion going to infinity.

关键词： Thin Timoshenko inclusion Crack Delamination Fictitious domain method Non-penetration

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The research works of Coulomb and Amontons and generalized laws of friction

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Friction 2015年第2期3卷 183-190页

作者： Elena.popova Valentin L.POPOV Institute of Mechanics Technische Universität Berlin10623Berlin National Research Tomsk State University 634050 TomskRussia

The paper is devoted to the contributions of Coulomb and Amontons to the physics of friction from the viewpoint of current discussions and attempts to formulate generalized laws of friction.

关键词： static friction sliding friction kinetics of friction material dependency history of science

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Understanding of Visual Sociology as an Independent Discipline

Understanding of Visual Sociology as an Independent Discipli...

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作者： Anna.popova National Research University Bauman Moscow State Technical University (BMSTU)

In this paper, the stages of the visual sociology development as an independent discipline in the structure of scientific knowledge are shown and the core elements of this approach to the study of the social processes are defined. The purpose of the article is to substantiate the place of visual sociology as an independent scientific discipline in sociological knowledge. Evaluation of the methods used in visual sociology and their relevance in connection with the growth and development of new technologies that form a new virtual visual human environment. The inclusion of visual sociology in the BMSTU curricula for the sociologists training is substantiated.

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