检索结果-南通市图书馆

Defining the‘HoneySweet’insertion event utilizing NextGen sequencing and a de novo genome assembly of plum(Prunus domestica)

引用

Horticulture Research 2021年第1期8卷 25-37页

作者： ann M.Callahan Tetyana N.Zhebentyayeva Jodi l.Humann Christopher A.Saski Kelsey D.Galimba laura l.Georgi Ralph Scorza Dorrie Main Christopher D.Dardick USDA-ARS Appalachian Fruit Research StationKearneysvilleWV 25430USA The Schatz Center for Tree Molecular Genetics Department of Ecosystem Science and ManagementThe Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Horticulture Washington State UniversityPullmanWA 99164USA Plant and Environmental Sciences Department Clemson UniversityClemsonSC 29634USA Department of Genetics and Biochemistry Clemson UniversityClemsonSC 29634USA

HoneySweet’plum(Prunus domestica)is resistant to Plum pox potyvirus,through an RNAi-triggered *** the precise nature of the transgene insertion event has been complicated due to the hexaploid genome of *** blots previously indicated an unintended hairpin arrangement of the Plum pox potyvirus coat protein gene as well as a multicopy insertion *** confirm the transgene arrangement of the insertion event,‘HoneySweet’DNA was subjected to whole genome sequencing using Illumina short-read *** indicated two different insertion events,one containing seven partial copies flanked by putative plum DNA sequence and a second with the predicted inverted repeat of the coat protein gene driven by a double 35S promoter on each side,flanked by plum *** determine the locations of the two transgene insertions,a phased plum genome assembly was developed from the commercial plum‘Improved French’.A subset of the scaffolds(2447)that were>10 kb in length and representing,>95%of the genome were annotated and used for alignment against the‘HoneySweet’transgene *** of eight matching scaffolds spanned both insertion sites ranging from 157,704 to 654,883 bp apart,however we were unable to identify which scaffold(s)represented the actual location of the insertion sites due to potential sequence differences between the two plum ***,there was no evidence of any gene(s)being interrupted as a result of the ***,RNA-seq data verified that the insertions created no new transcriptional units and no dramatic expression changes of neighboring genes.

关键词： plum insertion Sweet

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

活动期糖尿病夏科神经性骨关节病诊疗指南——《国际糖尿病足工作组:糖尿病相关的足病预防与管理指南(2023)》的一部分

引用

感染、炎症、修复 2024年第1期25卷 52-71页

作者： Dane K.Wukich Nicolaas C.Schaper Catherine Gooday Arun Bal Robert Bem Avneesh Chhabra Mary Hastings Crystal Holmes Nina l.Petrova Maria Gala Santini Araujo Eric Senneville Katherine M.Raspovic on behalf of the International Working Group on the Diabetic Foot 赵宏谋徐俊(译) 张明珠(审校) Department of Orthopaedic Surgery University of Texas Southwestern Medical CenterDallasTexasUSA Division of Endocrinology MUMC+CARIM and CAPHRI InstituteMaastrichtthe Netherlands Elsie Bertram Diabetes Centre Norfolk&Norwich University Hospitals NHS Foundation TrustNorfolkUK Secretary International Association of Diabetic Foot SurgeonsMumbaiIndia Diabetes Centre Institute for Clinical and Experimental MedicinePrague Department of Radiology UT Southwestern Medical CenterDallasTexasUSA Program in Physical Therapy Washington University School of MedicineSt.LouisMissouriUSA The Division of Metabolism Endocrinology and DiabetesThe University of Michigan Medical SchoolAnn ArborMichiganUSA Department of Diabetes Diabetic Foot ClinicKing’s College Hospital NHS Foundation TrustLondonUK Italian Hospital of Buenos Aires Buenos AiresArgentina Department of Infectious Diseases Gustave Dron HospitalTourcoingFrance 不详西安交通大学医学院附属红会医院陕西西安710054 天津医科大学朱宪彝纪念医院天津300314 首都医科大学附属同仁医院北京100730

国际糖尿病足工作组(IWGDF)自1999年以来发布了糖尿病足病预防和治疗的循证指南。这是IWGDF发布的第一部关于糖尿病患者活动期夏科神经性骨关节病诊断和治疗的指南。我们遵循GRADE方法,以人群-评估-比较-结局(PACO)和人群-干预-比较-结... 详细信息

国际糖尿病足工作组(IWGDF)自1999年以来发布了糖尿病足病预防和治疗的循证指南。这是IWGDF发布的第一部关于糖尿病患者活动期夏科神经性骨关节病诊断和治疗的指南。我们遵循GRADE方法,以人群-评估-比较-结局(PACO)和人群-干预-比较-结局(PICO)的格式设计临床问题,系统检索医学文献,并提出有依据的推荐。这些推荐是基于我们系统综述的证据,如无证据则参考专家意见,并考虑到与干预相关的受益和危害、患者偏好、可行性和适用性以及成本的权衡。我们在本章节介绍了2023年糖尿病患者活动期夏科神经性骨关节病的诊断和治疗指南,并提出了未来的关键研究方向。

关键词：糖尿病夏科神经性骨关节病,活动期诊断治疗国际糖尿病足工作组指南

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Single small molecule-assembled nanoparticles mediate efficient oral drug delivery

引用

Nano Research 2019年第10期12卷 2468-2476页

作者： Xin Yang Chao Ma Zeming Chen Jun liu Fuyao liu Rongbin Xie Haitian Zhao Gang Deng ann TChen Ningbo Gong lei Yao Pengjian Zuo Kangkang Zhi Jiacheng Wang Xiaobin Gao Jing Wang louzhen Fan Jiangbing Zhou School of Chemistry and Chemical Engineering Harbin Institute of TechnologyHarbin 150090China College of Biological Sciences and Biotechnology Beijing Forestry UniversityBeijing 100083China Department of Neurosurgery Yale UniversityNew HavenCT 06510USA Department of Chemistry Beijing Normal UniversityBeijing 100875China Department of Biomedical Engineering Yale UniversityNew HavenCT 06510USA Beijing Key laboratory of Polymorphic Drugs Institute of Materia MedicoChinese Academy cf Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Pathology Yale UniversityNew HavenCT 06510USA

Oral drug delivery,which requires surviving the harsh environment in the gastrointestinal(Gl)tract and penetrating the intestinal epithelium,has not bee n achieved using simple formulatio n nan oparticles(NPs).Medici nal natural products(MNPs)have bee n widely used in traditi onal medicine for disease management through oral ***,most pharmacologically active compounds within MNPs do not have the properties suitable for oral applicatio *** hypothesize that some MNPs contain n atural nano materials that can convert those compounds into oral formulations by forming *** screening 66 MNPs,we identified five classes of small molecules that form NPs,many of which are capable of efficient drug encapsulation and Gl *** show that one of them,dehydrotrametenolic acid(DTA),is capable of mediating oral delivery for effective disease *** determined that DTA NPs assemble through hydrogen bonding and penetra怕the Gl tract via apical sodium-depe ndent bile acid tran *** study reveals a no vel class of single comp orient,small molecule-assembled NPs for oral drug delivery,and suggests a n ovel approach to modernizi ng MNPs through nano material discovery.

关键词： natural material oral drug delivery nano particle dehydrotrametenolic acid(DTA)

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oral product input to the GI tract： GIS an oral product performance technology

引用

Frontiers of Chemical Science and Engineering 2017年第4期11卷 516-520页

作者： Gordon l. Amidon Yasuhiro Tsume College of Pharmacy University of Michigan Ann Arbor MI 48109 USA

The patient receives a pharmaceutical product, not a drug. The pharmaceutical products are formulated with a drug, an active ingredient to produce the maximum therapeutic effect after oral absorption. Therefore, it is the product we must optimize for the patients. In order to assure the safety and efficacy of pharmaceutical products, we need an in vivo predictive tool for oral product performance in patients. Currently, we are a surprisingly long way from accomplishing that objective. If the 20th century was the ＇age of the drug＇, i.e., the ＇magic bullet＇, the 21st century must become the ＇age of the guided missile＇, i.e., the delivery system, including the form of the active pharmaceutical ingredient （API）（＇drug＇）. The physical form of the drug and the delivery system must be optimized to maximize the therapeutic benefits of pharmaceutical products for humans. Oral immediate release （IR） dosage forms cannot be optimal for all drugs or likely even any drugs （APIs）. Still, the formulation of pharmaceutical products has to be optimized for patients. But how do we optimize oral delivery of drugs？ It is usually through ＇trial and error＇, in humans! We need a better way to optimize the oral dosage forms. We have suggested to select different dissolution methodologies for this optimization based on BCS Subclasses. In this article, we present the predicted in vivo drug dissolution profile of ketoconazole as a model drug from our laboratory utilizing a gastrointestinal simulator （GIS）, which is an adaptation of the ASD system. GIS consists of three chambers representing stomach, duodenum, and jejunum, to create the human gastrointestinal tract-like environment and enable the control the gastric emptying rate. This dissolution system allows the monitoring of the drug dissolution phenomena and the observation of the super- saturation and the precipitation of pharmaceutical pro- ducts, which is useful information to predict in vivo dissolution of pharmaceutical products. This

关键词： GIS in vivo predictive dissolution ketocona-zole BCS subclassification supersaturation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

贮藏于4℃的家制酸奶中低浓度大肠杆菌O157:H7的存活研究

引用

食品科学 2010年第5期31卷 252-255页

作者：赖卫华冯贻泽 Beth ann Crozier-Dodson 南昌大学食品科学与技术国家重点实验室江西南昌330047 堪萨斯州立大学动物科学与工业系

牛奶预热后分装并分别接种体积分数3%的酸奶和2.15(lg(CFU/ml))的大肠杆菌O157:H7,在45℃下发酵5h后贮藏于4℃的冰箱中。利用Pathatrix大体积循环系统中偶联了抗体的免疫磁珠特异性地捕获这些酸奶中的大肠杆菌O157:H7。免疫磁珠重悬于1... 详细信息

牛奶预热后分装并分别接种体积分数3%的酸奶和2.15(lg(CFU/ml))的大肠杆菌O157:H7,在45℃下发酵5h后贮藏于4℃的冰箱中。利用Pathatrix大体积循环系统中偶联了抗体的免疫磁珠特异性地捕获这些酸奶中的大肠杆菌O157:H7。免疫磁珠重悬于1%的蛋白胨水后涂布于添加了新生霉素(5mg/l)的山梨醇麦康凯固体培养基中,培养基在37℃恒温培养箱中放置24h。实验结果表明,酸奶中大肠杆菌O157:H7的数量逐渐减少,12d后才检测不到。因此乳制品加工及保存过程中,需要加强对大肠杆菌O157:H7污染的监测,以保证乳制品的安全性。

关键词：大肠杆菌O157:H7 酸奶 Pathatrix系统

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Acute sensorineural hearing loss associated with peginterferon and ribavirin combination therapy during hepatitis C treatment: Outcome after resumption of therapy

引用

World Journal of Gastroenterology 2005年第34期11卷 5392-5393页

作者： Victor K Wong Cindy Cheong-lee Jo-ann E Ford Eric M Yoshida Department of Medicine Vancouver BC Canada University of British Columbia and the British Columbia Hepatitis Program Vancouver BC Canada Department of Medicine Vancouver BC Canada University of British Columbia and the British Columbia Hepatitis Program Vancouver BC Canada

Peginterferon and ribavirin combination therapy for the treatment of hepatitis C virus (HCV) is well known to be associated with significant adverse effects. Sensorineural hearing loss, that in most cases is unilateral, has been reported as a consequence of therapy with both non-pegylated and pegylated interferon (pegIFN) but is not a well-known adverse effect. We report a 45-year-old Caucasian woman who developed acute sensorineural hearing loss 2 mo after starting therapy with pegIFN-α 2b and ribavirin for the treatment of chronic HCV, genotype 1a. She did not report the hearing loss to the hepatitis clinic until l mo,later whereupon therapy was promptly *** her serum alanine aminotransferase (AlT)normalized and her HCV-RNA became undetectable after 12 wk of pegIFN and ribavirin therapy, after discontinuation,her HCV-RNA became detectable with significant elevations of serum AlT. Four months after initial discontinuation,the patient re-commenced pegIFN and ribavirin combination therapy. After 44 of 48 wk of therapy, the patient's liver biochemistry has normalized and the HCV-RNA is undetectable. She has not developed worsening of her hearing loss and hearing on the left-side is *** patients and physicians should be aware that sensorineural hearing loss may occur with pegIFN *** experience suggests that re-institution of therapy is not always associated with further hearing impairment.

关键词：急性感觉神经损伤听力损失病毒唑丙型肝炎病毒

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

一种联合肠道准备方案用于胶囊内镜检查:前瞻性随机对照研究

引用

Gastroenterology Report 2020年第1期8卷 31-35,I0001,I0002页

作者： Stephanie l.Hansel Joseph A.Murray Jeffrey A.Alexander David H.Bruining Mark V.larson Thomas F.Mangan Ross A.Dierkhising ann E.Almazar Elizabeth Rajan Division of Gastroenterology and Hepatology Mayo ClinicRochesterMNUSA Division of Biomedical Statistics and Informatics Mayo ClinicRochesterMNUSA

背景:胶囊内镜检查效果常受制于肠道内的食物残渣。本研究旨在评价一种联合肠道准备方安能否改善小肠视野,从而提高疾病诊断率和检查完成率。方法:本研究为前瞻性单盲随机对照研究,研究对象为在预约进行胶囊内镜检查的Mayo Clinic门诊... 详细信息

背景:胶囊内镜检查效果常受制于肠道内的食物残渣。本研究旨在评价一种联合肠道准备方安能否改善小肠视野,从而提高疾病诊断率和检查完成率。方法:本研究为前瞻性单盲随机对照研究,研究对象为在预约进行胶囊内镜检查的Mayo Clinic门诊患者。肠道准备组患者在胶囊内镜检查前夜饮用2 l聚乙二醇溶液,检查前20 min服用5ml西甲硅油和5mg胃复安,吞入胶囊内镜30 min后采用右侧卧位。对照组不做肠道准备,仅于检查前晚7点后禁食固体食物,检查前4 h内禁水。对所有研究对象进行满意度调查。胶囊内镜读片人员进行小肠视野评估。结果:前瞻性纳入50例患者,其中女性占56%,中位年龄54.4岁。44例完成研究,其中肠道准备组21例,对照组23例。两组患者在小肠四个分区中的视野清晰度的差异均无统计学意义(均P>0.05)。两组患者的疾病诊断率(P=0.69)、胃传输时间(P=0.10)和小肠传输时间(P=0.89)也均相当。肠道准备组的检查完成率显著高于对照组(100%vs 78%,P=0.02),但患者不适感也显著增多(62%vs 17%,P=0.01)。结论:联合肠道准备应用于胶囊内镜检查并没有改善小肠视野,却给患者带来了更多不适。尽管能提高检查完成率,但联合肠道准备并没有提高疾病检出率。因此,肠道准备似乎并不能改善胶囊内镜检查效果,反而会影响患者满意度。

关键词： bowel preparation capsule endoscopy small-bowel visualization patient satisfaction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bubbly shock propagation as a mechanism of shedding in separated cavitating flows

引用

Journal of Hydrodynamics 2017年第6期29卷 907-916页

作者： Harish Ganesh Simo A.Makiharju Steven l.Ceccio University of Michigan Ann ArborUSA University of California BerkeleyUSA

Stable attached partial cavitation in separated flows can transition to cloud shedding, and the mechanism of transition has been attributed to the presence of a re-entrant liquid jet. Our findings have revealed the presence of propagating bubbly shock waves as an alternative dominant mechanism of shedding when the compressibility of the bubbly mixture is appreciable. In the present paper, we discuss dynamics associated with these bubbly shock waves, interaction of shock waves with obstacles in their path, and means to manipulate their properties to control the shedding process by non-condensable gas injection.

关键词： Partial cavitation bubbly shock waves cloud cavitation X-ray densitometry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

THR EFFECT OF OXIDE lAYER FORMATION ON THE ADHESION OF CONDUCTIVE POlYMER COATING TO A MIlDSTEEl

引用

Acta Metallurgica Sinica(English letters) 1999年第4期12卷 421-426页

作者： Y. l. Zhang1) and G. Spinks2) 1) Departmentof Mechanical Engineering,Ngee ann Polytechnic,535 Clementi Road,Singapore5994892) Intelligent Polymer Research Institute, University of Wollongong, Northfields Ave, Wollongong, NSW, Australia,2522

Arolled mild steel wascoated byconductive polymers( polyaniline/ MMPand polyaniline/HCSA) andthenim mersedin hot water(50 ℃) fordifferenttimesforstudytheadhesion *** wasfound thatthe adhesion tendsto decrease over time. Thestudyshown that onebrittle and porous Fe3 O4 layer formed between conductive polymercoating and steelsub stratein a moistenvironmentand, asa result, decreased thecoating adhesions.

关键词： conductive polymercoating adhesion oxidelayer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Anthropogenic emission is the main contributor to the rise of atmospheric methane during 1993-2017

引用

National Science Review 2022年第5期9卷 154-166页

作者：张臻 Benjamin Poulter Sara Knox ann Stavert Gavin McNicol Etienne Fluet-Chouinard Aryeh Feinberg 赵园红 Philippe Bousquet Josep G.Canadell Anita Ganesan Gustaf Hugelius George Hurtt Robert B.Jackson Prabir K.Patra Marielle Saunois lena H?glund-Isaksson 黄春林 Abhishek Chatterjee 李新 Department of Geographical Sciences University of Maryland Biospheric Sciences laboratory NASA Goddard Space Flight Center Department of Geography University of British Columbia Global Carbon Project CSIRO Oceans and Atmosphere Department of Earth and Environmental Sciences University of Illinois Chicago Department of Earth System Science Stanford University Institute for Data Systems and Society Massachusetts Institute of Technology College of Oceanic and Atmospheric Sciences Ocean University of China laboratoire des Sciences du Climat et de l’Environment LSCE-IPSL (CEA-CNRS-UVSQ)Université Paris-Saclay School of Geographical Sciences University of Bristol Department of Physical Geography and Bolin Centre for Climate Research Stockholm University Woods Institute for the Environment and Precourt Institute for Energy Stanford University Research Institute for Global Change JAMSTEC International Institute for Applied Systems Analysis (IIASA) Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Global Modeling and Assimilation Office NASA Goddard Space Flight Center Universities Space Research Association Institute of Tibetan Plateau Research Chinese Academy of Sciences

Atmospheric methane(CH4) concentrations have shown a puzzling resumption in growth since 2007 following a period of stabilization from 2000 to *** hypotheses have been proposed to explain the temporal variations in CH4growth,and attribute the rise of atmospheric CH4either to increases in emissions from fossil fuel activities,agriculture and natural wetlands,or to a decrease in the atmospheric chemical ***,we use a comprehensive ensemble of CH4source estimates and isotopic δ13C-CH4source signature data to show that the resumption of CH4growth is most likely due to increased anthropogenic *** emission scenarios that have the fewest biases with respect to isotopic composition suggest that the agriculture,landfill and waste sectors were responsible for 53±13% of the renewed growth over the period 2007-2017 compared to 2000-2006;industrial fossil fuel sources explained an additional 34 ±24%,and wetland sources contributed the least at 13 ±9%.The hypothesis that a large increase in emissions from natural wetlands drove the decrease in atmospheric δ13C-CH4values cannot be reconciled with current process-based wetland *** finding suggests the need for increased wetland measurements to better understand the contemporary and future role of wetlands in the rise of atmospheric methane and climate *** findings highlight the predominant role of anthropogenic activities in driving the growth of atmospheric CH4concentrations.

关键词： greenhouse gas carbon cycle climate mitigation wetland methane isotope

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：