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Journal of Oceanology and Limnology 2019年第3期37卷 1071-1079页

作者： SHANGGUAN Jingbo xu anle HU Xiaowei LI Zhongbao Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment Xiamen 361021China Fisheries College Jimei UniversityXiamen 361021China

The marine bivalve Ruditapes philippinarum(Veneridae)has always been an economically important aquaculture *** this study,106 831 unigenes and 2 664 SSR loci(1 locus/40 sequences)were achieved from the de novo assembly *** all the SSRs,tri-nucleotides(46.40%)was the most,followed by di-nucleotides(32.43%).Meanwhile,AAC/GTT(19.82%)was the most common SSR loci *** polymorphism detection using 32 wild *** individuals,34 polymorphic and 3 monomorphic SSR loci were screened,and the genetic index of them was *** results show that PIC of 30 polymorphic SSR loci was at medium and high levels(PIC>0.25).However,there were five SSR polymorphic loci(***871423,MG871428,MG871429,MG871434,MG871435)deviating from the Hardy-Weinberg equilibrium after the Bonferroni correction(adjusted P=0.001 471).The Na value(number of alleles per locus)ranged from 2 to *** addition,the Ho(observed heterozygosities)and He(expected heterozygosities)were 0.100 0-1.000 0 and 0.191 3-0.723 6,***,RNA-Seq was shown as a fast and cost-effective method for genic SSR development in non-model ***,the 37 loci from *** will further enrich the genetic information and advance the population conservation and restoration.

关键词： Ruditapes philippinarum transcriptome microsatellite genetic diversity

DNA barcoding of fishes from Zhoushan coastal waters using mitochondrial COI and 12S rRNA genes

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Journal of Oceanology and Limnology 2023年第5期41卷 1997-2009页

作者： Yehui WANG Na SONG Shude LIU Zhi CHEN anle xu Tianxiang GAO Fisheries College Ocean University of ChinaQingdao 266003China Shandong Fisheries Development and Resources Conservation Center Yantai 264003China College of Fisheries and Life Science Hainan Tropical Ocean UniversitySanya 572022China School of Fishery Zhejiang Ocean UniversityZhoushan 316000China

Accurate species identification is a key component of biodiversity *** barcoding is an effective molecular method used for fish species *** aimed to study the DNA barcoding of fish in Zhoushan coastal waters,explore the differences and applicability of two gene fragments(12S rRNA and COI)of DNA barcoding in fish species identification,and established a comprehensive fish barcoding reference *** hundred and eighty-seven captured fish samples from Zhoushan coastal waters were identified using morphological characteristics and DNA barcoding.A total of 26412S rRNA sequences(belonging to eight orders,31 families,55 genera,and 66 species)and 188 COI sequences(belonging to seven orders,30 families,48 genera,and 58 species)were *** lengths of the 12S rRNA sequences ranged from 165 to 178 bp,and the guanine-cytosine(GC)content was 45.37%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.10%and 26.66%,*** length of the COI sequence ranged 574–655 bp,and the content of GC was 45.97%.The average 12S rRNA interspecific and intraspecific genetic distances(K2P)were 0.16%and 27.45%,*** minimum interspecific genetic distances of 12S rRNA and COI(1.23%and 1.86%)were both greater than their maximum intraspecific genetic distances(2.42%and 8.66%).Three molecular analyses(NJ tree,ABGD,and GMYC)were performed to accurately identify and delineate *** errors occurred when the 12S rRNA sequences were delimited using the NJ tree method,and the delimitation results of ABGD and GMYC are consistent with the final species identification *** results demonstrate that DNA barcoding based on 12S rRNA and COI can be used as an effective tool for fish species identification,and 12S rRNA has good application prospects in the environmental DNA(eDNA)metabarcoding of marine fish.

关键词： DNA barcoding cytochrome c oxidase subunit I(COI) 12S rRNA fish identification species delimitation Zhoushan coastal waters

Effects of Poria cocos polysaccharide on growth performance,physiological parameters,and lipid metabolism of spotted sea bass Lateolabrax maculatus

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Journal of Oceanology and Limnology 2024年第1期42卷 316-331页

作者： Jing LU Zhangfan HUANG Youling YE anle xu Zhongbao LI Fisheries College Jimei UniversityXiamen 361021China Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment Xiamen 361021China

The aquaculture industry has developed significantly over the past few decades and has had a substantial impact on the global food supply and marine fisheries ***,some problems arise behind the scenes due to excessive intensive farming,such as slow animal growth,frequent disease,and lipid metabolism *** problems have limited the sustainable development of the aquaculture industry,and a continuable solution is *** use of fungal polysaccharide appears to provide a solution to these ***,different supplemented levels of Poria cocos polysaccharide(PCP)(0,0.4,0.8,1.2,1.6,and 2.0 g/kg,respectively)were fed to spotted sea bass(Lateolabrax maculatus)in similar size(30.28±0.18 g)in current *** effects of PCP on growth,physiological parameters,and lipid metabolism of spotted sea bass were investigated after a 4-week rearing *** showed,fish with PCP intake presented a significantly higher weight gain,specific growth rate,and a significantly lower feed conversion *** higher trypsin activity in liver and intestine were observed in fish with PCP *** superoxide dismutase activity in serum and liver of fish with PCP intake were significantly improved,while significantly higher serum total antioxidant capacity and hepatic catalase activity were also ***,no significant differences in lysozyme and alkaline phosphatase activity were evident among *** with PCP intake showed a significantly lower total cholesterol,but no noteworthy change in triglyceride and lipid-metabolismrelated genes expression were observed among *** indicated that intake of PCP has a positive effect on growth and antioxidant capacity of spotted sea bass,but seems to have a limited effect on the non-specific immunity and lipid metabolism of spotted sea *** on the regression analysis results,1.4 g/kg of PCP is the optimal dose for spotted sea bass in size(30.28±0.18 g).

关键词： spotted sea bass Poria cocos polysaccharide growth lipid metabolism

High-Intensity Femtosecond Laser Interaction with Rare Gas Clusters

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Chinese Journal of Lasers 2001年第2期 38-43页

作者： Ni Guoquan Li Zhong Lei anle xu Zhizhan (Laboratory for High Intensity Optics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China)

With a 45 fs multiterawatt 790 nm laser system and jets of argon and krypton atomic clusters, a study of the interaction of fs intense laser pulses with large size rare gas clusters was conducted. The maximum laser intensity of about 7×10 16 W/cm 2 and clusters composed of thousands of atoms which were determined through Rayleigh scattering measurements were involved in the experiments. On the one hand, the results indicate that the interaction is strongly cluster size dependent. The stronger the interaction, the larger the clusters are. On the other hand, a saturation followed by a drop of the energy of ions ejected from the interaction will occur when the laser intensity exceeds a definite value for clusters of a certain size.

关键词： Ultrashort intense laser Clusters interaction gas jet

饲料中添加壳寡糖对花鲈(Lateolabrax japonicus)幼鱼的生长、消化和血清生化指标的影响

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海洋学报 2018年第2期40卷 69-76页

作者：胡晓伟上官静波黎中宝杨敏徐安乐集美大学水产学院福建厦门361021 福建省海洋渔业资源与生态环境重点实验室福建厦门361021

本试验旨在研究壳寡糖对花鲈(Lateolabrax japonicus)幼鱼生长,肠道消化,血清生化指标及肠道菌群的影响。本试验选用360尾规格一致的花鲈幼鱼(19.37g左右)为研究对象。壳寡糖饲料添加浓度设为6组:0%、0.2%、0.4%、0.6%、0.8%、1.0%。设... 详细信息

本试验旨在研究壳寡糖对花鲈(Lateolabrax japonicus)幼鱼生长,肠道消化,血清生化指标及肠道菌群的影响。本试验选用360尾规格一致的花鲈幼鱼(19.37g左右)为研究对象。壳寡糖饲料添加浓度设为6组:0%、0.2%、0.4%、0.6%、0.8%、1.0%。设3个平行组,养殖周期45d。结果显示:(1)生长性能:添加0.6%和0.8%两组的增重率、特定生长率与对照组相比有提高(P>0.05);(2)肠道消化酶:添加0.6%~1.0%的壳寡糖显著(P0.05),0.2%的壳寡糖增强超氧化物歧化酶、碱性磷酸酶和过氧化物酶活性,添加壳寡糖使溶菌酶的活性显著增强(P<0.05)。研究结果表明:饲料中添加0.6%~0.8%的壳寡糖对花鲈幼鱼生长性能、血脂及肠道健康影响效果最佳。

关键词：花鲈壳寡糖生长性能非特异性免疫肠道菌群

发酵浒苔对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响

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海洋学报 2018年第4期40卷 96-105页

作者：徐安乐黎中宝上官静波胡晓伟黄永春集美大学水产学院福建厦门361021 福建省海洋渔业资源与生态环境重点实验室福建厦门361021

浒苔经发酵后按添加比例(0%、1%、2%、3%、4%、5%)分别加入到珍珠龙胆石斑鱼(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀)基础饲料中配制成6种饲料,用以探讨发酵浒苔对珍珠龙胆石斑鱼生长、肠消化酶活性及血清非特异性免... 详细信息

浒苔经发酵后按添加比例(0%、1%、2%、3%、4%、5%)分别加入到珍珠龙胆石斑鱼(Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀)基础饲料中配制成6种饲料,用以探讨发酵浒苔对珍珠龙胆石斑鱼生长、肠消化酶活性及血清非特异性免疫力的影响。选用初始平均体质量为(19.16±0.25)g的珍珠龙胆石斑鱼为研究材料,试验周期28d。结果表明:发酵浒苔能显著影响石斑鱼的增重率(WGR)和特定生长率(SGR)(P0.05)。在添加组中,淀粉酶、脂肪酶以及胰蛋白酶活性均得到了显著提高(P<0.05),添加水平在2%~3%时效果最好。综合发酵浒苔对珍珠龙胆石斑鱼的作用效果可以看出,发酵浒苔可以明显促进其生长,提高非特异性免疫力及肠道中消化酶活力,建议发酵浒苔在饲料中添加量为2%左右。

关键词：发酵浒苔珍珠龙胆石斑鱼生长肠道消化酶非特异性免疫

低聚木糖对花鲈幼鱼生长性能、血清生化和免疫指标及肠道菌群组成的影响

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动物营养学报 2018年第2期30卷 734-742页

作者：胡晓伟上官静波黎中宝杨敏徐安乐集美大学水产学院厦门361021 福建省海洋渔业资源与生态环境重点实验室厦门361021

本试验旨在研究低聚木糖(XOS)对花鲈(Lateolabrax japonicus)幼鱼生长性能、血清生化和免疫指标及肠道菌群组成的影响。选用360尾花鲈幼鱼,随机分为6组,每组3个重复,每个重复20尾鱼。各组分别饲喂在基础饲料中添加0(对照组)、200(D200组... 详细信息

本试验旨在研究低聚木糖(XOS)对花鲈(Lateolabrax japonicus)幼鱼生长性能、血清生化和免疫指标及肠道菌群组成的影响。选用360尾花鲈幼鱼,随机分为6组,每组3个重复,每个重复20尾鱼。各组分别饲喂在基础饲料中添加0(对照组)、200(D200组)、400(D400组)、600(D600组)、800(D800组)和1 000 mg/kg低聚木糖(D1000组)的饲料。试验期45 d。结果表明:1)与对照组相比,D200、D400、D600组的增重率(WGR)和特定生长率(SGR)提高(P>0.05),D800(P>0.05)和D1000组(P0.05)。4)与对照组相比,D200、D400、D600、D800和D1000组的肠道中沙门氏菌数量显著降低(P<0.05),D200、D400和D1000组的肠道中大肠杆菌数量均显著降低(P<0.05),D200、D400和D600组的肠道中双歧杆菌数量显著增加(P<0.05)。由此可见,综合各试验指标,饲料中添加350~560 mg/kg的低聚木糖对花鲈幼鱼生长性能、免疫功能及肠道健康影响效果最佳。

关键词：花鲈生长性能低聚木糖非特异性免疫肠道菌群

基于线粒体控制区的中国东南沿海黄鳍棘鲷群体遗传多样性和遗传分化

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应用海洋学学报 2022年第4期41卷 583-590页

作者：叶幼玲黄张帆徐安乐汪睛黎中宝集美大学水产学院福建厦门361021 福建省海洋渔业资源与生态环境重点实验室福建厦门361021 福建省花鲈育种重点实验室福建福鼎355200

本研究通过对线粒体控制区序列进行分析的技术手段,对中国宁德(ND)、厦门(XM)、漳浦(ZP)、南澳岛(NAD)、湛江(ZJ)、海口(HK)与北海(BH)的7个野生黄鳍棘鲷(Acanthopagrus latus)群体的遗传多样性和遗传分化进行比较分析。结果表明:中国... 详细信息

本研究通过对线粒体控制区序列进行分析的技术手段,对中国宁德(ND)、厦门(XM)、漳浦(ZP)、南澳岛(NAD)、湛江(ZJ)、海口(HK)与北海(BH)的7个野生黄鳍棘鲷(Acanthopagrus latus)群体的遗传多样性和遗传分化进行比较分析。结果表明:中国东南沿海的野生黄鳍棘鲷群体呈现出中等以上的遗传多样性特征,48个黄鳍棘鲷样品的控制区序列长度为580 bp,单倍型数目为44个,总体的单倍型多样性指数(Hd)和核苷酸多样性指数(Pi)分别为0.996和0.01730。7个野生黄鳍棘鲷群体间的遗传分化程度较低,各群体间的遗传距离为0.01028~0.02987。群体间的遗传分化系数(F_(ST))为-0.06841~0.54586,北海群体和其他群体间存在一定程度的遗传分化。AMOVA分析显示群体内的遗传变异占比为83.34%,大部分遗传变异发生在组群内;但北海群体与其他6个群体分为两个组群后,组群间的遗传变异占比为42.66%,两个组群间存在较大程度的遗传变异。基于Kimrua距离法构建的系统进化树中显示北海群体聚为一个分支,另外6个群体聚为一支。基于TCS network构建的单倍型网络图中44个单倍型混杂在一起,未按照水系格局和地理距离进行分布,表明黄鳍棘鲷群体间未形成多个单系群。本研究可为中国东南沿海野生黄鳍棘鲷群体的种质资源利用和保护提供科学依据。

关键词：海洋生物学黄鳍棘鲷线粒体控制区遗传多样性遗传分化

复方中草药对珍珠龙胆石斑鱼生长、非特异性免疫及消化酶活性的影响

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海洋学报 2018年第12期40卷 49-57页

作者：徐安乐黎中宝上官静波胡晓伟黄永春林茂松林泽滨集美大学水产学院福建厦门361021 福建省海洋渔业资源与生态环境重点实验室福建厦门361021 福建省东山县祥源汇水产养殖有限公司福建东山363400

中草药含有多种活性物质,具有抗病助长无残留等特性,在国内外被广泛使用。本试验采用单因素试验设计方法,研究复方中草药(由黄芪、党参、大黄等12种中草药组合而成)不同剂量对珍珠龙胆石斑鱼(Epinephelus lanceolatus×Epinephelus fusc... 详细信息

中草药含有多种活性物质,具有抗病助长无残留等特性,在国内外被广泛使用。本试验采用单因素试验设计方法,研究复方中草药(由黄芪、党参、大黄等12种中草药组合而成)不同剂量对珍珠龙胆石斑鱼(Epinephelus lanceolatus×Epinephelus fuscoguttatus♀)生长性能、非特异性免疫及消化酶活性的影响。以初始体质量(19.16±0.25) g,体长为(10.63±0.56) cm的珍珠龙胆石斑鱼为试验对象,在基础饲料中分别添加0、0.4%、0.8%、1.2%、1.6%和2%复方中草药连续饲喂珍珠龙胆28 d。结果显示,该种复方中草药在添加剂量1.6%以内时,能有效降低石斑鱼的饵料系数(P0.05),但能显著提高石斑鱼血清中总蛋白含量(TP)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)以及总超氧化物歧化酶(T-SOD)活性(P0.05),中草药对珍珠龙胆石斑鱼肠道消化酶活性的影响在添加剂量为1.2%时尤为明显。综合石斑鱼的生长、非特异性免疫和消化性能结果,认为该种复方中草药的最适添加量为1.2%左右。

关键词：珍珠龙胆石斑鱼中草药生长性能消化酶非特异性免疫

Aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders:progress of experimental models based on disease pathogenesis

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Neural Regeneration Research 2025年第2期20卷 354-365页

作者： Li xu Huiming xu Changyong Tang Department of Neurology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong ProvinceChina

Neuromyelitis optica spectrum disorders are neuroinflammatory demyelinating disorders that lead to permanent visual loss and motor *** date,no effective treatment exists as the exact causative mechanism remains ***,experimental models of neuromyelitis optica spectrum disorders are essential for exploring its pathogenesis and in screening for therapeutic *** most patients with neuromyelitis optica spectrum disorders are seropositive for IgG autoantibodies against aquaporin-4,which is highly expressed on the membrane of astrocyte endfeet,most current experimental models are based on aquaporin-4-IgG that initially targets *** experimental models have successfully simulated many pathological features of neuromyelitis optica spectrum disorders,such as aquaporin-4 loss,astrocytopathy,granulocyte and macrophage infiltration,complement activation,demyelination,and neuronal loss;however,they do not fully capture the pathological process of human neuromyelitis optica spectrum *** this review,we summarize the currently known pathogenic mechanisms and the development of associated experimental models in vitro,ex vivo,and in vivo for neuromyelitis optica spectrum disorders,suggest potential pathogenic mechanisms for further investigation,and provide guidance on experimental model *** addition,this review summarizes the latest information on pathologies and therapies for neuromyelitis optica spectrum disorders based on experimental models of aquaporin-4-IgG-seropositive neuromyelitis optica spectrum disorders,offering further therapeutic targets and a theoretical basis for clinical trials.

关键词： aquaporin-4 experimental model neuromyelitis optica spectrum disorder pathogenesis