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High non-photochemical quenching of VPZ transgenic potato plants limits CO_(2) assimilation under high light conditions and reduces tuber yield under fluctuating light

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Journal of Integrative Plant Biology 2022年第9期64卷 1821-1832页

作者： Günter G.Lehretz anja schneider Dario Leister Uwe Sonnewald Department of Biology Division of BiochemistryFriedrich-Alexander-University Erlangen-NürnbergStaudtstrasse 5Erlangen91058 Germany Plant Molecular Biology Faculty of BiologyLudwig Maximilians University of MunichGroßhaderner Str.2-482152 Planegg-MartinsriedMunichGermany

Under natural conditions,photosynthesis has to be adjusted to fluctuating light *** exposed to high light dissipate excess light energy in form of heat at photosystem II(PSII)by a process called non-photochemical quenching(NPQ).Upon fast transition from light to shade,plants lose light energy by a relatively slow relaxation from *** overexpression of violaxanthin de-epoxidase(VDE),PSII subunit S(PsbS)and zeaxanthin epoxidase(ZEP)in tobacco accelerates relaxation from photoprotection,and increases photosynthetic *** Arabidopsis,expression of the same three genes(VPZ)resulted in a more rapid photoprotection but growth of the transgenic plants was *** we report on VPZ expressing potato plants grown under various light *** to tobacco and Arabidopsis,induction and relaxation of NPQ was accelerated under all growth conditions tested,but did not cause an overall increased photosynthetic rate or growth of transgenic *** yield of VPZ expressing plants was unaltered as compared to control plants under constant light conditions and even decreased under fluctuating light *** control conditions,levels of the phytohormone abscisic acid(ABA)were found to be elevated,indicating an increased violaxanthin availability in VPZ ***,the increased basal ABA levels did not improve drought tolerance of VPZ transgenic potato plants under greenhouse *** failure to benefit from improved photoprotection is most likely caused by a reduced radiation use efficiency under high light conditions resulting from a too strong NPQ *** this negative effect in the future might help to improve photosynthetic performance in VPZ expressing potato plants.

关键词： drought photosynthesis potato non-photochemical quenching xanthophyll cycle

The Plastid Envelope CHLOROPLAST MANGANESE TRANSPORTER1 Is Essential for Manganese Homeostasis in Arabidopsis

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Molecular Plant 2018年第7期11卷 955-969页

作者： Marion Eisenhut Natalie Hoecker Sidsel Birkelund Schmidt Rubek Merina Basgaran Samantha Flachbart Peter Jahns Tabea Eser Stefan Geimer Seren Husted Andreas P.M. Weber Dario Leister anja schneider Biochemie der Pflanzen Cluster of Excellence on Plant Science (CEPLAS) Heinrich-Heine-University Di3sseldorf 40225 Desseldorf Germany Molekularbiologie der Pflanzen (Botanik) Department Biologie I Ludwig-Maximilians-University Mernchen 82152 Martinsried Germany Department of Plant and Environmental Sciences and Copenhagen Plant Science Centre (CPSC) Faculty of Science University of Copenhagen 1871 Frederiksberg Denmark Zellbiologie/Elektronenmikroskopie NW I/B1 Universit&t Bayreuth 95447 Bayreuth Germany These authors contributed equally to this article.

The transition metal manganese （Mn） is indispensable for photoautotrophic growth since photosystem II （PSII） employs an inorganic Mn4CaOs cluster for water splitting. Here, we show that the Arabidopsis membrane protein CHLOROPLAST MANGANESE TRANSPORTER1 （CMT1） is involved in chloroplast Mn homeostasis. CMT1 is the closest homolog of the previously characterized thylakoid Mn transporter PHOTOSYNTHESIS-AFFECTED MUTANT71 （PAM71）. In contrast to PAM71, CMT1 resides at the chloro- plast envelope and is ubiquitously expressed. Nonetheless, like PAM71, the expression of CMT1 can also alleviate the Mn-sensitive phenotype of yeast mutant qomrl. The cmtl mutant is severely suppressed in growth, chloroplast ultrastructure, and PSII activity owing to a decrease in the amounts of pigments and thylakoid membrane proteins. The importance of CMT1 for chloroplast Mn homeostasis is demonstratedby the significant reduction in chloroplast Mn concentrations in cmtl-1, which exhibited reduced Mn binding in PSII complexes. Moreover, CMT1 expression is downregulated in Mn-surplus conditions. The pam71 cmtl-ldouble mutant resembles the cmtl-f single mutant rather than pare71 in most respects. Taken together, our results suggest that CMT1 mediates Mn2 uptake into the chloroplast stroma, and that CMT1 and PAM71 function sequentially in Mn delivery to PSII across the chloroplast envelope and the thylakoid membrane.

关键词： manganese transporter envelope chloroplast photosynthesis Arabidopsis

APPROXIMATING THE STATIONARY BELLMAN EQUATION BY HIERARCHICAL TENSOR PRODUCTS

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Journal of Computational Mathematics 2024年第3期42卷 638-661页

作者： Mathias Oster Leon Sallandt Reinhold schneider Technische Universität Berlin Strasse des 17.Juni 13510623 BerlinGermany

We treat infinite horizon optimal control problems by solving the associated stationary Bellman equation numerically to compute the value function and an optimal feedback *** dynamical systems under consideration are spatial discretizations of non linear parabolic partial differential equations(PDE),which means that the Bellman equation suffers from the curse of *** non linearity is handled by the Policy Iteration algorithm,where the problem is reduced to a sequence of linear equations,which remain the computational bottleneck due to their high *** reformulate the linearized Bellman equations via the Koopman operator into an operator equation,that is solved using a minimal residual *** the Koopman operator we identify a preconditioner for operator equation,which deems essential in our numerical *** overcome computational infeasability we use low rank hierarchical tensor product approximation/tree-based tensor formats,in particular tensor trains(TT tensors)and multi-polynomials,together with high-dimensional quadrature,*** controlling a destabilized version of viscous Burgers and a diffusion equation with unstable reaction term numerical evidence is given.

关键词： Feedback control Dynamic programming Hamilton-Jacobi-Bellman Tensor product approximation Variational Monte-Carlo

Is the speed of adjusting to environmental change condition dependent?An experiment with house mice(Mus musculus)

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Current Zoology 2024年第3期70卷 350-360页

作者： Karem Lopez-Hervas Neelam Porwal Mathilde Delacoux Alexandros Vezyrakis anja Guenther RG Behavioural Ecology of Individual Differences Max Planck Institute for Evolutionary Biology24306 PlönGermany Department of Evolutionary Biology Faculty of BiologyAdam Mickiewicz UniversityWieniawskiego 161-712 PoznańPoland Department for Collective Behaviour Max Planck Institute of Animal Behaviour78464 ConstanceGermany Centre for the Advanced Study of Collective Behaviour University of Konstanz78464 ConstanceGermany Animal Ecology Institute for Biochemistry and BiologyUniversity of Potsdam14469 PotsdamGermany

Environmental conditions change constantly either by anthropogenic perturbation or naturally across space and ***,a change in behavior is the frst response to changing *** fexibility can potentially improve an organism’s chances to survive and ***,we lack an understanding on the time-scale such behavioral adjustments need,how they actually affect reproduction and survival and whether behavioral adjustments are suffcient in keeping up with changing *** used house mice(Mus musculus)to test whether personality and life-history traits can adjust to an experimentally induced food-switch fexibly in adulthood or by intergenerational plasticity,that is,adjustments only becoming visible in the offspring *** lived in 6 experimental populations of semi-natural environments either on high or standard quality food for 4 *** showed previously that high-quality food induced better conditions and a less risk-prone ***,we tested whether the speed and/or magnitude of adjustment shows condition-dependency and whether adjustments incur ftness ***-history but not personality traits reacted fexibly to a food-switch,primarily by a direct reduction of reproduction and sloweddown *** whose parents received a food-switch developed a more active stress-coping personality and gained weight at a slower rate compared with their respective ***,the modulation of most traits was condition-dependent,with animals previously fed with high-quality food showing stronger *** study highlights that life-history and personality traits adjust at different speed toward environmental change,thus,highlighting the importance of the environment and the mode of response for evolutionary models.

关键词： animal personality context dependence environmental change Match-mismatch non-genetic inheritance phenotypic flexibility phenotypic plasticity

Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α

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Synthetic and Systems Biotechnology 2024年第3期9卷 416-424页

作者： Jeffrey L.Schloßhauer Lena Tholen Alexander Korner Stefan Kubick Sofia Chatzopoulou anja Honow Anne Zemella Fraunhofer Project Group PZ-Syn of the Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Faculty of Health Sciences Joint Faculty of the Brandenburg University of Technology Cottbus-SenftenbergThe Brandenburg Medical School Theodor FontaneUniversity of PotsdamPotsdamGermany Institute for Chemistry and Biochemistry Laboratory of Protein BiochemistryFreie Universitat BerlinThielallee 6314195BerlinGermany Institute of Biotechnology Technische Universit¨at BerlinStraße des 17.Juni 13510623BerlinGermany New/era/mabs GmbH August-Bebel-Str.8914482PotsdamGermany

Chinese hamster ovary(CHO)cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational ***,toxic proteins and membrane proteins are often difficult-to-express in living ***,cell-free protein synthesis can be *** study explores innovative strategies for enhancing the production of challenging proteins through the modification of CHO cells by investigating both,cell-based and cell-free approaches.A major result in our study involves the integration of a mutant eIF2 translation initiation factor and T7 RNA polymerase into CHO cell lysates for cell-free protein *** resulted in elevated yields,while eliminating the necessity for exogenous additions during cell-free production,thereby substantially enhancing ***,we explore the potential of the Rosa26 genomic site for the integration of T7 RNA polymerase and cell-based tetracycline-controlled protein *** findings provide promising advancements in bioproduction technologies,offering flexibility to switch between cell-free and cell-based protein production as needed.

关键词： Inducible expression CHO cells Cell-free protein synthesis CRISPR T7 RNA polymerase eIF2 Rosa26

Modeling displacement flow inside a full-length casing string for well cementing

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Petroleum Research 2024年第1期9卷 1-16页

作者： Hu Dai Ali Eslami Jason schneider Gefei Liu Fred Schwering Pegasus Vertex Inc.USA Laval University Canada Sanjel Energy Services Canada Athabasca Oil Corporation Canada

While computer modeling of annular displacement efficiency is widely applied in cementing engineering,modeling the displacement flow inside a casing or drill string for cementing operations has received less *** predicting displacement efficiency inside a full-length pipe is desired by cementing engineers,the attempt of developing a model with both efficiency and accuracy faces *** to computer simulators for this purpose is *** with annular flow,the displacement flow inside pipe,although within a simpler geometry and without eccentricity effect,is not simpler in physics,modelling strategy and predictability,because a variety of flow patterns and flow instabilities can develop to create complicated fluid *** this paper,we present an integrated numerical model developed to simulate displacement flows inside a full-length pipe,which connects an existing annulus model to enable complete displacement simulations of cementing *** model uses three-dimensional grid to solve fluid concentrations with degrees of mixing,and incorporates flow instability detection and flow regime *** in cementing,the model accounts for effects of pumping rate,well inclination,pipe rotation,fluid densities,rheological parameters and *** computationally efficient model does not rely on high-resolution mesh as often required by conventional Computational Fluid Dynamics models,thus it is suitable to be implemented in a cementing software for daily use by well cementing *** methodology of the model is discussed in detail in this *** validate the model,we examine simulation results against experimental results obtained in our laboratory tests and CFD simulations;acceptable agreement is found under different testing *** also presented two case studies of real cementing jobs with cement evaluation logs compared to simulation results,showing that the model can predict consistent displacement efficie

关键词： Inside pipe displacement Displacement efficiency Numerical model Primary cementing Displacement flow

Two-photon emission from a superlattice-based superconducting light-emitting structure

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Light(Science & Applications) 2024年第7期13卷 1331-1340页

作者： Shlomi Bouscher Dmitry Panna Ronen Jacovi Fauzia Jabeen Christian schneider Sven Höfling Alex Hayat Department of Electrical Engineering TechnionHaifa 32000Israel. Technische Physik Universität WürzburgAm HublandD-97074 WürzburgGermany. Institute of Physics Carl von Ossietzky Universität OldenburgD-26111 OldenburgGermany

Superconductor-semiconductor hybrid devices can bridge the gap between solid-state-based and photonics-based quantum systems,enabling new hybrid computing schemes,offering increased scalability and *** example for a hybrid device is the superconducting light-emitting diode(SLED).SLEDs have been theoretically shown to emit polarization-entangled photon pairs by utilizing radiative recombination of Cooper ***,the twophoton nature of the emission has not been shown experimentally *** demonstrate two-photon emission in a GaAs/AlGaAs *** electroluminescence spectra reveal unique two-photon superconducting features below the critical temperature(Tc),while temperature-dependent photon-pair correlation experiments(g(2)(τ,T))demonstrate temperature-dependent time coincidences below Tc between photons emitted from the *** results pave the way for compact and efficient superconducting quantum light sources and open new directions in light-matter interaction studies.

关键词： photon quantum utilizing

Efficient scar-free knock-ins of several kilobases in plants by engineered CRISPR-Cas endonucleases

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Molecular Plant 2024年第5期17卷 824-837页

作者： Tom Schreiber anja Prange Petra Schafer Thomas Iwen Ramona Grutzner Sylvestre Marillonnet Aurelie Lepage Marie Javelle Wyatt Paul Alain Tissier Department of Cell and Metabolic Biology Leibniz Institute of Plant BiochemistryWeinberg 306120 Halle(Saale)Germany Limagrain Centre de RechercheRoute d’EnnezatCS 9012663720 ChappesFrance

In plants and mammals,non-homologous end-joining is the dominant pathway to repair DNA doublestrand breaks,making it challenging to generate knock-in *** this study,we identified two groups of exonucleases from the herpes virus and the bacteriophage T7 families that conferred an up to 38-fold increase in homology-directed repair frequencies when fused to Cas9/Cas12a in a tobacco mosaic virus-based transient assay in Nicotiana *** achieved precise and scar-free insertion of several kilobases of DNA both in transient and stable transformation *** Arabidopsis thaliana,fusion of Cas9 to a herpes virus family exonuclease led to 10-fold higher frequencies of knock-ins in the first generation of *** addition,we demonstrated stable and heritable knock-ins in wheat in 1%of the primary *** together,our results open perspectives for the routine production of heritable knock-in and gene replacement events in plants.

关键词： homology-directed repair knockin gene replacement CRISPR-Cas 50 exonuclease plants

Role of homologous recombination/recombineering on human adenovirus genome engineering:Not the only but the most competent solution

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Engineering Microbiology 2024年第1期4卷 90-107页

作者： Lisa-Marie Dawson Montaha Alshawabkeh Katrin Schröer Fatima Arakrak anja Ehrhardt Wenli Zhang Virology and Microbiology Center for Biomedical Education and Research(ZBAF)School of MedicineFaculty of HealthWitten/Herdecke UniversityStockumer Str.1058453 WittenGermany

Adenoviruses typically cause mild illnesses,but severe diseases may occur primarily in immunodeficient individuals,particularly ***,adenoviruses have garnered significant interest as a versatile tool in gene therapy,tumor treatment,and vaccine vector *** the past two decades,the advent of recombineering,a method based on homologous recombination,has notably enhanced the utility of adenoviral vectors in therapeutic *** review summarizes recent advancements in the use of human adenoviral vectors in medicine and discusses the pivotal role of recombineering in the development of these ***,it highlights the current achievements and potential future impact of therapeutic adenoviral vectors.

关键词： Adenoviral vector Gene therapy Homologous recombination Oncolytic virus Vaccine vector