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International Journal of Geosciences 2013年第1期4卷 129-145页

作者： Luisa Galgani anja Engel Alfred Wegener Institute for Polar and Marine Research Bremerhaven Germany Helmholtz Centre for Ocean Research Kiel (GEOMAR) Kiel Germany

Since the early 80’s, the sea-surface microlayer (SML) has been hypothesized as being a gelatinous film. Recent studies have confirmed this characteristic, which confers properties that mediate mass and energy fluxes between ocean and atmosphere, including the emission of primary organic aerosols from marine systems. We investigated SML thickness and composition in five replicate indoor experiments between September and December 2010. During each experiment, the SML and underlying seawater were sampled from four seawater tanks: one served as control, and three were inoculated with Thalassiosira weissflogii grown in chemostats at 180, 380 and 780 ppm pCO2. We examined organic material enrichment factors in each tank, paying particular attention to gel particles accumulation such as polysaccharidic Transparent Exopolymer Particles (TEP) and the proteinaceous Coomassie Stainable Particles (CSP). While previous studies have observed carbohydrates and TEP enrichment in the microlayer, little is yet known about proteinaceous gel particles in the SML. Our experiments show that CSP dominate the gelatinous composition of the SML. We believe that the enrichment in CSP points to the importance of bacterial activity in the microlayer. Bacteria may play a pivotal role in mediating processes at the air-sea interface thanks to their exudates and protein content that can be released through cell disruption.

关键词： Sea Surface Microlayer Extracellular Polymeric Substances Organic Matter Bacterial Abundance Polysaccharides TEP CSP

MicroRNA-regulated viral vectors for gene therapy

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World Journal of Experimental Medicine 2016年第2期6卷 37-54页

作者： anja Geisler Henry Fechner Department of Applied Bioche-mistry Institute of Biotechnology Technische Universitat Berlin

Safe and effective gene therapy approaches require targeted tissue-specific transfer of a therapeutic *** traditional approaches, such as transcriptional and transductional targeting, micro RNA-dependent posttranscriptional suppression of transgene expression has been emerging as powerful new technology to increase the specificity of vector-mediated transgene expression. Micro RNAs are small non-coding RNAs and often expressed in a tissue-, lineage-, activation- or differentiation-specific pattern. They typically regulate gene expression by binding to imperfectly complementary sequences in the 3' untranslated region(UTR) of the m RNA. To control exogenous transgene expression, tandem repeats of artificial micro RNA target sites are usually incorporated into the 3' UTR of the transgene expression cassette, leading to subsequent degradation of transgene m RNA in cel s expressing the corresponding micro RNA. This targeting strategy, first shown for lentiviral vectors in antigen presenting cells, has now been used for tissue-specific expression of vector-encoded therapeutic transgenes, to reduce immune response against the transgene, to control virus tropism for oncolytic virotherapy, to increase safety of live attenuated virus vaccines and to identify and select cell subsets for pluripotent stem cell therapies, respectively. This review provides an introduction into the technical mechanism underlying micro RNA-regulation, highlights new developments in this field and gives an overview of applications of micro RNA-regulated viral vectors for cardiac, suicide gene cancer and hematopoietic stem cell therapy, as well as for treatment of neurological and eye diseases.

关键词： Micro RNA Micro RNA regulation Micro RNA target sites Viral vectors Adeno-associated virus RNA interference Gene therapy Vector targeting

Supramolecular Single-Chain Polymeric Nanoparticles

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CCS Chemistry 2019年第1期1卷 64-82页

作者： Gijs M.ter Huurne anja R.A.Palmans E.W.Meijer Institute for Complex Molecular Systems Laboratory of Macromolecular and Organic ChemistryEindhoven University of TechnologyEindhoven 5600 MBThe Netherlands

Nature has unparalleled control over the conforma-tion and dynamics of its folded macromolecular ***’s ability to arrange amino acids into a precise spatial organization by way of folding allows proteins to fulfill s... 详细信息

关键词： folding polymers enzyme mimics supra-molecular polymer chemistry nanoparticle

Microplastic fiber and drought effects on plants and soil are only slightly modified by arbuscular mycorrhizal fungi

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Soil Ecology Letters 2022年第1期4卷 32-44页

作者： Anika Lehmann Eva FLeifheit Linshan Feng Joana Bergmann anja Wulf Matthias C.Rillig Freie Universität Berlin Institut für Biologie14195 BerlinGermany Berlin-Brandenburg Institute of Advanced Biodiversity Research 14195 BerlinGermany Leibniz Centre for Agricultural Landscape Research(ZALF) 15374 MünchebergGermany

Microplastics are increasingly recognized as a factor of global change. By altering soil inherentproperties and processes, ripple-on effects on plants and their symbionts can be ***, interactions with other factors of global change, such as drought, can influence theeffect of microplastics. We designed a greenhouse study to examine effects of polyester microfibers,arbuscular mycorrhizal (AM) fungi and drought on plant, microbial and soil responses. We found thatpolyester microfibers increased the aboveground biomass of Allium cepa under well-watered anddrought conditions, but under drought conditions the AM fungal-only treatment reached the highestbiomass. Colonization with AM fungi increased under microfiber contamination, however, plantbiomass did not increase when both AM fungi and fibers were present. The mean weight diameter ofsoil aggregates increased with AM fungal inoculation overall but decreased when the system wascontaminated with microfibers or drought stressed. Our study adds additional support to themounting evidence that microplastic fibers in soil can affect the plant–soil system by promoting plantgrowth, and favoring key root symbionts, AM fungi. Although soil aggregation is usually positivelyinfluenced by plant roots and AM fungi, and microplastic promotes both, our results show that plasticstill had a negative effect on soil aggregates. Even though there are concerns that microplastic mightinteract with other factors of global change, our study revealed no such effect for drought.

关键词： Arbuscular mycorrhizal fungi Microplastic Soil aggregation Organic matter decomposition Drought Root traits

The Adaptive Solar Facade： From concept to prototypes

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Frontiers of Architectural Research 2016年第2期5卷 143-156页

作者： Zoltan Nasy Bratislav Svetozarevic Praseeth Jayathissa Moritz Begle Johannes Hofer Gearoid Lydon anja Willmann Arno Schlueter Architecture and Building Systems ETH ZOrich John-von-Neumann-Weg 9 8093 ZOrich Switzerland

The Adaptive Solar Facade （ASF） is a modular, highly integrated dynamic building *** energetic behavior as well as the architectural expression of the facade can be controlled with high spatio-temporal resolution through individually addressable modules. We present the general design process, the current mechanical design, and simulation results on photovoltaic power production and building energy consumption. We introduce the controller concept and show results on solar tracking as well as user interaction. Lastly, we present our current and planned prototypes.

关键词： Dynamic facade BIPV Facade engineering Photovoltaics Responsivearchitecture

Influence of bladder neck suspension stitches on early continence after radical prostatectomy： a prospective randomized study of 180 patients

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Asian Journal of Andrology 2011年第6期13卷 806-811页

作者： Jens-Uwe Stolzenburg Martin Nicolaus Panagiotis Kallidonis Minh Do anja Dietel Tim Haifner George Sakellaropoulos James Hicks David Nikoleishvili Evangelos Liatsikos Department of Urology University of Leipzig Leipzig 04103 Germany Department of Urology University of Patras Medical School Patras 26500 Greece Department of Medical Physics University of Patras Patras 26500 Greece

Several techniques have been introduced to improve early postoperative continence. In this study, we evaluated the impact of bladder neck （vesicourethral anastomosis） suspension on the outcome of extraperitoneal endoscopic radical prostatectomy （EERPE）. In this research, a total of 180 patients underwent EERPE. Group 1 included patients who underwent nerve-sparing EERPE （nsEERPE）（n=45）, and Group 2 included patients who underwent nsEERPE with bladder neck suspension （BNS, n=45）. Groups 3 （n=45） and 4 （n=45） included patients who received EERPE and EERPE with BNS, respectively. Patients were randomly assigned to receive BNS with their nsEERPE or EERPE procedure. Perioperative parameters were recorded, and continence was evaluated by determining the number and weight of absorbent pads （pad weighing test） on the second day after catheter removal and by a questionnaire 3 months postoperatively. Two days after catheter removal, 11.1% of Group 1, 11.1% of Group 2, 4.4% of Group 3 and 8.9% of Group 4 were conti nent. The average urine loss was 80.4, 70.1, 325.0 and 291.3 g for the each of these groups, respectively. At 3 months, 76.5% of Group I and 81.3% of Group 2 were continent. The continence figures for Group 3 and 4 were 48.5% and 43.8%, respectively. Similar overall rates were observed in all groups. In conclusion, although there are controversial reports in the literature, early continence was never observed to be significantly higher in the BNS groups when compared with the non-BNS groups, regardless of the EERPE technique performed.

关键词： bladder suspension extraperitoneal endoscopic radical prostatectomy nerve-sparing positive surgical margins prostatectomy vesicourethral anastomosis

Treatment of critical bone defects using calcium phosphate cement and mesoporous bioactive glass providing spatiotemporal drug delivery

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Bioactive Materials 2023年第10期28卷 402-419页

作者： Richard Frank Richter Corina Vater Margarete Korn Tilman Ahlfeld Martina Rauner Winnie Pradel Bernd Stadlinger Michael Gelinsky anja Lode Paula Korn Centre for Translational Bone Joint and Soft Tissue ResearchUniversity Hospital Carl Gustav Carus and Faculty of MedicineTechnische Universitat DresdenDresdenGermany Department of Oral and Maxillofacial Surgery University Hospital Carl Gustav CarusTechnische Universitat DresdenGermany Department of Medicine III and Center for Healthy Aging University Hospital Carl Gustav CarusTechnische Universitat DresdenGermany Clinic of Cranio-Maxillofacial and Oral Surgery Center of Dental MedicineUniversity of ZurichSwitzerland

Calcium phosphate cements (CPC) are currently widely used bone replacement materials with excellent bioactivity, but have considerable disadvantages like slow degradation. For critical-sized defects, however, an improved degradation is essential to match the tissue regeneration, especially in younger patients who are still growing. We demonstrate that a combination of CPC with mesoporous bioactive glass (MBG) particles led to an enhanced degradation in vitro and in a critical alveolar cleft defect in rats. Additionally, to support new bone formation the MBG was functionalized with hypoxia conditioned medium (HCM) derived from rat bone marrow stromal cells. HCM-functionalized scaffolds showed an improved cell proliferation and the highest formation of new bone volume. This highly flexible material system together with the drug delivery capacity is adaptable to patient specific needs and has great potential for clinical translation.

关键词： Drug delivery 3D plotting Alveolar cleft defect Calcium phosphate cement Mesoporous bioactive glass

The chemokine receptor CCR7 is a promising target for rheumatoid arthritis therapy

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Cellular & Molecular Immunology 2019年第10期16卷 791-799页

作者： Georgios L.Moschovakis anja Bubke Michaela Friedrichsen Jasmin Ristenpart Jaap Willem Back Christine S.Falk Elisabeth Kremmer Reinhold Forster Institute of Immunology Hannover Medical SchoolCarl-Neuberg Str.130625 HannoverGermany Pepscan Therapeutics BV LelystadThe Netherlands Institute of Transplant Immunology Integrated Research and Treatment Center TransplantationIFB.TxHannover Medical SchoolCarl-Neuberg Str.130625 HannoverGermany Helmholtz-Zentrum München Institute of Molecular ImmunologyD-81377 MunichGermany Present address:Biozentrum Martinsried Dept.Bio II.LMU MünchenGrosshaderner Str.2D-82152 MartinsriedGermany

The chemokine receptor CCR7 and its ligands CCL19 and CCL21 guide the homing and positioning of dendritic and T cells in lymphoid organs,thereby contributing to several aspects of adaptive immunity and immune *** the present study,we investigated the role of CCR7 in the pathogenesis of collagen-induced arthritis(CIA).By using a novel anti-human CCR7 antibody and humanized CCR7 mice,we evaluated CCR7 as a target in this autoimmune model of rheumatoid arthritis(RA).Ccr7-deficient mice were completely resistant to CIA and presented severely impaired antibody responses to collagen II(CII).Selective CCR7 expression on dendritic cells restored arthritis severity and anti-CII antibody *** and therapeutic treatment of humanized CCR7 mice with anti-human CCR7 mAb 8H3-16A12 led to complete resistance to CIA and halted CIA progression,*** data demonstrate that CCR7 signaling is essential for the induction of CIA and identify CCR7 as a potential therapeutic target in RA.

关键词： CCR7 impaired immunity

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Florence

Florence

Histologically proven hepatic steatosis associates with lower testosterone levels in men with obesity

作者： Introductions and Essays by Ross King Painting Descriptions by anja Grebe.

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Asian Journal of Andrology 2020年第3期22卷 252-257页

作者： Frederique Van de Velde Marlies Bekaert Anne Hoorens anja Geerts Guy T'Sjoen Tom Fiers Jean-Marc Kaufman Yves Van Nieuwenhove Bruno Lapauw department of Endocrinology Ghent University Hospital9000 GhentBelgium department of Pathology Ghent University Hospital9000 GhentBelgium Department of Hepathology Ghent University Hospital9000 GhentBelgium Department of Clinical Biology Ghent University Hospital9000 GhentBelgium Department of Gastro-Intestinal Surgery Ghent University Hospital9000 GhentBelgium.

Men with obesity often present with low testosterone(T)and sex hormone-binding globulin(SHBG)*** mechanisms for this have been proposed,but as SHBG is secreted by hepatocytes and sex steroids undergo hepatic metabolization,this study investigates whether severity and histological components of nonalcoholic fatty liver disease(NAFLD)are associated with sex steroid levels in obese *** cross-sectional study included 80 obese men(age:46±11 years;body mass index:42.2±5.5 kg m^-2).Serum levels of total T and estradiol(E.)were measured using liquid chromatography coupled with tandem mass spectroscopy(LC/MS-MS)and SHBG and gonadotropins by *** biopsies were evaluated using ***,and Fibrosis *** with steatohepatitis had similar median(1st quartile-3rd quartile)total T levels(7.6[5.0-11.0]nmol l^-1 vs 8.2[7.2-10.9]nmol l^-1;P=0.147),lower calculated free T(cFT)levels(148.9[122.9-188.8]pmol 1-1 s 199.5[157.3-237.6] pmol l^-1;P=0.006),and higher free E.T ratios(10.0[6.4-13.9]×10^-3 vs 7.1[5.7-10.7]×10^-3;P=0.026)compared to men with only nonalcoholic fatty *** the histological components of *** steatosis was independently associated with total T(r=-0.331.P=0.003)and cFT levels(r=-0.255.P=0.025).Obese men *** have even lower cFT levels compared to those without,an association mainly driven by grade of *** this reflects a subgroup of men with a more severe obesity-related phenotype or results from direct relations between hepatic steatosis and sex steroid metabolism needs further investigation.

关键词： male hypog on adism non alcoholic fatty liver disease obesity sex steroids steatosis testoster one