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Valproic acid and nonalcoholic fatty liver disease: A possible association?

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World Journal of Hepatology 2015年第9期7卷 1251-1257页

作者： Edoardo Farinelli David Giampaoli anja Cenciarini Ephraim Cercado Alberto Verrotti Department of Pediatrics University of Perugia General Surgery Department Lung Center of the Philippines Quezon Avenue Diliman Quezon City 1101Metro Manila Philippines

Valproic acid(VPA) is one of the most prescribed drugs in children with newly diagnosed epilepsy. Weight gain and obesity have been observed as side effects of VPA. These are often linked with other metabolic disturbances such as development of insulin resistance, dyslipidemia, metabolic syndrome(Met S) and nonalcoholic fatty liver disease or nonalcoholic fatty liver disease(NAFLD). NAFLD refers to a group of liver disorders with marked hepatic steatosis. It is associated with an increased incidence of cardiovascular diseases and overall reduced life expectancy. NAFLD occurs in 20%-25% of the general population and it is known to be the most common cause of chronic liver disease. NAFLD therefore represents a major public health issue worldwide. This study reviews and summarizes relevant literature that supports the existence of an association between VPA therapy and the development of NAFLD in children. Long-term VPA-therapy appears to be associated with an increased risk of developing NAFLD. Further studies are needed to clarify the pathogenic mechanisms that lie behind this association and to standardize the options for the use of this drug in overweight patients and in those with risks for developing Met S and NAFLD.

关键词： Nonalcoholic fatty liver disease Valproicacid Obesity Insulin resistance

Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α

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Synthetic and Systems Biotechnology 2024年第3期9卷 416-424页

作者： Jeffrey L.Schloßhauer Lena Tholen Alexander Korner Stefan Kubick Sofia Chatzopoulou anja Honow Anne Zemella Fraunhofer Project Group PZ-Syn of the Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Fraunhofer Institute for Cell Therapy and Immunology-IZI Branch Bioanalytics and Bioprocesses-IZI-BBAm MühlenbergPotsdamGermany Faculty of Health Sciences Joint Faculty of the Brandenburg University of Technology Cottbus-SenftenbergThe Brandenburg Medical School Theodor FontaneUniversity of PotsdamPotsdamGermany Institute for Chemistry and Biochemistry Laboratory of Protein BiochemistryFreie Universitat BerlinThielallee 6314195BerlinGermany Institute of Biotechnology Technische Universit¨at BerlinStraße des 17.Juni 13510623BerlinGermany New/era/mabs GmbH August-Bebel-Str.8914482PotsdamGermany

Chinese hamster ovary(CHO)cells are crucial in biopharmaceutical production due to their scalability and capacity for human-like post-translational ***,toxic proteins and membrane proteins are often difficult-to-express in living ***,cell-free protein synthesis can be *** study explores innovative strategies for enhancing the production of challenging proteins through the modification of CHO cells by investigating both,cell-based and cell-free approaches.A major result in our study involves the integration of a mutant eIF2 translation initiation factor and T7 RNA polymerase into CHO cell lysates for cell-free protein *** resulted in elevated yields,while eliminating the necessity for exogenous additions during cell-free production,thereby substantially enhancing ***,we explore the potential of the Rosa26 genomic site for the integration of T7 RNA polymerase and cell-based tetracycline-controlled protein *** findings provide promising advancements in bioproduction technologies,offering flexibility to switch between cell-free and cell-based protein production as needed.

关键词： Inducible expression CHO cells Cell-free protein synthesis CRISPR T7 RNA polymerase eIF2 Rosa26

Neutrophil extracellular traps in the intestinal mucosa of Eimeria-infected animals

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Asian Pacific Journal of Tropical Biomedicine 2016年第4期6卷 301-307页

作者： Tamara Munoz-Caro Liliana Machado Ribeiro da Silva Zaida Renteria-Solis anja Taubert Carlos Hermosilla Institute of Parasitology Justus Liebig University Giessen

Objective:To investigate the presence of neutrophil extracellular traps(NETs) in vivo by analysing intestinal sections from experimentally Eimeria bovis-and naturally Eimeria arloingi-infected ***:Intestinal samples of Eimeria arloingi-and Eimeria bovis-infected animals were analysed by using immunohistochemical and fluorescence approach by using monoclonal ***:Classical NET components were confirmed by co-localization of extracellular DNA being decorated with neutrophil elastase and histones in Eimeria-infected tissue ***,extrusion of NETs was exclusively detected in intestinal polymorphonuclear neutrophils infiltrating Eimeria-infected *** vivo NETs were either found in close proximity or in direct contact to different Eimeria stages suggesting a stage-independent *** were also found within the gut lumen driven by polymorphonuclear neutrophils that were contacting released ***:We postulate that NETs might play an important role in innate defence reactions in coccidiosis therefore significantly altering the outcome of infection.

关键词： Eimeria bovis Eimeria arloingi Neutrophil extracellular traps Apicomplexa Coccidiosis

Real-time molecular imaging of near-surface tissue using Raman spectroscopy

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Light(Science & Applications) 2022年第5期11卷 820-831页

作者： Wei Yang Florian Knorr Ines Latka Matthias Vogt Gunther O.hofmann Jürgen Popp Iwan W.Schie Leibniz Institute of Photonic Technology Jena Albert-Einstein-Straße 907745 JenaGermany Department of Trauma Hand and Reconstructive SurgeryUniversity Hospital JenaAm Klinikum 107747 JenaGermany Institute of Physical Chemistry and Abbe Center of Photonics Friedrich-Schiller University JenaHelmholtzweg 407743 JenaGermany Department of Medical Engineering and Biotechnology University of Applied Sciences-JenaCarlZeiss-Promenade 207745 JenaGermany

The steady progress in medical diagnosis and treatment of diseases largely hinges on the steady development and improvement of modern imaging *** spectroscopy has attracted increasing attention for clinical applications as it is label-free,non-invasive,and delivers molecular fingerprinting information of a *** combination with fiber optic probes,it also allows easy access to different body parts of a ***,image acquisition with fiber optic probes is currently not ***,we introduce a fiber optic probe-based Raman imaging system for the real-time molecular virtual reality data visualization of chemical boundaries on a computer screen and the physical *** approach is developed around a computer vision-based positional tracking system in conjunction with photometric stereo and augmented and mixed chemical reality,enabling molecular imaging and direct visualization of molecular boundaries of three-dimensional *** proposed approach achieves a spatial resolution of 0.5 mm in the transverse plane and a topology resolution of 0.6 mm,with a spectral sampling frequency of 10 Hz,and can be used to image large tissue areas in a few minutes,making it highly suitable for clinical tissueboundary demarcation.A variety of applications on biological samples,i.e.,distribution of pharmaceutical compounds,brain-tumor phantom,and various types of sarcoma have been characterized,showing that the system enables rapid and intuitive assessment of molecular boundaries.

关键词： diagnosis enable intuitive

Transport Logistics in Pollen Tubes

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Molecular Plant 2013年第4期6卷 1037-1052页

作者： Youssef Chebli Jens Kroeger anja Geitmann Departement de sciences biologiques Institut de recherche en biologie vegetale Universite de Montreal Montreal Quebec Canada Raymor Industries Boisbriand Quebec Canada

Cellular organelles move within the cellular volume and the effect of the resulting drag forces on the liquid causes bulk movement in the cytosol. The movement of both organelles and cytosol leads to an overall motion pattern called cytoplasmic streaming or cyclosis. This streaming enables the active and passive transport of molecules and orga- nelles between cellular compartments. Furthermore, the fusion and budding of vesicles with and from the plasma mem- brane （exo/endocytosis） allow for transport of material between the inside and the outside of the cell. In the pollen tube, cytoplasmic streaming and exo/endocytosis are very active and fulfill several different functions. In this review, we focus on the logistics of intracellular motion and transport processes as well as their biophysical underpinnings. We discuss various modeling attempts that have been performed to understand both long-distance shuttling and short-distance targeting of organelles. We show how the combination of mechanical and mathematical modeling with cell biological approaches has contributed to our understanding of intracellular transport logistics.

关键词： transport processes/kinetics cell expansion cytoskeleton dynamics protein traffic and secretion pollendevelopment.

3D printing of patient-specific implants for osteochondral defects: workflow for an MRI-guided zonal design

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Bio-Design and Manufacturing 2021年第4期4卷 818-832页

作者： David Kilian Philipp Sembdner Henriette Bretschneider Tilman Ahlfeld Lydia Mika Jörg Lützner Stefan Holtzhausen anja Lode Ralph Stelzer Michael Gelinsky Centre for Translational Bone Joint and Soft Tissue ResearchUniversity Hospital Carl Gustav Carus and Faculty of MedicineTechnische Universität DresdenDresdenGermany Institute of Machine Elements and Machine Design Faculty of Mechanical EngineeringTechnische Universität DresdenDresdenGermany University Center of Orthopaedic Trauma and Plastic SurgeryUniversity Hospital Carl Gustav CarusTechnische Universität DresdenDresdenGermany

Magnetic resonance imaging(MRI)is a common clinical practice to visualize defects and to distinguish different tissue types and pathologies in the human *** far,MRI data have not been used to model and generate a patient-specific design of multilayered tissue substitutes in the case of interfacial *** orthopedic cases that require highly individual surgical treatment,implant fabrication by additive manufacturing holds great ***-based techniques like 3D plot-ting allow the spatially defined application of several materials,as well as implementation of bioprinting *** the example of a typical multi-zonal osteochondral defect in an osteochondritis dissecans(OCD)patient,this study aimed to close the technological gap between MRI analysis and the additive manufacturing process of an implant based on dif-ferent biomaterial inks.A workflow was developed which covers the processing steps of MRI-based defect identification,segmentation,modeling,implant design adjustment,and implant generation.A model implant was fabricated based on two biomaterial inks with clinically relevant properties that would allow for bioprinting,the direct embedding of a patient’s own cells in the printing *** demonstrated by the geometric compatibility of the designed and fabricated model implant in a stereolithography(SLA)model of lesioned femoral condyles,a novel versatile CAD/CAM workflow was successfully established that opens up new perspectives for the treatment of multi-zonal(osteochondral)defects.

关键词： Additive manufacturing(AM) Magnetic resonance imaging(MRI) Computer-aided design(CAD) Osteochondritis dissecans(OCD) Bone cement Hydrogel

Inflammatory myofibroblastic tumor of the liver:A case report and review of literature

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World Journal of Hepatology 2020年第4期12卷 170-183页

作者： Alexandra Filips Martin H Maurer Matteo Montani Guido Beldi anja Lachenmayer Department of Visceral Surgery and Medicine InselspitalBern University HospitalUniversity of BernBern 3010Switzerland Department of Radiology InselspitalBern University HospitalUniversity of BernBern 3010Switzerland Institute of Pathology InselspitalUniversity HospitalUniversity BernBern 3010Switzerland

BACKGROUND Inflammatory myofibroblastic tumors of the liver(IMTL)are extremely rare neoplasms and very little is known about their clinical presentation,pathogenesis,and biological *** to their absolute rarity,it is almost impossible to obtain a definite diagnosis without histological *** of their intermediate biological behavior with the risk for local recurrence and metastases,surgical resection is recommend whenever IMTL is *** SUMMARY We herein present a case of an otherwise healthy 32-year-old woman who presented with intermittent fever,unclear anemia,malaise and right flank pain 4 mo *** liver mass in segment IVa/b was highly FDG avid in the positron emission tomography-computed *** resection was performed achieving a negative resection margin and an immediate resolution of all clinical *** analysis diagnosed the rare finding of an inflammatory myofibroblastic tumor of the liver and revealed cytoplasmic anaplastic lymphoma kinase expression by *** months follow-up magnetic resonance imaging showed no recurrence and no metastases in the fully recovered *** IMTLs are extremely rare and difficult to *** to their intermediate biological behavior,surgical resection should be perform whenever feasible and patients should be followed-up in order to detect recurrence and metastasis as early as possible.

关键词： Inflammatory myofibroblastic tumor Hepatic Inflammatory Anaplastic lymphoma kinase-expression Case report Review

Hydrogeochemical and multi-tracer investigations of arsenic-affected aquifers in semi-arid West Africa

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Geoscience Frontiers 2019年第5期10卷 1685-1699页

作者： anja Bretzler Lucien Stolze Julien Nikiema Franck Lalanne Elaheh Ghadiri Matthias S. Brennwald Massimo Rolle Mario Schirmer Eawag Swiss Federal Institute of Aquatic Science and TechnologyDübendorfSwitzerland Centre d’Hydrogéologie et de Géothermie (CHYN) Université de NeuchatelSwitzerland Technical University of Denmark (DTU) LyngbyDenmark Université Ouaga I Pr. Joseph KI-ZERBO OuagadougouBurkina Faso Institut International d’Ingénierie de l’Eau et de l’Environnement (2iE) OuagadougouBurkina Faso ETH Zürich Department of Environmental System SciencesZürichSwitzerland

The semi-arid Sahel regions ofWest Africa rely heavily on groundwater from shallow to moderately deep (10 μg/L) stemming from the oxidation of sulphide minerals (pyrite,arsenopyrite) in mineralised *** aquifers are still little investigated,especially concerning groundwater residence times and the influence of the annual monsoon season on groundwater *** gain insights on the temporal aspects of As contamination,we have used isotope tracers (noble gases,3H,stable water isotopes (2H,18O)) and performed hydrochemical analyses on groundwater abstracted from tube wells and dug wells in a small study area in southwestern Burkina *** revealed a great variability in groundwater properties (*** conditions,As concentrations,water level,residence time) over spatial scales of only a few hundred metres,characteristic of the highly heterogeneous fractured *** As levels are found in oxic groundwater of circum-neutral pH and show little relation with any of the measured *** concentrations are relatively stable over the course of the year,with little effect seen by the *** residence time does not seem to have an influence on As concentrations,as elevated As can be found both in groundwater with short (10^3 a) residence times as indicated by 3He/4He ratios spanning three orders of *** results support the hypothesis that the proximity to mineralised zones is the most crucial factor controlling As concentrations in the observed redox/pH *** existence of very old water portions with residence times >10^3 years already at depths of <50 m *** a new finding for the shallow fractured bedrock aquifers of Burkina Faso,suggesting that overexploitation of these relatively low-yielding aquifers may be an issue in the

关键词： Arsenic Groundwater chemistry West Africa Fractured aquifers Residence time Noble gases

蒙特卡洛方法用于城镇5G基站电磁暴露安全评估

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电波科学学报 2023年第5期38卷 903-910页

作者：蒋廷勇 anja K SKRIVERVIK 西北核技术研究所西安710069 瑞士洛桑联邦理工大学洛桑CH1015

电磁暴露安全是当前5G通信在大规模商业化推广过程中需高度重视的问题.大规模多输入多输出(multiple-input multiple-output,MIMO)和天线3D波束成形等技术在现有5G基站中已得到成熟应用,但以往传统确定性方法在评估该类基站时过于保守,... 详细信息

电磁暴露安全是当前5G通信在大规模商业化推广过程中需高度重视的问题.大规模多输入多输出(multiple-input multiple-output,MIMO)和天线3D波束成形等技术在现有5G基站中已得到成熟应用,但以往传统确定性方法在评估该类基站时过于保守,很难得到准确可信的基站电磁暴露评估结果.本文在以往基于蒙特卡洛(Monte Carlo,MC)统计方法的5G基站电磁暴露评估模型基础上,进一步模拟构建了城镇基站小区的三维用户场景,采用电磁暴露相对因子作为衡量指标实现了更真实用户场景下的5G基站电磁暴露水平评估.结果表明,在文中给定的两类基站配置参数下,5G基站小区在最坏情况下电磁暴露相对因子计算结果最大值仅有保守确定性方法给出的电磁暴露评估结果的25%左右,说明5G基站引入大规模MIMO和波束成形技术后不仅改善了用户通信质量,同时也使覆盖区域内电磁暴露量值分布更为均匀,得到了比传统基站更低的电磁暴露水平.

关键词： 5G基站电磁暴露蒙特卡洛(MC)方法统计方法电磁暴露相对因子