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In vitro evaluation of anti-acetylcholinesterase and free radical scavenging potential of leaf extracts of some selected medicinal plants

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Asian Pacific Journal of Tropical Biomedicine 2019年第2期9卷 60-65页

作者： Annie Jessica toppo Sheela Chandra Dhruv Jha Papiya Mitra Mazumder Department of Bio-Engineering Birla Institute of Technology Department of Pharmaceutical Sciences and Technology Birla Institute of Technology

Objective: To evaluate the phytochemical present in various solvent extracts from leaves of Ocimum sanctum(L.), Swertia chirayita(L.), Butea monosperma(Lam.) and Stevia rebaudiana(Bert.) as well as antioxidant and anticholinergic activities employing different in vitro models. Methods: Total phenol content of diethyl ether, chloroform and methanolic extracts obtained from leaves of different medicinal plants was determined by Folin-Ciocalteau's spectrophotometric method. Moreover, antioxidant and anticholinergic studies were conducted by four different in vitro methods which included diphenyl picrylhydrazyl radical scavenging, 2,2-azinobis(3-ethylbezoline-6-sulphonic acid), reducing activity by ferrous reduced antioxidant power and anti-acetylcholinesterase assay, in order to ensure pharmacological potential of the plants. Results: The methanolic leaf extract of Ocimum sanctum showed the highest total phenol content which was(21.13±1.04) GAE/g DW and antioxidant activities compared to other plants with the IC50 value of 40.43 μg/mL in diphenyl picrylhydrazyl radical scavenging assay and 53.5 μg/mL in 2,2-azinobis(3-ethylbezoline-6-sulphonic acid) assay as well as metal ion reduced by(78.22±0.38) TE/g DW in ferrous reduced antioxidant power assay. The inhibition percentage of the anti-acetylcholinesterase assay was(94.22±0.26)%. Conclusions: The results of our current study show that Ocimum sanctum leaf is the most significant source of phytochemicals that possesses antioxidant and anticholinergic properties. However, further investigation on isolation and characterization of active compound which is responsible for the pharmacological potential is needed.

关键词： DPPH assay Antioxidant Free radical Anti-acetylcholinesterase Phenolics content

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现代求婚学问大

空中英语教室（高级版．彭蒙惠英语） 2012年第2期 20-21,42,43页

作者： Greg toppo

一位女士毫无戒备地将钓线抛向湖中，结果钓起一枚闪闪发亮的钻戒，原来是有专业潜水员在水里将钻戒绑在鱼钩上。一对情侣到宜家家居店购物，准新郎突然唱起歌、跳起舞，然后单膝下跪。还有一位自称是“追求刺激者”的男士，为了上演求... 详细信息

一位女士毫无戒备地将钓线抛向湖中，结果钓起一枚闪闪发亮的钻戒，原来是有专业潜水员在水里将钻戒绑在鱼钩上。一对情侣到宜家家居店购物，准新郎突然唱起歌、跳起舞，然后单膝下跪。还有一位自称是“追求刺激者”的男士，为了上演求婚戏码，居然请求两位警察帮忙，在准新娘面前假装以谋杀罪名将他逮捕。

关键词：求婚学问宜家家居潜水员钻戒情侣

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玩游戏能激发孩子阅读经典的兴趣吗?

大学英语 2013年第10期 33-35页

作者： Greg toppo 清朗

20世纪九十年代起小小的Game Boy游戏机便开始占据孩子们的书包,一直以来教师们将视频游戏视作家庭作业的死敌,与书本势不两立。但明年这种情况有望出现转机,纽约一家新兴教育机构将首批推出一千余本书的书目（其中不乏经典）,且让阅读... 详细信息

20世纪九十年代起小小的Game Boy游戏机便开始占据孩子们的书包,一直以来教师们将视频游戏视作家庭作业的死敌,与书本势不两立。但明年这种情况有望出现转机,纽约一家新兴教育机构将首批推出一千余本书的书目（其中不乏经典）,且让阅读围绕游戏进行。Amplify公司研发的学生平板电脑本月开始发运,

关键词：阅读经典游戏机孩子激发九十年代 20世纪家庭作业视频游戏

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谁把图书馆变数码化了？

空中英语教室（高级版．彭蒙惠英语） 2010年第3期 11-12,40页

作者： Greg toppo

库欣私立寄宿中学是很典型的现代化新英格兰寄宿学校。

关键词：数码化图书馆寄宿学校新英格兰现代化

Glycemic Index and Response of a Plant Based Nutritional Supplement and Its Subjective Satiety Following Its Use in Indian Adults

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Food and Nutrition Sciences 2019年第8期10卷 937-946页

作者： Bhoite Rachana Parthasarthy Vijayalakshmi Raman Jeevan Ganesh Viswanathan Gopinath Karthikeyan Parkavi Vasudevan Kavitha Nagamuthu Gayathri Rajagopal Gayathri Shanmugam Shobana Ranjit Mohan Anjana Ranjit Unnikrishnan Vasudevan Sudha Mehta Suyog Archana toppo Panda Acharya Shivani Viswanathan Mohan Dr. Reddy’s Laboratories Hyderabad India Department of Foods Nutrition & Dietetics Research Madras Diabetes Research Foundation Chennai India Department of Diabetology Dr. Mohan’s Diabetes Specialities Centre Chennai India

Background: Diet plays a vital role in managing diabetes. Foods with a low glycemic index provide lower postprandial glucose spikes and induce satiety. The objective of this study was to assess the Glycemic index (GI) without milk and Glycemic response (GR) with milk of two different flavours of a plant-based supplement which is high in protein and fibre, along with a subjective assessment of satiety. Methods: Fifteen overweight/obese subjects aged 18 - 45 years were recruited. After overnight fasting, blood samples were drawn at 5 mins before food consumption (-5), 0, 15, 30, 45, 60, 90 and 120 minutes. Participants underwent 3 days of reference food testing and 1 day of test food in random order with 2 days of wash-out period. The GI was assessed using a validated protocol recognized by FAO/WHO, as well as the guidelines by the International Dietary Carbohydrate Task Force for GI Methodology. The satiety index was measured using the Visual Analog Scale (VAS). The dietary intake of the subjects was measured by 24-hour dietary recall. The Incremental Area Under the Curve (IAUC) was calculated using the trapezoid rule. Results: Both the flavours of the supplement had low GI & GR. The GI and GR of Flavour 1 were 27.3 ± 4.8 & 16.4 ± 2.6 (Mean ± SEM) respectively. For Flavour 2 the GI and GR were 36.7 ± 4.4 & 25.7 ± 2.3 (Mean ± SEM). For Flavour 1, 60% and for flavour 2 66.7% of subjects reported feeling hungry only after 3 hours, showing good satiety. Conclusion: The plant-based high fibre high protein supplement in both flavours showed a low glycemic index and hence may be useful to include in the diets to reduce the postprandial glycemic response and could improve satiety.

关键词： Glycemic Index High Protein High Fiber Satiety Visual Analog Scale

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