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Differential gene expression profile evaluation between uterine leiomyoma and leiomyosarcoma using a machine learning approach

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Gynecology and Obstetrics Clinical Medicine 2023年第3期3卷 154-162页

作者： Sonal Upadhyay Ravi Bhushan anima tripathi Lavina Chaubey Amita Diwakar Pawan K.Dubey Centre for Genetic Disorders Institute of ScienceBanaras Hindu UniversityVaranasi221005Uttar PradeshIndia Department of Zoology MMVBanaras Hindu UniversityVaranasi221005Uttar PradeshIndia Department of Obstetrics and Gynecology Institute of Medical ScienceBanaras Hindu UniversityVaranasi221005Uttar PradeshIndia

Objective The objective of this study is to differentiate between uterine leiomyomas(ULM)and uterine leiomyosarcomas(ULMS)by conducting molecular differential analysis and identifying potential prognostic biomarkers for *** The microarray datasets(GSEID:GSE64763 and GSE185543)were retrieved from the Gene Expression Omnibus *** preprocessing and differential gene expressions(DEGs)analysis were *** DEGs were further intersected to find the common DEGs in ULM and ULMS and further validation of selected DEGs were ***,a machine learning classifier was also applied in the selection of ***-protein interaction network based upon STRING v 10.5,was ***,Gene Ontology(GO)and KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway enrichment analyses were also performed to dissect possible functions and *** A total of 50 significant DEGs for ULM while 321 DEGs for ULMS have been identified with their official gene *** ULM and ULMS,a total of 14 common DEGs were identified of which 8 were up-regulated while 6 were *** DEGs of(GSE185543)were also analyzed and the significant genes were retrieved common in both datasets for further *** a machine learning approach,10 feature genes were *** the expression profiles of these genes,a sequential minimal optimization(SMO)prediction model was built on the training set,and it accurately and reliably classified features expression in ULM and ULMS in the independent test ***,Co-Enrichment analysis was also *** The study identified several DEGs,including ZNF365,EPYC,COL11A1,SHOX2,MMP13,TNN,GPM6A,and GATA2,through cross-validation,machine learning classifier,and Co-Enrichment *** candidate disease genes may provide valuable insight into the underlying mechanisms and could be used as potential diagnostic biomarkers for ULM and ***,further validation of the

关键词： sarcoma approach symbol

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Clomiphene Citrate Induces ROS-Mediated Apoptosis in Mammalian Oocytes

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Open Journal of Apoptosis 2014年第3期3卷 52-58页

作者： Shail K. Chaube Tulsidas G. Shrivastav Shilpa Prasad Meenakshi Tiwari anima tripathi Ashutosh N. Pandey Karuppanan V. Premkumar Cell Physiology Laboratory Biochemistry Unit Department of Zoology Banaras Hindu University Varanasi-221005 India Department of Reproductive Biomedicine National Institute of Health and Family Welfare New Delhi-110067 India

The clomiphene citrate (CC), a nonsteroidal triphenylethylene compound, is a first line of medicine used for the induction of ovulation in anovulatory women worldwide. In spite of high ovulation induction with the use of CC, the pregnancy rate is much lower. Such a discrepancy could be due to the peripheral anti-estrogenic effect of CC, particularly at the level of ovary, endometrium and cervical mucus. CC induces ovulation by binding to the estrogen receptors and generates hypoestrogrnic state in hypothalamus leading to release of pituitary gonadotropins. CC may have a direct effect at the level of ovary but the molecular mechanism remains unclear. animal studies suggest that the CC induces apoptosis in granulosa cells and results hypoestrogenic state in the ovary. Reduced estradiol 17β level in the ovary affects development and maturation of oocyte leading to oocyte apoptosis. Further, CC increases hydrogen peroxide (H2O2) level and thereby bax protein expression and DNA fragmentation in cumulus-granulosa cells as well as in oocytes. The exogenous supplementation of either estradiol 17β or melatonin reduces H2O2 level in ovary, delays meiotic cell cycle progression in oocyte and protects oocyte apoptosis. Hence, supplementation of estradiol 17β or melatonin along with CC could be beneficial to protect granulosa cell as well as oocyte apoptosis and inhibit deterioration of oocyte quality. Thus, maintenance of oocyte quality may overcome the adverse effect caused due to CC treatment during infertility management.

关键词： Clomiphene Citrate Ovulation Induction ROS Generation Apoptosis Oocyte Quality

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