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Micro Flow-Through Thermocycler with Simple Meandering Channel with Symmetric Temperature Zones for Disposable PCR-Devices in Microscope Slide Format

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Journal of Bionic Engineering 2008年第4期5卷 291-298页

作者： anett reichert Jana Felbel Mark Kielpinski Matthias Urban Benno Steinbrecht Thomas Henkel Microfluidics Department Institute of Photonic Technology Arvato Digital Services Manufacturing EMEA

Chip-based flow-through PCR implements the PCR as a continuous process for nucleic acid analytics. The sample is transported in a winding channel through temperature zones required for denaturation, annealing and extension. Main fields of application are the monitoring of continuous processes for rapid identification of contaminants and quality control as well as high throughput screening of cells or microorganisms. A modular arrangement with five heating zones for flow-through PCR is discussed and evaluated. The special heater arrangement allows the implementation of up to 40 cycles on the footprint of a microscope slide, which is placed on top ofa 5 zones heating plate. Liquid/liquid two phase flow of PCR reaction mixture and mineral oil have been applied to create a segmented flow process scheme. In that way, the developed system may provide flow-through PCR as a unit operation for the droplet based microfluidics platform. The single use of disposable devices is commonly preferred due to the sensitivity of the PCR process to contaminations. All-glass microfluidic chips and disposable chip devices, made from polycarbonate as a replication with identically geometry, have been fabricated and tested. For the first time, microchannel geometries with nearly circular profile developed by all-glass technology have been transferred to mass fabrication by injection compression molding. Both devices have been successfully applied for the detection of the tumor suppressor gene p53. Although product yield and selectivity of the amplification process do not depend on the chip material, a well defined, reliable segmented flow regime could only be realized in the all-glass chip.

关键词： lab-on-a-chip PCR microfluidics two-phase-flow injection molding point-of-care biomedical

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An Update on Xylan Synthesis

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Molecular Plant 2012年第4期5卷 769-771页

作者： anett Doering Rahul Lathe Staffan Persson Max-Planck-Institute for Molecular Plant Physiology Am Muehlenberg 1 14476 Potsdam Germany

Plant cell walls are complex structural frameworks that affect cell shape and differentiation, and that protect the plant cell against environmental conditions （Scheller and Ulvskov, 2010）. One of the major componen... 详细信息

关键词： GLYCOSYL TRANSFERASES BIOSYNTHESIS GENES PROTEINS MEMBERS IRX15 GT43

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Chinese Language Learning Strategies Characteristic of Language Learners

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History Research 2015年第1期5卷 61-76页

作者： anett Kozjek-Gulyás Pázmány Péter Catholic University Hungary

The present paper analyzes the strategic choices for learning Chinese by 300 Hungarian university students. The survey summarizes the most often used Chinese language learning strategies and verbal language learning strategies of Hungarian language learners one by one. With the help of the method of multidimensional scaling, the survey got to the result that the preference of the general language learning strategies is characterized by a strong opposition. In the case of the choice and use of three strategies--the social, the meta-cognitive and the cognitive strategies, language learners prefer a combined choice or non-choice. While in the use of the emotional, compensation and memorization strategies, a strong opposition emerges. Those using memorization strategies less prefer the emotional and the compensation strategies. This is true vice versa as well, those using emotional strategies, use memorization and compensation strategies less often. While the relation of the general language learning strategies was characterized by a certain opposition, in the use of the verbal strategies a strict continuity could be detected. Hungarian language learners consider the use of almost all verbal strategies with few exceptions to be successful in their communication. The results of the Variables path analysis with Partial Least-Squares Estimation （LVPLS） model revealed all those connections that structurally exist among the three exogen background latent variables （language level, language learner style and the ascriptive background） and the two endogen latent variables （the language learning strategies and the verbal strategies）. All of these latent variables measured with several manifest variables of the second exogen background variables, the language learning style showed the biggest affect to strategy use, this variable influences the successfulness of the learning of Chinese most strongly.

关键词： Chinese language language learning strategies language learning styles verbal language learning

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Does higher ploidy level increase the risk of invasion?A case study with two geo-cytotypes of Solidago gigantea Aiton(Asteraceae)

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Journal of Plant Ecology 2018年第2期11卷 317-327页

作者： David U.Nagy Szilvia Stranczinger Aliz Godi anett Weisz Christoph Rosche Jan Suda Mark Mariano Robert W.Pal Department of Ecology Institute of BiologyFaculty of SciencesUniversity of PécsH-7624 PécsIfjúság u.6Hungary Department of Botany Institute of BiologyFaculty of SciencesUniversity of PécsH-7624 PécsIfjúság u.6Hungary Working Group:Plant Ecology Institute of Biology/Geobotany and Botanical GardenMartin Luther University Halle-WittenbergAm Kirchtor 1D-06108 HalleGermany Department of Botany Faculty of ScienceCharles University in PragueBenátská2CZ-12801 PragueCzech Republic and Institute of BotanyThe Czech Academy of SciencesZámek 1CZ-25243 PruhoniceCzech Republic Department of Biological Sciences Montana TechMontana Tech of the University of Montana1300 W Park StMT 59701 ButteUSA

Aims Understanding the role of genetics in biological invasions has become an important aspect for modern plant *** studies suggest that increased ploidy level benefits the success of an invasive species,but the basis for this phenomenon is not fully *** its native,North American range,Solidago gigantea has three geo-cytotypes comprising di-,tetra-and hexa-ploid populations,while in Europe,where it is highly invasive,*** stands are composed primarily of tetraploid *** study investigates whether North American hexaploids can induce a greater risk of invasion,due to their higher perfor-mance in a non-native range,as compared to the existing tetra-ploids of that *** We performed greenhouse and common garden experiments along with microsatellite analyses to test whether differences in chromo-some number and origin of the species mean superior fitness in the introduced *** Findings Genetic diversity was significantly higher in the native hexaploid popula-tions(A_(R)=6.04;H_(e)=0.7794),rather than the non-native tetraploid pop-ulations(A_(R)=4.83;H_(e)=0.6869).Furthermore,differentiation between geo-cytotypes was moderate(ρST=0.1838),which was also confirmed by their clear segregation in principal component analysis and structure analyses,proving their different genetic *** contrast to genetic diversity,the non-native tetraploid geo-cytotype performed better in the common garden experiment,implying that higher genetic diversity does not always mean better *** results suggest that native hexa-ploids do not present a greater risk,as assessed by their performance in the introduced range,when compared to the non-native tetraploids,as was suggested by previous ***,their introduction is still undesirable due to their different genetic structure,which,through hybridization,could give a new drive to the invasion of ***.

关键词： EiCA plant invasion microsatellite ecotype polyploidy

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Characterization of the Burning Behaviour of Plastics by a New Method

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Open Journal of Polymer Chemistry 2012年第2期2卷 86-90页

作者： Claus Vogel anett Mueller Dieter Lehmann Frank Taeger Leibniz Institute of Polymer Research Dresden Dresden Germany

A method is described, that allows a quick and simple testing of the burning behaviour of plastics. It takes into account ignition time as well as afterflame time;properties that are characteristic for the burning behaviour of a particular plastic material. The procedure is easy to perform, it does neither require injection-moulded samples nor expensive equipment. The method provides a classification of the burning behaviour. It is especially suitable for a screening of plastic materials complementing established methods like UL-94, GWFI and LOI, and may find application in the development of flame retardants.

关键词： Plastics Burning Behaviour Fire Protection Flammability

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One in four patients with gastrointestinal bleeding develops shock or hemodynamic instability:A systematic review and meta-analysis

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World Journal of Gastroenterology 2023年第28期29卷 4466-4480页

作者： Mahmoud Obeidat Brigitta Teutsch anett Rancz Edina Tari Katalin Márta Dániel Sándor Veres Nóra Hosszúfalusi Emese Mihály Péter Hegyi Bálint Erőss Centre for Translational Medicine Semmelweis UniversityBudapest 1085Hungary Institute for Translational Medicine Medical SchoolUniversity of PécsPécs 7623Hungary Department of Internal Medicine and Hematology Semmelweis UniversityFaculty of MedicineBudapest 1085Hungary Institute of Pancreatic Diseases Semmelweis UniversityBudapest 1083Hungary Department of Biophysics and Radiation Biology Semmelweis UniversityBudapest 1085Hungary

BACKGROUND Hemodynamic instability and shock are associated with untoward outcomes in gastrointestinal ***,there are no studies in the existing literature on the proportion of patients who developed these outcomes after gastrointestinal *** To determine the pooled event rates in the available literature and specify them based on the bleeding *** The protocol was registered on PROSPERO in advance(CRD42021283258).A systematic search was performed in three databases(PubMed,EMBASE,and CENTRAL)on 14^(th) October *** proportions with 95%CI were calculated with a random-effects model.A subgroup analysis was carried out based on the time of assessment(on admission or during hospital stay).Heterogeneity was assessed by Higgins and Thompson’s I^(2) *** Joanna Briggs Institute Prevalence Critical Appraisal Tool was used for the risk of bias *** Reference Citation Analysis(https://***/)tool was applied to obtain the latest highlight *** We identified 11589 records,of which 220 studies were eligible for data *** overall proportion of shock and hemodynamic instability in general gastrointestinal bleeding patients was 0.25(95%CI:0.17-0.36,I^(2)=100%).In non-variceal bleeding,the proportion was 0.22(95%CI:0.14-0.31,I^(2)=100%),whereas it was 0.25(95%CI:0.19-0.32,I^(2)=100%)in variceal *** proportion of patients with colonic diverticular bleeding who developed shock or hemodynamic instability was 0.12(95%CI:0.06-0.22,I^(2)=90%).The risk of bias was low,and heterogeneity was high in all *** One in five,one in four,and one in eight patients develops shock or hemodynamic instability on admission or during hospitalization in the case of non-variceal,variceal,and colonic diverticular bleeding,respectively.

关键词： Gastrointestinal bleeding Hemodynamic instability Shock Meta-analysis Statistics Review

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匈牙利中学汉语作为第二外语毕业考试系统初探

国际汉语教育

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国际汉语教育 2013年第1期 99-110+201页

作者： Kozjek-Gulyás anett 匈牙利天主教大学匈牙利国家考试中心匈牙利中学汉谱毕业考试委员会

随着匈牙利报名参加汉语第二外语课程的学生数量的不断增长,匈牙利中学汉语毕业考试系统的制定迫在眉睫。本文作为对匈牙利中学汉语毕业考试系统的初探,首先介绍匈牙利中学毕业考试改革成果并对比分析三种语言测试系统,然后以《新实用... 详细信息

随着匈牙利报名参加汉语第二外语课程的学生数量的不断增长,匈牙利中学汉语毕业考试系统的制定迫在眉睫。本文作为对匈牙利中学汉语毕业考试系统的初探,首先介绍匈牙利中学毕业考试改革成果并对比分析三种语言测试系统,然后以《新实用汉语课本》为例,分析汉语作为第二外语的中学毕业考试系统,希望提高匈牙利汉语教学的地位,促进匈牙利汉语教学改革。

关键词：匈牙利中学第二外语毕业考试汉语考试语言测试

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脑瘤诱发的组织变化和皮层脑电图分析(英文)

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内蒙古大学学报（自然科学版） 2007年第4期38卷 390-402页

作者： Járdánházy anett Járdánházy Tamás LUO Liao-fu Molnár Joseph 匈牙利塞格特大学神经学系塞格特H-6720 内蒙古大学理工学院理论物理实验室呼和浩特010021 匈牙利塞格特大学医学微生物学和免疫微生物学学院塞格特H-6720

健康组织和肿瘤组织具有不同的代谢,结构和热力学特性.脑瘤的代谢和结构无序性导致其有较高的熵产生.两种组织不同的熵产生决定了熵由癌流向健康组织.脑瘤和正常细胞在熵和能量方面的差异诱发皮层不同的脑电信号.研究了28个脑瘤患者的... 详细信息

健康组织和肿瘤组织具有不同的代谢,结构和热力学特性.脑瘤的代谢和结构无序性导致其有较高的熵产生.两种组织不同的熵产生决定了熵由癌流向健康组织.脑瘤和正常细胞在熵和能量方面的差异诱发皮层不同的脑电信号.研究了28个脑瘤患者的脑电图并和健康人进行比较,观察到脑瘤患者脑电图频率的降低.多数情况中慢a lpha和theta波出现在肿瘤投影区,此可能与其高熵产生和向外的熵流有关.研究认为,脑瘤的持续高熵产生和热耗散及其它物理效应使得皮层电产生慢化,从而使肿瘤及其邻近的脑组织显示出频率较低的电活性.

关键词：脑瘤熵皮层电产生脑电图非线性参数关联维

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Fast-Constructed Marine and Port Structures

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Journal of Shipping and Ocean Engineering 2019年第1期9卷 8-13页

作者： Doubrovsky Michael Poizner Mikhail Oganesyan Virginia Oganesyan anett Odessa National Maritime University Odessa 65029 Ukraine State R&D Institute of Marine Transport Odessa 65058 Ukraine Ukrainian Sea Ports Authority Odessa 65026 Ukraine

In order to provide short construction period and to avoid bulky technologies in maritime conditions some rational structures and installation methods are worked out. These methods may be applied for erection of temporary (seasonable) near-shore constructions;they are comparatively cheap and do not involve heavy construction equipment and mechanisms.

关键词： Temporary berth structure shore protection maritime construction

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