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基于DArT-Seq SNP的高密度遗传图谱的构建及野生型和栽培型西瓜杂交遗传群体偏分离基因组区域的鉴定

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中国瓜菜 2019年第8期32卷 172-173页

作者：任润生羊杏平(编译) Rumiana Ray 李苹芳徐锦华张曼刘广姚协丰 andrzej kilian 江苏省农业科学院蔬菜作物研究所·华东地区国家农业科技创新中心园艺作物遗传改良实验室南京210014 华东地区国家农业科技创新中心园艺作物遗传改良实验室南京210014 Division of Plant and Crop Sciences School of BiosciencesUniversity of Not tinghamSutton Bonington Campus Loughborough LEI2 5RD UK DArT P/L PO Box 7141 Yarralumla Canberra Australia

目的与意义:西瓜[Citrullus lanatus (Thunb.) *** Nakai]是重要的蔬菜作物,在全世界范围内都广泛种植.构建高密度的西瓜遗传图谱和不断利用新开发的标记构建饱和遗传图谱仍然是目前西瓜基因组学研究的重要内容之一.本项研究主要以西瓜... 详细信息

目的与意义:西瓜[Citrullus lanatus (Thunb.) *** Nakai]是重要的蔬菜作物,在全世界范围内都广泛种植.构建高密度的西瓜遗传图谱和不断利用新开发的标记构建饱和遗传图谱仍然是目前西瓜基因组学研究的重要内容之一.本项研究主要以西瓜自交系‘K3’和野生西瓜种‘PI189225’为亲本构建F2分离群体,利用以DArTseq为基础的SNP标记构建西瓜高密度遗传连锁图谱.

关键词： SNP标记遗传图谱基因组学西瓜种高密度野生型遗传群体偏分离

基于DArTseq的SNP标记的西瓜核心种质遗传多样性和群体结构分析

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中国瓜菜 2019年第8期32卷 174-175页

作者：任润生羊杏平 Rumiana Ray 徐锦华李苹芳张曼刘广姚协丰 andrzej kilian 江苏省农业科学院蔬菜作物研究所华东地区国家农业科技创新中心园艺作物遗传改良实验室 Division of Plant and Crop Sciences School of BiosciencesUniversity of Nottingham Sutton Bonington Campus Loughborough LE12 5RD UK Diversity Arrays Technology University of Canberra

目的与意义:亲本材料是进行育种工作的基础,遗传基础狭窄导致难以培育出突破性品种,因此亟需加强遗传资源的引进及其农艺性状特征和遗传基础的了解与掌握,对现有优良西瓜品种和育种亲本材料进行遗传多样性研究,可有效减少相似遗传背景... 详细信息

目的与意义:亲本材料是进行育种工作的基础,遗传基础狭窄导致难以培育出突破性品种,因此亟需加强遗传资源的引进及其农艺性状特征和遗传基础的了解与掌握,对现有优良西瓜品种和育种亲本材料进行遗传多样性研究,可有效减少相似遗传背景的组合,减少育种的工作量。同时,随着分子生物学技术的发展,分子标记种类及检测手段日趋完善,利用分子标记技术对植物进行聚类分析,可以快速了解植物的遗传多样性、亲缘关系和品种鉴定等。本研究对37份核心西瓜种质进行遗传多样性分析,确定这些西瓜核心种质材料间的遗传背景差异,为后期育种工作中的亲本组配、等位基因发掘和标记辅助选择等提供参考。

关键词： SNP标记遗传图谱基因组学西瓜种高密度野生型遗传群体偏分离

Model Predictive Control based Motion Cueing Algorithm for Driving Simulator

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Journal of Systems Science and Systems Engineering 2024年第5期33卷 607-626页

作者： Ayesha Hameed Ali Soltani Sharif Abadi andrzej Ordys Institute of Automatic Control and Robotics Warsaw University of TechnologyWarsawPoland Institute of Electronic Systems Warsaw University of TechnologyWarsawPoland

Thanks to the emerging integration of algorithms and simulators, recent Driving Simulators (DS) find enormous potential in applications like advanced driver-assistance devices, analysis of driver’s behaviours, research and development of new vehicles and even for entertainment purposes. Driving simulators have been developed to reduce the cost of field studies, allow more flexible control over circumstances and measurements, and safely present hazardous conditions. The major challenge in a driving simulator is to reproduce realistic motions within hardware constraints. Motion Cueing Algorithm (MCA) guarantees a realistic motion perception in the simulator. However, the complex nature of the human perception system makes MCA implementation challenging. The present research aims to improve the performance of driving simulators by proposing and implementing the MCA algorithm as a control problem. The approach is realized using an actual vehicle model integrated with a detailed model of the human vestibular system, which accurately reproduces the driver’s perception. These perception motion signals are compared with simulated ones. A 2-DOF stabilized platform model is used to test the results from the two proposed control strategies, Proportional Integrator and Derivative (PID) and Model Predictive Control (MPC).

关键词： Driving simulator motion cueing algorithm control theory model predictive control PID

Editor-in-Chief articles of choice and comments at the year-end of 2023

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World Journal of Gastroenterology 2024年第1期30卷 1-8页

作者： andrzej S Tarnawski Department of Gastroenterology Research University of California Irvine and the Veterans Administration Long Beach Healthcare SystemLong BeachCA 90822United States

As the Editor-in-Chief of World Journal of Gastroenterology,every week prior to a new issue’s online publication,I perform a careful review of all encompassed articles,including the title,clinical and/or research importance,originality,novelty,and ratings by the peer *** on this review,I select the papers of choice and suggest pertinent changes(e.g.,in the title)to the Company Editors responsible for *** process,while time-consuming,is very important for assuring the quality of publications and highlighting important articles that Readers may revisit.

关键词： Papers of choice Careful weekly review Suggested changes/revisions Hepatocellular carcinoma Pancreatic cancer Liver cirrhosis Liver injury Gastric cancer Colorectal cancer Inflammatory bowel diseases

Localisation of Loci Involved in Resistance to Diaporthe toxicaand Pleiochaeta setosain White Lupin (Lupinus albusL.)

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Open Journal of Genetics 2014年第3期4卷 210-226页

作者： Rosy Raman Cina Vipin David J. Luckett Raymond B. Cowley Gavin J. Ash John D. I. Harper andrzej kilian Harsh Raman DArT Pty Ltd. (ACT) University of Canberra Canberra Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of Primary Industries) Pugsley Place Wagga Wagga Agricultural Institute Wagga Wagga Australia NSW Department of Primary Industries Agricultural Institute Wagga Wagga Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of Primary Industries) Pugsley Place Wagga Wagga Agricultural Institute Wagga Wagga Australia School of Agricultural & Wine Sciences Charles Sturt University Wagga Wagga Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of Primary Industries) Pugsley Place Wagga Wagga Agricultural Institute Wagga Wagga Australia School of Agricultural & Wine Sciences Charles Sturt University Wagga Wagga Australia Graham Centre for Agricultural Innovation (An Alliance between Charles Sturt University and NSW Department of Primary Industries) Pugsley Place Wagga Wagga Agricultural Institute WaggaWagga Australia NSW Department of Primary Industries Agricultural Institute Wagga Wagga Australia NSW Department of Primary Industries Agricultural Institute Wagga Wagga Australia

L. albus is an annual grain-legume crop mainly grown for high-protein fodder worldwide but also to produce large seeds for human consumption as a snack-food. In order to make genetic gains in grain yield, assessment of the genetic variation in the germplasm and identification of loci associated with agronomic traits are essential. Phomopsis blight (PB) and Pleiochaeta root rot (PRR), caused by the fungal pathogens Diaporthe toxica and, Pleiochaeta setosa respectively, are two major yield-limiting diseases of the L. albus crop. The extent of genetic diversity in 94 accessions of L. albus comprising: Australian and exotic cultivars, advanced breeding lines, and landraces originating from 26 different countries was determined utilizing PCR-based genic, and microarray-based Diversity Arrays Technology (DArT™), markers. All accessions were evaluated for resistance to PB in two plant tissues (leaves and stems) using either sprayed or injected spore inoculum. A subset of 58 accessions was further evaluated for resistance to PRR by growing seedlings in spore-infested potting mix. The combined data of 724 (50 genic- and 674 DArT) markers were used for cluster analysis. A subset of 324 markers with call rate ≥95% and predicted disease scores of different genotypes were used to identify marker loci accounting for phenotypic variation in PB and PRR resistance using linear regression analysis. Several markers showed significant association with PB or PRR resistance at P < 0.05. Our results showed that favourable alleles for PB and PRR resistance are present in the diverse accessions investigated and they will provide valuable materials for lupin breeding.

关键词： L. albus Genetic Diversity Phomopsis Blight Pleiochaeta Root Rot DArT Linear Regression Analysis Genome-Wide Association Analysis

Early response of understory vegetation to the mass dieback of Norway spruce in the European lowland temperate forest

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Forest Ecosystems 2024年第2期11卷 214-225页

作者： Jan Marek Matuszkiewicz andrzej N.Affek Piotr Zaniewski Ewa Kołaczkowska Faculty of Geography and Regional Studies University of WarsawPoland Institute of Geography and Spatial Organization Polish Academy of SciencesPoland Institute of Forest Sciences Warsaw University of Life SciencesPoland

Spruce-dominated forests are commonly exposed to disturbances associated with mass occurrences of bark *** dieback of trees triggers many physical and chemical processes in the ecosystem resulting in rapid changes in the vegetation of the lower forest *** aimed to determine the response of non-tree understory vegetation to the mass dieback of Norway spruce(Picea abies)in the first years after the disturbance caused by the European spruce bark beetle(Ips typographus)*** study area was the Białowieża Biosphere Reserve covering the Polish part of the emblematic Białowieża Forest,in total 597km^(2).The main data source comprised 3,900 phytosociological relevés(combined spring and summer campaigns)collected from 1,300 systematically distributed forest sites in 2016–2018–the peak years of the bark beetle *** found that the understory responded immediately to mass spruce dieback,with the most pronounced changes observed in the year of the disturbance and the subsequent ***-tolerant forest species declined in the initial years following the mass spruce dieback,while hemicryptophytes,therophytes,light-demanding species associated with non-forest seminatural communities,as well as water-demanding forest species,*** acetosella,the most common understory species in the Białowieża Forest,showed a distinct fluctuation pattern,with strong short-term expansion right after spruce dieback,followed by a gradual decline over the next 3–4 years to a cover level 5 percentage points lower than before the ***,our study revealed that mass spruce dieback selectively affects individual herb species,and their responses can be directional and non-directional(fluctuation).Furthermore,we demonstrated that the mass dieback of spruce temporarily increases plant species diversity(α-diversity).

关键词： Forest disturbance Bark beetle outbreak Picea abies Ips typographus Response of understory vegetation Herbaceous species Biaowieza Forest

Recent progress in Ni-rich layered oxides and related cathode materials for Li-ion cells

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International Journal of Minerals,Metallurgy and Materials 2024年第11期31卷 2345-2367页

作者： Boyang Fu Maciej Moździerz andrzej Kulka Konrad Świerczek Faculty of Energy and Fuels AGH University of Krakowal.A.Mickiewicza 3030-059 KrakowPoland AGH Centre of Energy AGH University of Krakowul.Czarnowiejska 3630-054 KrakowPoland

Undoubtedly,the enormous progress observed in recent years in the Ni-rich layered cathode materials has been crucial in terms of pushing boundaries of the Li-ion battery(LIB)*** achieved improvements in the energy density,cyclability,charging speed,reduced costs,as well as safety and stability,already contribute to the wider adoption of LIBs,which extends nowadays beyond mobile electronics,power tools,and electric vehicles,to the new range of applications,including grid storage *** numerous published papers and broad reviews already available on the subject of Ni-rich oxides,this review focuses more on the most recent progress and new ideas presented in the literature *** covered topics include doping and composition optimization,advanced coating,concentration gradient and single crystal materials,as well as innovations concerning new electrolytes and their modification,with the application of Ni-rich cathodes in solid-state batteries also *** cathode materials are briefly mentioned,with the high-entropy approach and zero-strain concept presented as well.A critical overview of the still unresolved issues is given,with perspectives on the further directions of studies and the expected gains provided.

关键词： lithium-ion batteries cathode materials nickel-rich layered oxides recent progress critical issues improvement strategies

Crossover to PDT after the unsuccessful micropulse laser treatment of central serous chorioretinopathy

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Advances in Ophthalmology Practice and Research 2024年第1期4卷 32-38页

作者： Maciej Gawęcki Krzysztof Kicinski andrzej Grzybowski Dobry Wzrok Ophthalmological Clinic 80-822GdanskPoland Department of Ophthalmology Pomeranian Hospitals in Wejherowo84-200WejherowoPoland Institute for Research in Ophthalmology Foundation for Ophthalmology DevelopmentPoznanPoland

Purpose:Subthreshold micropulse laser(SML)and photodynamic therapy(PDT)are among the most effective therapeutic modalities applied to central serous chorioretinopathy(CSCR).This study aimed to evaluate the ef-ficacy and durability of PDT in CSCR cases unresponsive to at least two SML ***:The study included 26 consecutive eyes of 24 patients(21 males and three females)with chronic *** all cases,a lack of reduction in subretinal fluid(SRF)levels was noted after at least two consecutive SML *** parameters of best corrected visual acuity(BCVA)and spectral domain optical coherence tomog-raphy(SD-OCT)were evaluated at baseline and 1,3 and 12 months ***:The mean duration of symptoms in the group was 53.81±39.48 months,the mean age of the patients was 49.26±12.91 years,and the mean subfoveal choroidal thickness(SFCT)was 572.11±116.21 *** resorption of SRF was observed in 21 out of 26 eyes(80.77%)at 1 month and sustained in 18 cases(69.23%)at 12 *** 12 months,in the sustained group,BCVA improved significantly from 0.39±0.18 to 0.19±0.2 logMAR(P=0.01),central subfoveal thickness(CST)reduced from 316.44±75.83 mm to 197.67±22.99 mm(P<0.0001),and SFCT reduced from 579.28 mm to 446.78 mm(P<0.0001).Conclusions:PDT provides the opportunity for the successful treatment of CSCR unresponsive to SML *** are possible even in cases with a long duration of symptoms and significant alterations in retinal ***,PDT should be considered for patients with prominently increased choroidal thickness.

关键词： Photodynamic therapy Subthreshold micropulse laser Central serous chorioretinopathy Sybretinal fluid

Review Integration of smartphone technology and artificial intelligence for advanced ophthalmic care:A systematic review

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Advances in Ophthalmology Practice and Research 2024年第3期4卷 120-127页

作者： Kai Jin Yingyu Li Hongkang Wu Yih Chung Tham Victor Koh Yitian Zhao Ryo Kawasaki andrzej Grzybowski Juan Ye Eye Center The Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang Provincial Key Laboratory of OphthalmologyZhejiang Provincial Clinical Research Center for Eye DiseasesZhejiang Provincial Engineering Institute on Eye DiseasesHangzhouChina Centre for Innovation and Precision Eye Health National University of SingaporeSingapore Department of Ophthalmology National University of SingaporeSingapore Singapore Eye Research Institute Singapore National Eye CentreSingapore Ophthalmology and Visual Science Academic Clinical Program Duke-NUS Medical SchoolSingapore Cixi Institute of Biomedical Engineering Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Ningbo Eye Hospital NingboChina Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Zhejiang Engineering Research Center for Biomedical Materials Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingboChina Division of Public Health Department of Social MedicineGraduate School of MedicineOsaka UniversityOsakaJapan Artificial Intelligence Center for Medical Research and Application Osaka University HospitalOsakaJapan Institute for Research in Ophthalmology Foundation for Ophthalmology DevelopmentPoznanPoland A R T I C L

Background:The convergence of smartphone technology and artificial intelligence(AI)has revolutionized the landscape of ophthalmic care,offering unprecedented opportunities for diagnosis,monitoring,and management of ocular ***,there is a lack of systematic studies on discussing the integration of smart-phone and AI in this *** text:This review includes 52 studies,and explores the integration of smartphones and AI in ophthalmology,delineating its collective impact on screening methodologies,disease detection,telemedicine initiatives,and patient *** collective findings from the curated studies indicate promising performance of the smartphone-based AI screening for various ocular diseases which encompass major retinal diseases,glaucoma,cataract,visual impairment in children and ocular surface ***,the utilization of smartphone-based imaging modalities,coupled with AI algorithms,is able to provide timely,efficient and cost-effective screening for ocular *** modality can also facilitate patient self-monitoring,remote patient monitoring and enhancing accessibility to eye care services,particularly in underserved *** involving data pri-vacy,algorithm validation,regulatory frameworks and issues of trust are still need to be ***,evaluation on real-world implementation is imperative as well,and real-world prospective studies are currently ***:Smartphone ocular imaging merged with AI enables earlier,precise diagnoses,personalized treat-ments,and enhanced service accessibility in eye *** is crucial to navigate ethical and data security challenges while responsibly leveraging these innovations,promising a potential revolution in care access and global eye health equity.

关键词： Artificial intelligence Smartphone imaging Ophthalmology Telemedicine Ocular disease management