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The Triple Gene Block 1 Movement Protein of the ND18 Strain of Barley Stripe Mosaic Virus is an Avirulence Determinant That Elicits Brachypodium distachyon Bd3-1 resistance

The Triple Gene Block 1 Movement Protein of the ND18 Strain ...

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中国植物病理学会2012年学术年会

作者： LEE Mi-Yeon Florien Gorter Brian Y.T Kim Jessica Grindheim Uma Ganesan andrew o.jackson Department of Plant & Microbial Biology University of California-BerkeleyBerkeleyCA 94720USA

Barley stripe mosaic virus(BSMV) North Dakota 18(ND18),Norwich(NW),Beijing (BJ),Xinjiang(XJ),Type and CV21 strains elicit different phenotypic responses on Brachypodium distachyon, a cereal grass model for ***18 is unable to infect the Brachypodium Bd3-1 inbred line,which contains a recently mapped resistance gene designated Bsr1(Cui et *** oNE,2012, 7(6);e38333).Along with ND18,the BJ,XJ,Type and CV21 strains also failed to produce visible symptoms or virus on Bd3-1,so it is possible that Bsr1 mediates resistance against all five BSMV strains. In contrast,NW overcomes Bd3-1 resistance and is accompanied by an intense mosaic phenotype and large amounts of *** of ND18 and NW genomic RNAs,and a series of chimeric exchanges within RNAβdemonstrate that Bd3-1 phenotypes are mediated by a region within the helicase motif of *** of this region reveals that the ND18 and NW strains contain divergent amino acids at positions 390,392 and 404 within this region,and that the inability of ND18 and the BJ,XJ,Type and CV21 strains to elicit fully virulent BSMV infections on Bd3-1 correlates with a threonine(T) at position 392(T).The virulent NW strain contains K residues at both the 390 and 392 positions and a glutamate (E) residue at position *** substitutions to swap the ND18 and NW 404 residues failed to affect the respective disease ***,R390K and T392K single exchanges and a R390K/ T392K double mutant in ND18 RNA TGB1 resulted in virulent *** contrast,incorporation of single K390R or K392T mutations into the NW TGB1 protein background did not affect the susceptible disease phenotype,suggesting that the NW K and K residues compensate for each *** contrast, a K390R/K392T double mutant in the NW TGB1 background failed to develop fully systemic infections on Bd3-1,or accumulate detectable viral RNA,but prominent necrotic streaks radiating from the secondary leaf veins were *** experiments in toto

关键词： Barley stripe mosaic virus Brachypodium distachyon resistance ND18 strain avirulence determinants Triple Gene Block 1(TGB1)

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GFP Expression from A Biologically Active Minireplicon of Sonchus Yellow Net Virus

GFP Expression from A Biologically Active Minireplicon of So...

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中国植物病理学会2011年学术年会

作者： andrew o.jackson Uma Ganesan Manling Shi Jennifer N.Bragg Mi-Yeon Lee Justin Kappel Cole Peters Sharon Marr University of California BerkeleyCA 94720

＜正＞Sonchus yellow net virus（SYNV） is an extensively characterized plant rhabdovirus that replicates in the nucleus and undergoes morphogenesis at the inner nuclear *** minimum infectious unit of SYNV is a ribonucleoprotein core complex,consisting of the nucleocapsid（N） protein,the phosphoprotein（P） and the polymerase（L） protein complexed with the negative-strand genomic（g） *** genetic analysis of plant negative strand viruses,in contrast to animal negative strand

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对二穗短柄草中抗大麦条纹花叶病毒基因的遗传分析

对二穗短柄草中抗大麦条纹花叶病毒基因的遗传分析

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中国植物病理学会2011年学术年会

作者：崔钰 Naxin Huo John P.Vogel David F.Garvin andrew o.jackson 刘志勇中国农业大学 USDA-ARS Western Regional Research CenterAlbanyCAUSA USDA-ARS-PSRU University of MinnesotaSt.PaulMNUSA University of California BerkeleyCAUSA.

＜正＞短柄草（Brachypodium distachyon）是一种广泛分布于温带地区的禾本科植物,基因组较小（272Mb）、染色体少、DNA重复序列少（大约15%）、植株较矮、生育期较短,与小麦族植物一样具有二倍体、四倍体和六倍体,且不需要严格的生长...

＜正＞短柄草（Brachypodium distachyon）是一种广泛分布于温带地区的禾本科植物,基因组较小（272Mb）、染色体少、DNA重复序列少（大约15%）、植株较矮、生育期较短,与小麦族植物一样具有二倍体、四倍体和六倍体,且不需要严格的生长条件和栽培措施,是大麦、小麦等早熟禾谷类理想的模式植物。随着二穗短柄草自交系Bd21基因组测序的完成,农杆菌遗传转化体系的建立和T-DNA插入突变及EMS化学诱变突

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Calcium channels caught in peripheral glia’s tug-of-war on axon regeneration in Drosophila

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Neural Regeneration Research 2025年第2期20卷 475-476页

作者： jackson Powell Tobias Steinschaden Rose Horowitz Yuanquan Song Raymond G.Perelman Center for Cellular and Molecular Therapeutics The Children’s Hospital of PhiladelphiaPhiladelphiaPAUSA The Neuroscience Graduate Group University of PennsylvaniaPhiladelphiaPAUSA Department of Pathology and Laboratory Medicine University of PennsylvaniaPhiladelphiaPAUSA Medical University of Vienna Center for Brain ResearchDepartment of NeuroimmunologyViennaAustria

Neural damage or degeneration is at the crux of many diseases,and treatment of these diseases will require the development of therapeutics to enhance and guide neural *** intrinsic and extrinsic factors dictate a neuron’s ability to regenerate,and the combination of these factors results in the great regenerative capacity of the peripheral nervous system(PNS)and the poor regenerative capacity of the central nervous system(CNS)following *** the core of a neuron’s function is its ability to relay electrochemical signals,and a neuron’s excitability is a key factor in its ability to *** works have focused on the changes in neuronal electrophysiological properties,firing patterns,and ion flux after injury,which differentially activate signaling pathways at the core of *** role of glia in neuron regeneration has long been studied.

关键词： degeneration firing system

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Bioinspired optical antennas: gold plant viruses

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Light(Science & Applications) 2015年第1期4卷 447-453页

作者： SoonGweon Hong Mi Yeon Lee andrew o.jackson Luke P Lee Department of Bioengineering University of California at BerkeleyBerkeleyCA 94720USA Berkeley Sensor and Actuator Center University of California at BerkeleyBerkeleyCA 94720USA Department of Plant&Microbial Biology University of California at BerkeleyBerkeleyCA 94720USA Department of Electrical Engineering and Computer Sciences University of California at BerkeleyBerkeleyCA 94720USA Biophysics Graduate Program University of California at BerkeleyBerkeleyCA 94720USA

The ability to capture the chemical signatures of biomolecules(i.e.,electron-transfer dynamics)in living cells will provide an entirely new perspective on biology and *** can be accomplished using nanoscale optical antennas that can collect,resonate and focus light from outside the cell and emit molecular ***,we describe biologically inspired nanoscale optical antennas that utilize the unique topologies of plant viruses(and thus,are called gold plant viruses)for molecular fingerprint *** electromagnetic calculations for these gold viruses indicate that capsid morphologies permit high amplification of optical scattering energy compared to a smooth *** experimental measurements of various gold viruses based on four different plant viruses,we observe highly enhanced optical cross-sections and the modulation of the resonance wavelength depending on the viral ***,in label-free molecular imaging,we successfully obtain higher sensitivity(by a factor of up to 10^(6))than can be achieved using similar-sized *** virtue of the inherent functionalities of capsids and the plasmonic characteristics of the gold layer,a gold virus-based antenna will enable cellular targeting,imaging and drug delivery.

关键词： molecular sensor nanophotonics optical antenna optical spectroscopy plant virus plasmonics plasmonic resonant energy transfer(PRET) surface enhanced Raman scattering(SERS)

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Virus-Induced Gene Silencing in the Culinary Ginger （Zingiber officinale）： An Effective Mechanism for Down-Regulating Gene Expression in Tropical Monocots

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Molecular Plant 2009年第5期2卷 1084-1094页

作者： Tanya Renner Jennifer Bragg Heather E. Driscoll Juliana Cho andrew o. jackson Chelsea D. Specht Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Present address: Genornics and Gene Discovery USDA ARS PWA WRRC-GGD 800 Buchanan Street Albany CA 94710 USA

Virus-induced gene silencing （VIGS） has been shown to be effective for transient knockdown of gene expression in plants to analyze the effects of specific genes in development and stress-related responses. VlGS is well established for studies of model systems and crops within the Solanaceae, Brassicaceae, Leguminaceae, and Poaceae, but only recently has been applied to plants residing outside these families. Here, we have demonstrated that barley stripe mosaic virus （BSMV） can infect two species within the Zingiberaceae, and that BSMV-VIGS can be applied to specifically down-regulate phytoene desaturase in the culinary ginger Zingiber officinale. These results suggest that extension of BSMV-VlGS to monocots other than cereals has the potential for directed genetic analyses of many important temperate and tropical crop species.

关键词： Barley stripe mosaic virus virus-induced gene silencing VIGS Zingiber officinale Monocot.

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Mechanotransduction mechanisms in central nervous system glia

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Neural Regeneration Research 2023年第5期18卷 1031-1032页

作者： Brenan Cullimore jackson Baumann Christopher N.Rudzitis andrew o.Jo Denisa Kirdajova David Krizaj Department of ophthalmology and Visual Sciences University of Utah School of MedicineSalt Lake CityUTUSA Department of Cellular Neurophysiology Institute of Experimental MedicineCzech Academy of SciencesPragueCzech Republic Department of Bioengineering University of UtahSalt Lake CityUTUSA Department of Neurobiology University of UtahSalt Lake CityUTUSA

Mechanical forces shape the development,function,and survival of every cell within the central nervous system(CNS)but are particularly important for astroglia,a subtype of glial cell that mediates communication betwee... 详细信息

关键词： system mechanisms subtype

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Alpha-adrenoceptor antagonism by Crassostrea gigas oyster extract inhibits noradrenalineinduced vascular contraction in Wistar rats

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Journal of Integrative Medicine 2015年第3期13卷 194-200页

作者： Kylie Connolly Douglas jackson Candice Pullen andrew Fenning School of Medical and Applied Sciences CQUniversity Australia

oBJECTIVE： Crassostrea gigas oyster extract has been reported to have antioxidant, antihypertensive and lipid-lowering properties that may be useful for treating cardiovascular diseases. This study aimed to evaluate the effect of C. gigas oyster extract on cardiovascular function in tissues from healthy rats. METHoDS： Single-cell microelectrode and isolated thoracic aortic organ bath studies were performed on tissues from 8-week-old healthy Wistar rats, using varying concentrations of C. gigas oyster extract. To elucidate a mechanism of action for the oyster’s vasoactive properties, concentration response curves were carried out in the presence of a calcium channel inhibitior（verapamil）, a nitric oxide synthase inhibitor（N G-nitro-L-arginine methyl ester）, a potassium channel inhibitor（4-aminopyridine）, in addition to the α-adrenoceptor inhibitor prazosin. RESULTS： oyster solution at 7 500 mg/m L inhibited noradrenaline-induced contraction in isolated aortic rings. Cardiac electrophysiology results showed that neither concentration of oyster solution was able to significantly reduce action potential duration at all phases of repolarisation in left ventricular papillary muscles from healthy animals. CoNCLUSIoN： When administered to healthy vascular tissue, C. gigas oyster extract inhibits contraction induced by noradrenaline. This effect is likely to be mediated through α-adrenoceptor inhibition, and to a lesser extent, calcium modulating activity.

关键词： Crassostrea hypertension calcium channels receptors,adrenergic rats

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GANana:Unsupervised Domain Adaptation for Volumetric Regression of Fruit

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Plant Phenomics 2021年第1期3卷 326-336页

作者： Zane K.J.Hartley Aaron S.jackson Michael Pound andrew P.French School of Computer Science University of NottinghamNG71BBUK School of Biosciences University of NottinghamLE125RDUK

3D reconstruction of fruit is important as a key component of fruit grading and an important part of many size estimation *** many computer vision challenges,the 3D reconstruction task suffers from a lack of readily available training data in most domains,with methods typically depending on large datasets of high-quality image-model *** this paper,we propose an unsupervised domain-adaptation approach to 3D reconstruction where labelled images only exist in our source synthetic domain,and training is supplemented with different unlabelled datasets from the target real *** approach the problem of 3D reconstruction using volumetric regression and produce a training set of 25,000 pairs of images and volumes using hand-crafted 3D models of bananas rendered in a 3D modelling environment(Blender).Each image is then enhanced by a GAN to more closely match the domain of photographs of real images by introducing a volumetric consistency loss,improving performance of 3D reconstruction on real *** solution harnesses the cost benefits of synthetic data while still maintaining good performance on real world *** focus this work on the task of 3D banana reconstruction from a single image,representing a common task in plant phenotyping,but this approach is general and may be adapted to any 3D reconstruction task including other plant species and organs.

关键词： image render adapted

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Concept of a Pitot Tube Able to Detect Blockage by Ice, Volcanic Ash, Sand and Insects, and to Clear the Tube

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Photonic Sensors 2015年第4期5卷 298-303页

作者： David A. jackson School of Physical Sciences Applied Optics Groups University of Kent UK

A conceptual coaxial Pitot tube （PT） has been developed using fiber optic sensors combined with actuators to monitor and maintain its correct operation under different environmental conditions. Experiments were performed showing that the dynamic and static tubes can be cleared of ice. It was also demonstrated that the dynamic tube could be cleared of dust and sand which was not the case for the static tube in the coaxial configuration. An approach was proposed to overcome this problem involving a conventional configuration where the static tube was operated independently orthogonal to the dynamic tube, and a second set of sensors and actuators was used. Sensors and associated actuators were developed for temperature and intensity for a linear PT. The aim of this work is to propose a solution for a problem that has caused the loss of the lives of many passengers and crew of aircraft. Resources were not available to test a full implementation of a PT incorporating the proposed modifications.

关键词： Pitot tube instrumentation FBG spectrometer pressure temperature optical intensity

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