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BCRP基因在非小细胞肺癌组织和非癌肺组织中的表达及意义

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癌症 2001年第3期20卷 274-278页

作者：毛友生 Austin Doyle Weidong Yang Yuetong Wei Mark j.Krasna Douglas D.ross 中国医学科学院中国协和医科大学肿瘤医院胸外科北京100021 Greenebaum Cancer Center University of Maryland Medical School

目的：进一步研究从人类乳腺癌细胞株 (MCF-7/AdrVp)中克隆出的、对蒽环类抗癌药耐药的 BCRP基因在非癌肺组织及非小细胞肺癌组织中的表达。方法：从术后即刻获得的正常肺组织和有活力的非小细胞肺癌组织新鲜标本中及时提取细胞总 RNA... 详细信息

目的：进一步研究从人类乳腺癌细胞株 (MCF-7/AdrVp)中克隆出的、对蒽环类抗癌药耐药的 BCRP基因在非癌肺组织及非小细胞肺癌组织中的表达。方法：从术后即刻获得的正常肺组织和有活力的非小细胞肺癌组织新鲜标本中及时提取细胞总 RNA，通过 RT-PCR及 PCR法获得并扩增 BCRP基因的 cDNA产物，再经凝胶电泳分离 BCRP基因 cDNA带，然后再通过转膜技术把这些 BCRP基因 cDNA产物转移到杂交膜上，用 Southern blot杂交技术检测 BCRP基因的表达程度。结果：细胞总 RNA分别从 8个只有肺癌组织标本和 12对同时获取的肺癌组织及非癌肺组织标本中成功提取，然后用变性凝胶电泳鉴定提取 RNA的质量。其中 4例标本因术中缺血时间太长，或有坏死炎症或术前放化疗等原因，所提 RNA有降解而放弃进一步的检测。通过 RT-PCR及 PCR方法从 16例可用标本中获得并扩增 BCRP基因的 cDNA产物，再通过转膜及 Southern blot杂交技术，最终发现 BCRP基因在正常肺组织中均有不同程度的表达（ 10/10），而在非小细胞肺癌组织标本中只有一半病人的标本有不同程度的表达 (8/16)。结论： BCRP基因可能是一个在非癌肺组织中常规表达的基因，而在部分病人的肺癌组织中可能此基因有缺失或被抑制。因此。

关键词： BCRP基因多药耐药性非小细胞肺癌基因表达

维普期刊数据库

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往复式发动机轴承轴心轨迹

柴油机设计与制造 1989年第1期 106-129页

作者： ross,j.M 汪胜波

利用饲草生产模型的气象记录预报放牧地的饲料生产

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草原与草坪 1985年第2期 2-3页

作者： j.ross Wight 冯强

文章论述了一种计算地区具体预测产量及其发生机率的方法。一个基于利用初始土壤含水量、日降水、平均气温和太阳辐射为输入量的放牧地饲草模型,来计算实际蒸发量(T)

关键词：产量气象记录

Troubleshooting a Difficult Trans-Tibial/Fibula Amputation: A Case Report

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Surgical Science 2023年第6期14卷 414-427页

作者： Amy C. ross Alej.ndra Navarro Castaneda j.red D. Stucki Katharine A. Dishner Marisse A. Lardizabal Bryan j. Roth Luis G. Fernandez Samantha A. Delapena Marc R. Matthews Department of Podiatry Valleywise Health Medical Center Phoenix Arizona USA Department of Surgery Arrowhead Medical Center Glendale Arizona USA Department of Surgery Mountain Vista Medical Center Mesa Arizona USA Valleywise Health Medical Center Phoenix Arizona USA Department of Surgery Division of Trauma Surgery/Surgical Critical Care The University of Texas Health Science Center Tyler Texas USA School of Medicine Bill Barrett Endowed Chair in Trauma Surgery The University of Texas-Tyler Tyler Texas USA Trauma Wound Care UT Health East Tyler Texas USA Department of Surgery University of Texas Medical Branch Galveston Texas USA Department of Medicine and Nursing University of Texas Arlington Texas USA Past Commanding General TXSG Medical Brigade Austin Texas USA Department of Surgery Weill Cornell Medical Center New York NY USA Department of Surgery New York Presbyterian Hospital New York NY USA Department of Surgery Banner University of Arizona/University Medical Center Tucson Arizona USA

Necrotic feet secondary to vascular compromise in the diabetic patient may require an emergent guillotine amputation. Unrecognized, retained hardware in a distal ankle years after fracture repair may complicate the intraoperative guillotine amputation at the transtibial/fibula level. Troubleshooting such an unexpected surgical problem is not necessarily straightforward depending on the clinical situation. Presented is a case report where a patient with a necrotic burned foot failed to inform the burn team that he had implanted ankle hardware, prior to his surgical intervention. A successful amputation was completed after proceeding down a specific algorithm devised for such a scenario.

关键词： Guillotine Amputation Difficult Amputation Amputation Retained Hardware Titanium Plate Trans-Tibia Amputation

Genomic stability at the coding regions of the multidrug transporter gene ABCB1: insights into the development of alternative drug resistance mechanisms in human leukemia cells

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Cancer Drug Resistance 2020年第4期3卷 959-979页

作者： Kevin G.Chen George E.Duran Mark j.Mogul Yan C.Wang Kevin L.ross j.an-Pierre j.ffrézou Lyn M.Huff Kory R.j.hnson Tito Foj. Norman j.Lacayo Branimir I.Sikic Division of Oncology Department of MedicineStanford University School of MedicineStanfordCA 94305USA Current Address:NIH Stem Cell Unit National Institute of Neurological Disorders and StrokeNational Institutes of HealthBethesdaMD 20892USA Current Address:Medical Affairs U.S. Servier PharmaceuticalsBostonMA 02210USA Current Address:ross BioPharm Group Rocky PointNY 11778USA Current Address:French National Centre for Scientific Research Paris 75016France Medicine Branch National Cancer InstituteNational Institutes of HealthBethesdaMD 20892USA Current Address Laboratory of Cell BiologyNational Cancer InstituteNational Institutes of HealthBethesdaMD 20892USA Intramural IT and Bioinformatics Program National Institute of Neurological Disorders and StrokeNational Institutes of HealthBethesdaMD 20892USA Current Address:Herbert Irving Comprehensive Cancer Center Columbia University Medical Center/New York-Presbyterian HospitalNew YorkNY 10032USA Division of Pediatric Hematology-Oncology-Stem Cell Transplantation and Cancer Biology Stanford University School of Medicine and Stanford Cancer InstitutePalo AltoCA 94305USA

Aim:Despite considerable efforts to reverse clinical multidrug resistance(MDR),targeting the predominant multidrug transporter ABCB1/P-glycoprotein(P-gp)using small molecule inhibitors has been unsuccessful,possibly due to the emergence of alternative drug resistance ***,the non-specific P-gp inhibitor cyclosporine(CsA)showed significant clinical benefits in patients with acute myeloid leukemia(AML),which likely represents the only proof-of-principle clinical trial using several generations of MDR ***,the mutational mechanisms that may underlie unsuccessful MDR modulation by CsA are not elucidated because of the absence of CsA-relevant cellular *** this study,our aims were to establish CsA-resistant leukemia models and to examine the presence or absence of ABCB1 exonic mutations in these models as well as in diverse types of human cancer samples including ***:Drug-resistant lines were established by stepwise drug co-selection and characterized by drug sensitivity assay,rhodamine-123 accumulation,[3H]-labeled drug export,ABCB1 cDNA sequencing,and RNase protection *** genomic stability of the ABCB1 coding regions was evaluated by exome sequencing analysis of variant allele frequencies in human ***,the mutational spectrum of ABCB1 was further assessed in diverse types of cancer samples including AMLs in the Cancer Genome Atlas(TCGA)at the National Cancer ***:We report the development of two erythroleukemia variants,RVC and RDC,which were derived by stepwise co-selection of K562/R7 drug-resistant leukemia cells with the etoposide-CsA and doxorubicin-CsA drug combinations,***,both RVC and RDC cell lines,which retained P-gp expression,showed altered multidrug-resistant phenotypes that were resistant to CsA ***,no mutations were found in the ABCB1 coding regions in these variant cells even under long-term stringent drug ***,ABCB1

关键词： Cancer leukemia multidrug resistance ABCB1 P-glycoprotein cyclosporine mutation

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CH_4/CO_2重整反应中压力的影响

化工学报 1999年第6期50卷 856-859页

作者：赵金保李永丹田磊张鎏 j.lianR.H.ross Arj.nN.j.vanKeulen 天津大学化工学院催化科学与工程系碳一化工国家重点实验室天津300072 Limerick大学化学与环境科学系

近年来,CH_4与CO_2反应（式1）制合成气,已成为化工和催化领域的国际研究热点。由于体系中CO分压很高,极易积碳,因而催化剂的失活是所有研究者面临的主要问题。一些研究者研制的催化剂可在几十至几百小时内不失活,但相当多是在高CO_2/C... 详细信息

近年来,CH_4与CO_2反应（式1）制合成气,已成为化工和催化领域的国际研究热点。由于体系中CO分压很高,极易积碳,因而催化剂的失活是所有研究者面临的主要问题。一些研究者研制的催化剂可在几十至几百小时内不失活,但相当多是在高CO_2/CH_4比、高温或低压下获得的结果,这种条件的实际意义十分有限。实际生产时希望在接近反应（1）的计量进料比下操作,以获得满意的产物组成;希望在较低温度下操作,以降低能耗,或降低对反应器的要求;对于操作压力,由于原料均有一定压力,而且为了与合成气作为原料的后续生产过程相匹配,工业生产希望CH_4与CO_2反应能在一定压力下进行。

关键词：重整反应合成气铂催化剂催化剂失活

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An external quantum efficiency of>20%from solutionprocessed poly(dendrimer)organic light-emitting diodes

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npj.Flexible Electronics 2018年第1期2卷 213-218页

作者： Fatemeh Maasoumi ross D.j.nsen-van Vuuren Paul E.Shaw Emma V.Puttock Ravi Chandra Raj. Nagiri j.ke A.McEwan Mark Bown j.nny L.O’Connell Christopher j.Dunn Paul L.Burn Ebinazar B.Namdas Centre for Organic Photonics&Electronics(COPE) School of Chemistry&Molecular Biosciences and School of Mathematics&PhysicsUniversity of QueenslandSt.LuciaQLD 4072Australia CSIRO Manufacturing Research WayClaytonVIC 3168Australia

Controlling the orientation of the emissive dipole has led to a renaissance of organic light-emitting diode(OLED)research,with external quantum efficiencies(EQEs)of>30%being reported for phosphorescent *** highly efficient OLEDs are generally manufactured using evaporative methods and are comprised of small-molecule heteroleptic phosphorescent iridium(III)complexes blended with a host and additional layers to balance charge inj.ction and *** area OLEDs for lighting and display applications would benefit from low-cost solution processing,provided that high EQEs could be ***,we show that poly(dendrimer)s consisting of a non-conj.gated polymer backbone with iridium(III)complexes forming the cores of firstgeneration dendrimer side chains can be co-deposited with a host by solution processing to give highly efficient *** bilayer devices comprising the emissive layer and an electron transport layer gave an EQE of>20%at luminances of up to≈300 cd/m^(2),showing that polymer engineering can enable alignment of the emissive dipole of solution-processed phosphorescent materials.

关键词： quantum dipole light

Evolving mantle convection from bottom up to top down

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The Innovation 2022年第6期3卷 13-14页

作者： ross N.Mitchell Michael Brown Thomas M.Gernon Christopher j.Spencer State Key Laboratory of Lithospheric Evolution Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Laboratory for Crustal Petrology Department of GeologyUniversity of MarylandCollege ParkMD 20742USA School of Ocean and Earth Science University of SouthamptonSouthampton SO224JRUK Department of Geological Sciences and Geological Engineering Queen’s UniversityKingstonON K7L 2N8Canada

When it comes to convection,what goes up must come *** is it,what goes down must come up?The truth is,it *** convection must be mass-balanced,there is no reason that it must be force-balanced:the positive and negative buoyancy forces driving convection up and down,respectively,do not necessarily need to be *** balance,or imbalance,all depends on the top and bottom boundary layers.

关键词： necessarily boundary hough

Comparison of Behavioral Characteristics between Korean Native Chickens and Commercial Broilers

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j.urnal of Agricultural Science and Technology(A) 2011年第8期1卷 1329-1332页

作者： j. j. Ha B. C. Kim S. j. Ohh Y. H. Song College of Animal Life Science Kangwon National University Chuncheon 200-701 Gnagwon South Korea

Commercial broilers （CB） have been generally reared in Korea; however, Korean native chicken （KNC） is also bred for meat production. This study was performed to evaluate the behavioral characteristics of Korean native chickens （Han-hyup） and commercial broilers （ross 308）. In the study, KNC and CB were separately housed in 4 pens （30 birds/pen）, so there were two replicates for each treatment. Chickens were observed by video recording throughout the 8 weeks growth period for 12 hours each day. The time spent （standing, sitting, and walking） and frequencies （drinking, feeding, preening, dust-bathing, flapping, aggressive pecking, and ground pecking） of chicken＇s behavior were evaluated. Behavior was compared between the earlier period （1 to 4 weeks） and the later one （5 to 8 weeks） to know the changes in each behavior as they matured. Results showed that more time was spent on standing by KNC than CB. On the contrary, time spent for sitting behavior was less in KNC. The frequencies of most behaviors in KNC were higher than CB in the earlier period except feeding and dust-bathing. The greatest decreasing behavior as they matured was walking in both treatments, and a large number of commercial broilers did not walk, but instead rested as days passed. Feeding behavior of CB was more frequent in the earlier stage, but gradually reversed in the later period. The active behaviors such as fe, eding, preening, pecking, flapping and aggressive pecking in the KNC group were higher than the CB group, declining as time passed in both treatments; except dust-bathing that showed opposite pattern. These results were showed that KNC are much more active compared to CB, especially for walking, preening and pecking behavior. This may conclude that they may have fewer tendencies to develop potential health problems like dermatitis or even disease like sudden death syndrome.

关键词： Korean native chickens broiler behavior