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Evolving technologies for growing,imaging and analyzing 3d root system architecture of crop plants

2011年浙江省超声医学学术年会

作者： department of diagnostic Ultrasound & Echocardiography,Sir Run Run shaw Hospital,Zhejiang University College of Medicine & Sir Run Run shaw Institute of Clinical Medicine of Zhejiang University,Hangzhou, (310016) China XIONG li ZHAO Bowen LI Peng

Objective We hypothesized that in chronic fetal anemia,remodeling of the myocardium is related to abnormalities in regional wall motion and acutely increased afterload further disturbs myocardial strain. Methods Chronic anemia was induced in one fetus of each of seven sheep twin *** fetuses were studied by two-dimensional(2-d) strain echocardiography at baseline and during increased afterload via angiotensinⅡ(ATⅡ) infusion.

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Journal of Integrative Plant Biology 2016年第3期58卷 230-241页

作者： Miguel A.Pieros Brandon G.Larson Jon E.Shaff david J.Schneider Alexandre Xavier Falcao Lixing Yuan Randy T.Clark Eric J.Craft Tyler W.davis Pierre-Luc Pradier Nathanael M.shaw Ithipong Assaranurak Susan R.Mc Couch Craig Sturrock Malcolm Bennett Leon V.Kochian USdA-ARS Robert Holley Center for Agriculture and Health538 Tower RoadIthacaNY 14580USA department of Information Systems Institute of ComputingUniversity of CampinasAv.Albert Einstein1251CEP 13083-852CampinasSPBrazil department of Plant Nutrition China Agricultural UniversityBeijing 100193China department of Plant Breeding and Genetics Cornell UniversityIthacaNY 14853USA Centre for Plant Integrative Biology School of BiosciencesUniversity of NottinghamSutton BoningtonLE12 5RDUK College of Science King Saud UniversityRiyadh 11451Kingdom of Saudi Arabia

A plant＇s ability to maintain or improve its yield under limiting conditions,such as nutrient de ficiency or drought,can be strongly in fluenced by root system architecture（RSA）,the three-dimensional distribution of the different root types in the soil. The ability to image,track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying RSA. We have designed a hydroponic growth system which retains the three-dimensional RSA of the plant root system,while allowing for aeration,solution replenishment and the imposition of nutrient treatments,as well as high-quality imaging of the root system. The simplicity and flexibility of the system allows for modi fications tailored to the RSA of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity（detection of fine roots and other root details）,higher ef ficiency,and a broad array of growing conditions for plants that more closely mimic those found under field conditions.

关键词： Abiotic stress digital root phenotyping mineral nutrition root system architecture

Structural basis for the hydrolysis and transglycosylation activities of endo-β-N-acetylglycosaminidase A from Arthrobacter protophormiae

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Structural basis for the hydrolysis and transglycosylation a...

Comparison of Coopdech Endobronchial Blocker,Arndt Wire-guided Endobronchial Blocker,and Univent Tube with double-lumen Endotracheal Tube in video assisted thoracoscopic surgery A Randomized,Prospective,Controlled Trial

中国晶体学会第四届全国会员代表大会暨学术会议

作者： Jie Yin Lei Li Neil shaw Yang Li Chongyun Cheng Rongguang Zhang Peng Wang Zhi-Jie Liui National Glycoengineering Research Center and The State Key Laboratory of Microbial Technology School of Life ScienceShandong University National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of Sciences Structural Biology Center Advanced Photon SourceArgonne National LaboratoryIL 60439 USA

Endo-β-N-acetylglycosaminidases(ENGases) belonging to the GH85 families of glycoside hydrolases are dual specificity enzymes with an ability to catalyze hydrolysis and transglycosylation *** and transglycosylation are achieved via a unique substrate assisted ***,these enzymes have been the focus of intense research because of their potential for synthesis of novel,regio-and sterio-specific glycopeptides and *** of the 3d structure has severely hampered the rational engineering of these enzymes. We have determined the three dimensional structure of ENGase from Arthrobacter protophormiae(Endo-A) in complex with GlcNAc-thiazoline and ***-A is composed of three domains-a classic(β/α) TIM-barrel domain found in several GH18 family ENGases,a Carbohydrate Binding Module(CBM) and an Fn3 domain,which have never been reported before for *** carbohydrate moiety sits in a cleft region surrounded by aromatic residues just above the *** conserved essential catalytic residues-E173,N171 and Y205 are within hydrogen bonding distance of the substrate.W216 and W244 regulate access to the active site during transglycosylation by serving as "gate-keepers".Residues N241,F243 and Y299 play a role in transglycosylation due to their proximity to ***,Y299F mutation resulted in a 3 fold increase in the transglycosylation *** structure provides insights into the catalytic mechanism at molecular level and could assist rational engineering of ENGases.

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Comparison of Coopdech Endobronchial Blocker,Arndt Wire-guid...

2008年第七次华东六省一市麻醉学学术会议暨浙江省麻醉学术年会

作者： Taidi Zhong,M.d.,pHd,Wenyuan Wang,Md,Jie Chen,Md.Lv Ran,Md,pHd. department of Anesthesiology,Sir Run Run shaw Hospital,Medical College,Zhejiang University,Hangzhou City,China

Background:Techniques for one-lung ventilation(OLV)can be accomplished in two basic ways,the double lumen endobronchial tube(dLT)and the endobronchial blocker,such as the Amdt blocker or the Univent *** Coopdech endobronchial blocker is a newly designed endobronchial *** a few published articles described its use in *** current study was designed to compare the effectiveness of the Coopdech blocker with the Amdt blocker,the Univent blocker and the dLT during the elective video assisted thoracoscopic surgical(VATS)cases.

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恶性组织细胞病骨显像一例

中华核医学杂志 2003年第1期23卷 54-54,62页

作者：冯珏张琳边艳珠河北省人民医院核医学科石家庄050051

患者女,60岁,全身多发性骨痛2个月,伴发热,皮肤斑疹3d.肝脾及全身淋巴结未触及种大(入院后15d触诊脾肋下3cm),全身骨疼痛,活动不受限.实验室检查:WBC3.2×10~9/L,血红蛋白(HGB)84g/L,PLT78×10~9/L,红细胞沉降率:30mm/1h,60mm/2h,尿本... 详细信息

患者女,60岁,全身多发性骨痛2个月,伴发热,皮肤斑疹3d.肝脾及全身淋巴结未触及种大(入院后15d触诊脾肋下3cm),全身骨疼痛,活动不受限.实验室检查:WBC3.2×10~9/L,血红蛋白(HGB)84g/L,PLT78×10~9/L,红细胞沉降率:30mm/1h,60mm/2h,尿本周蛋白阴性.胸部GT示:双侧胸腔积液,左肺部分萎缩,右肺野内班片,索状影,考虑恶性组织细胞病肺仙浸泣表现,肿瘤不除外.2次骨髓涂片检查均考虑恶性组织细胞增生症.因临床不除外全身骨转移瘤而行全身骨显像,示全身骨髂显示不佳,软组织(肌肉及肺)显影,肝,脾放射性浓聚增高,于后位右肺下叶基底段可见一圆形放射性浓聚灶,与CT.X线胸片所示部位相符,性质待定.患者于骨显像后第15天死亡.

关键词：恶性组织细胞病骨显像诊断

中国四川盆地八角场气田低起伏滑脱褶皱作用的机理

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国外石油动态 2008年第7期 8-24页

作者： Andreas Plesch John H. shaw david Kronman 谢力（译）

我们使用三维（3d）地震反射数据和建立在力学约束基础上的新的二维（2d）和3d构造恢复方法来深入地了解中国四川盆地八角场背斜的发育。该构造形成了对八角场气田的圈闭，据报告，该气田在很厚的上三叠统三角洲前沿和湖岸相硅质碎屑岩... 详细信息

我们使用三维（3d）地震反射数据和建立在力学约束基础上的新的二维（2d）和3d构造恢复方法来深入地了解中国四川盆地八角场背斜的发育。该构造形成了对八角场气田的圈闭，据报告，该气田在很厚的上三叠统三角洲前沿和湖岸相硅质碎屑岩内含有大量天然气储量。基底滑脱位于中三叠统蒸发岩单元，在此之上的层根据它们的机械应力被折叠和断裂为各种密度。新的恢复方法使得我们能够探测各种断层位移方向，并断定八角场构造主要是通过倾滑和挤缩褶皱作用生长的。此外，我们量化了各构造层内缩短的样式和大小。基底滑脱层上的大量缩短和延性增厚与一般的滑脱褶皱模型一致。然而，我们证实，基底滑脱上的滑动要远大于这些模型预测的，这意味着基底滑脱上的滑动超出了构造范围进入了前陆。此外，褶皱核心中的构造增厚被产生了构造叠瓦的基底滑脱中的一系列小对冲断层局部化了。这意味着八角场滑脱褶皱是通过类似于冲褶席的机理生长的，这对在褶皱的不同机械层内划分缩短和应变的方式有重要意义。

关键词：滑脱褶皱褶皱作用四川盆地八角气田机理中国构造形成

Eating and Savoring Tea

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Women of China 1996年第8期 52-52页

作者： JANE shaw

TEA originated in China and spread to the rest of the world long ago. It is more than a popular beverage—it is a delicacy which can be both eaten and drunk, and around which many customs and rituals have been formed. Historical records show that tea leaves were first eaten, starting from the Spring and Autumn Period (770-476 B.C.). They were not steeped as a beverage until the early Western Han dynasty (206 B.C.-23 A.d.). More formal tea-drinking

关键词： Eating and Savoring Tea

Mutations in foregut SOX2^+ cells induce efficient proliferation via CXCR2 pathway

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Protein & Cell 2019年第7期10卷 485-495页

作者： Tomoaki Hishida Eric Vazquez-Ferrer Yuriko Hishida-Nozaki Ignacio Sancho-Martinez Yuta Takahashi Fumiyuki Hatanaka Jun Wu Alejandro Ocampo Pradeep Reddy Min-Zu Wu Laurie Gerken Reuben J. shaw Concepcion Rodriguez Esteban Christopher Benner Hiroshi Nakagawa Pedro Guillen Garcia Estrella Nunez delicado Antoni Castells Josep M. Campistol Guang-Hui Liu Juan Carlos Izpisua Belmonte Gene Expression Laboratory Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Advaneed Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric Disorders Xuanwu Hospital Capital Medical University Beijing 100053 China National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of the Chinese Academy of Sciences Beijing 100049 China Insitute for Stem Cell and Regeneration Chinese Academy of Sciences Beijing 100101 China Beijing Institute for Brain disorder Beijing 100069 China Universidad Catolica San Antonio de Murcia Campus de los Jeronimos 135 Guadalupe 30107 Spain Molecular and Cell Biology Laboratory Dulbecco Center for Cancer Research Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Howard Hughes Medical Institute Dulbecco Center for Cancer Research Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Integrative Genomics Core Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA division of Gastroenterology Department of Medicine Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA Abramson Cancer Center University of Pennsylvania Philadelphia PA 19104 USA department of Traumatology and Research Unit Clinica CEMTRO Av. Ventisquero de la Condesa 42 Madrid 28035 Spain Gastroenterology department Hospital Clinic University of Barcelona IDIBAPS CIBEREHD Barcelona 08036 Spain

Identification of the precise molecular pathways involved in oncogene-induced transformation may help us gain a better understanding of tumor initiation and promotion. Here, we demonstrate that SOX2^+ foregut epithelial cells are prone to oncogenic transformation upon mutagenic insults, such as Kras^G12d and p53 deletion. GFP-based lineage-tracing experiments indicate that SOX2^+ cells are the cells-of-origin of esophagus and stomach hyperplasia. Our observations indicate distinct roles for oncogenic KRAS mutation and P53 deletion. p53 homozygous deletion is required for the acquisition of an invasive potential, and Kras^G12d expression, but not p53 deletion, suffices for tumor formation. Global gene expression analysis reveals secreting factors upregulated in the hyperplasia induced by oncogenic KRAS and highlights a crucial role for the CXCR2 pathway in driving hyperplasia. Collectively, the array of genetic models presented here demonstrate that stratified epithelial cells are susceptible to oncogenic insults, which may lead to a better understanding of tumor initiation and aid in the design of new cancer therapeutics.

关键词： Sox2 tumor CXCR2 stratified epithelia

促进机器人辅助前列腺癌根治术术后早期尿控恢复的手术技巧(“大家泌尿网”观看手术视频)

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现代泌尿外科杂志 2021年第8期26卷 627-631页

作者：古迪王铭吴祥坤 SRIdHAR Ashwin N RAJAN Prabhakar SOORIAKUMARAN Prasanna shaw Greg NATHAN Senthil KELLY John d BRIGGS Tim P 广州医科大学附属第一医院泌尿外科广东广州510120 University College London Hospital London NW12BU

机器人辅助前列腺癌根治术(RARP)目前已经成为局限性有临床意义前列腺癌的根治性治疗的新的金标准。本文介绍了既往研究中通过RANd/UCLA改良法确认和研讨的16项促进RARP术后早期尿控恢复的手术技巧,并对各手术技巧进行了介绍。

关键词：机器人辅助前列腺癌根治术尿控手术技巧

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日粮成分对生长猪饮水量的影响

国外畜牧学（猪与禽） 2007年第6期27卷 25-28页

作者： M. I. shaw A. d. Beaulieu J. F. Patience 陈春月（译）覃矜（校）樊薛安（摘）加拿大Prairie猪中心研究公司加拿大萨斯卡彻温大学畜禽科学系不详

1 前言历史上,人们一直把水看成是充裕而廉价的资源,所以缺乏确定猪需水量或对影响水利用情况因素进行研究（Fraser等,1990）的积极性。现在,人类社会正越来越意识到水是有限的资源,而家畜生产行业却又是一个主要的耗水行业之一。对肉... 详细信息

1 前言历史上,人们一直把水看成是充裕而廉价的资源,所以缺乏确定猪需水量或对影响水利用情况因素进行研究（Fraser等,1990）的积极性。现在,人类社会正越来越意识到水是有限的资源,而家畜生产行业却又是一个主要的耗水行业之一。对肉猪生产者来说，过度用水会产生过多的厩粪，而厩粪储存和田间施用的费用都很高。因此，肉猪生产者日益有兴趣于既能减少用水量又不危害猪生长性能的管理方法。日粮调整不失为一个可能解决问题的策略。

关键词：饮水量生长猪日粮成分利用情况人类社会家畜生产生长性能日粮调整