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Real-time cell analysis and heat shock protein gene expression in the TcA Tribofium castaneum cell line in response to environmental stress conditions

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Insect Science 2017年第3期24卷 358-370页

作者： andres garcia-reina Maria Juliana Rodriguez-garcia Guillermo Ramis Jose Galian Department of Zoology and Physical Anthropology University of MurciaCampus Mare Nostrum E-30100 Murcia Spain Department of Animal Production Faculty of Veterinary University of MurciaCampus Mare Nostrum E-30100 Murcia Spain

The rust red flour beetle, Tribolium castaneum （Herbst, 1797）（Coleoptera： Tenebrionidae）, is a pest of stored grain and one of the most studied insect model species. Some of the previous studies involved heat response studies in terms of survival and heat shock protein expression, which are regulated to protect other proteins against environ- mental stress conditions. In the present study, we characterize the impedance profile with the xCELLigence Real-Time Cell Analyzer and study the effect of increased temperature in cell growth and viability in the cell line BCIRL-TcA-CLG 1 （TcA） of T castaneum. This novel system measures cells behavior in real time and is applied for the first time to insect cells. Additionally, cells are exposed to heat shock, increased salinity, acidic pH and UV-A light with the aim of measuring the expression levels of lisp27, Hsp68a, and Hsp83 genes. Results show a high thermotolerance of TeA in terms of cell growth and viability. This result is likely related to gene expression results in which a significant up-regulation of all studied Hsp genes is observed after 1 h of exposure to 40 ~C and UV light. All 3 genes show similar expression patterns, but Hsp27 seems to be the most affected. The results of this study validate the RTCA method and reveal the utility of insect cell lines, real-time analysis and gene expression studies to better understand the physiological response of insect cells, with potential applications in different fields of biology such as conservation biology and pest management.

关键词： environmental stress gene expression heat shock proteins insect cells,real-time cell analysis (RTCA) red flour beetle

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Challenges and improvement strategies in the hospitalization of chronic multimorbid patients

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World Journal of Clinical Cases 2025年第3期13卷 35-41页

作者： andres Fontalba-Navas Francisco Pozo Muñoz Rogelio garcia Cisneros Maria Jose garcia Larrosa Maria del Mar Callejon Gil Ignacio garcia Delgado Maria Belen Jimenez Martinez Antequera Hospital Northern Málaga Integrated Healthcare AreaAntequera 29200AndalusiaSpain Department of Public Health and Psychiatry University of MálagaMálaga 29010Spain Antequera Hospital Northern Málaga Antequera Integrated Healthcare AreaAntequera 29200MálagaSpain

BACKGROUND Addressing the growing challenge of hospitalizing chronic multimorbid patients,this study examines the strain these conditions impose on healthcare systems at a local level,focusing on a pilot *** diseases and complex patients require comprehensive management strategies to reduce healthcare burdens and improve patient *** proven effective,this pilot model has the potential to be replicated in other healthcare settings to enhance the management of chronic multimorbid *** To evaluate the effectiveness of the high complexity unit(HCU)in managing chronic multimorbid patients through a multidisciplinary care model and to compare it with standard hospital *** The study employed a descriptive longitudinal approach,analyzing data from the Basic Minimum Data Set(BMDS)to compare hospitalization variables among the HCU,the Internal Medicine Service,and other services at Antequera Hospital throughout *** HCU,designed for patients with complex chronic conditions,integrates a patient-centered model emphasizing multidisciplinary care and continuity *** The study employed a descriptive longitudinal approach,analyzing data from the BMDS to compare hospitalization variables among the HCU,the Internal Medicine Service,and other services at Antequera Hospital throughout *** HCU,designed for patients with complex chronic conditions,integrates a patient-centered model emphasizing multidisciplinary care and continuity *** This study demonstrates the effectiveness of the HCU in managing patients with complex chronic diseases through a multidisciplinary *** coordinated care provided by the HCU results in improved patient outcomes,reduced unnecessary hospitalizations,and better management of patient *** superiority of the HCU compared to standard care is evident in key outcomes such as fewer readmissions and higher patient satisfaction,reinforcing its value as a model of ca

关键词： High complexity unit Chronic multimorbidity Patient-centered care Integrated care Healthcare management

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Different maternal investment strategies for male and female calves in a polygynous mammal

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Current Zoology 2019年第3期65卷 269-277页

作者： Francisco CEACERO Martina KOMARKOVA andres J.garcia Laureano GALLEGO Department of Animal Science and Food Processing Faculty of Tropical AgriSciencesCzech University of Life Sciences Kamycka 12916500 Praha 6-Suchdol Czech Republic Department of Ethology Institute of Animal Science Pratelstvi 815 10401 Praha - Uhnneves Czech Republic Instituto de Investigacion en Recursos Cinegeticos (IREC) Consejo Superior de Investigaciones Cientificas-Universidad de Castilla-La Mancha-Junta de Comunidades de Castilla-La Mancha (CSIC-UCLM-JCCM) Ronda de Toledo s/n 13071 Ciudad RealSpain Seccion de Recursos Cinegeticos y Ganaderos Instituto de Desarrollo Regional (IDR)02071 AlbaceteSpain Departamento de Ciencia y Tecnologia Agroforestal y Genetica ETSIAM Universidad de Castilla-La Mancha(UCLM)02071 AlbaceteSpain

Mater nal effects occur whe n the phe no type of the mother in flue nces that of the young to the detriment of her survival, growth or fitn ess. The in vestment of the mother can be affected by mater nal body condition and/or experience. Trivers-Willard Hypothesis (TWH) and Local Resource Competition Hypothesis (LRCH) are the main hypotheses used to explain bias in birth sex-ratios in mammals, as well as for sex-biased maternal investment. Both hypotheses suggest that a different amount of investment must be expected according to the sex of the young. However, recent studies suggest that these differences are not in quantity but in the strategies: mechanisms and objectives may differ for each sex. We studied how maternal characteristics (age, body mass, body condition, and domi nance status) influence re leva nt aspects of the birth and early growth of the calf (birth date, birth body mass, body mass at weaning, and body condition at weaning) separately for each sex;and how that investment is mediated by milk production and composition (lactose, fat, and protein). One hundred eighty-eight newborns from 75 captive red deer hinds aged from 2 to 19 years were analyzed. The main differential investment observed was related to birth date: when producing a female, hinds give birth earlier in the season only if they have a good body condition;however, when gestating a male it is the older hinds those which deliver earlier. Subsequently, milk production and composition are correlated with birth body mass in female calves, but to weaning body mass in males. Thus, only hind body mass affects the weaning body mass of female calves, compared with age and hind body mass in males. These results suggest that while TWH fits the maternal investment strategy found for male calves, it is LRCH which correlates with the maternal investment patterns observed for females.

关键词： birth date body condition local resources competition hypothesis milk production sex-ratio Trivers-Willard hypothesis

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Fast preparation route to high-performances textured Sr-doped Ca_3Co_4O_9 thermoelectric materials through precursor powder modification

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Science China Materials 2019年第3期62卷 399-406页

作者： Miguel Angel Torres Gustavo garcia Idoia Urrutibeascoa Maria Antonieta Madre Juan Carlos Diez andres Sotelo ICMA (CSIC-Universidad de Zaragoza) Centro Stirling S. Coop. Mondragon Unibertsitatea

This work presents a short and very efficient method to produce high performance textured Ca_3Co_4O_9 thermoelectric materials through initial powders modification. Microstructure has shown good grain orientation, and low porosity while slightly lower grain sizes were obtained in samples prepared from attrition milled powders. All samples show the high density of around 96% of the theoretical *** similar characteristics are reflected in, approximately,the same electrical resistivity and Seebeck coefficient values for both types of samples. However, in spite of similar power factor(PF) at low temperatures, it is slightly higher at high temperature for the attrition milled samples. On the other hand, the processing time reduction(from 38 to 2 h) when using attrition milled precursors, leads to lower mechanical properties in these samples. All these data clearly point out to the similar characteristics of both kinds of samples, with a drastic processing time decrease when using attrition milled precursors, which is of the main economic importance when considering their industrial production.

关键词： ceramics oxides hot-pressing electrical properties power factor

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Influence of V(C,N) Precipitates on Microstructure and Mechanical Properties of Continuous Cooled C-Mn-V

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Journal of Iron and Steel Research International 2011年第S1期18卷 266-270页

作者： Carlos garcia-Mateo Carlos Capdevila Juan Cornide Jesus Chao Francisca G Caballero Carlos garcia de andres Materalia Research Group Department of Physical Metallurgy Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC)Avda.Gregorio del Amo8 E-28040 MadridSpain

The effect of chemical composition and processing parameters on the formation of acicular ferrite and/or bainite has been *** particular,this paper deals with the influence that N through its combination with V,as V(C,N) precipitates,has on the decomposition of ***,the intragranular nucleation potency of V(C,N) precipitates is analyzed through the continuous cooling transformation diagrams (CCT) of two C-Mn-V steels with different contents of *** reported in this work allow us to conclude that acicular ferrite can only be achieved alloying with vanadium and nitrogen,meanwhile bainite is promoted in steels with a low level of *** is concluded that higher strength values are obtained in acicular ferrite than in bainitic steel but a similar brittle-ductile transition temperature (BDT),and lower values of impact absorbed energy (KV) has been recorded in nitrogen-rich steel.

关键词： acicular ferrite bainite vanadium precipitates mechanical properties

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Postural control quantification in minimally and moderately impaired persons with multiple sclerosis:The reliability of a posturographic test and its relationships with functional ability

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Journal of Sport and Health Science 2020年第6期9卷 677-684页

作者： David Barbado Ramon Gomez-Illan Pedro Moreno-Navarro Gregori Valero-Conesa Raul reina Francisco J.Vera-garcia Department of Sport Sciences Sports Research CentreMiguel Hern_andez University of ElcheElche(Alicante)03202Spain

Background:Postural control has been associated with the functional impairment in persons with multiple sclerosis(pwMS).However,there is a need for reliable methods to assess postural control in early stages of the disease,when subtle changes can be difficult to *** aims of this study were to assess the absolute and relative reliability of a standing and a sitting posturographic protocol in minimally(Expanded Disability Status Scale≤2)and moderately(2.5≤Expanded Disability Status Scale≤4)impaired pwMS,and to analyze relationships among postural control and functional mobility and gait ***:To assess postural control in an upright stance,14 minimally and 16 moderately impaired pwMS performed six 70 s trials in tandem stance,3 with their weaker leg behind(TSWL)and 3 with their stronger leg behind(TSSL).Additionally,participants completed five 70 s trials using an unstable sitting protocol(US)to assess trunk *** mean radial errors of TSWL,TSSL,and US trials were calculated as postural control ***,participants performed the Timed Up and Go test(TUG)and the Timed 25-foot Walk test(T25FW)to measure their functional mobility and gait speed,*** was evaluated using the intraclass correlation coefficient(ICC3,1)and the standard error of measurement(SEM).Analyses of variances were carried out to assess between-group ***’g index(dg)was used to estimate the effect size of *** correlation analyses(r)were performed to examine the relationships among the postural control and the functional ***:Posturographic tests showed a high reliability in both minimally(0.87≤ICC≤0.92;9.32%≤SEM≤11.76%)and moderately(0.80≤ICC≤0.92;10.33%≤SEM≤15.33%)impaired ***,T25FW and TUG displayed a high consistency in minimally(0.89≤ICC≤0.94;3.43%≤SEM≤5.17%)and moderately(0.85≤ICC≤0.93;5.57%≤SEM≤6.56%)impaired *** impaired pwMS showed a better performance on the TUG,T25FW

关键词： Balance Consistency of the assessment Core stability Functional mobility

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Interactions between maternal parity and feed additives drive the composition of pig gut microbiomes in the post‑weaning period

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Journal of Animal Science and Biotechnology 2024年第3期15卷 1241-1257页

作者： Kayla Law Eduardo Rosa Medina garcia Chad Hastad Deborah Murray Pedro E.Urriola andres Gomez Department of Animal Science University of Minnesota1364 Eckles AvenueSaint PaulMN 55108USA New Fashion Pork 164 Industrial ParkwayJacksonMN 56143USA

Background Nursery pigs undergo stressors in the post-weaning period that result in production and welfare *** challenges disproportionately impact the offspring of primiparous sows compared to those of mul-tiparous *** is known regarding potential interactions between parity and feed additives in the post-weaning period and their effects on nursery pig ***,the objective of this study was to investigate the effects of maternal parity on sow and offspring microbiomes and the influence of sow parity on pig fecal microbi-ome and performance in response to a prebiotic *** weaning,piglets were allotted into three treat-ment groups:a standard nursery diet including pharmacological doses of Zn and Cu(Con),a group fed a commercial prebiotic only(Preb)based on an Aspergillus oryzae fermentation extract,and a group fed the same prebiotic plus Zn and Cu(Preb+ZnCu).Results Although there were no differences in vaginal microbiome composition between primiparous and mul-tiparous sows,fecal microbiome composition was different(R^(2)=0.02,P=0.03).The fecal microbiomes of primiparous offspring displayed significantly higher bacterial diversity compared to multiparous offspring at d 0 and d 21 post-weaning(P<0.01),with differences in community composition observed at d 21(R^(2)=0.03,P=0.04).When analyzing the effects of maternal parity within each treatment,only the Preb diet triggered significant microbiome distinc-tions between primiparous and multiparous offspring(d 21:R^(2)=0.13,P=0.01;d 42:R^(2)=0.19,P=0.001).Composi-tional differences in pig fecal microbiomes between treatments were observed only at d 21(R^(2)=0.12,P=0.001).Pigs in the Con group gained significantly more weight throughout the nursery period when compared to those in the Preb+ZnCu *** Nursery pig gut microbiome composition was influenced by supplementation with an Aspergillus oryzae fermentation extract,with varying effects on performance

关键词： Aspergillus prebiotic Copper Feed additives Maternal Nursery pig microbiome Parity Swine microbiome Trace minerals Zinc

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Dysregulated immunity in PID patients with low GARP expression on Tregs due to mutations in LRRC32

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Cellular & Molecular Immunology 2021年第7期18卷 1677-1691页

作者： Peter Lehmkuhl Magdalena Gentz andres Caballero garcia de Otezya Bodo Grimbacher Hendrik Schulze-Koops Alla Skapenko Division of Rheumatology and Clinical Immunology Department of Internal Medicine IVLudwig-Maximilians-University of MunichMunichGermany Institute for Immunodeficiency Centre of Chronic ImmunodeficiencyMedical CentreFaculty of MedicineAlbert-Ludwigs-University of FreiburgFreiburgGermany

Immune dysregulation diseases are characterized by heterogeneous clinical manifestations and may have severe disease *** identification of the genetic causes of these diseases therefore has critical clinical *** performed whole-exome sequencing of patients with immune dysregulation disorders and identified two patients with previously undescribed mutations in LRRC32,which encodes glycoprotein A repetitions predominant(GARP).These patients were characterized by markedly reduced numbers and frequencies of regulatory T cells(Tregs).Tregs with mutated LRRC32 exhibited strongly diminished cell-surface GARP expression and reduced suppressor *** a model of conditional Garp deficiency in mice,we confirmed increased susceptibility to inflammatory diseases once GARP expression on Tregs was *** deficiency led to an unstable Treg phenotype due to diminished Foxp3 protein acetylation and *** study reinforces the understanding of the immunological mechanisms of immune dysregulation and expands the knowledge on the immunological function of GARP as an important regulator of Treg stability.

关键词： T cells immune dysregulation autoimmunity

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QUALITATIVE ANALYSIS OF SUSTAINABLE VERTICAL DWELLING IN NUEVO LEON, MEXICO

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Journal of Green Building 2011年第1期6卷 58-72页

作者： Pedro Morales Juan P.Solis andres Trillo Salvador garcia Civil Engineering Department.ITESM Monterrey Campus ITESM Monterrey Campus GGP

Climate change has become a big concern nowadays since the effects of fluctuation in the weather are more notable every *** Mexico construction industry generates around 33%of the countries CO_(2) emissions,and 7.64%amount for housing *** Vertical Dwelling was considered as a suitable solution to cover the housing shortage and lower the impact to the *** assessment of awareness of sustainability was carried out in the housing sector including government,developers and *** interviews were designed one for each kind of *** with the outcome of the interviews,guidelines to address sustainability in the housing sector were *** ongoing project was selected and a qualitative comparison analysis was established,making green improvements and discussing the impact of such *** an outcome we observed that sustainability is getting attention from the government and *** Housing Found has launched the Green Mortgage to promote green housing for the low income families,this program could be expanded to higher income classes and for vertical *** is not a proper regulatory framework for the condominium regimen neither for the construction of green dwellings,thus,problems arise for the condominium *** developers are implementing some green features on their projects,but not as a mean to address sustainability,but as a marketing ***,vertical dwelling represents a greener way to build by the fact that it requires less land space,vacant lands within urban areas can be recovered,thus the use of existing infrastructure and services make the projects efficient and less expensive.

关键词： sustainability vertical dwelling condominium

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Analysis and Validation for an Inverter-side Current Controller in LCL Grid-connected Power Systems

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Journal of Modern Power Systems and Clean Energy 2020年第2期8卷 387-398页

作者： Jose MSosa Panfilo R.Martinez-Rodriguez Gerardo Escobar Gerardo Vazquez andres A.Valdez-Fernandez Juan F.Martinez-garcia the Tecnologico Nacional de Mexico/ITS de Irapuato(TecNM/ITESI) IrapuatoGTO 36821Mexico the School of Sciences Autonomous University of San Luis Potosi(UASLP)San Luis PotosiSLP 78290Mexico Tecnologico de Monterrey N.L.64849Mexico

Transformerless grid-connected inverters offer greater efficiencies when transferring power from renewable energy sources to the electrical *** the grid-inverter connection is done with an LCL filter,high attenuation of switching harmonics is achieved while preserving a small-size output ***,damping must be included in the controller to assure closed-loop *** paper proposes a reference computation methodology for the inverter-side current feedback in a photovoltaic(PV)generation system connected to the grid through an LCL *** analysis of the closed-loop system stability and of the steady-state performance are presented as well as experimental validation of the closed-loop *** feedback controller includes active damping and relies on a resonant control structure which improves the ability of dealing with grid harmonic *** controller uses a reduced set of measurements,which requires the inverter-side current and grid voltage only,and assures a power factor close to unity.

关键词： LCL filter transformerless inverter grid connection inverter-side current feedback

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