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Molecular and cellular changes in the post-traumatic spinal cord remodeling after autoinfusion of a genetically-enriched leucoconcentrate in a mini-pig model

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Neural Regeneration Research 2023年第7期18卷 1505-1511页

作者： Maria Aleksandrovna Davleeva Ravil Rasimovich Garifulin Farid Vagizovich Bashirov andrei aleksandrovich Izmailov Leniz Faritovich Nurullin Ilnur Ildusovich Salafutdinov Dilara Zilbarovna Gatina Dmitrij Nikolaevich Shcherbinin andrei aleksandrovich lysenko Irina Leonidovna Tutykhina Maksim Mikhailovich Shmarov Rustem Robertovich Islamov Department of Histology Cytology and EmbryologyKazan State Medical UniversityKazanRussia Кazan Institute of Biochemistry and Biophysics Federal Research Center of Kazan Scientific Center of Russian Academy of SciencesKazanRussia Institute of Fundamental Medicine and Biology Kazan Federal UniversityKazanRussia The National Research Center for Epidemiology and Microbiology named after Honorary Academician N.F.Gamaleya of the Ministry of Health of the Russian Federation MoscowRussia

Post-traumatic spinal cord remodeling includes both degenerating and regenerating processes,which affect the potency of the functional recovery after spinal cord injury(SCI).Gene therapy for spinal cord injury is proposed as a promising therapeutic strategy to induce positive changes in remodeling of the affected neural *** our previous studies for delivering the therapeutic genes at the site of spinal cord injury,we developed a new approach using an autologous leucoconcentrate transduced ex vivo with chimeric adenoviruses(Ad5/35)carrying recombinant *** the present study,the efficacy of the intravenous infusion of an autologous genetically-enriched leucoconcentrate simultaneously producing recombinant vascular endothelial growth factor(VEGF),glial cell line-derived neurotrophic factor(GDNF),and neural cell adhesion molecule(NCAM)was evaluated with regard to the molecular and cellular changes in remodeling of the spinal cord tissue at the site of damage in a model of mini-pigs with moderate spinal cord *** animals were randomly divided into two groups of 4 pigs each:the therapeutic(infused with the leucoconcentrate simultaneously transduced with a combination of the three chimeric adenoviral vectors Ad5/35‐VEGF165,Ad5/35‐GDNF,and Ad5/35‐NCAM1)and control groups(infused with intact leucoconcentrate).The morphometric and immunofluorescence analysis of the spinal cord regeneration in the rostral and caudal segments according to the epicenter of the injury in the treated animals compared to the control mini-pigs showed:(1)higher sparing of the grey matter and increased survivability of the spinal cord cells(lower number of Caspase-3-positive cells and decreased expression of Hsp27);(2)recovery of synaptophysin expression;(3)prevention of astrogliosis(lower area of glial fibrillary acidic protein-positive astrocytes and ionized calcium binding adaptor molecule 1-positive microglial cells);(4)higher growth rates of regeneratingβIII-tubulin-positi

关键词： autologous genetically-enriched leucoconcentrate chimeric adenoviral vector gene therapy glial cell line-derived neurotrophic factor mini-pig neural cell adhesion molecule spinal cord contusion injury vascular endothelial growth factor

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The Influence of Generated Pulses Parameters on the Thyratron in the Inductive Energy Storage

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Journal of Energy and Power Engineering 2012年第3期6卷 387-390页

作者： Vereschagin Nikolai Mihailovich Kruglov Sergey aleksandrovich Serezhin andrei aleksandrovich Ryazan State Radio Engineering University Ryazan 390005 Russia

Experimental research of anode current and anode voltage influence on the speed of decaying plasma resistance change and the time ofthyratron cutoff. Anode current resistance increase is shown to cause thyratron cutoff time increase in case of fixed anode voltage. In turn anode voltage increase in case of fixed anode current causes earlier increase of decaying plasma resistance growth speed and quicker apparatus cutoff：

关键词： Voltage inductive energy storages commutator thyratron plasma.

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