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Surveying the Oligomeric State of Arabidopsis thaliana Chloroplasts

Molecular Plant 2017年第1期10卷 197-211页

作者： Peter k. Lundquist Otho Mantegazza Anja Stefansk. k.i Stuhler andreas P.M. Weber institute for Plant Biochemistry Molecular Proteomics La_boratory BMFZ Cluster of Excellence on Plant Sciences (CEPLAS) Heinrich-Heine-University UniversitatsstraBe 1 40225 Duseldorf Germany present address: Plant Biology Division The Samuel Roberts Noble Foundation Ardmore OK 73401 USA

Blue native-PAGE （BN-PAGE） resolves protein complexes in their native state. When combined with immu- noblotting, it can be used to identify the presence of high molecular weight complexes at high resolution for any protein, given a suitable antibody. To identify proteins in high molecular weight complexes on a large scale and to bypass the requirement for specific antibodies, we applied a tandem mass spectrometry （MS/MS） approach to BN-PAGE-resolved chloroplasts. Fractionation of the gel into six bands allowed iden- tification and label-free quantification of 1000 chloroplast proteins with native molecular weight separation. Significantly, this approach achieves a depth of identification comparable with traditional shotgun proteo- mic analyses of chloroplasts, indicating much of the k.own chloroplast proteome is amenable to MS/MS identification under our fractionation scheme. By limiting the number of fractionation bands to six, we facil- itate scaled-up comparative analyses, as we demonstrate with the reticulata chloroplast mutant displaying a reticulated leaf phenotype. Our comparative proteomics approach identified a candidate interacting protein of RETICULATA as well as effects on lipid remodeling proteins, amino acid metabolic enzymes, and plastid division machinery. We additionally highlight selected proteins from each sub-compartment of the chloroplast that provide novel insight on k.own or hypothesized protein complexes to further illus- trate the utility of this approach. Our results demonstrate the high sensitivity and reproducibility of this technique, which is anticipated to be widely adaptable to other sub-cellular compartments.

关键词： chloroplast blue native-PAGE comparative proteomics protein complex oligomeric state,RETICULATA

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Putting On The Break.:Regulating Organelle Movements in Plant Cells

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Journal of Integrative Plant Biology 2012年第11期54卷 868-874页

作者： Julianna k.Vick.andreas Nebenfhr Department of Biochemistry and Cellular and Molecular Biology University of TennesseeKnoxvilleTN 37996USA

A strik.ng characteristic of plant cells is that their organelles can move rapidly through the cell. This movement, commonly referred to as cytoplasmic streaming, has been observed for over 200 years, but we are only now beginning to decipher the mechanisms responsible for it. The identification of the myosin motor proteins responsible for these movements allows us to probe the regulatory events that coordinate organelle displacement with normal cell physiology. This review will highlight several recent developments that have provided new insight into the regulation of organelle movement, both at the cellular level and at the molecular level.

关键词： Cytoplasmic streaming cytosk.leton myosin organelle.

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Osteolectin+stromal cells:Mechanical stimulation improves bone regeneration and supports bacterial clearance after fracture

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Signal Transduction and Targeted Therapy 2021年第8期6卷 2257-2258页

作者： Sabrina Ehnert Tina Histing andreas k.Nussler Department of Trauma and Reconstructive Surgery Eberhard k.rls University Tubingen Siegfried Weller Research InstituteBG Trauma Center TubingenTubingenGermany

In a study recently published in Nature,Shen and colleagues characterized a peri-arteriolar niche in the bone marrow with highly proliferative,short-lived,leptin receptor-and osteolectin(Oln)positive stromal cells,which improved bone regeneration and supported bacterial clearance after *** proving that maintenance of these cells critically required mechanical stimulation,which in turn was affected by age,1 the authors identified possible therapeutic targets for bone healing and regeneration.

关键词： stimulation healing clearance

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Highly Fuel-Efficient Transmission and Propulsion Concepts

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Automotive Innovation 2018年第1期1卷 35-42页

作者： Ferit k.çük.y andreas Lange Lin Li Institute of Automotive Engineering(IAE) Technische Universität Braunschweig38106 BraunschweigGermany

The integration of one or more electricmachines into the drivetrain has resulted inmany different powertrain concepts in recent years,ranging from the P2 hybrid to dedicated hybrid transmission(DHT).Two types of DHT with different characteristics are *** first type is the power split hybrid(PS-DHT),which has very low mechanical complexity but needs high electrical effort in the *** second type is multi-mode DHT(MM-DHT),which has a slightly higher mechanical complexity but much less electrical effort when compared with the PS-DHT.A transmission synthesis is used to determine the concept of the *** three different powertrain concepts(i.e.,P2,PS-DHT,and MM-DHT)are analyzed and evaluated regarding fuel economy and *** driving cycles(e.g.,Worldwide Harmonized Light vehicles Test Procedure)and the 3D method(driver,driven vehicle,driving environs)are used to investigate the drivetrain in the context of real driving *** show that the two DHT concepts offer better fuel economy than the P2 hybrid drivetrain while still providing the same or even better driving *** study also shows that new hybrid concepts created with transmission synthesis can lead to further improvements in hybrid powertrains.

关键词： Dedicated hybridtransmission Three-dimensional method Drivetraindesign Fuel economy Drivingperformance

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Regional ozone pollution and k.y controlling factors of photochemical ozone production in Pearl River Delta during summer time

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Science China Chemistry 2010年第3期53卷 650-662页

作者： CHANG ChihChung CHOU C.k.Charles andreas Wahner Research Center for Environmental Changes Academia SinicaTaipei 11529China Forschungszentrum Juelich ICG-Ⅱ:TroposphereJuelichGermany

An intensive field campaign including measurements from the environmental monitoring network.and from two super sites took.place in the Pearl River Delta region in summer *** routinely measured O3 and NOx concentrations,the spatial and temporal variation of O3 and of the total oxidant concentrations was *** to the spatial variability of NO2/NO,the two super sites were found to be representative of polluted urban and downwind suburban *** addition,both sites were located in high O3 ***-depth diagnostic of photochemical ozone production processes and their k.y controlling factors are achieved with an observation-based model(OBM) to gain regional *** analysis and sensitivity model runs show that aldehyde and HONO chemistry had significant impacts on local photochemical ozone production *** analysis of calculated Relative Incremental Reactivities shows that photochemical ozone production rates are mainly sensitive to anthropogenic hydrocarbons(HCs) in the polluted urban *** the suburban areas,sensitivity to nitrogen oxide(NO) concentrations *** anthropogenic HCs in both areas are alk.nes and *** differences of ozone production efficiencies are identified between the urban and suburban regions,consistent with the OBM diagnosed results.

关键词： ozone Pearl River Delta photochemical ozone productions HONO relative incremental reactivity

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Program & faculty evaluation

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Annals of Eye Science 2017年第1期2卷 220-228页

作者： andreas k.Lauer Casey Eye Institute Oregon Health and Science UniversityPortland OregonUSA

Graduate medical education(GME)has shifted its curricula from process-oriented approach to outcomes-oriented *** and faculty evaluation are methods by which educational curricula may adjust the teaching and learning environment to meet the needs and fills the gaps in *** measurement of educational outcomes is an essential for assessing teaching effectiveness in a shifting health care *** addition to trainee,program,and faculty evaluations,annual program review(APR)and evaluation and navigational changes made by the program education committee are essential to maintain effectiveness of an educational curriculum in a contemporary graduate medical training program.

关键词： Program evaluation faculty evaluation program evaluation committee(PEC) annual program review(APR) graduate medical education(GME)

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The Nordic geological surveys： Geology for society in practice

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Episodes 2008年第1期31卷 193-200页

作者： Morten Smelror andreas AhlstrФm Lena Ek.lund Jens Morten Hansen k.ijo Nenonen Anette k. Mortensen Geological Survey of Norway （NGU） Leiv Eirikssons vei 39 NO-7491 Trondheim Norway. Geological Survey of Denmark.and Greenland （GEUS） Фster Voldgade 10 DK-1350 KCbenhavn K Denmark. Geological Survey of Sweden （SGU） Box 670 S-751 28 Uppsala Sweden. Geological Survey of Finland （GTk. P.O. Box 96 FI-02151 Espoo Finland. Iceland Geosurvey （ISOR） Grensaisvegi 9 108 Reykjavfk.Ucekabd.

Since the mid-nineteenth century., when the first of the Nordic Geological Surveys were established, they have generated a substantial amount of information on the Earth＇s crust, its natural resources, its processes, and on the geological history of Nordic areas. The collective mission of the geological surveys is to carry out ＂Geology for Society＂, by doing research and providing services, and by mak.ng geological information and data easily accessible to all the varied end users in industry, government agencies, government institutes, public administrations, technical offices, academia and research institutes, as well as for private individuals. The present paper gives a brief overview of a few, selected, research areas and projects currently undertak.n by the Nordic geological surveys. These serve as practical examples of how the Nordic geological surveys address important societal problems and challenges that require geological input for their solution.

关键词：地质学测量北欧地区社会实践公共管理

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Melt emulsification-Is there a chance to produce particles without additives?

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Particuology 2011年第5期9卷 506-509页

作者： k.rsten k.hler andreas Hensel Manfred k.aut Heik. P. Schuchmann Food Process Engineering Karlsruhe Institute of Technology (KIT) Mik.overfahrenstechnik Karlsruhe Institute of Technology (KIT)

Melt emulsification is a well k.own process. Milk.is thus homogenized for over 100 years. In the melt emulsification process, the future disperse phase is melted and dispersed into droplets, the size of which is controlled by an emulsification process. After emulsification, the droplets are cooled down and solid particles of spherical shape are formed. In order to realize melt emulsification processes, we developed the new SHM （Simultaneous Homogenizing and Mixing） nozzle, which enables us to mix separate phases directly into the droplet forming zone of homogenization nozzles. This molten milk.fat globule can be homogenized at elevated fat content （up to 42 vol% instead of max. 17 vol%） and elevated temperatures （up to 150 ℃ instead of max. 70 ℃） without loosing product quality as for conventional homogenization processes. In addition, more than 80% of the energy costs can be saved and additional mixing units can be spared. This is realized by a controlled and quick.dilution and cooling down of molten fat globules directly after their disruption in the nozzle itself. SHM-technology also allows for the dispersing of molten waxes. Instant cooling down after adjusting particle sizes also allows us to work.without emulsifiers or other additives as absolutely required in conventional melt emulsification processes where molten droplets will coalesce upon their collisions in the homogenization nozzle. SHM-melt emulsification is thus an alternative to conventional milling processes, which are often limited by the stick.ness of these products.

关键词： Melt emulsification Dispersion Emulsion Homogenization Mixing Milk.CreamWax

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Optical screw-wrench for microassembly

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Microsystems & Nanoengineering 2017年第1期3卷 389-395页

作者： Jannis k.hler Sarah Isabelle k.ouri Cemal Esen andreas Ostendorf Applied Laser Technologies Ruhr-Universität BochumUniversitätsstraße 150Bochum 44801Germany

For future micro-and nanotechnologies,the manufacturing of miniaturized,functionalized,and integrated devices is *** this paper,an assembly technique based on a bottom-up strategy that enables the manufacturing of complex microsystems using only optical methods is presented.A screw connection is transferred to the micrometer range and used to assemble screwand nut-shaped ***-stereolithography is performed by means of two-photon polymerization,and microstructures are fabricated and subsequently trapped,moved,and screwed together using optical forces in a holographic optical tweezer *** design and construction of interlock.ng microcomponents and the verification of a stable and releasable joint form the main focus of this *** assembly technique is also applied to a microfluidic system to enable the pumping or intermixing of fluids on a microfluidic *** strategy not only enables the assembly of microcomponents but also the combination of different materials and features to form complex hybrid microsystems.

关键词： holographic optical tweezer microassembly microfabrication microfluidics optical assembly screw connection screw-wrench two-photon polymerization

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Simulation of microswimmer hydrodynamics with multiparticle collision dynamics

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Chinese Physics B 2020年第7期29卷 55-61页

作者： andreas Zottl Institute for Theoretical Physics TU WienWiedner Hauptstraße 8-101040 WienAustria

In this review we discuss the recent progress in the simulation of soft active matter systems and in particular the hydrodynamics of microswimmers using the method of multiparticle collision dynamics,which solves the hydrodynamic flows around active objects on a coarse-grained *** first present a brief overview of the basic simulation method and the coupling between microswimmers and *** then review the current achievements in simulating flexible and rigid microswimmers using multiparticle collision dynamics,and briefly conclude and discuss possible future directions.

关键词： active matter multiparticle collision dynamics microswimmers coarse-grained hydrodynamic simulations

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