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Glucose transporter expression in the human colon

World Journal of Gastroenterology 2018年第7期24卷 775-793页

作者： Flavia Merigo Alessandro Brandolese Sonia Facchin Silvia Missaggia Paolo Bernardi Federico Boschi Renata D’Incà Edoardo Vincenzo Savarino andrea sbarbati Giacomo Carlo Sturniolo Department of Neuroscience Biomedicine and Movement Human Anatomy and Histology Section University of Verona Department of Medicine Gastroen-terology Section University of Verona Department of Surgery Oncology and Gastroenterology Gastroenterology Section University Hospital of Padua Department of Computer Science University of Verona

AIM To investigate by immunostaining glucose transporter expression in human colorectal mucosa in controls and patients with inflammatory bowel disease(IBD). METHODS Colorectal samples were obtained from patients undergoing lower endoscopic colonoscopy or rectosigmoidoscopy. Patients diagnosed with ulcerativecolitis(n = 18) or Crohn's disease(n = 10) and scheduled for diagnostic colonoscopy were enrolled. Patients who underwent colonoscopy for prevention screening of colorectal cancer or were followed-up after polypectomy or had a history of lower gastrointestinal symptoms were designated as the control group(CTRL, n = 16). Inflammatory status of the mucosa at the sampling site was evaluated histologically and/or endoscopically. A total of 147 biopsies of colorectal mucosa were collected and processed for immunohistochemistry analysis. The expression of GLUT2, SGLT1, and GLUT5 glucose transporters was investigated using immunoperoxidase labeling. To compare immunoreactivity of GLUT5 and LYVE-1, which is a marker for lymphatic vessel endothelium, doublelabeled confocal microscopy was used. RESULTS Immunohistochemical analysis revealed that GLUT2, SGLT1, and GLUT5 were expressed only in short epithelial portions of the large intestinal mucosa. No important differences were observed in glucose transporter expression between the samples obtained from the different portions of the colorectal tract and between the different patient groups. Unexpectedly, GLUT5 expression was also identified in vessels, mainly concentrated in specific areas where the vessels were clustered. Immunostaining with LYVE-1 and GLUT5 antibodies revealed that GLUT5-immunoreactive(-IR) clusters of vessels were concentrated in areas internal to those that were LYVE-1 positive. GLUT5 and LYVE-1 did not appear to be colocalized but rather showed a close topographical relationship on the endothelium. Based on their LYVE-1 expression, GLUT5-IR vessels were identified as lymphatic. Both inflamed and nonin

关键词： Ulcerative colitis Colon Crohn’s disease Glucose transporter LYVE-1,Immunohistochemistry

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Spontaneous Luminescence Background in Living Nu/Nu Mice

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Advances in Molecular Imaging 2012年第2期2卷 5-11页

作者： Federico Boschi Antonello E Spinelli Laura Calderan andrea sbarbati Department of Neurological Neuropsychological Morphological and Motor Sciences Section of Anatomy and Histology University of Verona Verona Italy Medical Physics Department S. Raffaele Scientific Institute Milan Italy

Spontaneous light emission from living animals can overcome the investigated light signals in small animal luminescence imaging. Despite autofluorescence emission is well studied the spontaneous luminescence background is less known and its importance is growing due to the new born imaging techniques like Cerenkov Luminescence Imaging and Radionuclide Luminescence Imaging in which faint sources are often involved. In order to investigate the spontaneous emission we studied the background luminescence in vivo from health Nu/Nu mice in optical imaging acquisitions and we related it with the optical properties of the diet of the animals. In particular luminescence images of mice feed with normal diet used in animal facilities were acquired using a commercial optical imager. The intensity and the spectral features of the luminescence emission from the animal surface after sunshine exposition and after normal lighting laboratory conditions were measured. The same was done with the pellets of food used to feed the animals. We found a background emission from the entire animal surface and localized light sources in the abdominal/lumbar region. Their intensity can be modulated by the light exposition of the animals before the imaging session and decreases along the time when they are put in darkness. The comparison of the luminescence time decay of animals and pellets suggests that the light sources are related to the persistent luminescence of the molecules contained in the food. So ambient exposure before imaging is important for luminescence imaging in order to keep down the background. The optical properties of food are also important and it necessary to check them before to feed the animals not only in fluorescence imaging but also in luminescence imaging.

关键词： In Vivo Small Animal Imaging Optical Imaging Bioluminescence Cerenkov Luminescence Imaging Radionuclide Luminescence Imaging Diet

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