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Soybean Ferritin:Isolation,Characterization,and Free Radical Generation

Journal of Integrative Plant Biology 2012年第1期54卷 45-54页

作者： andrea galatro Elizabeth Robello Susana Puntarulo Physical Chemistry-PRALIB School of Pharmacy and BiochemistryUniversity of Buenos Aires

The main aim of this work was to assess the multi-task role of ferritin （Ft） in the oxidative metabolism of soybean （Glycine max）. Soybean seeds incubated for 24 h yielded 41 4- 5μg Ft/g fresh weight. The rate of in vitro incorporation of iron （Fe） into Ft was tested by supplementing the reaction medium with physiological Fe chelators. The control rate, observed in the presence of 100μM Fe, was not significantly different from the values observed in the presence of 100 μM Fe-his. However, it was significantly higher in the presence of 100 μM Fe-citrate （approximately 4.5-fold） or of 100 μM Fe-ATP （approximately 14-fold）. Moreover, a substantial decrease in the Trp-dependent fluorescence of the Ft protein was determined during Fe uptake from Fe-citrate, as compared with the control. On the other hand, Ft addition to homogenates from soybean embryonic axes reduced endogenously generated ascorbyl radical, according to its capacity for Fe uptake. The data presented here suggest that Ft could be involved in the generation of free radicals, such as hydroxyl radical, by Fe-catalyzed reactions. Moreover, the scavenging of these radicals by Ft itself could then lead to protein damage. However, Ft could also prevent cellular damage by the uptake of catalytically active Fe.

关键词： ferritin free radical iron soybean.

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The complexity of nitric oxide generation and function in plants

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BIOCELL 2016年第1期40卷 1-5页

作者：无 MARIA P BENAVIDES SUSANA M GALLEGO FACUNDO RAMOS ARTUSO MARIANA CHECOVICH andrea galatro Instituto de Bioquímica y Medicina Molecular(IBIMOL) Universidad de Buenos Aires-CONICETBuenos AiresArgentina Cátedra Química Biológica Vegetal Instituto de Química y Fisicoquímica Biológicas"Prof.Alejandro C.Paladini"(IQUIFIB)UBA-CONICETBuenos AiresArgentina Instituto de Fisiología Vegetal(INFIVE)CONICET-UNLP La PlataArgentina Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús(IIB-INTECH) CONICET-UNSAMChascomúsArgentina

Plants are exposed to environmental stress,in natural and agricultural *** oxide(NO),a small gaseous molecule which plays important roles in plants,has been involved in many physiological processes,and emerged as an important endogenous signaling molecule in the adaptation of plants to biotic and abiotic *** is produced from a variety of enzymatic and non enzymatic sources,which are not yet fully ***,NO and reactive nitrogen species(RNS)can produce posttranslational modifications affecting protein *** reductase,a key enzyme in the nitrogen metabolism,is a proposed source of NO in plants which could be affected by posttranslational ***,different pathways seem to be involved and can also regulate NO synthesis in the plant cell under physiological or stress ***,how the levels of NO are reached in such time and place to fulfill its functions,are still puzzles to elucidate.

关键词： nitrate reductase polyamines posttranslational modifications

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