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Genetic Relationships between Cultivated and Wild Olive Trees (Olea Europaea L. Var. Europaea and Var. Sylvestris) Based on Nuclear and Chloroplast SSR Markers

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Natural Resources 2010年第2期1卷 95-103页

作者： Hedia Hannachi Catherine Breton Monji Msallem Salem Ben El Hadj Mohamed El Gazzah andre berville 不详

The olive is widely cropped in Tunisia where also oleaster trees thrive all around orchards and in natural sites. Little is known on the genetic relationships between the olive crop and oleaster trees in Tunisia. Fifty-two oleaster trees and fifteen cultivars were sampled from Tunisia. SSR genotyping was performed in polyacrylamide gels after fluorescent labeling. We used seven nuclear and two chloroplast SSR markers. AFC analyses showed close genetic relationships between cultivated and oleaster trees. Genetic relationships were also displayed in a dendrogram based on Unweighted Pair Group Method (UPGMA). Five clusters were defined mixing cultivar and oleaster trees suggesting close relationship between some cultivar and some oleaster trees. One oleaster is single in a cluster. The chlorotype SSR markers show probably three olive origins. Some cultivars have the CE chlorotype originates from the East of the Mediterranean basin, the CCK haplotype originates from Maghreb and the COM chlorotype originates from West Mediterranean. The cultivars were 1) introduced from the East;2) selected in the West;3) or selected in the North Africa region. The Tunisian oleaster trees carry eastern and western Mediterranean chlorotype CCK, COM and CE.

关键词： Cultivars Oleaster Genetic Relationship SSR Markers Haplotype Origin

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Molecular markers of nuclear restoration gene Rf1 in sunflower using bulked segregant analysis-RAPD

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Science China(Life Sciences) 1996年第5期39卷 551-560页

作者：季静王罡 E.Belhassen H.Serieys A.berville Laboratory of Paant Molecular Biology Changchun University of Agricultural and Animal Sciences. Changchun 130062 China INRA Station de Genetique et d'Amelioration des Plantes 2 Place de Viala Chinese de Phytotechnie34060 Nontpellier France

Restoration of cytoplasmic male sterility (CMS) in sunflower was demonstrated to be controlled by polygenes by analysing 982 effective crosses among 109 self-crossed lines and 16 CMS lines. Two self-crossed lines and one CMS line with distinct genotypes were applied to creation of segregating populations for DNA bulks of the target gene Rfl. Bulked DNA was prepared in order to investigate single gene Rfl and its gene marker among polygenic characters at the same genetic background. Using 80 10-mer operon primers, 620 RAPD reactions were carried out between fertile and sterile DNA bulks. In about 800 loci, primary results showed that 8 were related to the restoration genes. Furthermore. 2 were confirmed as RAPD markers for gene Rfl by examining 9 maintenance and 7 restoration lines. This method is the improvement for bulked segregant analysis[1] with which markers of single gene of target can be identified rapidly among polygenic characters.

关键词： sunflower cytoplasmic male sterility restoration gene bulked DNA RAPD marker.

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5%米喹莫特霜剂治疗12例未分化外阴上皮内肿瘤的前瞻性研究

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世界核心医学期刊文摘（皮肤病学分册） 2005年第2期1卷 9-9页

作者： Wendling J. Saiag P. berville-Levy S. M.Moyal-Barracco 罗素菊冯义国 Serv. Dermatol. Gen. et Oncologique H.opital Ambroise-Par'e 9 Avenue Charles-de-Gaulle 92104 Boulogne Cedex France Dr.

Objective: To assess the efficacy of 5%imiquimod cream on undifferentiated vulvar intraepithelial neoplasia (VIN), a disease caused by high-risk human papillomavirus. Design: Prospective, uncontrolled study. Setting: University hospital vulvar clinic. Patients: Twelve consecutive patients treated with 5%imiquimod cream for undifferentiated VIN between March 1, 1999, and May 31, 2001. Intervention: Self-application of 5%imiquimod cream, initially 3 times a week, then adjusted according to tolerance, for up to 7 months according to clinical response. Main Outcome Measures: Therapeutic response, clinically assessed by successive photographs and histologically confirmed for complete responders, was scored as complete, partial (≥50%decrease in lesion size), or failure. Tolerance was evaluated at each visit. Results: A total of 3, 4, and 5 patients achieved complete response, partial response (≥75%reduction in lesion size for all such cases), and failure, respectively. Mean duration of treatment was 3.6 months (37.3 applications), 5.0 months (50.7 applications), and 3.4 months (25.2 applications) for complete responders, partial responders, and failures, respectively. Follow-up after treatment was 5 to 18, 14 to 32, and 2 to 28 months, respectively, with 1 partial responder lost to long-term follow-up. No patient developed invasive carcinoma. All but 2 patients experienced vulvar discomfort, resulting in treatment withdrawal for 3. Two patients had flulike symptoms. Conclusions: Imiquimod cream could be a therapeutic option for undifferentiated VIN. Although poorly tolerated, this self-applied treatment could spare patients, either totally or partially, the classic painful and sometimesmutilating treatments of VIN. Controlled, randomized studies are needed to evaluate its efficacy and tolerance.

关键词：外阴上皮内肿瘤莫特霜剂人类乳头瘤病毒组织学检查临床反应用药次数侵袭性癌流感样症状大学医院

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