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The Optimization of Aspergillus ***4 Tannase Production under Submerged Fermentation

Advances in Microbiology 2014年第3期4卷 143-150页

作者： Alessandra Goncalves de Melo Rayssa Cristina Faria Pedroso Luis Henrique Souza Guimaraes José Guilherme Lembi Ferreira Alves Eustáquio Souza Dias Mário Lúcio Vilela de Resende patrícia gomes Cardoso Department of Biology Sector of MicrobiologyFederal University of LavrasLavrasBrazil Department of Biology University of Sao PauloRibeirao PretoBrazil Department of Food Science Federal University of LavrasLavrasBrazil Department of Phytopathology Federal University of LavrasLavrasBrazil

Tannase is a hydrolytic enzyme that is involved in the biodegradation of tannins and it has biotechnological potential in the pharmaceutical, chemical, food and beverage industries. Microorganisms, especially filamentous fungi, are important tannase producers. The aims of this work were to find a potential tannase producer and to improve the cultivation conditions. Three Aspergillus species (A. japonicus 246A, A. tamarii 3 and Aspergillus sp. GM4) were investigated in different culture media (Adams, Czapeck, Khanna, M5 and Vogel) and inducers (1% and 2% tannic acid;1% green tea;1% methyl gallate;1% gallic acid). Aspergillus sp. GM4 and Adams medium were selected. The tannase production by Aspergillus sp. GM4 in Adams medium was induced in the presence of 2% (w/v) tannic acid and gallic acid as carbon sources, while green tea was not able to induce tannase production. The Plackett-Burman screening design was performed with the variables MgSO4, KH2PO4, yeast extract, tannic acid, agitation rate and salt solution. The variables MgSO4 and agitation rate were selected for the optimization of tannase production using a Central Composite Rotatable Design. Under optimized conditions, a 2.66-fold increase in the enzyme production was observed with small modifications in the medium composition.

关键词： Aspergillus sp. Response Surface Methodology Submerged Fermentation Tannase Tannic Acid

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Hypertonic saline:a brief overview of hemodynamic response and anti-inflammatory properties in head injury

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Neural Regeneration Research 2015年第12期10卷 1938-1939页

作者： Matheus Fernandes de Oliveira Fernando Campos gomes Pinto Department of Neurosurgery Hospital do Servidor Público Estadual de S?o PauloIAMSPE Division of Functional Neurosurgery of the Institute of Psychiatry Hospital das ClínicasUniversidade de S?o Paulo

Hypertonic saline （HS） has been applied in several med- ical areas such as pneumology （asthma, cystic fibrosis and bronchiolytis）, endocrinology （hyponatremia） and especially in emergency medicine, in traumatic and in- flammatory/infectious disorders. It may be composed of 3% or 7.5% sodium chlorate. By far, 3% solution is the most widely studied and used solution （Pinto et al., 2006, 2015; Dekker et al., 2014; Gantner et al., 2014; Shein et al., 2014）.

关键词： TBI Hypertonic saline head

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Yield and Chemical Composition of Brachiaria Forage Grasses in the Offseason after Corn Harvest

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American Journal of Plant Sciences 2014年第7期5卷 933-941页

作者： Gean Alves Maia Kátia Aparecida de Pinho Costa Eduardo da Costa Severiano patrícia Soares Epifanio José Flávio Neto Matheus Goncalves Ribeiro patrick Bezerra Fernandes José Fausto Guimaraes Silva Wainer gomes Goncalves Laboratory of Soil Physics Federal Institute GoianoRio VerdeBrazil Laboratory of Forage and Grassland Federal Institute GoianoRio VerdeBrazil

This study evaluated the dry matter production and chemical composition of Brachiaria forage grasses in the offseason after corn harvest in integrated crop-livestock system. The experiment was conducted at the Federal Institute of Goiás, Rio Verde Campus, using a randomized complete block experimental design, with four replications. The treatments consisted of the forages: Brachiaria brizantha cv. Marandu;Brachiaria brizantha cv. Xaraes;Brachiaria brizantha cv. Piata;Brachiaria brizantha cv. MG-4;Brachiaria decumbens and Brachiaria ruziziensis, intercropped in oversown corn for implantation of integrated crop-livestock system. The results showed that intercropping corn with Brachiaria grasses favors the production of high-quality forage in the offseason, and the cultivars of Brachiaria brizantha and Brachiaria decumbens showed higher dry matter production. And cultivars of Brachiaria brizantha (Marandu palisadegrass, Xaraes palisadegrass and Piata palisadegrass) are the most suitable for presenting food of better quality, compared with Brachiaria brizantha cv. MG-4, Brachiaria decumbens and Brachiaria ruziziensis.

关键词： Brachiaria brizantha Brachiaria decumbens Brachiaria ruziziensis Crop-Livestock Integration

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Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli

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Asian Pacific Journal of Tropical Medicine 2022年第2期15卷 71-78页

作者： Guilherme S.Machado Rosane L.Lopes Simone S.C.Oliveira Simone P.C.Freitas Jacenir R.Santos-Mallet andréL.S.Santos Denise Feder Suzete A.O.gomes Programa de Pós-Graduação em Ciências e Biotecnologia Instituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Biodiversidade de Insetos e Patógenos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes Departamento de Microbiologia GeralInstituto de Microbiologia Paulo de GóesUniversidade Federal do Rio de JaneiroRJBrazil Fundação Oswaldo Cruz Piauí TeresinaPIBrazil Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera Instituto Oswaldo CruzFundação Oswaldo CruzRio de JaneiroRJBrazil Universidade Iguaçu Nova IguaçuRJBrazil Laboratório de Biologia de Insetos Departamento de Biologia GeralInstituto de BiologiaUniversidade Federal FluminenseNiteróiRJBrazil

Objective:To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies(sites of vitellogenesis)from Rhodnius(R.)prolixus infected with Trypanosoma(T.)***:Adult virgin females of *** were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of *** epimastigotes(Macias strain).Females from the control group and all the males received parasite-free *** electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected *** profile,proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected ***:According to the ultrastructural data,*** infection elicited a degeneration process in the ovarian follicular cells of *** assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group,while a significant increase in the cysteine peptidase activity was measured in both insect ***,immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected ***:*** infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of ***.

关键词： Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease

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Neurogenesis in the hippocampus of adult humans: controversy “fixed” at last

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Neural Regeneration Research 2019年第11期14卷 1917-1918页

作者： Silene M.A. Lima Walace gomes-Leal Laboratory of Neurobiology Institute of Biological SciencesFederal University of ParaBelem-ParaBrazil Laboratory of Experimental Neuroprotection and Neuroregeneration Institute of Biological SciencesFederal University of ParaBelem-ParaBrazil

The presence of adult neurogenesis in the mammalian brain has been a theme of intense controversy for a long time since the original report by Altman and Das (1965). The scientific community, for about 30 years, has difficulties to accept that progenitor cells give rise to new neurons in some specific regions of the mammalian adult brain, the neurogenic niches (Kuhn et al., 1996;Doetsch et al., 1997, 1999).

关键词： Neurogenesis hippocampus mammalian brain

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Combination approaches in hepatocellular carcinoma:How systemic treatment can benefit candidates to locoregional modalities

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World Journal of Gastroenterology 2022年第28期28卷 3573-3585页

作者： Leonardo gomes da Fonseca Raphael L C Araujo Department of Medical Oncology Instituto do Cancer do Estado de Sao PauloUniversity of Sao PauloSão Paulo 01246-000São PauloBrazil Hospital e Maternidade Brasil-Rede D'Or São Luiz Santo André09030-590São PauloBrazil Department of Digestive Surgery Escola Paulista de Medicina-UNIFESPSão Paulo 04023-062São PauloBrazil

The management of hepatocellular carcinoma(HCC)is challenging because most patients have underlying cirrhosis,and the treatment provides,historically,a limited impact on the natural history of patients with advanced-stage ***,recurrence rates are high for those patients who receive local and locoregional modalities,such as surgical(resection and transplantation)or imageguided(ablation and intra-arterial)*** research has led to new concepts that are reshaping the current clinical *** advancements were achieved in the understanding of the hallmarks that drive *** has primed a successful incorporation of novel agents with different targets,such as anti-angiogenic drugs,targeted-therapies,and immune-checkpoint *** clinical trials have proven efficacy of systemic agents in advanced stage disease,there is no conclusive evidence to support their use in combination with loco-regional *** novel local modalities are being incorporated(e.g.,radioembolization,microwave ablation,and irreversible electroporation),emerging data indicate that locoregional treatments may induce tumor microenvironment changes,such as hyperexpression of growth factors,release of tumor antigens,infiltration of cytotoxic lymphocytes,and modulation of adaptative and innate immune *** trials that evaluated the use of antiangiogenic drugs in the adjuvant setting after ablation or chemoembolization fail to demonstrate a substantial *** efforts are directed to investigate the role of immunotherapy-based regimens in this *** present review aims to describe the current landscape of systemic and locoregional treatments for HCC,present evidence to support combination approaches,and address future perspectives.

关键词： Liver cancer Hepatocellular carcinoma Immunotherapy Systemic therapy Ablation Embolization

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Engineering carbon quantum dots for photomediated theranostics

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Nano Research 2018年第1期11卷 1-41页

作者： Mahbub Hassan Vincent G. gomes Alireza Dehghani Sara M. Ardekani School of Chemical and Biomolecular Engineering The University of Sydney NSW 2006 Australia

Carbon quantum dots （CQDs） have emerged as potential alternatives to classical metal-based semiconductor quantum dots （QDs） due to the abundance of their precursors, their ease of synthesis, high biocompatibility, low cost, and particularly their strong photoresponsiveness, tunability, and stability. Light is a versatile, tunable stimulus that can provide spatiotemporal control. Its interaction with CQDs elicits interesting responses such as wavelength-dependent optical emissions, charge/electron transfer, and heat generation, processes that are suitable for a range of photomediated bioapplications. The carbogenic core and surface characteristics of CQDs can be tuned through versatile engineering strategies to endow specific optical and physicochemical properties, while conjugation with specific moieties can enable the design of targeted probes. Fundamental approaches to tune the responses of CQDs to photo-interactions and the design of bionanoprobes are presented, which enable biomedical applications involving diagnostics and therapeutics. These strategies represent comprehensive platforms for engineering multifunctional probes for nanomedicine, and the design of QD probes with a range of metal-free and emer^in~ 2D materials.

关键词： carbon quantum dots nanobiophotonics therapeutics diagnostics nanomedicine

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Contact Precaution: Acceptance of Health Professionals to Teaching Hospital

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Open Journal of Nursing 2020年第5期10卷 551-562页

作者： Hercília Oliveira dos Santos Marli de Carvalho Jericó Viviane Decicera Colombo Oliveira Geraldo Magela de Faria Junior patrícia de Carvalho Jericó Pedro Paulo de Carvalho Jericó Ingrid gomes Campos Truzzi Angela Silveira Gagliardo Calil Faculdade de Medicina de São José do Rio Preto (FAMERP) São José do Rio Preto Brazil Pontifícia Universidade Católica do Rio Grande do Sul Porto Alegre Brazil Pontifícia Universidade Católica do Paraná Curitiba Brazil

The adoption by health professionals to the practice of patient isolation is a decision that reduces patients’ risks of occupational exposure to potentially contaminated biological material and prevents nosocomial infections. Verify the compliance of health professionals to the practices of contact precautions in patients colonized by microorganisms in a teaching hospital. This was a prospective observational cohort study in a special-sized hospital located in southeastern Brazil. The observation of health professionals in delivery of health care to patients with contact precaution was performed regarding hand hygiene, use of overcoat, glove, and mask. We carried out 1502 observations involving the following professional categories: nursing technicians and/or nursing assistants (n = 1028;68.4%), nurses (n = 200;13.3%), physical therapists (n = 185;12.3%), and physicians (n = 89;5.9%), totaling 971.8 hours. Regarding the level of compliance of professionals to contact precaution practices with hand hygiene after the procedures, the use of overcoats, gloves, and masks, all were statistically significant (p Klebsiella pneumoniae was the most positive pathogen identified in laboratory tests. This study provided the mapping of the compliance of health professionals to the practices of contact precautions in order to support a safer management of patient care reducing the risks of Healthcare-Associated Infections (HAI). This study also showed that health professionals are using practice management to deal with their behavior to protect their health.

关键词： Universal Precautions Infection Control Services Hospital Patient Isolation Drug Resistance Microbial Quality Indicators Health Care Process Assessment (Health Care)

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Pancreatic paracoccidioidomycosis simulating malignant neoplasia:Case report

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World Journal of Gastroenterology 2013年第34期19卷 5750-5753页

作者： Talles Bazeia Lima Maria Aparecida Custódio Domingues Carlos Antonio Caramori Giovanni Faria Silva Cássio Vieira de Oliveira Fábio da Silva Yamashiro Letícia de Campos Franzoni Lígia Yukie Sassaki Fernando gomes Romeiro Division of Gastroenterology Department of Internal MedicineBotucatu School of MedicineRubio JuniorDistrict S/N18618-970 So PauloBrazil Department of Pathology Botucatu School of MedicineRubio Junior District S/N18618-970 So PauloBrazil

Paracoccidioidomycosis is a systemic granulomatous disease caused by fungus,and must be considered in the differential diagnosis of intra-abdominal tumors in endemic *** report a rare case of paracoccidioidomycosis in the pancreas.A 45-year-old man was referred to our institution with a 2-mo history of epigastric abdominal pain that was not diet-related,with night sweating,inappetence,weight loss,jaundice,pruritus,choluria,and acholic feces,without signs of sepsis or palpable *** ultrasonography(US)showed a solid mass of approximately 7 cm×5.5 cm on the pancreas *** computerized tomography showed dilation of the biliary tract,an enlarged pancreas(up to 4.5 in the head region),with dilation of the major pancreatic *** patient underwent exploratory laparotomy,and the surgical description consisted of a tumor,measuring 7 to 8 cm with a poorly-defined margin,adhering to posterior planes and mesenteric vessels,showing an enlarged bile *** drainage of the biliary tract,Rouxen-Y gastroenteroanastomosis,lymph node excision,and biopsies were performed,but malignant neoplasia was not *** analysis showed chronic pancreatitis and a granulomatous chronic inflammatory process in the choledochal lymph ***-alcohol resistant bacillus and fungus screening were ***-needle aspiration of the pancreas was performed under US *** smear was compatible with infection by Paracoccidioides *** report a rare case of paracoccidioidomycosis simulating a malignant neoplasia in the pancreas head.

关键词： Paracoccidioidomycosis Pancreas Fungus infection Pancreatic tumors Differential diagnosis

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Influence of train speed and its mitigation measures in the short-and long-term performance of a ballastless transition zone

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Railway Engineering Science 2023年第4期31卷 309-324页

作者： Ana Ramos Rui Calçada António gomes Correia Department of Civil Engineering Faculty of EngineeringUniversity of PortoCONSTRUCT-LESEPortoPortugal Department of Civil Engineering University of MinhoISISEGuimarãesPortugal

The ballastless track is nowadays the most popular railway system due to the required low number of maintenance opera-tions and costs,despite the high *** gradual change from ballasted to ballastless tracks has been occurring in Asia,but also in Europe,increasing the number of transition *** transition zones are a special area of the railway networks where there is an accelerated process of track degradation,which is a major concern of the railway infrastructure ***,the accurate prediction of the short-and long-term performance of ballastless tracks in transition zones is an important topic in the current paradigm of building/rehabilitating high-speed *** work purposes the development of an advanced 3D model to study the global performance of a ballastless track in an embankment-tunnel transition zone considering the influence of the train speed(220,360,500,and 600 km/h).Moreover,a mitigation measure is also adopted to reduce the stress and displacements levels of the track in the transition.A resilient mat placed in the tunnel and embank-ment aims to soften the *** behaviour of the track with the resilient mat is evaluated considering the influence of the train speed,with special attention regarding the critical *** used methodology is a novel and hybrid approach that allows including short-term and long-term performance,through the development of a powerful 3D model combined with the implementation of a calibrated empirical permanent deformation model.

关键词： Ballastless track Train speed Numerical modelling Transition zone Critical speed

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