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Diversity among Isolates of the Tangerine Pathotype of Alternaria alternata

American Journal of Plant Sciences 2018年第2期9卷 196-215页

作者： andréa celina ferreira demartelaere Luciana Cordeiro do Nascimento Cristina O.Marinho Marciano Costa Nunes Patrícia Clemente Abraao Rommel S.S.Gomes Monica Danielly de Mello Oliveira Larissa Almeida Mirelly M.Porcino Caroline Vargas e Silva Wilza C.O.Souza Programa de Pós-Graduacao em Agronomia Departamento de Fitotecnia e Ciencias AmbientaisUniversidade Federal da ParaíbaAreiaBrazil

The tangerine pathotype of Alternaria alternata is the aetiological agent of Alternaria brown spot on tangerines. In the state of Paraíba, Brazil, its occurrence on “Dancy” tangerine trees is associated with genetic aspects as well as the influence of environmental conditions on reproduction and dissemination within and between populations. The aim of the present study was to evaluate the diversity of isolates of this pathogen using morphophysiological and molecular markers. For the analysis of mycelial growth and sporulation, 30 isolates from different locations were examined at 24-hour intervals until the seventh day, when the spores were quantified. The 30 isolates were characterised based on molecular markers (ISSR) and genetic similarity (Jaccard index). A factor arrangement was used: 30 isolates, four media (ODA, PDA, LEA and V8), three light regimes (continuous dark, alternating light and continuous light) and three temperatures (15°C, 25°C and 35°C), with 12 repetitions. Groups 1, 2 and 3 presented low genetic variability. Group 4 showed high genetic variability of the isolates obtained from the Massaranduba (state of Paraíba-Brazil) producing region and higher mycelial growth and sporulation of A. alternata. The continuous light regime and the temperature 25°C in PDA and V8 media were the ideal conditions for the mycelial growth and sporulation, respectively, of the isolates of A. alternata.

关键词： Citrus tangerine Alternaria Brown Spot Fungal Variability Environment Factors ISSR Markers

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Application of data science in the prediction of solar energy for the Amazon basin:a study case

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Clean Energy 2023年第6期7卷 1344-1355页

作者： andréLuis ferreira Marques Márcio JoséTeixeira Felipe Valencia de Almeida Pedro Luiz Pizzigatti Corrêa Polytechnic School of the University of Sao Paulo Av.Prof.Luciano Gualberto380-ButantaSao Paulo-SP05508-010Brazil Institute of Physics of the University of Sao Paulo Brazil

The need for renewable energy sources has challenged most countries to comply with environmental protection actions and to handle climate *** energy figures as a natural option,despite its *** has a green energy matrix with significant expansion of solar form in recent *** preserve the Amazon basin,the use of solar energy can help communities and cities improve their living standards without new hydroelectric units or even to burn biomass,avoiding harsh environmental *** novelty of this work is using data science with machine-learning tools to predict the solar incidence(W.h/m^(2))in four cities in Amazonas state(north-west Brazil),using data from NASA satellites within the period of ***-tree-based models and vector autoregressive(time-series)models were used with three time aggregations:day,week and *** predictor model can aid in the economic assessment of solar energy in the Amazon basin and the use of satellite data was encouraged by the lack of data from ground *** mean absolute error was selected as the output indicator,with the lowest values obtained close to 0.20,from the adaptive boosting and light gradient boosting algorithms,in the same order of magnitude of similar references.

关键词： solar energy renewable energy Amazon basin machine learning time series data science decision-trees ensemble vector autoregression

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Improvement in varistor properties of CaCu_(3)Ti_(1)O_(12) ceramics by chromium addition

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Journal of Materials Science & Technology 2020年第6期41卷 12-20页

作者： Edson Cezar Grzebielucka Joao Frederico Haas Leandro Monteiro Eder Carlos ferreira de Souza Christiane Philippini ferreira Borges andréVitor Chaves de andrade Eloísa Cordoncillo Héctor Beltrán-Mir Sandra Regina Masetto Antunes Programa Pós-Graduacao em Química Aplicada Universidade Estadual de Ponta GrossaAv.Gen.Carlos Cavalcanti 474884.030-000Ponta GrossaParanáBrazil Programa de Pós-Graduacao em Ciências/Física Universidade Estadual de Ponta GrossaAv.Gen.Carlos Cavalcanti 474884.030-000Ponta GrossaParanáBrazil Dpto.de Química Inorgánica y Orgánica Universitat Jaume IAv.de Vicent Sos Baynats/n12071Castellón de la PlanaSpain

The effect of chromium addition on the structure and electrical properties of CaCu3Ti4O12 is *** based on the formula CaCu3Ti4-xCrxO12-σ(x = 0, 0.025, 0.050 and 0.075 mol%) are prepared by solid-state reaction. Pellets sintered at 1070℃ for 12 h are characterised by X-ray diffraction, scanning electron microscopy/energy dispersive spectroscopy and impedance spectroscopy. Current densityelectric field, dielectric loss and permittivity measurements are also carried out. Rietveld refinement showed the substitution of Cr(Ⅲ) on the Ti(Ⅳ) site, with the maximum substitution for x = 0.025 mol%.The generation of electrical defects in the grain boundary region by chromium doping is responsible for increasing the electric breakdown field strength and nonlinearity coefficient at room temperature from 1723 V/cm and 5.29 for x = 0 mol% to 3431 V/cm and 8.16 for x = 0.025 mol%. This composition shows the greatest improvement in ceramic varistor parameters.

关键词： CCTO Structural analysis Impedance Varistor Rietveld

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Glycoproteogenomics: Setting the Course for Next-generation Cancer Neoantigen Discovery for Cancer Vaccines

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Genomics, Proteomics & Bioinformatics 2021年第1期19卷 25-43页

作者： José Alexandre ferreira Marta Relvas-Santos andreia Peixoto andréM.N.Silva Lúcio Lara Santos Experimental Pathology and Therapeutics Group Portuguese Institute of OncologyPorto 4200-072Portugal Institute of Biomedical Sciences Abel Salazar University of PortoPorto 4050-313Portugal Porto Comprehensive Cancer Center(P.ccc) Porto 4200-072Portugal REQUIMTE-LAQV Department of Chemistry and BiochemistryFaculty of Sciences of the University of PortoPorto 4169-007Portugal

Molecular-assisted precision oncology gained tremendous ground with high-throughput next-generation sequencing(NGS),supported by robust *** quest for genomicsbased cancer medicine set the foundations for improved patient stratification,while unveiling a wide array of neoantigens for *** pre-clinical and clinical studies have successfully used tumor-specific peptides in vaccines with minimal off-target ***,the low mutational burden presented by many lesions challenges the generalization of these solutions,requiring the diversification of neoantigen *** utilizing customized databases for protein annotation by mass spectrometry(MS)is a powerful tool toward this *** the concept toward exploring proteoforms originated from post-translational modifications(PTMs)will be decisive to improve molecular subtyping and provide potentially targetable functional nodes with increased cancer *** through the path of systems biology,we highlight that alterations in protein glycosylation at the cell surface not only have functional impact on cancer progression and dissemination but also originate unique molecular fingerprints for targeted ***,we discuss the outstanding challenges required to accommodate glycoproteomics in oncoproteogenomics *** envisage that such rationale may flag a rather neglected research field,generating novel paradigms for precision oncology and immunotherapy.

关键词： Glycoproteogenomics Oncoproteogenomics Cancer neoantigens Glycosylation Precision oncology

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First Insights into the Viral Communities of the Deep-sea Anoxic Brines of the Red Sea

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Genomics, Proteomics & Bioinformatics 2015年第5期13卷 304-309页

作者： andr Antunes Intikhab Alam Marta Filipa Simoes Camille Daniels Ari J.S.ferreira Rania Siam Hamza El-Dorry Vladimir B.Bajic Computational Bioscience Research Center (CBRC) ComputerElectrical and Mathematical Sciences and Engineering Division (CEMSE)King Abdullah University of Science and Technology (KAUST) Red Sea Research Center (RSRC) Biological and Environmental Sciences and Engineering Division (BESE)King Abdullah University of Science and Technology (KAUST) Biology Department American University in Cairo

The deep-sea brines of the Red Sea include some of the most extreme and unique envi- ronments on Earth. They combine high salinities with increases in temperature, heavy metals, hydrostatic pressure, and anoxic conditions, creating unique settings for thriving populations of novel extremophiles. Despite a recent increase of studies focusing on these unusual biotopes, their viral communities remain unexplored. The current survey explores four metagenomic datasets obtained from different brine-seawater interface samples, focusing specifically on the diversity of their viral communities. Data analysis confirmed that the particle-attached viral communities present in the brine-seawater interfaces were diverse and generally dominated by Candovirales, yet appearing distinct from sample to sample. With a level of caution, we report the unexpected finding of Phycodnaviridae, which infects algae and plants, and trace amounts of insect-infecting Iridoviridae. Results from Kebrit Deep revealed stratification in the viral communities present in the interface： the upper-interface was enriched with viruses associated with typical marine bacteria, while the lower-interface was enriched with haloviruses and halophages. These results provide first insights into the unexplored viral communities present in deep-sea brines of the Red Sea, represent- ing one of the first steps for ongoing and future sampling efforts and studies.

关键词： Viral diversity Metagenomics Brine-seawater interface Caudovirales Red Sea

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Biochar influences the cane fields’microbiota and the development of pre-sprouted sugarcane seedlings

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Waste Disposal and Sustainable Energy 2023年第1期5卷 75-88页

作者： Bruna Cristina Pinto Adriana Barboza Alves Osania Emerenciano ferreira Gustavo Henrique Gravatim Costa Magno andréde Oliveira Augusto Cesar da Silva Bezerra Alan Rodrigues Teixeira Machado Postgraduate Program in Environmental Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38202-436Brazil Department of Agricultural and Biological Sciences State University of Minas GeraisFrutal UnityFrutalMinas Gerais38208-436Brazil Postgraduate Program in Geotechnical Engineering Federal University of Ouro PretoOuro PretoMinas Gerais35400-000Brazil Department of Transport Engineering Federal Center for Technological Education of Minas GeraisBelo HorizonteMinas Gerais30421-169Brazil Department of Exact Sciences State University of Minas GeraisJoão Monlevade UnityJoão MonlevadeMinas Gerais35930-314Brazil

This research investigates the soil conditioning effect of sugar cane fields with biochar produced from sugarcane *** influence on the physicochemical and microbiological conditions of the soil and the agronomic performance of sugarcane seedlings was *** this end,the biochar from sugarcane bagasse was produced by pyrolysis in a double drum oven and mixed with soil at rates of 0,1%,3%,and 5%(in weight).The experiment was installed in a greenhouse with two evaluation periods(30 days and 60 days)and five *** pot received a pre-sprouted cane seedling,and the analysis of colony-forming units(bacteria and fungi),physicochemical characteristics of the soil,and growth and development of sugarcane were performed per *** biochar showed a high fixed carbon content(72%)and a specific surface area estimated by the adsorption of methylene blue of 50 m2 g−***,its structure is porous and contains important nutrients(e.g.,CaO,K2O,and P2O5).In the periods evaluated,the applications of the 3%and 5%(in weight)rates of biochar reduced most of the soil fertility ***,the chemical analyses indicated that the sugarcane field soil collected already possessed high *** microbiota was influenced,but only the application of the 3%(in weight)rate at 60 days after planting showed a significant positive effect on the number of bacteria forming units,with an increase of approximately 385%.On the other hand,no significant positive effect on sugarcane development was ***,biochar application in high-fertility sugarcane plantation soil did not result in gains for sugarcane development in the periods evaluated.

关键词： Soil Biometry Microbiology Double drum oven Biochar

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Demodulating the Response of Optical Fibre Long-Period Gratings： Genetic Algorithm Approach

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Chinese Physics Letters 2006年第9期23卷 2480-2482页

作者： P. S. andré R. A. Sá. ferreira C. M. L. Correia H. Kalinowshy 忻向军 J.L.Pinto Instituto de Telecomunicaoes Universidade de A veiro Campus de Santiago 3810-193 A veiro Portugal Departamento de Fisica Universidade de A veiro Campus de Santiago 3810-193 A veiro Portugal Centro de Investigacao em Materiais Compositos e Cergmicos CICECO Universidade de A veiro Campus de Santiago 3810-193 A veiro Portugal Centro Federal de Educao Tecnologica do Parana A v. Sere de Setembro 3165 80230-901 Curitiba-PR Brasil School of Electronic Engineering Beijing University of Posts and Telecommunications Beijing 100876

The extraction of the physical parameters of long period gratings from the spectral response is not an easy process. We present a demodulation technique to synthesize the physical parameters of a long period grating recorded in an optical fibre. The demodulation is achieved through the implementation of a genetic algorithm. The extracted parameters are in agreement with the typical values known for long period gratings.

关键词： SENSORS INDEX

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H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression

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World Journal of Gastroenterology 2008年第6期14卷 884-891页

作者： Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa andré Márcia Valéria Pitombeira ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst Departament of Pathology and Forensic Federal University of Ceará State Brazil Saint House of Mercy in Fortaleza Brazil

AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents.

关键词： Gastric carcinoma Helicobacter pylori Epstein-Barr virus, p53, Bax, Bcl-2, c-Myc

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Association of nonalcoholic fatty liver disease and liver cancer

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World Journal of Gastroenterology 2015年第3期21卷 913-918页

作者： Perla Oliveira Schulz Fabio Goncalves ferreira Maria de Fátima Araújo Nascimento andrea Vieira Mauricio Alves Ribeiro andré Ibrahim David Luiz Arnaldo Szutan Gastroenterology Service Internal Medicine DepartmentSanta Casa School of Medical SciencesSao Paulo 01277-900Brazil Department of Surgery Liver and Portal Hypertension GroupSanta Casa School of Medical SciencesSao Paulo 01277-900Brazil Pathology Department Santa Casa School of Medical SciencesSao Paulo 01277-900Brazil GI Transplant Service Gastroenterology DepartmentUniversity of Sao PauloSao Paulo 01246-903Brazil

AIM:To investigate the association between nonalcoholic fatty liver disease(NAFLD) and liver cancer,and NAFLD prevalence in different liver ***:This is a retrospective study of the clinical,laboratory and histological data of 120 patients diagnosed with primary or secondary hepatic neoplasms and treated at a tertiary center where they underwent hepatic resection and/or liver transplantation,with subsequent evaluation of the explant or liver *** following criteria were used to exclude patients from the study:a history of alcohol abuse,hepatitis B or C infection,no tumor detected in the liver tissue examined by histological analysis,and the presence of chronic autoimmune hepatitis,hemochromatosis,Wilson's disease,or *** occurrence of NAFLD and the association with its known risk factors were *** risk factors considered were diabetes mellitus,impaired glucose tolerance,impaired fasting glucose,body mass index,dyslipidemia,and arterial *** of reticulin fibers in the hepatic neoplasms was assessed by histological analysis using slide-mounted specimens stained with either hematoxylin and eosin or Masson's trichrome and silver *** of tumor-free liver parenchyma was carried out to determine the association between NAFLD and its histological ***:No difference was found in the association of NAFLD with the general population(34.2% and 30.0% respectively,95%CI:25.8-43.4).Evaluation by cancer type showed that NAFLD was more prevalent in patients with liver metastasis of colorectal cancer than in patients with hepatocellular carcinoma and intrahepatic cholangiocarcinoma(OR = 3.99,95%CI:1.78-8.94,P < 0.001 vs OR = 0.60,95%CI:0.18-2.01,P = 0.406 and OR = 0.70,95%CI:0.18-2.80,P = 0.613,respectively).There was a higher prevalence of liver fibrosis in patients with hepatocellular carcinoma(OR = 3.50,95%CI:1.06-11.57,P = 0.032).Evaluation of the relationship between the presence of NAFLD,nonalcoholi

关键词： Hepatocellular carcinoma Colorectal liver metastases Intrahepatic cholangiocarcinoma Liver fibrosis Nonalcoholic fatty liver disease Nonalcoholic steatohepatitis

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An Analysis of the Spectral Energetics for a Planet Experiencing Rapid Greenhouse Gas Emissions

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Atmospheric and Climate Sciences 2017年第1期7卷 117-126页

作者： andré ferreira Aranha José Augusto P. Veiga UNINORTE Laureate International Universities Manaus Brazil Department of Meteorology Institute of Technology Amazonas State University Manaus Brazil

So far, energetics studies related to climate change have focused on the disturbed and undisturbed kinetic and potential energies, as well as their transformations, without dealing with the energetics involved in the phenomena of different spatial scales. Thus, the present work reports the first analysis of the spectral energetics for a condition of climate change, followed by the high-range emission scenario, RCP8.5, which originated from the new Max Planck Institute Earth System Model (MPI-ESM). The results showed that both types of generation (Go and Gn), baroclinic processes (Co and Cn), kinetic energies (Ko and Kn) and the barotropic process, Mn, significantly increase in the condition of a warming climate. Moreover, the results still reveal that in the most components of the energetics, is the planetary scale waves that are the most impacted under a climate change scenario. These results highlight that global warming can have different impacts on particular types of motions.

关键词： Energetics Global Energetics Spectral Energetics Climate Change MPI-ESM Model

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