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纳特竖蟾(无尾目:滑背蟾科)的细胞遗传、与相关属物种的核型相似性并兼论其分类地位(英文)

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动物学报 2007年第2期53卷 285-293页

作者： Fernando ANANIAS andré luis bombeiro Catia Donizete Brunaikovics da SILVA Ana Paula Zampieri SILVA Célio Fernando Baptista HADDAD Sanae KASAHARA Curso de Ciências Biológicas Universidade S■o Francisco (USF) Av. S■o Francisco de Assis 218-Jardim S■o José 12916-900 Bragana Paulista SP Brasil Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista (UNESP) Rio Claro SP Brasil Curso de Ciências Biológicas Universidade Braz Cubas (UBC) Mogi das CruzesSPBrasil Departamento de Zoologia Instituto de Biociências Universidade Estadual Paulista (UNESP) Rio Claro SP Brasil

本文报道纳特竖蟾(Eupemphix nattereri Steindachner,1863)巴西3个产地标本的核型,均为2n=22,由1对端部和10对中或亚中部着丝点染色体组成。仅一对NORs位于No.11染色体对长臂端部,而其位置有别于常见的具有标志性意义的近着丝点位置。... 详细信息

本文报道纳特竖蟾(Eupemphix nattereri Steindachner,1863)巴西3个产地标本的核型,均为2n=22,由1对端部和10对中或亚中部着丝点染色体组成。仅一对NORs位于No.11染色体对长臂端部,而其位置有别于常见的具有标志性意义的近着丝点位置。该NORs的位置为rDNA为探针的荧光原位杂交(FISH)所确认。各染色体对中,着丝点C-带明显,插入或端带偶见。某些标本的No.8同源染色体对间C-带的大小随异染色质化的程度不同而变化。居于较小的实验标本量,这种在3个产地的雌或雄性标本之一中观察到的C-带异型现象可能为种下细胞地理学变异,亦或为细胞学意义的性染体分化。3产地之一的标本核型的No.11着丝点C-带异染色质化的程度较高。CMA3染色检测到部分GC-rich区域,DAPI染色未显示任何AT-rich区。成功获得BrdU复制带,并将其与滑背蟾类动物(leiuperid)中近缘属及物种进行对比分析。比较结果表明,纳特竖蟾的核型与斑符泡蟾种组(Physalaemus signifergroup)难以相互区别,而与肿肋蟾属(Pleurodema Tschudi,1838)极为相似。核型数据支持形态学上将纳特竖蟾、二光肿肋蟾[Pleurodema diplolister(Peters,1870)]和短头肿肋蟾[***(Cope,1869)

关键词：纳特竖蟾染色体 Ag-NOR C-带荧光染色荧光原位杂交

Sexual dimorphism in the antitumor immune responses elicited by the combination of fasting and chemotherapy

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Cancer Communications 2024年第4期44卷 508-513页

作者： andrés Pastor-Fernández Manuel Montero Gómez de las Heras Jose Ignacio Escrig-Larena Marta Barradas Cristina Pantoja Adrian Plaza Jose luis Lopez-Aceituno Esther Durán Alejo Efeyan Maria Mittelbrunn Lola Martinez Pablo Jose Fernandez-Marcos Metabolic Syndrome Group(BIOPROMET) Madrid Institute for Advanced Studies(IMDEA)FoodE28049MadridSpain Molecular Biology Center Severo Ochoa(CBMSO) Spanish National Research Council(CSIC)Madrid Autonoma University(UAM)MadridSpain Department of Basic Medical Sciences Institute of Applied Molecular MedicineSchool of MedicineUniversity Studies Center(CEU)-San Pablo UniversityMadridSpain Metabolism and Cell Signaling Laboratory Spanish National Cancer Research Centre(CNIO) MadridSpain 5Flow Cytometry UnitBiotechnology ProgrammeSpanish National Cancer Research Centre(CNIO)MadridSpain

Fasting reduces chemotherapy toxicity[1],enhances immunogenic tumor cell death[2,3]and increases CD8^(+)T cell infiltration in tumors,particularly when combined with chemotherapy[2,3]or immunotherapy[4].More-over,fast... 详细信息

关键词： chemotherapy fasting immune

Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations

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World Journal of Gastroenterology 2017年第5期23卷 800-809页

作者： andrés Jenuer Matta Alvaro Jairo Pazos Javier andrés Bustamante-Rengifo luis Eduardo Bravo Registro Poblacional de Cáncer de Cali Department of PathologySchool of MedicineUniversidad del Valle Department of Biology Universidad de Narino

AIMTo compare the genomic variability and the multiple colonization of Helicobacter pylori (H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer (GC): Túquerres-Nariño (High risk) and Tumaco-Nariño (Low risk).METHODSFour hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa, (31/206) of the high risk population of GC (Túquerres) and (41/203) of the low risk population of GC (Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence *** proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversity among isolates of Túquerres (0.13) than those of Tumaco (0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco (P = 0.05).CONCLUSIONIn Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC.

关键词： Helicobacter pylori Pathogenicity islet cag cagA vacA Multiple colonization PCR-RAPD

The flowerpiercers interactions with a community of high Andean plants

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Avian Research 2021年第2期12卷 181-194页

作者： Jairo andrés Cuta-Pineda luis Alejandro Arias-Sosa Roxibell C.Pelayo Research Group“Grupo Ecología de Organismos(GEO-UPTC)” TunjaColom-bia Maestría en Ciencia Biológicas Universidad Pedagógica y Tecnológica de ColombiaTunjaColombia Instituto de Ciencias Ambientales y Ecológicas(ICAE) Universidad de Los AndesMéridaVenezuela

Background:Flowerpiercers(Diglossa)are traditionally considered as“parasites”of the pollination processes,as they can access the nectar without entering in contact with the reproductive structures of the ***,the effect of flowerpiercers seems to vary according to their behavior and the flower’s ***,in this work,we aimed to explore the floral characteristics that may determine the susceptibility to robbing and pollen transport by ***,we identified the potential types of interactions and studied interaction network ***:We collected the information of 16 ornithophilic plants regarding their floral traits and robbing ***,we captured 4 species of flowerpiercers and evaluated pollen transport(frequency and loads).We tested the correlation between floral traits,robbing frequency,and pollen ***,we used these variables in a cluster and principal component analyses to identify the potential types of ***,we analyzed and com-pared the structure of the plants-flowerpiercers interaction ***:Nectar production significantly influenced both nectar robbing and pollen *** the corolla length was only correlated to the robbing ***,we found that particular flowerpiercers species trans-ported higher loads of some plant pollen,which can be related to the differences in behavior and morphometric *** proposed the classification of five different types of plant-flowerpiercer interactions,that showed differ-ent potential mutualist or antagonist relations based on the affectation of nectar robbing and the service of pollen *** interaction networks consisted of 49 links,with 2.4 links per species,and presented indicators of a medium to high resilience,stability,and resistance(nestedness,connectance,and robustness).Also,the network presented medium to low specialization and substantial niche ***:The ecological role of

关键词： Diglossa Flower traits Nectar robbing Nectivorous birds Pollen transport Pollination

Application of data science in the prediction of solar energy for the Amazon basin:a study case

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Clean Energy 2023年第6期7卷 1344-1355页

作者： andréluis Ferreira Marques Márcio JoséTeixeira Felipe Valencia de Almeida Pedro Luiz Pizzigatti Corrêa Polytechnic School of the University of Sao Paulo Av.Prof.Luciano Gualberto380-ButantaSao Paulo-SP05508-010Brazil Institute of Physics of the University of Sao Paulo Brazil

The need for renewable energy sources has challenged most countries to comply with environmental protection actions and to handle climate *** energy figures as a natural option,despite its *** has a green energy matrix with significant expansion of solar form in recent *** preserve the Amazon basin,the use of solar energy can help communities and cities improve their living standards without new hydroelectric units or even to burn biomass,avoiding harsh environmental *** novelty of this work is using data science with machine-learning tools to predict the solar incidence(W.h/m^(2))in four cities in Amazonas state(north-west Brazil),using data from NASA satellites within the period of ***-tree-based models and vector autoregressive(time-series)models were used with three time aggregations:day,week and *** predictor model can aid in the economic assessment of solar energy in the Amazon basin and the use of satellite data was encouraged by the lack of data from ground *** mean absolute error was selected as the output indicator,with the lowest values obtained close to 0.20,from the adaptive boosting and light gradient boosting algorithms,in the same order of magnitude of similar references.

关键词： solar energy renewable energy Amazon basin machine learning time series data science decision-trees ensemble vector autoregression

Development of Junction Elements from Study of the Bionics

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Journal of Bionic Engineering 2007年第1期4卷 41-46页

作者： Wilson Kindlein Junior luis Henrique Alves Cndido andré Canal Marques Sandra Souza dos Santos Maurício da Silva Viegas Laboratory of Design and Material Selection-LdSM-Materials Department (DEMAT) School of Engineering–EE Federal University of Rio Grande do Sul-Brazil-UFRGS Brazil

The applications of bionic methodology developed by the Laboratory of Design and Material Selection as basis in the creation of junction elements were demonstrated. These elements favor the application of Ecodesign in reference to the effectiveness of product dismount aiming the reduction of ambient impact in all its phases of use. The creation, the development and the confection of new junction elements were described, and case studies of new products developed specifically with this purpose were presented.

关键词： bionics Ecodesign junction elements

Preparation and Characterization of Flexible, Transparent and Thermally Stable Aromatic Co-polyamides

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Chinese Journal of Polymer Science 2019年第2期37卷 136-141页

作者： José Antonio Tec-Sánchez andrés Iván Oliva Arias Manuel Aguilar-Vega Juan Valerio Cauich-Rodríguez José luis Santiago-García Unidad de Materiales Centro de Investigación Científica de Yucatán A. C. Centro de Investigación y de Estudios Avanzados del IPN Unidad Mérida Departamento de Física Aplicada

Two aromatic co-polyamides were synthesized combining two diacid monomers containing bulky pendant groups, 5-(9,10-dihydro-9,10-ethanoanthracene-11,12-dicarboximido)isophthalic acid(DEAIA) and 5-tert-butylisophthalic acid(TERT), with 4,4′-(hexafluoroisopropylidene)dianiline(HFA) or 2,3,5,6-tetramethyl-1,4-phenylenediamine(Durene) by direct polycondensation. The structures of the obtained aromatic co-polyamides were confirmed by FTIR, Raman and 1 H-NMR. The co-copolyamide films, DHTH and DDTD, exhibited rms-roughness values between 0.94 and 1.60 nm, respectively. Moreover, they presented good thermal stability up to300 °C. Young's moduli of the co-polyamide films were between 4.1 and 4.3 GPa. X-ray diffraction results showed that the co-polyamide films were amorphous due to the incorporation of both bulky pendant groups, tert-butyl and dibenzobarrelene. The combination of bulky pendant groups provided intrinsically transparent co-polyamide films with a transmittance higher than 88% in the range of 400-780 *** to these outstanding film and optical properties, they are suggested to be flexible substrates in applications for solar cell and other portable electronic devices.

关键词： Co-polyamides Flexible films Transmittance Thermal stability Polycondensation

Fractions of a chloroform extract of ajenjo leaves (Artemisiamendozana DC. var. mendozana) inhibit the proliferation,viability and clonogenicity of B16-F0 melanoma cells

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BIOCELL 2020年第3期44卷 293-299页

作者： MARÍA ELISA MILLÁN MARÍA FERNANDA MARRA LEONARDO andrÉS SALVARREDI EMILIO FERNANDO LIZARRAGA luis ALBERTO LOPEZ Universidad Nacional de Cuyo MendozaArgentina Comisión Nacional de Energía Atómica Buenos AiresArgentina Fundación Escuela de Medicina Nuclear MendozaArgentina Fundación Miguel Lillo y Universidad Nacional de Tucumán San Miguel de TucumánArgentina

The ajenjo,Artemisia mendozana ***(Asteraceae),grows in the Andean foothills of Mendoza and San Juan,Argentina,and is used as a medicinal plant for its antispasmodic and antifungal *** aim of this work was to obtain fractions of a chloroform extract of ajenjo leaves and to evaluate the in vitro effects on proliferation,viability and clonogenicity of B16-F0 melanoma *** a silica gel chromatography column,120 fractions were collected and grouped according to the chromatographic profile in 9 main fractions(F1–F9).Their major compounds identified were:terpenes(F1),terpenes and sesquiterpene lactones(F2–F3),sesquiterpenes(F4–F6)and phenols and sesquiterpenes(F7-9).B16-F0 cells were incubated for 72 h with DMSO(vehicle)or 0.1 mg/ml F1–*** 72 h of culture,F1 decreased both the growing index(GI)and cell viability.F2 and F3 both decreased GI and only F3 decreased clonogenic activity.F4 and F5 both decreased *** F5 decreased cell viability and F4 decreased ***,fractions F6–F8 did not affect any of the cell parameters assayed,while F9 decreased cell viability and inhibited clonogenicity.

关键词： Terpenes Sesquiterpene lactones Sesquiterpenes Phenols

Chilaiditi syndrome in pediatric patients - Symptomatic hepatodiaphragmatic interposition of colon: A case report and review of literature

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World Journal of Clinical Pediatrics 2021年第3期10卷 40-47页

作者： luis Caicedo Paul Wasuwanich andrés Rivera Maria S Lopez Wikrom Karnsakul Division of Pediatric Gastroenterology Hepatologyand NutritionNicklaus Children’s HospitalMiamiFL 33155United States Department of Medicine University of Florida College of MedicineGainesvilleFL 32610United States Department of Pediatrics Icahn School of Medicine at Mount SinaiNew YorkNY 10092United States Department of Pediatrics Nicklaus Children’s HospitalMiamiFL 33155United States Division of Pediatric Gastroenterology Hepatologyand NutritionDepartment of PediatricsJohns Hopkins University School of MedicineBaltimoreMD 21287United States

BACKGROUND Chilaiditi syndrome is a rare disorder characterized by the hepatodiaphragmatic interposition of the *** SUMMARY Here we report a case of a 12-year-old male who was admitted to the pediatric intensive care unit secondary to abdominal pain and severe respiratory *** was treated conservatively but the symptoms persisted requiring a surgical *** there have been several cases of Chilaiditi syndrome reported in adults,there is a scarcity of cases reported in the pediatric *** review of the literature found only 30 pediatric cases,including our reported case,with Chilaiditi syndrome,19(63%)of which were *** median age of diagnosis was 4.5 years old with an interquartile range of 2.0-10.0 *** our review,we found that the most common predisposing factors in children are aerophagia(12/30 cases)and constipation(13/30 cases).Ninety percent of the cases presented with complete intestinal interposition,in 100%of which,the colon was *** of the 30 cases were associated with *** In the pediatric population,conservative(21/30 cases)and surgical(8/30 cases)treatment approaches have produced satisfactory outcomes for all the patients,regardless of approach.

关键词： Abdominal pain Dyspnea Constipation Rare diseases Respiratory insufficiency Colon Case report