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Global mapping of volumetric water retention at 100,330 and 15000 cm suction using the WoSIS database

International Soil and Water Conservation Research 2023年第2期11卷 225-239页

作者： Maria Eliza Turek Laura Poggio Niels H.Batjes Robson andre Armindo Quirijn de Jong van Lier Luis de Sousa Gerard B.M.Heuvelink ISRIC-World Soll Information Wageningemthe Netherlands Graduate Program in Environmental Engineering Federal University of ParunaCurtibaR Brazil Soll Geography and Landscope Group Wageningen University Wageningenthe Netherlands Department of Physics Federul University of LavrasMGBrarll CENA-Universiy of Sao Poulo PinacicabasaoBrazil

Present global maps of soil water retention(SWR)are mostly derived from pedotransfer functions(PTFs)applied to maps of other basic soil *** an alternative,'point-based'mapping of soil water content can improve global soil data availability and *** developed point-based global maps with estimated uncertainty of the volumetric SWR at 100,330 and 15000 cm suction using measured SWR data extracted from the WoSIS Soil Profile Database together with data estimated by a random forest PTF(PTF-RF).The point data was combined with around 200 environmental covariates describing vegetation,terrain morphology,climate,geology,and hydrology using *** total,we used 7292,33192 and 42016 SWR point observations at 100,330 and 15000 cm,respectively,and complemented the dataset with 436108 estimated values at each *** cross-validation yielded a root Mean Square Error(RMSE)of6380,7.112 and 6.48510^(-2)cm^(3)cm^(-3),and a Model Efficiency Coefficient(MEC)of0.430,0386,and 0.471,respectively,for 100,330 and 15000 *** results were also compared to three published global maps of SWR to evaluate differences between point-based and map-based mapping ***-based mapping performed better than the three map-based mapping approaches for 330 and 15000 cm,while for 100 cm results were similar,possibly due to the limited number of SWR observa-tions for 100 *** sources or uncertainty identified included the geographical clustering of the data and the limitation of the covariates to represent the naturally high variation of SWR.

关键词： Digital soil mapping Soil hydraulic properties Pedometrics SoilGrids

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Hemoconcentration is a poor predictor of severity in acute pancreatitis

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World Journal of Gastroenterology 2005年第44期11卷 7018-7023页

作者： José M.Remes-Troche andrés Duarte-Rojo Gustavo Morales Guillermo Robles-Díaz Department of Gastroenterology

AIM: To determine whether the hematocrit (Hct) at admission or at 24 h after admission was associated with severe acute pancreatitis (ap), organ failure (OF), and pancreatic ***: A total of 336 consecutive patients with a first ap episode were studied. Etiology, Hct values at admission and at 24 h, development of severe ap according to Atlanta's criteria, pancreatic necrosis, OF and mortality were recorded. Hemoconcentration was defined as Hct level ＞44% for males and ＞40% for females. The t-test and χ2 test were used to assess the association of hemoconcentration to the severity, necrosis and *** accuracy was also ***: Biliary disease was the most frequent etiology(n = 148). Mean Hct levels at admission were 41±6%for females and 46±7% for males (P＜0.01). Seventyeight (23%) patients had severe ap, and OF developed in 45 (13%) patients. According to contrast-enhanced computed tomography scan, 36% (54/150) patients showed pancreatic necrosis. Hct levels were elevated in58% (55/96) and 61% (33/54) patients with interstitial and necrotizing pancreatitis, respectively. Neither Hct levels at admission nor hemoconcentration at 24 h were associated with the severity, necrosis or OF. Sensitivity,specificity and positive predictive values for both determinations were very low; and negative predictive values were between 61% and 86%, being the highest value for ***: Hct is not a useful marker to predict a worse outcome in acute pancreatitis. In spite of the high negative predictive value of hemoconcentration, the prognosis gain is limited due to an already high incidence of mild disease.

关键词：血液浓缩急性胰腺炎治疗并发症

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Effectiveness of different biochars in remediating a salt-affected Luvisol in Northeast Brazil

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Biochar 2021年第2期3卷 149-159页

作者： Wallace Melo dos Santos Maria Isidoria Silva Gonzaga JoséAiron da Silva andréQuintão de Almeida JoséCarlos de Jesus Santos Thomas Antonio Silva Gonzaga Idamar da Silva Lima EloáMoura Araújo Water Resources Graduate Program Federal University of SergipeSão CristóvãoSE 49100‑000Brazil Agronomy Department Federal University of SergipeSão CristóvãoSE 49100‑000Brazil Agriculture Engineering Department Federal University of SergipeSão CristóvãoSE 49100‑000Brazil University of Notre Dame Notre DameIN 46556USA Soils and Agricultural Engineering Department Federal University of ParanáCuritibaPR 80060‑000Brazil

Biochar has been proposed as a soil amendment to reclaim salt-affected ***,the evaluation of the different biochar types in these soils is essential due to the significant variation among biochar *** this study,biochars from sugarcane bagasse(SB),orange bagasse(OB),and corncob(CB)were applied alone and in the presence of gypsum(G)to test their potential to remediate a highly degraded saline-sodic soil in Brazil´s Northeast *** that,we conducted a laboratory column-leaching experiment and a greenhouse *** the column-leaching test,we analyzed the sodium adsorption ratio(SAR),the electrical conductivity of the saturation extract(ECe),the concentrations of major ions,and the exchangeable sodium percentage(ESP).Maize plants were grown in the leached soil in a ***-and corncob-derived biochars were more efficient than gypsum in reducing the soil salinity,bringing the indicators below threshold values,and optimizing soil reclamation *** ECe,SAR,and ESP were reduced to 3.42 dS m−1,1.64(mmolc dm−3)0.5 and 4.86%,respectively,in the SB treatment;and to 3.19 dS m−1,0.88(mmolc dm−3)0.5 and 2.53%,respectively,in the CB *** bagasse biochar did not effectively reduce the salinity *** biochar treatments improved seed germination and plant *** SB,CB,SBG,and CBG treatments increased plant height(478–558%),shoot mass(783–983%),and root mass(386–500%),***,the application of SB and CB is an effective alternative to remediate saline-sodic soils and to reduce the impact of soil salinity in the environment.

关键词： Biochar Soil remediation Soil salinity Corn growth

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Validation of Reference Genes for mRNA Quantification in Adjuvant Arthritis

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Open Journal of Rheumatology and Autoimmune Diseases 2012年第3期2卷 64-72页

作者： Muhammad Ayaz Alam Qureshi Aisha Siddiqah Ahmed Jian Li andré Stark Per Eriksson Mahmood Ahmed Department of Clinical Neurosciences Karolinska Institutet Stockholm Sweden Department of Clinical Sciences Danderyd Hospital Karolinska Institutet Stockholm Sweden Department of Medicine Karolinska University Hospital Karolinska Institutet Stockholm Sweden Department of Molecular Medicine and Surgery Karolinska University Hospital Karolinska Institutet Stockholm Sweden Department of Neurobiology Care Sciences and Society Karolinska Institutet Stockholm Sweden

Real time quantitative PCR (RT-qPCR) requires a method to normalize the expression of target genes against an en- dogenous reference gene. It is known that commonly used housekeeping genes (HKGs) vary tremendously in inflam- matory conditions;however information about the stability and expression of HKGs in chronic inflammatory joint dis- ease such as rheumatoid arthritis (RA) is scarce. The expressional stability of 10 commonly used HKGs was analyzed in the neuronal (spinal cord, dorsal root ganglia) and in the musculoskeletal tissues (tendon, muscle, epiphysis, capsule, periosteum and ankle joint) using RT-qPCR in the rat model of RA. In individual tissues, suitable HKGs were selected by | △Ct| (│Ct control-Ct arthritis│) and further analyzed by using software programs;geNorm and normfinder. We found hypoxanthine-guanine phosphoribosyl tranferase (HPRT) as the most stable gene except ankle joint while glyce-raldehyde-3-phosphate dehydrogenase (GapDH) was found as the least stable gene in musculoskeletal tissues. In in-flamed ankle joint where no reference gene was found to be stably expressed, an inflammatory cell marker CD3 was used to normalize peptidylprolyl isomerase B (PPIB), the most homogenous HKG identified among the 10 HKGs. The normalized PPIB was then used to analyze the gene expression of neurokinin 1 (NK1), receptor of substance P, a potent pro-inflammatory mediator. We observed a 3.5 fold increase (p = 0.009) in NK1 expression in inflamed ankle joint compared to control. Our results indicate that reference genes stability should be evaluated before using them as refer- ence during inflammatory conditions. In tissues with intense inflammatory cell infiltration, an inflammatory cell marker should be used to normalize the selected reference gene to avoid erroneous results.

关键词： Quantitative PCR Adjuvant Arthritis Housekeeping Gene

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Chromosome Elimination in Intergeneric Hybrid of <i>Oryza sativa</i>×<i>Luziola peruviana</i>

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Agricultural Sciences 2014年第13期5卷 1344-1350页

作者： Pablo Emilio Moreno Creucí María Caetano Cristian andrés Olaya Tomas Cipriano Agrono Edgar Alfonso Torres Universidad Nacional de Colombia Palmira Colombia Centro Internacional de Agricultura Tropical CIAT Palmira Colombia

Oryza sativa and Luziola peruviana present a diploid chromosome number of 2n = 24 and basic number x = 12, confirmed by means metaphase chromosomes counts in young root tips of these species. Hybrid plants O. sativa × L. peruviana, with 2n = 24 chromosomes are originated from simple crosses and present abnormalities in the meiotic behavior, chromosomal aberrations and cytological alteration. This genetic incompatibility is caused by different factors as absence of pairing and recombination, different spindles arrangements, cytoskeleton instability, apoptosis process and chromosomal elimination, leading to micronuclei formation, unbalanced gametes and sterile pollen grains. The chromosome elimination is established as a dynamic process of stabilization of the genome that occurs during hybridization. It is a common phenomenon among intergeneric crosses and corresponds to cytoplasmic and nuclear bodies that reflect chromosomal aberrations resulting from the combination of two genomes with high genetic distance. The genomic conflict occurs in meiosis, possibly by asynchronism and cell cycle length of the genomes involved, or by time differences in replication between parental species leading to strand breaks and genomic rearrangements.

关键词： Rice Luziola Unbalanced Gametes Meiosis

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Aging Effects on Neuromuscular Activity in Karate Practitioners

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Journal of Sports Science 2015年第5期3卷 203-213页

作者： AntOnio Manue VitOria Vences de Brito Mario andr Ferreira Rodrigues Wojciech Jan Cynarski Carlos Gutiorrez Garcia Sport Sciences School of Rio Maior Polytechnic Institute of Santar~m 2040-413 Rio Maior Portugal Life Quality Research Centre-CIEQV Complexo Andaluz Apartado 279 2001-904 SantarOm Portugal International Martial Arts and Combat Sports Scientific Society--IMACSSS 35-959 Rzeszow A. Towarnickiego Str. 3/17APoland Faculty of Physical Education University of RzeszEw Rzeszow 35-959 Poland Department of Physical and Sport Education Faculty of Physical and Sport Sciences University of Le6n Vegazana Campus24071-Le6n Spain

Aging leads to a general decline in body functions that affects quality of life. Sports practice is recommended as a means to attenuate the impact of the aging process. The aim of this study was to identify and analyze the neuromuscular activity and its patterns on active karate practitioners and assess the effects of the aging process. Two groups of black belt male karate practitioners, one with nine practitioners over 50 years old and the other with 21 practitioners aged between 20-30 years old performed the frontal kick mae-geri. Kinematic and electromyographic data of five muscles were collected from the right leg. Results showed two distinct periods of muscular activation in the mae-geri performance, with similar muscles activity patterns between groups. Nevertheless, some differences were found in the muscles" activity onset and offset, in root mean square and in inter-muscle delay and co-contraction periods. Those differences indicated that aging affects the neuromuscular activity in the mae-geri performance of the oldest karate practitioners. Nevertheless, the results showed that lifelong practice of karate can preserve the quality of the neuromuscular activity in older practitioners when they perform a kick, and this could be associated with benefits to balance control.

关键词：运动科学体育运动训练体育训练

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Modeling the Hydrological Dynamic of the Breeding Water Bodies in Barkedji’s Zone

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Journal of Water Resource and Protection 2014年第8期6卷 741-755页

作者： Mamadou Bop Angelina Amadou Ousmane Seidou Cheikh Mouhamed Fadel Kébé Jacques andré Ndione Soussou Sambou Ibrah Seidou Sanda Agrhymet Regional Center Niamey Niger Centre de Suivi écologique (CSE) Dakar Sénégal Centre International de Formation et de Recherche en Energie Solaire (CIFRES) Département de Génie Civil Ecole Supérieure Polytechnique Université Cheikh Anta Diop Dakar Sénégal Department of Civil Engineering University of Ottawa Ottawa Canada Laboratoire d’Hydraulique et de Mécanique des Fluides Département de Physique Faculté des Sciences et Techniques Université Cheikh Anta Diop de Dakar Dakar Sénégal

Temporary water bodies’ dynamics play an important role in the epidemiological chain-borne diseases such as Rift Valley fever as they are the main breeding habitats for mosquitoes. During the rainy season, hundreds of these temporary water bodies appear and grow in the Ferlo region (Senegal). The purpose of this research is to generate historical and future time series water levels and areas at three temporary ponds located in the environment and health observatory of Barkedji. A simple lumped hydrological model was developed for that purpose. It describes each pond watershed as three interconnected reservoirs: canopy, surface storage and soil storage and uses a linear relation to describe infiltration, percolation and baseflow (out of the soil reservoir). Given the depth of the water table in the region, percolation out of the soil surface is considered lost. Evapotraspiration was calculated using the Penman equation and withdraws water from the canopy and surface water reservoirs. Excess runoff from the soil storage is turned into runoff using a triangular unit hydrograph. The calibration was done using two years of hydrological and climatic data collected during the 2011 and 2012 rainy seasons. The calibration was successful and water level in the two ponds was simulated with a root Mean Square Error (RMSE) of 11.2 to 15 cm. Because of the short duration of the observation, no validation could be done. Given the excellent agreement of the simulated and observed water levels during the calibration phase, the modeling exercise was considered to be successful. The developed models were used to generate historical time series of pond areas and correlate these to mosquitoes’ infestation in the region. Future time series of pond areas were also generated using downscaled outputs of three regional climate models from the AMMA ENSEMBLES experiment. The generated pond levels and areas are being used to assess the evolution of the disease in the next 40 years.

关键词： Breeding Areas Hydrology Pond Dynamics Climate Change

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A DL-4- and TNFα-based culture system to generate high numbers of nonmodified or genetically modified immunotherapeutic human T-lymphoid progenitors

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Cellular & Molecular Immunology 2021年第7期18卷 1662-1676页

作者： Ranjita Devi Moirangthem Kuiying Ma Sabrina Lizot Anne Cordesse Juliette Olivré Corinne de Chappedelaine Akshay Joshi Agata Cieslak John Tchen Nicolas Cagnard Vahid Asnafi Antonio Rausell Laura Simons Julien Zuber Tom Taghon Frank J.T.Staal Françoise Pflumio Emmanuelle Six Marina Cavazzana Chantal Lagresle-Peyrou Tayebeh Soheili Isabelle andré Universitéde Paris Imagine InstituteLaboratory of Human LymphohematopoiesisINSERM UMR 1163ParisFrance Laboratory of Onco-Hematology AP-HPHôpital Necker-Enfants Malades.ParisFrance Universitéde Paris Institut Necker-Enfants Malades(INEM)INSERM UMR 1151ParisFrance Plateforme Bio-informatique UniversitéParis DescartesStructure Fédérative de Recherche NeckerINSERM US24/CNRS UMS 3633ParisFrance Universitéde Paris Imagine InstituteLaboratory of Clinical BioinformaticsINSERM UMR 1163ParisFrance Department of Biotherapy Clinical Investigation Center AP-HPHôpital Necker-Enfants MaladesParisFrance Department of Adult Kidney Transplantation AP-HPHôpital NeckerParisFrance Cancer Research Institute Ghent(CRIG) GhentBelgium Department of Diagnostic Sciences Faculty of Medicine and Health SciencesGhent UniversityGhentBelgium Department of Immunohematology&Blood Transfusion Leiden University Medical CenterLeidenthe Netherlands Team Niche and Cancer in Hematopoiesis Universitéde Paris and UniversitéParis-SaclayINSERMiRCM/IBFJ CEAUMR StabilitéGénétique Cellules Souches et RadiationsFontenay-aux-RosesFrance

Several obstacles to the production,expansion and genetic modification of immunotherapeutic T cells in vitro have restricted the widespread use of T-cell *** the context of HSCT,delayed naïve T-cell recovery contributes to poor outcomes.A novel approach to overcome the major limitations of both T-cell immunotherapy and HSCT would be to transplant human T-lymphoid progenitors(HTLPs),allowing reconstitution of a fully functional naïve T-cell pool in the patient ***,it is challenging to produce HTLPs in the high numbers required to meet clinical ***,we found that adding tumor necrosis factor alpha(TNFα)to a DL-4-based culture system led to the generation of a large number of nonmodified or genetically modified HTLPs possessing highly efficient in vitro and in vivo T-cell potential from either CB HSPCs or mPB HSPCs through accelerated T-cell differentiation and enhanced HTLP cell cycling and *** study provides a clinically suitable cell culture platform to generate high numbers of clinically potent nonmodified or genetically modified HTLPs for accelerating immune recovery after HSCT and for T-cell-based immunotherapy(including CAR T-cell therapy).

关键词： Human T-lymphoid progenitor Tumor necrosis factor alpha Delta-like ligand 4 Hematopoietic stem and progenitor cells Mobilized peripheral blood

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Medicinal properties of Angelica archangelica root extract: Cytotoxicity in breast cancer cells and its protective effects against in vivo tumor development

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Journal of Integrative Medicine 2019年第2期17卷 132-140页

作者： Carlos R.Oliveira Daniel G.Spindola Daniel M.Garcia Adolfo Erustes Alexandre Bechara Caroline Palmeira-dos-Santos Soraya S.Smaili Gustavo J.S.Pereira andré Hinsberger Ezequiel P.Viriato Maria Cristina Marcucci Alexandra C.H.F.Sawaya Samantha L.Tomaz Elaine G.Rodrigues Claudia Bincoletto Departamento de Farmacologia Escola Paulista de Medicina (EPM) Universidade Federal de Sao Paulo (UNIFESP) Grupo de Fitocomplexos e Sinalizacao Celular Escola de Ciências da Saúde Universidade Anhembi Morumbi Faculdade de Ciências Farmacêuticas e Bioquímicas Oswaldo Cruz Laboratório de Produtos Naturais e Quimiometria Programa de pós-graduacao em Farmácia e Biotecnologia Universidade Anhanguera de Sao Paulo Faculdade de Ciências Farmacêuticas Universidade Estadual de Campinas (UNICAMP) Unidade de Oncologia Experimental EPM Universidade Federal de Sao Paulo (UNIFESP)

Objective: Although Angelica archangelica is a medicinal and aromatic plant with a long history of use for both medicinal and food purposes, there are no studies regarding the antineoplastic activity of its *** study aimed to evaluate the cytotoxicity and antitumor effects of the crude extract of A. archangelica root(CEAA) on breast ***: The cytotoxicity of CEAA against breast adenocarcinoma cells(4T1 and MCF-7) was evaluated by a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay. Morphological and biochemical changes were detected by Hoechst 33342/propidium iodide(PI) and annexin V/PI staining. Cytosolic calcium mobilization was evaluated in cells staining with FURA-4 NW. Immunoblotting was used to determine the effect of CEAA on anti-and pro-apoptotic proteins(Bcl-2 and Bax, respectively).The 4 T1 cell-challenged mice were used for in vivo ***: Using ultra-high-performance liquid chromatography–mass spectrometry analysis, angelicin, a constituent of the roots and leaves of A. archangelica, was found to be the major constituent of the CEAA evaluated in this study(73 mg/mL). The CEAA was cytotoxic for both breast cancer cell lines studied but not for human fibroblasts. Treatment of 4 T1 cells with the CEAA increased Bax protein levels accompanied by decreased Bcl-2 expression, in the presence of cleaved caspase-3 and cytosolic calcium mobilization, suggesting mitochondrial involvement in breast cancer cell death induced by the CEAA in this cell line. No changes on the Bcl-2/Bax ratio were observed in CEAA-treated MCF7 cells. Gavage administration of the CEAA(500 mg/kg) to 4T1 cell-challenged mice significantly decreased tumor growth when compared with untreated ***: Altogether, our data show the antitumor potential of the CEAA against breast cancer cells in vitro and in vivo. Further research is necessary to better elucidate the pharmacological application of the CEAA in breast cancer therapy.

关键词： Angelica archangelica Umbelliferae apoptosis Cell death Breast cancer Angelicin

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Physiological Performance of Common Bean Seeds Treated with Bioactivator with and without Moisture Restriction

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American Journal of Plant Sciences 2014年第26期5卷 3769-3776页

作者： andréia da Silva Almeida Francisco Amaral Villela Villela Geri Eduardo Meneghello Cristiane Deuner Lilian Madruga de Tunes Paulo Dejalma Zimmer Adilson Jauer PPG em Ciência e Tecnologia de Sementes Universidade Federal de Pelotas Pelotas Brasil Syngenta Crop Protection Pelotas Brasil

The commonbean (Phaseolus vulgaris L.) is an important agricultural crop due to its nutritional properties. Insecticides and fungicides employed to control pests and diseases in the common bean may cause as yet unknown effects on the plant’s morphology and metabolism. Bioactivators are complex organic substances, capable of exerting modification upon growth patterns, plant DNA transcription, gene expression, metabolic enzymes and mineral nutrition. Thiamethoxam insecticide has shown positive action on the enhancement of vigor expression, phytomass accretion and the promotion of deeper root systems. The aim of this work was to evaluate the effect of thiamethoxam on the physiological performance of common bean seeds. Seeds from cultivars Pérola and IapAR Siriri were treated to thiamethoxam concentrations of 0, 100, 200, 300, 400 and 500 mL·kg-1 seed. Physiological quality was measured through tests for germination value, first count, cold test, accelerated aging and seedling shoot and root lengths, all undertaken in the presence and absence of moisture stress. Thiamethoxam stimulated the physiological performance of common bean seeds under moisture stress or in the absence of it, with levels varying according to cultivar and seed lot. The best physiological seed performance was obtained within the range of 200 mL·kg-1 seed to 400 mL·kg-1 seed.

关键词： Phaseolus vulgaris L. Bioactivator Seeds Vigor

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