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Evaluation of a new qPCR test to identify the organisms causing high total bacterial count in bulk tank milk

Journal of Integrative Agriculture 2018年第6期17卷 1241-1245页

作者： Jorgen Katholm Lene Trier Olesen anders petersen Snorri Sigurdsson DNA Diagnostic A/S Risskov 8240Denmark ARLA Foods amba Viby 8260Denmark SEGES Aarhus N 8200Denmark

Milk quality in bulk tank milk （BTM） is measured by flow cytometry technology as total bacterial count （TBC） and somatic cell count （SCC）. To investigate SCC problems, culture or PCR can be used to identify mastitis causing bacteria, e.g., Mastit 4, a commercially available qPCR test. TBC in BTM can be investigated further using culture-based methods such as standard plate count, laboratory pasteurization count, coliform count, and spore counts. To our knowledge, no qPCR addressing the bacteria involved in TBC has been commercially introduced. The aim of this study is to evaluate a recently introduced 3-h qPCR test, TBC 4. The TBC 4 qPCR detects four target groups, Pseudomonas, Streptococci, Enterobacteriacea/ Enterococcus, and Bacillus/Clostridia. These target groups relate to problems on the farm such as cooling, mastitis, environment, and silage. We will continue with new research to compare the TBC 4 qPCR test with traditional culture. For this study, BTM samples from different TBC intervals were selected based on BactoCount results found at routine payment investigation at Eurofins laboratory （Vejen, Denmark）. These samples were analyzed using TBC 4 qPCR assay within 24 h. In total, 346 BTM samples were divided into six different intervals of colony forming units （CFU）. For all four targets in each of the different intervals of CFU, the percent of positive samples, the average Ct-value, the percent of positive samples with Ct〈30 and Ct〈25 were calculated. For Pseudomonas, Streptococci, and Enterobacteriacea/Enterococcus, the number of positive samples with lower Ct-values （high bacteria content） correlated with the CFU mL-1. We found Enterobacteriacea/ Enterococcus, Pseudomonas, and Streptococci in high number of bacteria （Ct〈25） in 25, 19 and 56％ of samples with CFU mL-1 between 50001-100000 and 53, 44, and 39％ in samples with CFU mL-1〉100000. The TBC 4 qPCR test showed to be a strong and fast tool for farmers, advisors and service technicians to address probl

关键词： TBC bulk tank milk qPCR milk quality

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Can fecal microbiota transplantation cure irritable bowel syndrome?

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World Journal of Gastroenterology 2017年第22期23卷 4112-4120页

作者： Sofie Ingdam Halkjar anders Watt Boolsen Stig Günther Alice Hojer Christensen Andreas Munk petersen Department of Gastroenterology Copenhagen University Hospital Hvidovre Department of Gastroenterology Aleris-Hamlet Hospitals Copenhagen Department of Clinical Microbiology Copenhagen University Hospital Hvidovre Faculty of Health and Medical Sciences University of Copenhagen

To verify the utility of treatment with fecal microbiota transplantation (FMT) in patients with irritable bowel syndrome (IBS).METHODSWe searched EMBASE, Cochrane Library and PubMed in March, 2017. The reviewed literature was based on two systematic searches in each of the databases. The MeSH terms used were IBS and fecal microbiota transplantation and the abbreviations IBS and FMT. Reference lists from the articles were reviewed to identify additional pertinent *** total of six conference abstracts, one case report, one letter to the editor, and one clinical review were included. In the final analysis, treatment of 48 patients was evaluated. Treatment revealed an improvement in 58% of cases. The varying structure of the nine included studies must be taken into *** on FMT and IBS are too limited to draw sufficient conclusions. Standardized double blinded randomized clinical trials need to be carried out to evaluate the effect of FMT on IBS.

关键词： Fecal microbiota transplantation Microbiota Irritable bowel syndrome

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Haemorrhagic septicaemia type B associated outbreak of fowl cholera in turkeys

Haemorrhagic septicaemia type B associated outbreak of fowl ...

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The 15th World Veterinary Poultry Congress

作者： Magne Bisgaard Andreas petersen anders M Bojesen Jens P Christensen Henrik Christensen Department of Veterinary Pathology Faculty of Life SciencesUniversity of CopenhagenDenmark Department of Veterinary Pathology Faculty of Life SciencesUniversity of CopenhagenDenmark Department of Veterinary Pathology Faculty of Life SciencesUniversity of CopenhagenDenmark Department of Veterinary Pathology Faculty of Life SciencesUniversity of CopenhagenDenmark Department of Veterinary Pathology Faculty of Life SciencesUniversity of CopenhagenDenmark

＜正＞Haemorrhagic septicaemia is an acute disease principally affecting cattle and water buffaloes character- ized by edematous swelling in the head-throat-brisket region,swollen and haemorrhagic lymph nodes and nu- ...

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基因在类风湿关节炎中的相对重要性:丹麦全国双胞胎人群的历史性队列研究

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英国医学杂志中文版 2002年第4期5卷 186-188页

作者： anders J Svendsen Niels V Holm Kirsten Kyvik Per Hyltoft petersen Peter Junker 张文 DepartmentofRheumatology DepartmentofRadiotherapyandClinicalOncology DanishTwinRegistry DepartmentofPublicHealth DepartmentofClinicalChemistry

目的 :了解环境和基因在类风湿关节炎(RheumatoidArthritis ,RA)发病中的相对重要性。设计 :将丹麦双胞胎登记和出院登记记录相结合进行历史性队列研究。同时用丹麦全国性死亡登记来评价本研究的完整性。背景 :以两组人群为基础的全国... 详细信息

目的 :了解环境和基因在类风湿关节炎(RheumatoidArthritis ,RA)发病中的相对重要性。设计 :将丹麦双胞胎登记和出院登记记录相结合进行历史性队列研究。同时用丹麦全国性死亡登记来评价本研究的完整性。背景 :以两组人群为基础的全国双胞胎队列研究。参加者 :本研究共向 3 73 3 8例双胞胎发送了有关风湿性疾病的问卷。对本人自报患有RA的患者进行了临床检查并从医院的病历记录中得到证实。主要的测定结果 :同卵双生和异卵双生中与先证者RA的共患率。结果 :问卷回应率为 84 7%。经证实有 13例同卵双生和 3 6例异卵双生者患有RA。在同卵双生对中无共患RA者 ,在异卵双生对中共患RA的有 2对。以捕获 -再捕获方法确认的可能性为78 3 %。与先证者共患率在同卵双生中为 0 (95 %可信区间为 0～ 2 4 7) ,在异卵双生为 8 8(1 9～2 3 7)。结论

关键词：基因类风湿关节炎相对重要性丹麦双胞胎人群发病因素

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美国国立神经疾病和卒中研究所-加拿大卒中网血管性认知障碍统一标准

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国际脑血管病杂志 2007年第1期15卷 4-24页

作者： Vladimir Hachinski Costantino Iadecola Ron C.petersen Monique M.Breteler David L.Nyenhuis Sandra E.Black William J.Powers Charles DeCarli Jose G.Merino Raj N.Kalaria Harry V.Vinters David M.Holtzman Gary A.Rosenberg Martin Dichgans John R.Marler Gabrielle G.Leblanc 李焰生（译）俞羚（译）高枚春（译）张静芳（译）熊昕丽（译）邹静（译）林岩（译）潘元美（译）不详上海交通大学医学院附属仁济医院神经内科 200127

背景和目的：1／3的个体会罹患卒中和（或）痴呆，而且，除卒中或痴呆外，2倍于此数的人会出现认知障碍。常用的卒中量表并不能评价认知功能，而痴呆的诊断标准则集中在认知障碍的晚期阶段，且在很大程度上偏向Alzheimer病（AD）的诊断... 详细信息

背景和目的：1／3的个体会罹患卒中和（或）痴呆，而且，除卒中或痴呆外，2倍于此数的人会出现认知障碍。常用的卒中量表并不能评价认知功能，而痴呆的诊断标准则集中在认知障碍的晚期阶段，且在很大程度上偏向Alzheimer病（AD）的诊断。尚缺乏普遍公认的标准用于识别和描述存在认知障碍的个体，尤其是在早期阶段，而且特别是与血管因素有关的认知障碍或血管性认知障碍。方法：美国国立神经疾病和卒中研究所（MINDS）与加拿大卒中网（CSN）召集临床诊断、流行病学、神经心理学、脑影像学、神经病理学、试验模型、生物标记物、遗传学和临床试验方面的研究人员，为血管性认知障碍的描述和研究推荐一些最低限度的常用的临床和研究标准。结果：将这些讨论的结果发表于此。结论：一个统一标准的制定代表着使用、确认和改进过程中的第一步。使用相同的标准将有助于在认知障碍的早期阶段识别患者，使不同的研究具有可比性，并且通过整合知识来加速研究进展的步伐。

关键词： Bimwanger病CADASIL 综合征脑梗死脑血管病痴呆遗传学缺血腔隙性梗死白质疏松神经心理学卒中血管性痴呆

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Performance and calibration of quark/gluon-jet taggers using 140 fb^(-1) of pp collisions at √s=13 TeV with the ATLAS detector

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Chinese Physics C 2024年第2期48卷 1-25页

作者： G. Aad B. Abbott K. Abeling N.J. Abicht S.H. Abidi A. Aboulhorma H. Abramowicz H. Abreu Y. Abulaiti B.S. Acharya C. Adam Bourdarios L. Adamczyk S.V. Addepalli M.J. Addison J. Adelman A. Adiguzel T. Adye A.A. Affolder Y. Afik M.N. Agaras J. Agarwala A. Aggarwal C. Agheorghiesei A. Ahmad F. Ahmadov W.S. Ahmed S. Ahuja X. Ai G. Aielli A. Aikot M. Ait Tamlihat B. Aitbenchikh I. Aizenberg M. Akbiyik T.P.A. Åkesson A.V. Akimov D. Akiyama N.N. Akolkar K. Al Khoury G.L. Alberghi J. Albert P. Albicocco G.L. Albouy S. Alderweireldt M. Aleksa I.N. Aleksandrov C. Alexa T. Alexopoulos F. Alfonsi M. Algren M. Alhroob B. Ali H.M.J. Ali S. Ali S.W. Alibocus M. Aliev G. Alimonti W. Alkakhi C. Allaire B.M.M. Allbrooke J.F. Allen C.A. Allendes Flores P.P. Allport A. Aloisio F. Alonso C. Alpigiani M. Alvarez Estevez A. Alvarez Fernandez M. Alves Cardoso M.G. Alviggi M. Aly Y. Amaral Coutinho A. Ambler C. Amelung M. Amerl C.G. Ames D. Amidei S.P. Amor Dos Santos K.R. Amos V. Ananiev C. Anastopoulos T. Andeen J.K. anders S.Y. Andrean A. Andreazza S. Angelidakis A. Angerami A.V. Anisenkov A. Annovi C. Antel M.T. Anthony E. Antipov M. Antonelli F. Anulli M. Aoki T. Aoki J.A. Aparisi Pozo M.A. Aparo L. Aperio Bella C. Appelt A. Apyan N. Aranzabal S.J. Arbiol Val C. Arcangeletti A.T.H. Arce E. Arena J-F. Arguin S. Argyropoulos J.-H. Arling O. Arnaez H. Arnold G. Artoni H. Asada K. Asai S. Asai N.A. Asbah J. Assahsah K. Assamagan R. Astalos S. Atashi R.J. Atkin M. Atkinson H. Atmani P.A. Atmasiddha K. Augsten S. Auricchio A.D. Auriol V.A. Austrup G. Avolio K. Axiotis G. Azuelos D. Babal H. Bachacou K. Bachas A. Bachiu F. Backman A. Badea P. Bagnaia M. Bahmani A.J. Bailey V.R. Bailey J.T. Baines L. Baines O.K. Baker E. Bakos D. Bakshi Gupta V. Balakrishnan R. Balasubramanian E.M. Baldin P. Balek E. Ballabene F. Balli L.M. Baltes W.K. Balunas J. Balz E. Banas M. Bandieramonte A. Bandyopadhyay S. Bansal L. Barak M. Barakat E.L. Barberio D. Barberis M. Barbero M.Z. Barel K.N. Barends T. Barillari M-S. Barisits T. Barklow P. Baron D.A. Baron Mo The ATLAS Collaboration

The identification of jets originating from quarks and gluons,often referred to as quark/gluon tagging,plays an important role in various analyses performed at the Large Hadron Collider,as Standard Model measurements and searches for new particles decaying to quarks often rely on suppressing a large gluon-induced *** paper describes the measurement of the efficiencies of quark/gluon taggers developed within the ATLAS Collaboration,using √s=13 TeV proton–proton collision data with an integrated luminosity of 140 fb^(-1) collected by the ATLAS *** taggers with high performances in rejecting jets from gluon over jets from quarks are studied:one tagger is based on requirements on the number of inner-detector tracks associated with the jet,and the other combines several jet substructure observables using a boosted decision tree.A method is established to determine the quark/gluon fraction in data,by using quark/gluon-enriched subsamples defined by the jet *** in tagging efficiency between data and simulation are provided for jets with transverse momentum between 500 GeV and 2 TeV and for multiple tagger working points.

关键词： ATLAS JET QUARK GLUON TAGGING

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