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Dimensions of human ejaculated spermatozoa in Papanicolaou- stained seminal and swim-up smears obtained from the Integrated Semen Analysis System （ISAS）

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Asian Journal of Andrology 2010年第6期12卷 871-879页

作者： Giuseppe Bellastella Trevor G. Cooper MarinaBattaglia anda strose Inma Torres Barbara Hellenkemper Caries Soler Antonio A. Sinisi Centre of Reproductive Medicine and Andrology of the University Miinster D-48149 Germany Department of Clinical and Experimental Medicine and Surgery Endocrinology and Medical Andrology Section SecondaUniversita＇di Napoli Napoli 80131 ltaly Department of Life Science and Engineering Saxiom Hogescholen Enschede 7500 Netherlands Tahe Fertilidad Murcia Avd. Europa 11-13 Murcia CP 30007 Spain Department of Functlonal Blology and Phystcal Anthropology University of Valbncia Valbncia 46112 Spain

Objective measurements are required for computer-aided sperm morphometric analysis （CASMA） machines to distinguish normal from abnormal sperm heads. The morphometric characteristics of spermatozoa in 72 samples of semen and of spermatozoa from 72 other semen samples after swim-up were quantified by the semi-automated Integrated Sperm Analysis System （ISAS） computer-aided system, which measured the sperm head parameters length （L）, width （W）, area （A）, perimeter （P）, acrosomal area （Ac）, and the derived values L/W and P/A. For each man a homogeneous population of distributions characterized seminal spermatozoa （7 942 cells： median values L 4.4 μm, W 2.8μm A 9.8 μm^2, P 12.5 μm, Ac 47.5%, L/W 1.57, P/A 1.27）, and there was no significant difference in within- and among-individual variation. Different men could have spermatozoa of significantly different dimensions. Head dimensions for swim-up spermatozoa from different men （4 812 cells） were similar to those in semen, differing only by 2%-5%. The values of L, Wand L/W fell within the limits given by the World Health Organization （WHO）. Although these samples were not biologically matched, linear mixed-effects statistical analyses permitted valid comparison of the groups. A subpopulation of 404 spermatozoa considered to fit the stringent criteria of WHO ‘normal＇ seminal spermatozoa from both semen and swim-up were characterized by median values （and 95% confidence intervals） of L, 4.3 μm （3.8-4.9）, W, 2.9 μm （2.6-3.3）, A, 10.2μm^2 （8.5-12.2）, P, 12.4μm （11.3-13.9）, Ac, 49% （36-60）, L/W, 1.49 （1.32-1.67） and P/A, 1.22 （1.11-1.35）. These median values fall within the 95th centile confidence limits given by WHO, but the confidence intervals for L and W were larger. Although these differences in head dimensions among men and after swim-up could be detected by CASMA, the small differences make it unlikely that technicians would be able to distinguish them. The values could be used as default sperm head values for the CASMA

关键词： male infertility semen sperm head

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Mountant-induced loss of Shorr-stained blue sperm tails

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Asian Journal of Andrology 2010年第2期12卷 278-280页

作者： Barbara Hellenkemper anda strose Trevor G. Cooper Centre of Reproductive Medicine and Andrology of the University Miinster D-48149 Germany Department of Life Science and Engineering Saxion Hogescholen Enschede 7500 Netherlands

It has been reported that Short staining provides additional morphological information on the motility of spermatozoa in semen,by distinguishing between red and blue flagella. With our routine methods （involving mounting slides） we were unable to confirm these observations. The presence of both red- and blue-coloured sperm tails in Short-stained semen smears was apparent,however,if slides were unmounted. Only a very weak association between blue flagellar staining and immotility was observed. Stating whether a mountant was used should be reported.

关键词： Mountant-induced loss Shorr-stained blue sperm tails

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