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Glycosylation-related gene expression in HT29-MTX-E12 cells upon infection by Helicobacter pylori

World Journal of Gastroenterology 2017年第37期23卷 6817-6832页

作者： Michael T Cairns ananya gupta Julie A Naughton Marian Kane Marguerite Clyne Lokesh Joshi Glycoscience Group National Centre for Biomedical Engineering ScienceNational University of Ireland Galway School of Natural Sciences National University of Ireland Galway Conway Institute of Biomolecular and Biomedical Research School of Medicine and Medical Sciences University College Dublin

AIM To identify glycosylation-related genes in the HT29 derivative cell line, HT29-MTX-E12, showing differential expression on infection with Helicobacter pylori(H. pylori).METHODS Polarised HT29-MTX-E12 cells were infected for 24 h with H. pylori strain 26695. After infection RNA was isolated from both infected and non-infected host cells. Sufficient infections were carried out to provide triplicate samples for microarray analysis and for q RTPCR analysis. RNA was isolated and hybridised to Affymetrix arrays. Analysis of microarray data identified genes significantly differentially expressed upon infection. Genes were grouped into gene ontology functional categories. Selected genes associated with host glycan structure(glycosyltransferases, hydrolases, lectins, mucins) were validated by real-time q RT-PCR *** Infection of host cells was confirmed by the isolation of live bacteria after 24 h incubation and by PCR amplification of bacteria-specific genes from the host cell RNA. H. pylori do not survive incubation under the adopted culture conditions unless they associate with the adherent mucus layer of the host cell. Microarray analysis identified a total of 276 genes that were significantly differentially expressed(P < 0.05) upon H. pylori infection and where the fold change in expression was greater than 2. Six of these genes are involved in glycosylation-related processes. Real-time q RT-PCR demonstrated significant downregulation(1.8-fold, P < 0.05) of the mucin MUC20. REG4 was heavily expressed and significantly downregulated(3.1-fold, P < 0.05) upon infection. Gene ontology analysis was consistent with previous studies on H. pylori *** Gene expression data suggest that infection with H. pylori causes a decrease in glycan synthesis, resulting in shorter and simpler glycan structures.

关键词： Glycosylation Adherent mucus Gastric HT29-MTX-E12 H pylori strain 26695 Transcriptomics

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Skeletal dysplasias: A radiographic approach and review of common non-lethal skeletal dysplasias

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World Journal of Radiology 2014年第10期6卷 808-825页

作者： ananya Panda Shivanand Gamanagatti Manisha Jana Arun Kumar gupta Department of Radiodiagnosis All India Institute of Medical Sciences Ansari Nagar New Delhi 110029 India

Skeletal dysplasias are not uncommon entities and a radiologist is likely to encounter a suspected case of dysplasia in his practice. The correct and early diagnosis of dysplasia is important for management of complications and for future genetic counselling. While there is an exhaustive classification system on dysplasias, it is important to be familiar with the radiological features of common dysplasias. In this article, we enumerate a radiographic approach to skeletal dysplasias, describe the essential as well as differentiating features of common non-lethal skeletal dysplasias and conclude by presenting working algorithms to either definitively diagnose a particular dysplasia or suggest the most likely differential diagnoses to the referring clinician and thus direct further workup of the patient.

关键词： Skeletal dysplasia Short limb dwarfism Rhizomelia Radiograph Skeletal survey Review Spondylopepiphyseal dysplasia Multiple epiphyseal dysplasia Achondroplasia Algorithm Approach

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Perfusion computed tomography in renal cell carcinoma

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World Journal of Radiology 2015年第7期7卷 170-179页

作者： Chandan J Das Usha Thingujam ananya Panda Sanjay Sharma Arun Kumar gupta Department of Radiology All India Institute of Medical Sciences

Various imaging modalities are available for the diagnosis, staging and response evaluation of patients with renal cell carcinoma(RCC). While contrast enhanced computed tomography(CT) is used as the standard of imaging for size, morphological evaluation and response assessment in RCC, a new functional imaging technique like perfusion CT(p CT), goes down to the molecular level and provides new perspectives in imaging of RCC. p CT depicts regional tumor perfusion and vascular permeability which are indirect parameters of tumor angiogenesis and thereby provides vital information regarding tumor microenvironment. Also response evaluation using p CT may predate the size criteria used in Response Evaluation Criteria in Solid Tumors, as changes in the perfusion occurs earlier following tissue kinase inhibitors before any actual change in size. This may potentially help in predicting prognosis, better selection of therapy and more accurate and better response evaluation in patients with RCC. This article describes the techniques and role of p CT in staging and response assessment in patients with RCCs.

关键词： Angiogenesis Anti-angiogenic therapy Perfusion computed tomography Renal cell carcinoma

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