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Variation in seed morphometric characters, oil content and azadirachtin content of seeds, in vitro shoot cultures and callus cultures among different populations of Azadirachta indica

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Journal of Forestry Research 2018年第1期29卷 121-127页

作者： Fatima Shirin anamika rai Neelu Singh Genetics and Plant Propagation Division Tropical Forest Research Institute PO RFRC Non-Wood Forest Produce Division Tropical Forest Research Institute PO RFRC

We studied seed morphometric characters, oil content and azadirachtin content in a medicinally important and insect repellent tree species Azadirachta indica ***. Ten populations were selected from two central Indian states of Madhya Pradesh and Chhattisgarh and twenty candidate plus trees were selected from each population based on tree attributes. Azadirachtin content was estimated in seeds, in vitro shoots and callus cultures through HPLC. Significant variation（p ／ 0.05） was observed in seed morphometric characters, oil content and azadirachtin content among the ten populations. Maximum value for 100 seed weight was obtained in seeds of raigarh（30.51 g） which was on par with seeds of Bargi（28.37 g）and Chhatarpur（26.36 g）. The ratio of highest kernel to seed was also obtained in seeds of raigarh（60.11%）.Kernels from Chhatarpur population recorded the highest oil content（24.20%）, which was equal to that in Sihore（23.75%） and Katni（21.75%）. We recorded highly significant variation in azadirachtin content in seed samples,in vitro shoot samples and callus cultures. Maximum azadirachtin was present in the seeds of Gwalior（0.882%）which was on par with azadirachtin content in seeds of Chhatarpur, Katni, Sihore, Shahdol and *** azadirachtin in the in vitro shoots was present in the cultures of Gwalior（0.218%） which was statistically on par with Sihore（0.176%）. Similarly, in callus cultures of nodal segments maximum azadirachtin was recorded in the Gwalior population（0.033%） which was statistically on par with Sihore（0.028%）. The trees of Gwalior and Sihore populations can be produced in large numbers through vegetative propagation to obtain seeds with higher azadirachtin content.

关键词： Azadirachtin In vitro Seed morphometric characters Populations Variation

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Enhanced Lead Uptake by an Association of Plant and Earthworm Bioaugmented with Bacteria

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Pedosphere 2018年第2期28卷 311-322页

作者： anamika das jabez w.osborne Department of Biosciences Vellore Institute of Technology Vellore 632014 (India)

Lead(Pb) is recorded as the second most hazardous pollutant of the *** cases of Pb bioremediation has been reported using single biosystem,but very few reports are available in biological approaches using multi-biosystems to achieve an enhanced bioremoval of *** present study evaluated the capacity of a unique association of Pennisetum purpureum,a hyperaccumulator plant,and Lumbricus terrestris(earthworm)bioaugmented with a Pb-resistant bacterium,obtained from an industrially contaminated site and identified as isolate VITMVCJ1 Klebsiella variicola,to bioremediate *** Pb-resistant gene was amplified in the bacterial isolate *** study was conducted for 60 *** verified that the bioaugmentation process enhanced1)root and shoot length of the plants,2)chlorophyll content of the plants,and 3)biofilm-producing ability of the microbes from the rhizosphere region of the *** total phenolic and flavonoid contents were found to be lower in the plants in the bioaugmented *** study also observed a reduction in the toxic effects of Pb on earthworm and *** earthworm was used to assess the Pb-induced stress syndrome after exposure to sublethal concentrations of Pb in the soil.A reduction in the content of malondialdehyde,a potential biomarker,on exposure to Pb demonstrated the role of the inoculum to alleviate heavy metal-induced stress in *** three symbionts accumulated Pb;Pb was accumulated mainly in the root of the plant,and poorly in the shoot of the plant and body mass of the *** bioaugmentation system exhibited stable and excellent uptake of Pb from the contaminated *** results of the present study suggest the positive effect of the synergistic association of the plant and earthworm with appropriate microbes for the bioremoval of Pb.

关键词： bioaugmentation bioremediation heavy metal hyperaccumulator Klebsiella variicola Lumbricus terrestris malondi-aldehyde Pennisetum purpureum

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Optimal batch shipment policy for an imperfect production system under price-,advertisementand green-sensitive demand

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Journal of Management Analytics 2022年第1期9卷 86-119页

作者： Bibhas C.Giri anamika Dash Department of Mathematics Jadavpur UniversityKolkataIndia

In this paper,we consider an imperfect production-inventory system which consists of a single manufacturer and a single *** manufacturer delivers the order quantity to the retailer in some unequal-sized *** separate the defective items,the retailer performs an error-free screening process after receiving each delivery from the *** in retailer’s inventory is allowed and completely *** customer demand is influenced by the retail price,advertisement frequency and greening level of the *** centralized model and the decentralized model based on a Stackelberg gaming approach are developed to determine optimal pricing,advertising and inventory decisions.A cost-sharing contract between the manufacturer and the retailer is implemented,which enhances the environmental performance,advertisement frequency and profitability of the supply chain *** proposed model is illustrated with a numerical *** analysis for some key parameters is carried out and several managerial insights are also highlighted.

关键词： Imperfect production unequal batch shipment price- advertisementand green-sensitive demand cost-sharing contract

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Comparative response of seedlings of selected native dry tropical and alien invasive species to CO_(2) enrichment

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Journal of Plant Ecology 2009年第2期2卷 69-75页

作者： Purnima raizada anamika Singh A.S.Raghubanshi Ecosystems Analysis Laboratory Centre of Advanced Study in BotanyBanaras Hindu UniversityVaranasi 221005Uttar PradeshIndia Department of Botany Punjab UniversityChandigarh 160014PunjabIndia

Global climate change and ongoing plant invasion are the two prominent ecological issues threatening biodiversity world *** invasive species,Lantana camara and Hyptis suaveolens are the two most important invaders in the dry deciduous forest in *** monitored the growth of these two invasive species and seedlings of four native dry deciduous species(Acacia catechu,Bauhinia variegata,Dalbergia latifolia and Tectona grandis)under ambient(375–395μmol mol^(-1))and elevated CO_(2)(700–750μmol mol^(-1))to study the differential growth response of invasive and native *** Seedlings of all the species were exposed to ambient and elevated CO_(2).After 60 days of exposure,seedlings were harvested and all the growth-related parameters like plant height;biomass of root,stem and leaves;total seedling biomass;R/S ratio;allocation parameters;net assimilation rate(NAR)and relative growth rate(RGR)were *** Findings Biomass,RGR and NAR of all the species increased under elevated CO_(2)but the increase was higher in invasive species and they formed larger seedlings than *** under the CO_(2)-enriched future atmosphere,competitive hierarchies could change and may interfere with the species composition of the invaded area.

关键词： dry deciduous seedlings elevated CO_(2) global change plant invasion

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Enzymatic digestibility of pretreated dewaxed bamboo residues as feedstock for bioethanol production

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Systems Microbiology and Biomanufacturing 2022年第4期2卷 716-722页

作者： Sabeela Beevi Ummalyma Karki Baibhav anamika Singh Institute of Bioresources and Sustainable Development(IBSD) A National Institute Under Department of BiotechnologyGovt.of IndiaTakyelpatImphalManipur 795001India

Due to abundant availability,biofuel production from bamboo residues is gaining *** biomass and its residues are widely available in the north-eastern part of India,which can be sustainably exploited for green energy *** aim of the present study was to evaluate the pretreatments of dewaxed bamboo residues with dilute alkali(NaOH),and dilute acid,and the effect of pretreatment on biomass hydrolysis was performed with a commercial enzyme(Zytex).Results showed that maximum fermentable sugars were 490 mg/g obtained from 10%biomass with 0.6%(w/v)of alkali,and acid pretreated biomass produced 320 mg/g of sugars from 2%(w/v)acid with 10%*** in biomass structure during the pretreatment process are correlated with FTIR,SEM,and component analysis for lignin,cellulose,and *** studies of the hydrolysate showed that the yield of ethanol was 77%of the theoretical maximum at 36 *** indicate the scope of utilization of bamboo residues as substrates for biofuels,and alkaline pretreatment is an effective pretreatment process for bioethanol production.

关键词： Bamboo Pretreatment Enzymatic hydrolysis Bioethanol production

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An overview and experimental analysis of WO_(3)/TiO_(2)composite with enhanced electrochromic properties for smart windows application

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Tungsten 2024年第4期6卷 732-747页

作者： Ayesha Khan Mayur A.Gaikwad Jin Hyeok Kim anamika Kadam The Institute of Science Dr.Homi Bhabha State UniversityNo.15 Madam Cama RoadMumbai400032India Optoelectronics Convergence Research Center and Department of Materials Science and Engineering Chonnam National UniversityGwangju61186South Korea

Internationally,standards for electrochromic(EC)requirements in smart window applications have been established,with ongoing global initiatives aimed at elevating them to highly advanced *** paper covers a comprehensive investigation of the structural,optical,morphological,and electrochemical properties of WO_(3)/TiO_(2)composite ***,an experimental analysis of WO_(3)/TiO_(2)was envisaged by a two-step ***,WO_(3)thin films were hydrothermally deposited onto indium-doped tin oxide-coated glass substrates using an aqueous solution of Na_(2)WO_(4)·2H_(2)O at a pH value of ***,a layer of TiO_(2)was electrodeposited onto the WO_(3)thin films.X-ray diffraction analysis confirmed the successful formation of WO_(3)/TiO_(2)composite films,with the WO_(3)phase exhibiting a hexagonal tunnel *** electron microscopy revealed the formation of porous nanorods(NRs)of WO_(3),uniformly coated with TiO_(2),resulting in a porous morphology of the WO./TiO_(2)*** EC performance of the WO_(3)/TiO_(2)films was thoroughly assessed through cyclic voltammetry and chronoamperometry measurements over a potential window ranging from-2.0 to+1.2 V(versus Ag/AgCl)in a 0.5 mol·L^(-1)LiClO_(4)-PC *** WO_(3)/TiO_(2)composite films exhibited cathodic electrochromism,characterized by a reversible color change from dark blue to *** improved EC performance in comparison to pure WO_(3)films is attributed to enhanced double ion/electron insertion and extraction ***,the WO_(3)/TiO_(2)composite films demonstrated excellent optical modulation properties,with a significant modulation at 633 nm(46.87%).The coloration efficiency reached a high value of 193.25 cm^(2)·C^(-1),indicating their potential for practical EC ***,the WO_(3)/TiO_(2)composite films displayed exceptional EC stability,with no significant degradation observed over 2500 cyclic voltammetry *** superior EC

关键词： Electrochromism WO_(3)/TiO_(2) Stability Smart window

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Identification of groundwater potential in hard rock aquifer systems using Remote Sensing, GIS and Magnetic Survey in Veppanthattai, Perambalur, Tamilnadu

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Journal of Groundwater Science and Engineering 2022年第4期10卷 367-380页

作者： Muthamilselvan A anamika Sekar Emmanuel Ignatius Department of Remote Sensing Bharathidasan UniversityKhajamalai CampusTiruchirappalliTamil Nadu-620023India

Water is an essential natural resource without which life wouldn’t *** study aims to identify groundwater potential areas in Vepapanthattai taluk of Perambalur district,Tamil Nadu,India,using analytic hierarchy process(AHP)*** sensing and magnetic parameters have been used to determine the evaluation indicators for groundwater occurrence under the ArcGIS *** occurrence is linked to structural porosity and permeability over the predominantly hard rock terrain,making magnetic data more relevant for locating groundwater potential zones in the research ***-SW and NW-SE trending magnetic breaks derived from reduction to pole map are found to be more significant for groundwater *** lineaments rose diagram indicates the general trend of the fracture to be in the NE-SW *** normalised criteria weights acquired using the AHP model was used to reclassify the thematic *** a result,the taluk’s low,moderate,and high potential zones cover 25.08%,25.68%and 49.24%of the study area,*** high potential zones exhibit characteristics favourable for groundwater infiltration and storage,with factors as gentle slope of<3°,high lineament densities,magnetic breaks,magnetic low zones as indicative of dykes and cracks,lithology as colluvial deposits and land surface with dense *** depth of the fracture zones was estimated using power spectrum and Euler Deconvolution *** groundwater potential mapping results were validated using groundwater level data measured from the wells,which indicated that the groundwater potential zoning results are consistent with the data derived from the real world.

关键词： Groundwater exploration Remote Sensing and GIS Magnetic data Hard rock terrain Analytical hierarchy process Radially averaged power spectrum

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Transplantation of engineered exosomes derived from bone marrow mesenchymal stromal cells ameliorate diabetic peripheral neuropathy under electrical stimulation

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Bioactive Materials 2021年第8期6卷 2231-2249页

作者： anamika Singh Alok Raghav Parvaiz Ahmad Shiekh Ashok Kumar Biomaterial and Tissue Engineering Group Department of Biological Sciences and BioengineeringIndian Institute of Technology KanpurKanpur208016UPIndia Centre for Nanosciences Indian Institute of Technology KanpurKanpur208016UPIndia Centre for Environmental Sciences and Engineering Indian Institute of Technology KanpurKanpur208016UPIndia

Diabetic peripheral neuropathy(DPN)is a long-term complication associated with nerve dysfunction and uncontrolled *** spite of new drug discoveries,development of effective therapy is much needed to cure ***,we have developed a combinatorial approach to provide biochemical and electrical cues,considered to be important for nerve *** derived from bone marrow mesenchymal stromal cells(BMSCs)were fused with polypyrrole nanoparticles(PpyNps)containing liposomes to deliver both the cues in a single delivery *** developed DPN rat model and injected intramuscularly the fused exosomal system to understand its long-term therapeutic *** found that the fused system along with electrical stimulation normalized the nerve conduction velocity(57.60±0.45 m/s)and compound muscle action potential(16.96±0.73 mV)similar to healthy control(58.53±1.10 m/s;18.19±1.45 mV).Gastrocnemius muscle morphology,muscle mass,and integrity were recovered after ***,we also observed paracrine effect of delivered exosomes in controlling hyperglycemia and loss in body weight and also showed attenuation of damage to the tissues such as the pancreas,kidney,and *** work provides a promising effective treatment and also contribute cutting edge therapeutic approach for the treatment of DPN.

关键词： Exosomes Bone marrow mesenchymal stromal cells Polypyrrole Diabetes mellitus Diabetic peripheral neuropathy

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Restoration of dysregulated CC chemokine signaling for monocyte/macrophage chemotaxis in head and neck squamous cell carcinoma patients by neem leaf glycoprotein maximizes tumor cell cytotoxicity

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Cellular & Molecular Immunology 2010年第5期7卷 396-408页

作者： Krishnendu Chakraborty anamika Bose Tathagata Chakraborty Koustav Sarkar Shyamal Goswami Smarajit Pal Rathindranath Baral Department of Immunoregulation and Immunodiagnostics Chittaranjan National Cancer InstituteKolkataIndia Unit of Clinical Biochemistry Chittaranjan National Cancer InstituteKolkataIndia

Previous studies have shown that the CC chemokine receptor CCR5 is downregulated on monocyte/macrophage(MO/Mw)surfaces in head and neck squamous cell carcinoma(HNSCC)patients(stage IIIB).Ligands(RANTES,MIP-1a and MIP-1b)of this chemokine receptor were also secreted in lesser quantity from MO/Mw of HNSCC patients in comparison with healthy *** an aim to restore this dysregulated receptor–ligand signaling,we have used neem leaf glycoprotein(NLGP),a novel immunomodulator reported from our *** upregulated CCR5 expression,as evidenced from studies on MO/Mw of peripheral blood from HNSCC patients as well as healthy *** of RANTES,MIP-1a and MIP-1b was also upregulated following NLGP treatment of these cells in ***,NLGP has little effect on the expression of CCR5 and the ligand RANTES in oral cancer *** restored CCR5 receptor–ligand signaling seen in MO/Mw was reflected in improved CCR5-dependent,p38 mitogen-activated protein kinase(MAPK)-mediated migration of MO/Mw after NLGP treatment to a standard *** also induces better antigen presentation and simultaneous costimulation to effector T cells by MO/Mw by upregulating human leucocyte antigen(HLA)-ABC,CD80 and *** addition,NLGP-treated MO/Mw-primed T cells can effectively lyse tumor cells in *** effects of NLGP on monocyte migration and T cell-mediated oral tumor cell killing were further demonstrated in transwell assays with or without CCR5 *** results suggest a new approach in cancer immunotherapy by modulating dysregulated CCR5 signals from MO/Mw.

关键词： CCR5 chemokine head and neck squamous cell carcinoma monocytes neem leaf glycoprotein

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Efficient Microorganism Compost Benefits Plant Growth and Improves Soil Health in Calendula and Marigold

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Horticultural Plant Journal 2017年第2期3卷 67-72页

作者： anamika Sharma Tarak Nath Saha Anju Arora Raghubir Shah Lata Nain Division of Microbiology Indian Agricultural Research Institute Directorate of Floriculture Research Shivaji Nagar

Organic farming needs to be promoted as a means of sustaining soil health and reducing the cost of farming, especially for small *** present study deals with the effect of organic compost, prepared using Efficient Microorganism(EM) consortium and applied along with full or half of the recommended dose of chemical fertilizers, on the growth of Calendula and Marigold plants, soil physico-chemical parameters and soil enzyme activities. Soil enzyme activities were improved with the increase in the rate of EM compost application in both Calendula and Marigold. Carotenoid pigment increased by 46.11% and 12.19% with application of EM compost over the control in Calendula and Marigold flowers respectively. Soil humus, available nitrogen and organic carbon content also increased due to the supplementation of EM compost resulting in better soil fertility. For Calendula, treatment T5(Half dose NPK + EM compost 20 000 kg·hm^(-2)) was found to be the most promising in terms of acid phosphatase(82.63 μg p-Nitrophenyl Phosphate·g^(-1)·h^(-1)), dehydrogenase(10.46 μg Triphenyl Formazan·g^(-1)·d^(-1)) and β-glucosidase(0.30 IU·g^(-1)) activities. In Marigold, treatment C(Half dose NPK + EM compost 5 000 kg·hm^(-2)) was the most promising in terms of amendment in soil enzyme activities.

关键词： calendula marigold EM compost soil fertility plant growth soil enzyme activity soil physico-chemical parameter

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