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Efficiency of Inoculation with Rhizobium in Peanuts(Arachis hypogaea L.)Grown in Brazilian Cerrado Soil

Agricultural Sciences 2019年第7期10卷 948-956页

作者： Salomao Lima Guimaraes Edna Maria Bonfim-Silva analy castilho polizel de souza Bency G.Simeon department of Agricultural and Environmental Engineering Institute of Agricultural Sciences and TechnologyFederal University of Mato GrossoRondonópolisBrazil

In Brazil, the peanut culture has been expanding mainly in the state of São Paulo, responsible for 80% of the national production. It is an edible culture produces oil of excellent quality, contains proteins rich in amino acids that make up to 36% of weight of the grain. Furthermore, it is associated with nitrogen-fixing bacteria. However, the practice of inoculation is not very common in peanuts, being this species considered susceptible to nodularity with a large number of rhizobia. However, known’s that inoculation with selected strains can increase the efficiency of symbiosis and peanut yield. Thus, the present study was proposed to evaluate the development of peanut inoculated with rhizobia and grown in Cerrado’s Oxisol. The experiment was conducted in a greenhouse, in a completely randomized design, five treatments, one cultivar (IAC 505) and four replicates. The treatments were composed of three strains of rhizobia and two controls, one absolute and the other with nitrogen fertilizer (50 mg⋅N⋅dm3). Each experimental plot has been consisted an 8 dm3 pot with three plants. The inoculation was done, applying 3 mL of bacterial broth (108 mL−1 cells) in the root system of each plant. The SPAD index, number and dry weight of nodules, dry mass of shoots and roots, number and weight of grains were analyzed. The response of Peanuts inoculated was significantly, as all inoculated plants showed similar development to nitrogen-fertilized, emphasizing the potential for supplying nitrogen in peanut development.

关键词： Biological Nitrogen Fixation Nodulation Symbiosis

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Study of interaction between metal ions and quercetin

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Food Science and Human Wellness 2018年第3期7卷 215-219页

作者： Taiane souza de castilho Tatiane Brescovites Matias Keller Paulo Nicolini Jaqueline Nicolini Instituto Federal do Parana-IFPR Lacoppi-Laboratorio de Corantes e Processos PiroliticosDepartamento de QuimicaPalmasPRBrazil

A displacement test based on the interaction between the flavonoid quercetin and an excess of metal chloride allows the determination of the binding constant for the reaction between quercetin and Ca2+,Mg^2+and Ni^2+.The values obtained were 2.20±1.77×10^3 for Ca^2+,1.37±0.59×10^3 for Mg^2+and 7.03±1.04×10^4 for Ni^2+,and all interactions showed type 1:1 stoichiometry,as determined by titration and by the method of continuous variations(Job’s method).The complexion effect was observed qualitatively through a colorimetric change in the medium(yellow→neon yellow)and spectroscopically through a bathochromic shift in the absorption band of quercetin in the presence of *** investigation serves as a tool for the development and testing of materials capable of capturing toxic metal ions or favoring the absorption of beneficial ions(in relation to the human metabolism)through the construction of efficient bioorganic *** results reported herein allow understanding of this detection system,indicating the following ascending order of the binding constants(Mg^2+

关键词： Binding constants Quercetin Antioxidant properties

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Effects of Climate and Agricultural Practices on Temporal Progress of Sclerotinia sclerotiorum for Soybean in Brazil

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Agricultural Sciences 2016年第7期7卷 425-438页

作者： Gustavo castilho Beruski André Belmont Pereira David souza Jaccoud-Filho Felipe Fadel Sartori Paulo Cesar Sentelhas department of Biosystems Engineering University of Sao Paulo—ESALQ/USP Piracicaba Brazil department of Soil Science and Agricultural Engineering State University of Ponta Grossa Ponta Grossa Brazil department of Plant Sciences and Crop Protection State University of Ponta Grossa Ponta Grossa Brazil

Currently the soybean crop is affected by the white mold (Sclerotinia sclerotiorum). The disease can reduce the crop yield and quality and decrease the prices of agricultural lands. The aim of the current research was to assess epidemiology of white mold on soybean crop grown at Arapoti, PR, Brazil, utilizing data related to agricultural practices and local meteorological factors. The experiment was conducted in a naturally infested area. The soybean crop was sown on October 18th, 2011. The experimental design adopted herein was a randomized block in a factorial combination with 4 row spacings (0.35, 0.45, 0.60, 0.75 m) and 4 plant populations (150, 200, 250, 300 thousand plants per hectare) and 4 replications. The temporal analysis of the epidemic was evaluated using mathematical models, such as Logistics, Monomolecular and Gompertz, in order to determine the best model that described the progress of the disease as a function of local meteorological elements. For the incidence data it has been shown that both logistic and monomolecular models were those that were best fitted to the experimental data. For severity, the best model related to the experimental data was the logistic one. Either for incidence or for severity, air temperature was considered to be the environmental factor most affecting the progress of the disease. The variability in the apparent infection rates of white mold on soybean was not affected by different row spacings and plant populations;therefore, suggesting that macroclimatic variations prevailed in such a fashion to mitigate the effect of cultural practices adopted in the field.

关键词： Meteorological Data Sclerotinia sclerotiorum (Lib.) de Bary Glycine max Row Spacing Plant Population Epidemiology

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Essential Oils from Mentha viridis(L).*** Mentha pulegium L.:Cytogenotoxic Effects on Human Cells

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American Journal of Plant Sciences 2017年第6期8卷 1423-1437页

作者： Lucilene Fernandes Silva Maria das Gracas Cardoso Paulo Sérgio castilho Preté Maria Luisa Teixeira David Lee Nelson Maísa Lamounier Magalhaes Vanuzia Rodrigues Fernandes Ferreira Rafaela Vieira souza Luana Isac Soares Silvana Marcussi departamento de Química Universidade Federal de LavrasCampus UniversitárioLavrasMinas GeraisBrasil departamento de Química Universidade Federal dos Vales do Jequitinhonha e MucuriCampus UniversitárioDiamantinaMinas GeraisBrasil departamento de Ciência dos Alimentos Universidade Federal de LavrasCampus UniversitárioLavrasMinas GeraisBrasil

The essential oils from Mentha viridis (L). L. and Mentha pulegium L. were studied to assess their inhibitory potential on phospholipase from snake venoms and to determine their cytogenotoxic action on human cells. These essential oils were able to inhibit the breakdown of phospholipids induced by venoms of snakes of the Bothrops genus. Both oils presented hemolytic activity, although the Mentha viridis (L). L. oil induced hemolysis only at the highest concentrations (14.6 and 29 μL·mL-1). The essential oil from M. viridis induced 3.9;8.6 and 16.2 times greater damage to human leukocyte DNA than that observed with the positive control (100 μg·μL-1 doxorubicin) at concentrations of 0.25;0.5 and 1.0 μL·mL-1, respectively. A similar effect was observed for the oil from M. pulegium (2.1, 2.5 and 15.8 times greater damage). The results extend the characterization of these essential oils and demonstrate their potential use in industries.

关键词： Essential Oils Mentha viridis(L).L. Mentha pulegium L. Hemolysis Comet Assay

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