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Initial Development of Cowpea Bean Fertilized with Natural Phosphate in the Brazilian Cerrado Soil

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American Journal of Plant Sciences 2018年第7期9卷 1381-1390页

作者： Edna Maria Bonfim-Silva Denise César Soares paula Caroline Lima Silva ana paula Alves Barreto Damasceno Tonny José Araújo da Silva Helon Hébano de Freitas Sousa Department of Agricultural and Environmental Engineering Institute of Agricultural Sciences and Technology Federal University of Mato Grosso Cuiabá Brazil

Cowpea, string Bean, or Macassar bean [Vigna unguiculata (L.) Walp] presents a short life cycle, low water requirement, develops in soils of low fertility, and has the ability to fix nitrogen from the air. In the Cerrado, the oxisols occupy practically all the flat to soft-wavy areas with little reserve of nutrients for the plants, in general, they are soils with great limitations of fertility. A source of alternative phosphate fertilization is the use of reactive natural phosphates. The reactive natural phosphate of Bayóvar presents intermediate solubility between the sources of soluble phosphates and the natural phosphates. In this context, the objective was to evaluate the initial development of Cowpea beans fertilized with the natural Bayóvar phosphate used for cultivation of the Brazilian Cerrado Oxisol. The experimental design was completely randomized, with six doses of phosphorus (0, 100, 200, 300, 400, 500 mg·dm-3) using natural Bayovar phosphate as a source in four replications. The species used in this study was the legume cowpea bean [Vigna unguiculata (L.) Walp] cultivar Tumucumaque. The treatments were applied using the natural phosphate Bayóvar as a source of phosphorus (29% of P2O5). At 33 DAS (days after sowing), the variable number of leaves of the Cowpea bean was analyzed and at 40 DAS, the variables plant height, SPAD chlorophyll index (Soil Plant analysis Development) and stem diameter were analyzed. For all variables analyzed there was a significant effect. The initial development of the cowpea bean cultivated in the Brazilian Cerrado Oxisol was significantly influenced by the Bayóvar natural phosphate fertilization with the best phosphorus (P2O5) doses in the range of 200 to 350 mg·

关键词： Vigna unguiculata (L.) Walp Phosphate of Bayóvar Oxisol

More Transporters,More Substrates:The Arabidopsis Major Facilitator Superfamily Revisited

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Molecular Plant 2019年第9期12卷 1182-1202页

作者： Maria Nino-Gonzalez Esther Novo-Uzal Dale N.Richardson Pedro M.Barros paula Duque Instituto Gulbenkian de Ciencia 2780-156 OeirasPortugal Genomics of Plant Stress Unit ITQB NOVA-Instituto de Tecnologia Quimica e Biologica Antonio XavierUniversidade Nova de Lisboa2780-157 OeirasPortugal

The Major Facilitator Superfamily(MFS)is ubiquitous in living organisms and represents the largest group of secondary active membrane *** plants,significant research efforts have focused on the role of specific families within the MFS,particularly those transporting macronutrients(C,N,and P)that constitute the vast majority of the members of this *** MFS families remain less explored,although a plethora of additional substrates and physiological functions have been ***,the lack of a systematic approach to analyzing the MFS as a whole has obscured the high diversity and versatility of these ***,we present a phylogenetic analysis of all annotated MFS domaincontaining proteins encoded in the Arab/dops/s fA?a//ana genome and propose that this superfamily of transporters consists of 218 members,clustered in 22 *** reviewing the available information regarding the diversity in biological functions and substrates of Arab/dops/s MFS members,we provide arguments for intensified research on these membrane transporters to unveil the breadth of their physiological relevance,disclose the molecular mechanisms underlying their mode of action,and explore their biotechnological potential.

关键词： Arabidopsis thaliana membrane transport MFS transporters phylogenetic analysis

Caboxymetylcellulose/Gelatin Blends Loaded with Piroxicam: Preparation, Characterization and Evaluation of in Vitro Release Profile

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Journal of Encapsulation and Adsorption Sciences 2013年第4期3卷 99-107页

作者： Vivia Buzzi Marli Brudner Theodoro Maciel Wagner Giovana C. Bazzo ana paula Testa Pezzin Denise Abatti Kasper Silva Campus of Joinville Joinville-Brazil Campus Universitário University of Region of Joinville—UNIVILLE Joinville Brazil

Piroxicam is a non-steroidal anti-inflammatory drug (NSAID) exhibiting analgesic and antipiretic properties and widely used in the management of chronic diseases. Associated with these use, there are registers of adverse reactions. Microparticle formulations in hydrogel matrix can be used to form a semi-permeable barrier which enables their actions and can reduce the effects. This work presents a study on the effect of gelatin/carboxymethyl cellulose (Gel/CMC) semi-IPN matrix composition on the retention and kinetic behavior of releasing this drug. The microparticles were obtained through the emulsion-crosslinking method using 23 factorial planning and the piroxicam was added as solid particles. In order to characterize the interaction between matrix-active agent and quantification of the drug, the following techniques were applied: SEM, DSC and XRD. SEM micrographs revealed microparticles with regular and spherical shape and that in some compositions the drug is partially absorbed and not encapsulated. Beyond that DSC and XRD analyses indicate that the piroxicam remained in the matrixes, maintains the same crystalline form. The factorial planning analysis indicated that matrix obtained a maximum encapsulation efficiency (EE%) of 10.64% and became possible to create a response surface graph using a EE% as answer. In addition to this, release kinetics analyses demonstrated that the release process seems to be governed by distinctly kinetic models, considering the composition of the sample. In some samples the release can be driven by Fickinianan diffusion, others by anomalous transport or swelling.

关键词： Piroxicam Gelatin Carboxymethyl Cellulose

Contraception Knowledge, Attitudes and Practices of Adolescents at Risk of Pregnancy in Northeastern Brazil

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Open Journal of Obstetrics and Gynecology 2014年第6期4卷 300-308页

作者： Gilka Paiva Oliveira Costa Giulia Paiva Oliveira Costa Monica de paula Farias ana Cristina Pinheiro Fernandes de Araújo Federal University of Paraíba Joao Pessoa(PB)Brazil Federal University of Rio Grande do Norte Joao Pessoa(PB)Brazil

Background: Contraceptive methods (CM) are important resources in the prevention of adolescent pregnancy. This study aimed to investigate adolescent knowledge, attitudes and practices related to contraception. Study Design: A cross-sectional and exploratory study was conducted with 7th to 9th grade adolescents in northeastern Brazil. Their knowledge, attitudes, and contraceptive method use were analysed by gender, age and sexual initiation groups. Results: A sexual debut had been experienced by 21% (n = 120) of all respondents (n = 570). The majority of the respondents who had experienced a sexual debut were male (73.3%, n = 88), their mean age was 15 years (SD = 1.46), and 49.3% (n = 59) never used any CM. The participants displayed low knowledge and unfavourable and ambivalent attitudes concerning contraception. Conclusion: Adolescents initiate sexual intercourse with knowledge and attitudes that limit the use of CM, which makes them more at risk of pregnancy. Further research that will be representative of the specific population in need of interventions is necessary.

关键词： Adolescent Contraception Pregnancy Attitudes

Effects of Sodium Diclofenac on the Distribution of Fos Protein in Central Amygdala and Lateral Hypothalamus during Experimental Tooth Movement in Rats

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World Journal of Neuroscience 2014年第2期4卷 183-189页

作者： ana paula R. Novaes Amanda C. Desiderá Glauce C. Nascimento Christie R. A. Leite-Panissi Department of Morphology Physiology and Basic Pathology Ribeirao Preto Dentistry School University of Sao Paulo Ribeirao Preto Brazil Psychobiology Graduate Program School of Philosophy Science and Literature of Ribeirao Preto University of Sao Paulo Ribeirao Preto Brazil

This study evaluated whether the administration of a NSAID, sodium diclofenac, can promote alterations in the expression of Fos protein in central amygdala (CEA) and the lateral hypothalamus (LH) after 6 h of experimental tooth movement with a controlled force of 70 g, applied to the superior central incisors of rats. Adult male rats were anesthetized and divided into four groups: Control, no orthodontic appliance (OA);OA activated with 70 g;OA activated with 70 g and pretreated with diclofenac sodium (5 mg/kg, intramuscular);and diclofenac sodium alone. Six hours after the onset of the experiment the rats were reanesthetized and perfused with 4% paraformaldehyde. The brains were removed and fixed, and sections containing the CEA and LH were processed for Fos protein immunohistochemistry. The results show that in the control group, intramuscular injection of a ketamine/xylazine mixture did not induce IR-Fos cells in the CEA or LH. However, in the 70 g group, IR-Fos was the strongest observed

关键词： Amygdala Lateral Hypothalamus Fos Protein Orthodontic Movement Sodium Diclofenac

Viability of Lactobacillus acidophilus La-5 in the Presence of Williopsis saturnus vat. suaveolens

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Journal of Food Science and Engineering 2013年第4期3卷 176-186页

作者： Sabrina Neves Casarotti Aline Teodoro de paula Marilia Goncalves Cattelan Catharina Calochi Pires de Carvalho ana Lucia Barretto Penna Department of Food Engineering and Technology Sao Paulo State University Sao Jose do Rio Preto SP 15054000 Brazil

Maintaining the viability of probiotic microorganisms from production to consumption has long been a technological challenge for the food industry. The objectives of this study were to evaluate the in vitro interaction between Lactobacillus acidophilus La-5 and Williopsis saturnus var. suaveolens and the effect of this yeast on acidification kinetics, viability ofLactobacillus acidophilus and post-acidification in fermented milk during refrigerated storage at 5℃. The in vitro study showed a positive interaction between the acid cell free-supernatant （CFS） ofprobiotic bacteria La-5 and the yeast. The addition of W. saturnus var. suaveolens increased the fermentation time due to consumption of the organic acids produced by L. acidophilus. During the refrigerated storage of the samples, the presence of the yeast increased the viability of L. acidophilus and reduced post-acidification. However, the mechanism of such interaction of bacteria and yeast is not fully understood.

关键词： Probiotic lactic acid bacteria yeast W. saturnus var. suaveolens fermented dairy products viability

Probabilistic Distributions for <i>Acacia Mearnsii</i>De Wild Total Height and the Influence of Environmental Factors

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Journal of Applied Mathematics and Physics 2014年第3期2卷 1-10页

作者： Carlos Roberto Sanquetta Alexandre Behling Allan Libano Pelissari ana paula Dalla Corte Sylvio Péllico Netto Augusto Arlindo Simon Department of Forest Science University Federal of Paraná Curitiba Brazil

This paper discusses the hypothesis of height distribution on a forest stand of Acacia mearnsii De Wild, known as black wattle. It remains constant at varied growing environments and, in addition, they are not influenced by age factor. The Wakeby equation was applied. The research was carried out in a black wattle stand at varied age levels and over two different agroecological regions where plantations are found: Serra do Sudeste and Encosta do Sudeste, Rio Grande do Sul State, Brazil. It was observed that as the age rises there is an increase in the stand total height;while the number of trees decreases for the lower classes, it increases for the upper ones. This resulted in lengthening of the curve tail to the left and mode shift to the right, generating negative asymmetrical curves. Two types of height distribution were found: the sharp increase of probability in a specific class and some similar probabilities in successive classes. The distribution curves between the cultivation areas were statistically different and therefore the height distribution was dependent of environment.

关键词： Wakeby Distribution Forest Stand Age Cultivation Area Forest Structure

Influence of Photoperiod over Morphometric and Hematological Parameters of Juvenile Piracanjubas （Brycon orbygnianus）

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Journal of Agricultural Science and Technology(B) 2016年第5期6卷 350-359页

作者： Monica Rodrigues Ferreira Machado] Eliane Aparecida de Andrade Estefania de Souza Andrade Daniella Aparecida de Jesus paula Julia Alvarenga Oliveira Adriano Carvalho Costa ana paula Peconick Luis David Solis Murgas Department of Biological Science Federal University of Goias (UFG) Campus Cidade Universitaria CEP 75801-615 JataiGoias Brazil Department of Veterinary Medicine Cdmpus Universitario Federal University of Lavras (UFLA) Lavras Minas Gerais CEP3 7200-000 Brazil Department of Animal Science Federal Institute of Rio Verde (IFR V) Rio Verde Goias CEP 75901-970 Brazil

The aim of this study was to evaluate morphometric and hematological parameters of juveniles Piracanjubas treated with two distinct photoperiod. Twenty nine fish with 100 g, in a factorial design were performed in 18 h light：6 h dark （18L：6D） and 6L：lSD two different photoperiod, and morphometric and hematological parameters were evaluated at 45 d and 90 d after photoperiod treatment. Benzocaine 1% was used for euthanasia. Fish were weighed and measured, so as visceral fat, liver and gonads. Hematocrit, total count of white cells, lymphocytes and neutrophils were counted. Results showed that there was no significant relationship between bodyweight and standard length. Significant interaction （P = 0.00018） had been found between the fat index （FI） and gonado-somatic index （GSI） and the photoperiod and time. At 90 d, the fish kept in 18L：6D had a lower rate of fat than the fish kept in 6L：18D （P 〈 0.05）. The GSI was higher in the group 6L.＇18D compared with the 18L：6D （P 〈 0.005）. There was no interaction between photoperiod and period of treatment for total leukocyte count, however with 90 d, the total leukocyte count was decreased （P 〈 0.05）. Moreover, decrease of the hematocrit from 58.1% to 46.9% and an increase of lymphocytes from 31.5% to 43.5% had been found in fish kept in 18L：6D after 90 d （P 〈 0.05）. Fish kept in 6L：ISD presented an increase in segmented neutrophils and lymphocytes （P 〈 0.05）. It is concluded that photoperiod maintained at 6L： 18D for Piracanjubas permits a greater gonadal development and an amount of immune cells over the photoperiod maintained in 18L： 6D.

关键词： Circadian rhythm fish maturity cellular immune response photoperiod.

Horizontally root fractured teeth with pulpal vitality——two case reports

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World Journal of Radiology 2016年第12期8卷 928-932页

作者： Luciano Silva Pamella Alvares Jose Alcides Arruda Leni Veronica Silva Cleomar Rodrigues ana paula Veras Sobral Marcia Silveira Universidade de Pernambuco Department of Dental Radiology and Imaging Faculdades Integradas da Uniao Educacional do Planalto CentralSIGA Area Especial para Indústria

This case study reports the successful outcome of horizontal root fractures of two different patients, which took place in permanent incisors. Report 1 describes a case of a 29-year-old patient who suffered a mandibular trauma affecting mainly the lower central incisors, caused by a car accident. A panoramic radiograph was taken right after the accident and showed a horizontal root fracture in the middle third of tooth 42, which went untreated. Report 2 illustrates a case of a 17-year-old male patient who searched for orthodontic therapy and the periapical radiograph showed horizontal root fracture in tooth 11 caused by a previous trauma, which went untreated as well. There was healing through the reestablishment of pulp activity and dental coloration without professional intervention.

关键词： Horizontal root fractures Pulpal vitality Periapical radiograph