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Screenning of Chlamydia trachomatis Infection among Women Attending Outpatient Clinic of Infertility

Open Journal of Obstetrics and Gynecology 2015年第11期5卷 600-607页

作者： Heloisa Lopes Lavorato Natália Prearo Moco Laura Fernandes Martin ana gabriela pontes santos anaglória pontes Marli Teresinha Cassamassimo Duarte Márcia Guimaraes da Silva Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil Department of Pathology Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil Department of Nursing Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil

Objective: The goal of this study was to determine the prevalence of C. trachomatis in women diagnosed with infertility attending the Outpatient Clinic of Infertility from Botucatu Medical School, UNESP, Brazil. Patients and Methods: This molecular study enrolled a total of 112 women. Among these patients, 62 presented primary infertility while 50 presented secondary infertility. The criteria for eligibility included women who were: reproductive-aged;no prior report of seroconversion for HIV;no antibiotic or vaginal cream used in the preceding 30 days;and abstinence from sexual intercourse for 72 hours before the visit. The women were submitted to a gynecological examination and cervical samples were collected with an endocervical cytobrush for molecular analysis of C. trachomatis. Results: The prevalence of chlamydial infection was 8% with similar prevalence between primary (8.1%) and secondary (8.0%) infertility. Conclusion: Considering the asymptomatic nature of chlamydial infection and its association with tubal factor infertility, there is a pressing need to incorporate the screening of C. trachomatis infection as part of the routine investigation for infertility. The early diagnostic by screening can minimize complications and reduce Public Health costs with Assisted Reproductive Technology.

关键词： Chlamydial Infection Infertility Molecular Diagnosis

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Early detection of tomato bacterial canker by reflectance indices

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Information Processing in Agriculture 2022年第2期9卷 184-194页

作者： gabriela Cordon Carolina Andrade Lucia Barbara ana Maria Romero Universidad de Buenos Aires Consejo Nacional de Investigaciones Cientı´ficas y Te´cnicasInstituto de Investigaciones Fisiolo´gicas y Ecolo´gicas Vinculadas a la Agricultura(IFEVA)Facultad de Agronomı´aBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aA´rea de Educacio´n AgropecuariaBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aCa´tedra de Fitopatologı´aBuenos AiresArgentina

Early detection of infected asymptomatic plants could prevent the dissemination of tomatobacterial canker throughout the crop and, thus, reduce associated economic losses. Reflectance spectroscopy has been proposed for the detection of diseases in plants, mainlycaused by foliar fungal pathogens. In contrast, bacterial canker is a vascular disease thatdoes not produce visible foliar symptoms until it is too late to avoid contagion to otherplants. The effects of the disease on the conductivity of the xylem have an impact onthe water content of the leaves;initially, a subtle effect that cannot be assessed with thenaked eye but can be evaluated by reflectance spectroscopy. The objective of this workwas to identify indices, based on the reflectance spectral signature of the plants, for thedetection of tomato plants affected by bacterial canker prior to the appearance of symptoms. To verify that the proposed methodology is applicable to a broad range of tomatogenotypes, two experiments were carried out with different tomato cultivars grown in potsand another on the ground in a greenhouse. Plants were inoculated with the pathogenicbacteria and the reflectance spectrum of the apical leaflet of two leaves per plant wereobtained before symptoms expression;several spectral indices were calculated from thereflectance spectra. Three indices, of the shortwave-infrared zone, allowed the detectionof bacterial canker inoculated plants in a fast and non-destructive way, up to one weekbefore symptoms were visible: Normalized Difference Water Index (NDWI), Simple Ratioof Water Index (SRWI) and Water Index 1 180 (WI1180). Our research demonstrates, forthe first time, the usefulness of spectral indices sensitive to water content for the identifi-cation of diseased plants. These indices arise as a promising tool for the early detection ofvascular plant diseases, that could be integrated into sustainable management plans.

关键词： Clavibacter michiganensis subsp. michiganensis Bacterial wilt Reflectance indices Solanum lycopersicum L

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Comparison of grain protein profiles of Brazilian cowpea (Vigna unguiculata) cultivars based on principal component analysis

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Food Production, Processing and Nutrition 2022年第1期4卷 172-182页

作者： Tuany Camila Honaiser gabriela Barbosa Rossi Maurisrael de Moura Rocha ana Carolina Maisonnave Arisi Food Science and Technology Department CAL CCA UFSCFederal University of Santa CatarinaRod.Admar GonzagaFlorianópolisSanta Catarina134688034-001Brazil Embrapa Meio-Norte Caixa Postal 001TeresinaPiauí64008-780Brazil

This study aims to compare the grain protein profile of four Brazilian cowpea cultivars (BRS Aracê, BRS Itaim, BRS Pajeú, and BRS Xiquexique) by two-dimensional electrophoresis (2-DE) and principal component analysis (PCA). 2-DE efficiently separate cowpea protein profiles, showing high homogeneity among the four cultivars. In addition, the principal component analysis indicated that there is a difference in abundance of proteins among the cultivars. The cultivars BRS Aracê and BRS Xiquexique, both biofortified in iron and zinc, were separated from the cultivars BRS Itaim and BRS Pajeú. These results demonstrate that protein profiles can be used to discriminate cowpea varieties.

关键词： Vigna 2-DE PCA Legumes

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Aeration and sawdust application effects as structural material in the bioremediation of clayey acid soils contaminated with diesel oil

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International Soil and Water Conservation Research 2018年第3期6卷 253-260页

作者： gabriela Menta Alvim Patrícia Procópio pontes Department of Environmental Science and Technology Federal Centre for Technological Education of Minas Gerais525330-421169 Belo HorizonteBrazil

Soil contamination, due to the leakage of petroleum or its by-products, is a reason for concern nowadays. Technologies, such as bioremediation, have been developed to remove these pollutants from the environment. This paper aims to evaluate the bioremediation of clayey acid soils contaminated with diesel oil, comparing the effect of aeration use and the addition of different sawdust ratios (5% and 10%) as bulking material. This research was developed in three phases, with experiments of (a) natural attenuation (exclusively natural processes), (b) sawdust-amended soil and (c) aeration combined with sawdust addition. The manometric respirometry test was applied to monitor CO2 production during biodegradation experiments and sodium hydroxide (NaOH) was used as an absorbent solution. The contamination of the samples was carried out by the addition of diesel oil (5% w/w (gg-1)) into the soil. The results indicated that natural attenuation presented a slower bioremediation process when compared to the treatments with sawdust addition. The addition of 5% of sawdust was beneficial to the microbial activity of the soil, but a proportion above 5% caused a negative effect on the samples, reducing the microbial activity. Statistical analysis (Kruskal-Wallis test) showed significant oil removals on experiments with the combination of sawdust and aeration. The aeration was considered the main agent in increasing oil biodegradation in the soil. The degradation rate reactions followed a zero-order linear model. The highest contaminant degradation rate was observed in the samples with 5% of sawdust and aeration (24.79 mgO2 kg-1dry soil day-1), which also improved microbial activity and diesel oil removal.

关键词： Bioremediation Diesel oil Contaminated soil Sawdust Aeration

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Application of Bioinspired Superhydrophobic Surfaces in Two-phase Heat Transfer Experiments

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Journal of Bionic Engineering 2017年第3期14卷 506-519页

作者： Emanuele Teodori ana Sofia Moita Miguel Moura Pedro pontes Antonio Moreira Yuan Bai Xinlin Li Yan Liu IN+- Center for Innovation Technology and Policy Research Instituto Superior T~enico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portuga Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University Changchun 130022 China

This paper addresses the potential to use Lotus leafbioinspired surfaces in applications involving heat transfer with phase change, namely pool boiling and spray impingement. Besides describing the role of bioinspired topographical features, using an innovative technique combining high-speed visualization and time-resolved infrared thermography, surface durability is also addressed. Water is used for pool boiling and for spray impingement systems （simplified as single droplet impact）, while HFE7000 is used in a pool boiling cooler for electronic components. Results show that surface durability is quickly compro- mised for water pool boiling applications, as the chemical treatment does not withstand high temperatures （T 〉 100 ℃） during long time intervals （3 h - 4 h）. For HFE7000 pool boiling （depicting lower saturation temperature - 34 ℃）, heat transfer en- hancement is governed by the topography. The regular hierarchical pattern of the bioinspired surfaces promotes the heat transfer coefficient to increase up to 22.2%, when compared to smooth surfaces, while allowing good control of the interaction mechanisms until a distance between micro-structures of 300 gm- 400 Ixm. Droplet impingement was studied for surface temperatures ranging between 60 ℃ - 100 ℃. The results do not support the use of superhydrophobic surfaces for cooling applications, but reveal great potential for other applications involving droplet impact on heated surfaces （e.g. metallurgy in- dustry）.

关键词： bioinspired surfaces surface micro-patterning two-phase heat transfer time resolved infrared thermography

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Bubble Dynamics and Heat Transfer on Biphilic Surfaces:Experiments and Numerical Simulation

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Journal of Bionic Engineering 2020年第4期17卷 809-821页

作者： Pedro pontes Ricardo Cautela Emanuele Teodori ana Sofia Moita anastasios Georgoulas António Luís Nobre Moreira Moreira IN+-Center for Innovation Technology and Policy ResearchDepartment of Mechanical EngineeringInstituto Superior TécnicoUniversidade de LisboaAv.Rovisco Pais1049-001 LisboaPortugal ASML Holding N.V. De Run 65015504 DRVeldhovenThe Netherlands Department of Exact Sciences and Engineering Portuguese Military AcademyR.Gomes Freire2031169-203 LisboaPortugal Advanced Engineering Centre School of ComputingEngineering and MathematicsCockcroft BuildingLewes RoadUniversity of Brighton BN24GJUK

Wettability is known to play a major role in enhancing pool boiling heat *** this context bioinspired surfaces can bring significant advantages in pool boiling *** work addresses a numerical investigation of bubble growth and detachment on a biphilic surface pattern,namely in a superhydrophobic region surrounded by a hydrophilic *** characteristics resemble bioinspired solutions explored in our research group,mainly considering the main topographical *** numerical approach is intended to provide additional information to an experimental approach,allowing to obtain temperature,pressure and velocity fields in and around the bubble,which help to describe bubble *** model was validated based on experimental data obtained with extensive image processing of synchronized high-speed video and high-speed thermographic *** results obtained here clearly evidence that combining enhanced direct numerical simulations with high-resolution transient experimental measurements is a promising tool to describe the complex and intricate hydrodynamic and heat transfer phenomena governing pool boiling on heated biphilic surfaces.

关键词： bioinspired biphilic surfaces bubble dynamics two-phase heat transfer time resolved infrared thermography CFD model

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Medical management of symptomatic severe aortic stenosis in patients non-eligible for transcatheter aortic valve implantation

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Journal of Geriatric Cardiology 2020年第11期17卷 704-709页

作者： gabriela Silvia Gheorghe Andreea Simona Hodorogea Andrei Cristian Dan Gheorghe Ioan Tiberiu Nanea ana Ciobanu University of Medicine and Pharmacy Carol Davila BucharestProf.Dr.Th.Burghele HospitalBucharestRomania

1 Transcatheter aortic valve implantation in symptomatic severe aortic stenosis: where do we stand? Aortic stenosis occurs in 2%-9% of patients over the age of 65, the most common cause being degenerative.^([1,2]) The preferred treatment in symptomatic severe aortic stenosis(SAS) is surgical aortic valve replacement(SAVR), but in the elderly, the surgical risk can be greater than the benefit.([3]).

关键词： Angiotensin-converting enzyme inhibitors Beta-blockers Calcium channel blockers Diuretics Symptomatic severe aortic stenosis Transcatheter aortic valve implantation

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Arylated analogues of cypronazole: fungicidal effect and activity on human fibroblasts. Docking analysis and molecular dynamics simulations

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Natural Products and Bioprospecting 2022年第1期12卷 107-120页

作者： Natividad Herrera Cano Sebastian A.Andujar Cristina Theoduloz Daniel A.Wunderlin ana N.Santiago Guillermo Schmeda-Hirschmann Ricardo D.Enriz gabriela E.Feresin Instituto de Biotecnología Facultad de IngenieríaUniversidad Nacional de San JuanCONICET-CCT San JuanAv.Libertador General San Martín1109(O)5400 San JuanArgentina Facultad de Química Bioquímica y Farmacia-IMIBIO-SL(CONICET)Universidad Nacional de San LuisChacabuco 9155700 San LuisArgentina Laboratorio de Cultivo Celular Facultad de Ciencias de la SaludUniversidad de TalcaCasilla 7473460000 TalcaChile ICYTAC CONICET and Universidad Nacional de CórdobaFacultad de Ciencias QuímicasDepartamentoQuímica OrgánicaCiudad UniversitariaBv.Juan Filloy s/n5000 CórdobaArgentina INFIQC CONICET and Universidad Nacional de CórdobaFacultad de Ciencias QuímicasDepartamento Química OrgánicaCiudad UniversitariaHaya de La Torre S/N5000 CórdobaArgentina Laboratorio de Química de Productos Naturales Instituto de Química de Recursos NaturalesUniversidad de TalcaCasilla 7473460000 TalcaChile

Triadimefon(TDM)and cyproconazole(CPZ)are two triazoles widely used as *** azoles were synthe-sised starting from commercial TDM and *** compounds were evaluated against phytopathogenic filamentous fungi,including Aspergillus fumigatus(AF),***(AN),***(AU),***(AJ),***(AT),Fusarium oxyspo-rum and Botrytis cinerea isolated from grapevine in the province of San Juan,*** of the synthesised compounds(1-(Biphenyl-4-yloxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)butan-2-one,1;2-(Biphenyl-4-yl)-3-cyclopropyl-1-(1H-1,2,4-triazol-1-yl)butan-2-ol,3;3-Cyclopropyl-2-(4’-fluorobiphenyl-4-yl)-1-(1H-1,2,4-triazol1-yl)butan-2-ol,4)presented remarkable in vitro fungicidal properties,with better effects than TDM and CPZ on some of the target *** was assessed using human lung fibroblasts *** 1,with IC50 values of 389.4μM,was less toxic towards MRC-5 human lung fibroblasts than commercial TDM(248.5μM)and CPZ(267.4μM).Docking analysis and molecular dynamics simulations suggest that the compounds present the same interaction in the binding pocket of the CYP51B enzyme and with the same amino acids as *** derivatives investigated could be considered broad-spectrum but with some selectivity towards imperfect fungi.

关键词： Azoles Antifungal cytotoxicity Conformational and electronic analysis

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Patient Frailty Can Increase the Risk of Acute Kidney Injury after Cardiac Surgery: Pilot Study

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World Journal of Cardiovascular Surgery 2022年第10期12卷 245-255页

作者： Sergio Soto-Hopkins José Antonio Sanchez-Lopez Erick Trujillo-Magallón Ron Leder ana gabriela Gallardo-Hernandez Unidad Médica de Alta Especialidad en Cardiología Centro Médico Nacional Siglo XXI Mexico City M&#233xico IEEE Engineering in Medicine and Biology Mexico City M&#233xico Unidad de Investigación Médica en Enfermedades Metabólicas Centro M&#233dico Nacional Siglo XXI Mexico City M&#233xico

Background: Acute kidney injury (AKI) is a severe common postoperative complication of cardiac surgery (CS). It increases the risk of mortality by up to 80%. Therefore, it is essential to have preoperative risk evaluation tools. Frailty is a marker of deterioration of physiologic systems and may be associated with AKI. Purpose: The study aimed to determine the utility of frailty as a predictor of AKI after CS. Method: We enrolled 91 patients undergoing CS with cardiopulmonary bypass to determine if they had frailty before surgery and were associated with postoperative AKI. The diagnosis of postoperative AKI was based on the serum creatinine criteria of the Acute Kidney Injury Network classification up to 7 days following CS. Results: The incidence of postoperative AKI was 62% in the frail group and 21% in the non-frail group. Frailty was associated with a higher risk of AKI (relative risk [RR] = 3.00, 95% CI 1.56 - 5.77, p = 0.00). In regression models, there were associations between frailty and postoperative AKI. Conclusion: This study demonstrated that frailty could be a predictor for post-CS AKI. Therefore, frailty assessment should become an essential part of the preoperative evaluation to help the anesthesiologist to estimate the surgical risk and develop preoperative and transoperative strategies to preserve the renal function and improve the cardiac surgery outcome.

关键词： Cardiac Surgery Heart-Lung Machine Acute Kidney Injury Frailty Cardiopulmonary Bypass

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Particular evolution in a 72-year-old diabetic patient with acute coronary syndrome

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Journal of Geriatric Cardiology 2018年第7期15卷 523-526页

作者： gabriela S Gheorghe ana Clobanu Ioan T Nanea Andreea S Serban Mihaela R Mititelu Department of Internal Medicine and Cardiology "Theodor Burghele" Clinical Hospital "Carol Davila " University of Medicine and Pharmacy Bucharest Romania Nuclear Medicine Department "Dr. Carol Davila " Central Emergency Military Clinical Hospital Bucharest Romania

Diabetes mellitus is an important risk factor for coronary atherosclerosis and many patients present extensive coronary stenosis at ***,in patients with diabetes,endothelial and microvascular dysfunction also participate in chronic and acute myocardial *** the majority of diabetic patients with myocardial infarction have angiographic evidence of significant coronary artery disease,they can also experience myocardial infarctions with non-obstructive coronary arteries (MI-NOCA).[1] The mechanisms leading to myocardial necrosis in these cases are heterogeneous and patients often present atypical evolution,making the diagnostic process and the choice of optimal treatment difficult.

关键词：糖尿病症候群动脉粥样硬化急性进化风险因素机能障碍心肌

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