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Determination of Paraquat in Several Commercially Available Types of Rice

Food and Nutrition Sciences 2018年第12期9卷 1368-1375页

作者： Thais Lopes Lima Maria Aparecida Nicoletti Camila Munhoz Gabriel Ramos De Abreu Julia Zaccarelli Magalhaes Esther Lopes Ricci Ricci Paula A.Faria Waziry Júlia Nathalia Alves da Costa ana carolina nascimento antonio AndréRinaldi Fukushima Universidade Sao Judas Tadeu-R.Taquari Sao Paulo/SPBrazil Departamento de Farmacia.Faculdade de Ciencias Farmaceuticas.Universidade de Sao Paulo Sao Paulo/SPBrazil Instituto de Ciencias da Saude Universidade Presbiteriana MackenzieRua da ConsolacaoSao Paulo/SPBrazil Departamento de Patologia Faculdade de Medicina Veterinaria e ZootecniaUniversidade de Sao PauloSao Paulo/SPBrazil New Southeastern University Fort LauderdaleFloridaUnited States of America Veterans Affairs Medical CenterMiamiFloridaUSA Faculdade Sao Bento-Largo de Sao Bento s/no-CentroSao Paulo-SPSao Paulo/SPBrazil

Introduction: Paraquat (1,1’-dimethyl-4,4’-bipyridine-dichloride) is a herbicide used to combat weeds and improve crop productivity. Its acute toxicity can cause fatal poisoning in humans and animals and several studies have shown a strong correlation between paraquat and Parkinson’s disease. Objective: To determine the concentration of paraquat in different types of rice in Brazil. Method: Paraquat quantification is based on a complexation reaction with sodium dithionite, which generates a blueish color compound, whose absorbance was read in a spectrophotometer at the 600 nm wavelength. Results: Five samples were found to be above the maximum allowed limit, while one sample had a concentration in the upper allowed limit. The method employed did not allow quantification of black rice. Conclusion: There is a need for identification of the type of pesticide used in each examined crop as well as their respective dates of remission in order to improve the safety of agricultural practices.

关键词： Paraquat Herbicide Rice

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Are the cerrado and the seasonal forest woody floras assembled by different processes despite their spatial proximity?

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Journal of Plant Ecology 2018年第5期11卷 740-750页

作者： Raquel carolina Miatto Marco antonio Batalha Department of Botany Federal University of São CarlosPO Box 67613565-905São CarlosBrazil Department of Biology University of São Paulo14040-901Ribeirão PretoBrazil

Aims The Brazilian cerrado occupies land that could be occupied by seasonal forest,given current climatic conditions and their spatial *** has been identified as one of the main determinants of cerrado and forest *** tested whether cerrado and seasonal forest woody floras were assembled by different *** postulated that soil nutrient availability would account for dif-ferences in the functional and phylogenetic structure of the cerrado and the seasonal *** In 1005×5 m plots distributed along cerrado and seasonal forest patches in south-eastern Brazil,we sampled five individuals with a basal diameter≥3 cm from 127 species and measured seven of their functional traits(plant height,basal area,tortuosity,leaf size,specific leaf area,leaf dry matter content,and leaf toughness).We constructed a phylogenetic tree and calculated the pairwise mean functional-phy-logenetic distances(MFPDs),an approach that accounts for functional and phylogenetic information both separately and *** also sampled soils to a depth of 5 cm in each plot and had their chem-ical and physical properties *** related the MFPD to soil properties and compared MFPD between cerrado and forest *** Findings Phylogenetic distances were higher than functional distances in both cerrado and seasonal forest communities,suggesting trait convergence in both *** of the importance given to functional or phylogenetic information,most of the com-munities in the cerrado and in the seasonal forest fell within the null expectation,implying either that multiple assembly processes can occur simultaneously along the gradient of soil fertility,or that not all important traits were *** was related to soil nutrient status when only functional distances were *** this case,MFPD was lower in the cerrado than in the forest,indi-cating that soil nutrient availability influenced plant traits,with the low-nutrient soils in

关键词： competition functional traits environmental filter nutrient limitation phylogeny savanna

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Behavior of CD44 Receptors in Mammary Tumors of Dogs

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Open Journal of Veterinary Medicine 2012年第2期2卷 48-51页

作者： Georgia MMagalhaes ana carolina TSilveira Danisio PMunari antonio CAlessi Department of Veterinary Pathology Sao Paulo State UniversitySao PauloBrazil Department of Animal Science Sergipe Federal UniversitySergipeBrazil

CD44 is a cell adhesion molecule closely related to tumor progression in humans. In canine mammary tumors, little information is available about this molecule. The aim of this study was to analyze, by immunohistochemistry, the behavior of this molecule in canine mammary tumors with or without the presence of metastasis. The dogs were divided in groups without metastasis (G1) and with metastasis (G2, with subsets A—original neoplasia and B—metastatic neoplasia). Tumors were classified according to the World Health Organization (WHO) criteria. The cells were counted whereby the plasma membrane and/or cytoplasm are stained. There was a significant increase in the number of cells immunostained for CD44 in the metastastic masses (G2B) as compared to groups G1 and G2A. It is concluded that in metastatic mass there was a significant increase in CD44 receptors, probably important for biology of the mammary tumor of dogs.

关键词： Dogs Oncology Mammary Tumors

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Production and Characterization of New Fibrinolytic Protease from Mucor subtillissimus UCP 1262 in Solid-State Fermentation

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Advances in Enzyme Research 2015年第3期3卷 81-91页

作者： Thiago Pajeu nascimento Amanda Emmanuelle Sales Camila Souza Porto Romero Marcos Pedrosa Brandao Galba Maria Campos Takaki Jose antonio Couto Teixeira Tatiana Souza Porto ana Lucia Figueiredo Porto Center for Biological Sciences Federal University of PernambucoRecifeBrazil Department of Morphology and Animal Physiology Federal Rural University of PernambucoRecifeBrazil Center for Research in Environmental Sciences Catholic University of PernambucoRecifeBrazil Institute for Biotechnology and Bioengineering Centre of Biological EngineeringUniversity of MinhoCampus of GualtarBragaPortugal Academic Unit of Garanhuns Federal Rural University of PernambucoGaranhunsBrazil

Fibrinolytic enzymes have received attention regarding their medicinal potential for thrombolytic diseases, a leading cause of morbidity and mortality worldwide. Various natural enzymes purified from animal, plant and microbial sources have been extensively studied. The aim of this work was to produce fibrinolytic protease by solid state fermentation using agro industrial substrates. Rhizopus arrhizus var. arrhizus UCP 1295 and Mucor subtillissimus UCP 1262 filamentous fungi species isolated from soil of Caatinga-PE, Brasil, were used as producer microorganisms. Wheat bran was shown to be the best substrate for the production of the enzyme and by using a 23 full factorial design the main effects and interactions of the quantity of the substrate wheat bran, moisture and temperature on the fibrinolytic enzyme production and protease were evaluated. The best results for fibrinolytic and protease activities, 144.58 U/mL and 48.33 U/mL, respectively, were obtained with Mucor subtillissimus UCP 1262 using as culture medium 3 g wheat bran, 50% moisture at a temperature of 25°C for 72 hours. The optimum temperature for the produced enzyme was 45°C and most of its original activity was retained after being subjected to 80°C for 120 min. The protease activity was enhanced by K+, Ca+ and Mn+;but with Cu+ there was an inhibition. The specificity to chromogenic substrate and the inhibition by PMSF indicates that it is a chymotrypsin-like serine protease. Presented results suggest that this enzyme produced by solid-state fermentation is an interesting alternative as a candidate for thrombolytic therapy.

关键词： Mucor Enzyme Protease Fibrinolytic Wheat

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Rheological and Morphological Properties of Composites Based on Polylactide and Talc

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材料科学与工程（中英文B版） 2013年第11期3卷 695-699页

作者： Talita F. Cipriano ana Lucia Nazareth da Silva antonio Henrique Monteiro da Fonseca Thome da Silva anaMaria Furtado de Sousa Gustavo Monteiro da Silva Christine Rabello do nascimento Instituto de Macromol~culas Professora Eloisa Mano (IMA/UFRJ) Rio de Janeiro 21945-970 Brasil Departamento Agricola e Meio Ambiente (UFF) Niteroi 24210-240 Brasil Instituto de Quimica (IQ/UERJ) 20550-013 Brasil Programa de Engenharia Metalurgica (PEMM/COPPE/UFRJ) Rio de Janeiro 21941-908 Brasil

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Vitamin D and Secondary Hyperparathyroidism in HIV Infected Patients Taking Antiretroviral Therapy

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World Journal of AIDS 2014年第4期4卷 430-437页

作者： Huylmer Lucena Chaves Henrique Pires Moreira Hayato Augusto Hossoe Correa William Browne de Oliveira Machado Rian Brito Teles Lucigleyson Ribeiro do nascimento Janedson Baima Bezerra Filho Camilla Camuza Coelho Rabelo Queiroz Danilo Goncalves Nobrega ana carolina Vasconcelos Moreira Maria carolina Nunes Albano de Meneses Fabricio de Maicy Bezerra Melissa Soares Medeiros Medicine School UNICHRISTUSFortalezaBrazil Fortaleza General Hospital FortalezaBrazil Sso Jose Hospital of Infectious Diseases FortalezaBrazil

Objective: Due to the lack of studies assessing hypovitaminosis D and secondary hyperparathyroidism in Brazilian HIV-infected population, especially in the northeastern population, this study aimed to determine the profile of these conditions in patients infected with HIV and its correlation with immuno-virological, sociodemographic data and associated comorbidities. Methods: Comparison studies were obtained from routine clinical samples of HIV infected patients submitted for 25-OH Vitamin D, PTH and alkaline phosphatase determination. Results: A total of 78 patients were included, 42 (53.8%) males, mean age 45.7 years. Antiretroviral regimens most used in this study were Zidovudine/Lamivudine/Efavirenz 17.9%, Tenofovir/Lamivudine/Efavirenz 17.9%,Tenofovir/Lamivudine/Atazanavir-r 15.4%. The mean value CD4 count was 592.1 ± 247.2 cells/mm3, CD8 cell count was 1026.5 ± 467.3 cells/mm3, mean detectable viral load was 2220 ± 15703 copies and CD4/CD8 ratio was 0.63 ± 0.33. A total of 34 vitamin D dosages were collected with 41.2% representing sufficient amount and 58.8% insufficient. Alkaline Phosphatase (ALP) dosage was elevated in 49.3% (N=35) of the patients. Parathormone (PTH) was elevated in 18% (N = 11). Among patients with elevated PTH levels, 81.9% had elevated levels of ALP (p = 0.01). In the group of patients with high levels of ALP, 45.7% had a CD4 count 3 (p = 0.02). There was no significant difference in vitamin D related to gender (p = 0.21), age (p = 0.23), CD4 count (p = 0.26), suppressed viral load (p = 0.44) or blood glucose (p = 0.45). Conclusions: This study evidenced a high prevalence of Vitamin D insufficiency in Northeast Brazil, which suggests HIV infection correlation. A high prevalence of Hyperparathyroidism was detected and related with inflammatory condition persistence and low CD4 count. We suggest improve vitamin D follow up and measurements in this population with better CD4 count control to avoid future osteoarticular complications of HIV

关键词： HIV Hyperparathyroidism Vitamin D Antiretroviral Therapy

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GOECP/SEOR radiotherapy guidelines for small-cell lung cancer

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World Journal of Clinical Oncology 2021年第3期12卷 115-143页

作者： Felipe Couñago carolina de la Pinta Susana Gonzalo Castalia Fernández Piedad Almendros Patricia Calvo Begoña Taboada antonio Gómez-Caamaño JoséLuis López Guerra Marisa Chust Joséantonio GonzálezFerreira anaÁlvarez González Francesc Casas Department of Radiation Oncology Hospital Universitario Quirónsalud MadridHospital La LuzUniversidad Europea de MadridMadrid 28223MadridSpain Department of Radiation Oncology Hospital Universitario Ramón y CajalMadrid 28034Spain Department of Radiation Oncology Hospital Universitario La PrincesaMadrid 28006Spain Department of Radiation Oncology GenesisCare MadridMadrid 28043Spain Department of Radiation Oncology Hospital General UniversitarioValencia 46014Spain Department of Radiation Oncology Hospital Clínico Universitario Santiago de CompostelaSantiago de Compostela 15706Spain Department of Radiation Oncology Hospital Universitario Virgen del RocíoSevilla 41013Spain Department of Radiation Oncology Fundación Instituto Valenciano de OncologíaValencia 46009Spain Department of Radiation Oncology GenesisCare-SpainSevilla 41092Spain Department of Radiation Oncology HGU Gregorio MarañónMadrid 28007Spain Department of Radiation Oncology Thoracic UnitHospital ClinicBarcelona 08036Spain

Small cell lung cancer(SCLC)accounts for approximately 20%of all lung *** main treatment is chemotherapy(Ch).However,the addition of radiotherapy significantly improves overall survival(OS)in patients with non-metastatic SCLC and in those with metastatic SCLC who respond to *** cranial irradiation reduces the risk of brain metastases and improves OS in both metastatic and non-metastatic *** 5-year OS rate in patients with limited-stage disease(non-metastatic)is slightly higher than 30%,but less than 5%in patients with extensive-stage disease(metastatic).The present clinical guidelines were developed by Spanish radiation oncologists on behalf of the Oncologic Group for the Study of Lung Cancer/Spanish Society of Radiation Oncology to provide a current review of the diagnosis,planning,and treatment of *** guidelines emphasise treatment fields,radiation techniques,fractionation,concomitant treatment,and the optimal timing of Ch and ***,we discuss the main indications for reirradiation in local recurrence.

关键词： Small cell lung cancer Chemotherapy Hyperfractionated radiation therapy Prophylactic brain irradiation Brain metastases Reirradiation

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Development of a Ready-to-Use PSMA-11 Kit Formulation and Biological Evaluation of Binding Affinity

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Advances in Molecular Imaging 2024年第2期13卷 7-22页

作者： Maria Renata Valente Brandão Freire Natália Marchesan Bexiga Marco antonio Stephano João Ezequiel Oliveira Carlos Roberto Jorge Soares ana Cláudia Camargo Miranda Anna carolina de Alcântara Barbosa Marycel Figols de Barboza Laura Fernanda Garcia Luiz Felipe S. Teixeira Maria Helena Marumo Bellini Natanael Gomes da Silva Luiza Mascarenhas Balieiro Joel Mendes Elaine Bortoleti de Araújo Centro de Radiofarmcia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil Departamento de Tecnologia Farmacutica Faculdade de Cincias Farmacuticas (USP) Cidade Universitria So Paulo Brasil Centro de Biotecnologia Instituto de Pesquisas Energticas e Nucleares (IPEN) Cidade Universitria So Paulo Brasil Departamento de Medicina Nuclear do Hospital Israelita Albert Einstein So Paulo/SP Brasil

Introduction: 68Ga-PSMA-11 is considered the gold standard in detection of micro and oligometastases in advanced prostate cancer, being used for therapeutic planning, as well as, potentially, for evaluating response to treatment. The development of ready-to-use lyophilized kit of PSMA-11 adds quality and safety to the routine use of this radiopharmaceutical and represents a pharmacotechnical challenge as it must preserve the integrity and specificity of the ligand. Methods: PSMA-11 kit formulation was proposed, considering radiolabeling parameters and the preservation of the peptide during the lyophilization process, using mannitol as an excipient. Critical temperature characterization studies were carried out using DSC equipment and the freeze-drying process was developed. The direct radiolabeling conditions were evaluated and standardized using 68Ge/68Ga generator eluate from two different manufacturers (ITG and Eckert & Ziegler). The radiochemical purity was evaluated by TLC and HPLC. Biological evaluation was carried out with lyophilized PSMA-11 to demonstrate the integrity of the peptide and preservation of biological activity after the lyophilization process. Results: Based on critical temperature characterization studies, the freeze-drying cycle was designed to reach a freezing temperature of around −40˚C and primary drying at 2˚C. Using 20 mg of mannitol, an intact and elegant lyophilized cake was obtained. PSMA-11 lyophilized kit was directly labeled with 68Ga eluate from 68Ge/68Ga GMP generators (ITG and Eckert & Ziegler) resulting in % RP > 95% at pH 4.0 to 4.5. The results obtained from in vitro and in vivo biological competition studies confirmed the preservation of PSMA-11 affinity for the receptor after lyophilization. Conclusion: A lyophilized formulation (Kit) of PSMA-11 was successfully obtained, which preserved the integrity and biological activity of the peptide and guaranteed radiolabeling efficiency.

关键词： 68Ga-PSMA-11 Prostate-Specific Membrane Antigen (PSMA) Ready-to-Use Kit GMP-Grade Generators Metastatic Prostate Cancer PET Imaging

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Fluctuating temperature modifies heat-mortality association around the globe

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The Innovation 2022年第2期3卷 65-72页

作者： Yao Wu Bo Wen Shanshan Li antonio Gasparrini Shilu Tong Ala Overcenco Ales Urban Alexandra Schneider Alireza Entezari ana Maria Vicedo-Cabrera Antonella Zanobetti Antonis analitis Ariana Zeka Aurelio Tobias Barrak Alahmad Ben Armstrong Bertil Forsberg CarmenÍñiguez Caroline Ameling César De la Cruz Valencia ChristoferÅström Danny Houthuijs Do Van Dung Dominic Royé Ene Indermitte Eric Lavigne Fatemeh Mayvaneh Fiorella Acquaotta Francesca de’Donato Francesco Sera Gabriel Carrasco-Escobar Haidong Kan Hans Orru Ho Kim Iulian-Horia Holobaca Jan Kyselý Joana Madureira Joel Schwartz Klea Katsouyanni Magali Hurtado-Diaz Martina SRagettli Masahiro Hashizume Mathilde Pascal Micheline de Sousa Zanotti Stagliorio Coélho Noah Scovronick Paola Michelozzi Patrick Goodman Paulo Hilario nascimento Saldiva Rosana Abrutzky Samuel Osorio Tran Ngoc Dang Valentina Colistro Veronika Huber Whanhee Lee Xerxes Seposo Yasushi Honda Michelle L.Bell Yuming Guo Department of Epidemiology and Preventive Medicine School of Public Health and Preventive MedicineMonash UniversityMelbourne 3004Australia Climate Air Quality Research UnitSchool of Public Health and Preventive MedicineMonash UniversityMelbourne 3004Australia Department of Public Health Environments and SocietyLondon School of Hygiene&Tropical MedicineLondon WC1E 7HTUK Centre for Statistical Methodology London School of Hygiene&Tropical MedicineLondon WC1E 7HTUK Centre on Climate Change&Planetary Health London School of Hygiene&Tropical MedicineLondon WC1E 7HTUK Shanghai Children’s Medical Centre Shanghai Jiao Tong UniversityShanghai 200025China School of Public Health Institute of Environment and Human HealthAnhui Medical UniversityHefei 230032China Center for Global Health Nanjing Medical UniversityNanjing 211166China School of Public Health and Social Work Queensland University of TechnologyBrisbane 4000Australia National Agency for Public Health of the Ministry of Health Labour and Social Protection of the Republic of MoldovaChisinau MD-2009Republic of Moldova Institute of Atmospheric Physics Czech Academy of SciencesPrague 14100Czech Republic Faculty of Environmental Sciences Czech University of Life SciencesPrague 16500Czech Republic Institute of Epidemiology Helmholtz Zentrum M€unchen-German Research Center for Environmental HealthNeuherberg 85747Germany Faculty of Geography and Environmental Sciences Hakim Sabzevari UniversitySabzevar ***Iran Institute of Social and Preventive Medicine University of BernBern 3012Switzerland Oeschger Center for Climate Change Research University of BernBern 3012Switzerland Department of Environmental Health Harvard T.H.Chan School of Public HealthHarvard UniversityBostonMA 02115USA Department of Hygiene Epidemiology and Medical StatisticsNational and Kapodistrian University of AthensAthens 11527Greece Institute for Environment Healthand SocietiesBrunel University LondonLondon UB83PNUK Institute of Environmental Assessment and

Studies have investigated the effects of heat and temperature variability(TV)on ***,few assessed whether TV modifies the heat-mortality *** on daily temperature and mortality in the warm season were collected from 717 locations across 36 *** was calculated as the standard deviation of the average of the same and previous days’minimum and maximum temperatures.

关键词： deviation modif heat

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大气二氧化氮与每日总死亡率、心血管和呼吸系统疾病死亡率的短期关联:398个城市的多中心分析

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英国医学杂志中文版 2021年第8期24卷 435-442页

作者：孟夏刘聪(校) 陈仁杰郑湃(译) 阚海东(校) Francesco Sera ana Vicedo-Cabrera Ai Milojevic Maria Guo Yuming Tong Shilu Micheline de Sousa Zanotti Stagliorio Coelh Paulo Hilario nascimento Saldiva Eric Lavigne Patricia Matus Correa Nicolas Valdes Ortega Samuel Osorio Garcia Jan Kysely Ales Urban Hans Orru Marek Maasikmets Jouni J K Jaakkola Niilo Ryti Veronika Huber Alexandra Schneider Klea Katsouyanni Antonis analitis Masahiro Hashizume Yasushi Honda Chris Fook Sheng Ng Baltazar Nunes João Paulo Teixeira Iulian Horia Holobaca Simona Fratianni Ho Kim Aurelio Tobias Carmeníniguez Bertil Forsberg ChristoferÅström Martina S Ragettli Yue-Liang Leon Guo Shih-Chun Pan Shanshan Li Michelle L Bell Antonella Zanobetti Joel Schwartz Tangchun Wu antonio Gasparrini 复旦大学公共卫生学院、公共卫生安全教育部重点实验室(复旦大学)、卫生部卫生技术评估重点实验室(复旦大学) 中国上海北京大学公共卫生学院复旦大学公共卫生学院不详

目的:采用统一的分析方案,评估全球多个国家/地区的二氧化氮(NO_(2))与总死亡率、心血管和呼吸系统疾病死亡率之间的短期关联。研究设计:采用两阶段的时间序列分析方法、过度离散的广义线性模型和多水平meta分析。研究地点:22个低到高... 详细信息

目的:采用统一的分析方案,评估全球多个国家/地区的二氧化氮(NO_(2))与总死亡率、心血管和呼吸系统疾病死亡率之间的短期关联。研究设计:采用两阶段的时间序列分析方法、过度离散的广义线性模型和多水平meta分析。研究地点:22个低到高收入国家/地区的398个城市。主要结局指标:1973—2018年逐日总死亡人数(6280万人)、心血管疾病死亡人数(1970万人)和呼吸系统疾病死亡人数(550万人)。结果:平均而言,NO_(2)浓度在滞后1天(前1天)每增加10μg/m^(3),会导致总死亡率、心血管和呼吸系统疾病死亡率分别增加0.46%(95%可信区间0.36%~0.57%)、0.37%(0.22%~0.51%)、0.47%(0.21%~0.72%)。在对共污染物(PM_(10)、PM_(2.5)、臭氧、二氧化硫和一氧化碳)进行调整后,这些关联仍然很稳定。所有3种死因的暴露-反应曲线几乎是线性的,没有明显的阈值。在398个城市中,可归因于高过假定零水平的NO_(2)浓度造成的死亡比例为1.23%(95%可信区间0.96%~1.51%)。结论:这项多中心研究提供了关于NO_(2)短期暴露与总死亡率、心血管和呼吸系统死亡风险之间的独立和线性关联的关键证据,说明通过加强NO_(2)的控制和监管限制标准,可获得人群水平的健康收益。

关键词：总死亡率 meta分析总死亡人数死亡风险广义线性模型二氧化氮高收入国家结局指标

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