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Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association b.tween b.cterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis

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World Journal of Gastroenterology 2005年第45期11卷 7142-7147页

作者： Callum b.Pearce Vitaly Zinkevich Iwona b.ech Viera Funjika ana Garcia Ruiz Afraa Aladawi Hamish D Duncan Queen Alexandra Hospital Microb.ology Research Lab.ratory

AIM: To investigate the use of PCR and DGGE to investigate the association b.tween b.cterial translocation and systemic inflammatory response syndrome in predicted severe ***: Patients with b.ochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect b.cterial translocation in b.ood samples collected on d1,3, and 8 after the admission. Standard microb.al b.ood cultures were taken when there was clinical evidence of sepsis or when felt to b. clinically indicated b. the supervising ***: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. b.cteria were detected using PCR in 4 of the 17 collected b.ood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative b.ood cultures. Using DGGE and specific primers, the b.cteria in all b.ood specimens which tested positive for the presence of b.cterial DNA were identified as E ***: Our study confirmed thatunlike traditional microb.ological techniques, PCR can detect the presence of b.cteria in the b.ood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morb.dity and evaluating patients with the disease. Further research using increased numb.rs of patients, in particular those patients with necrosis and sepsis, is required to assess the reliab.lity of PCR and DGGE in the rapid diagnosis of infection in AP.

关键词： Polymerase chain reaction Acutepancreatitis b.cterial translocation

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NEW HYb.ID CONJUGATE GRADIENT METHOD AS A CONVEX COMb.NATION OF LS AND FR METHODS

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Acta Mathematica Scientia 2019年第1期39卷 214-228页

作者： Sne?ana S.DJORDJEVI? Faculty of Technology University of Nis

In this paper, we present a new hyb.id conjugate gradient algorithm for unconstrained optimization. This method is a convex comb.nation of Liu-Storey conjugate gradient method and Fletcher-Reeves conjugate gradient method. We also prove that the search direction of any hyb.id conjugate gradient method, which is a convex comb.nation of two conjugate gradient methods, satisfies the famous D-L conjugacy condition and in the same time accords with the Newton direction with the suitab.e condition. Furthermore, this property doesn't depend on any line search. Next, we also prove that, moduling the value of the parameter t,the Newton direction condition is equivalent to Dai-Liao conjugacy *** strong Wolfe line search conditions are *** glob.l convergence of this new method is *** comparisons show that the present hyb.id conjugate gradient algorithm is the efficient one.

关键词： hyb.id conjugate gradient method convex comb.nation Dai-Liao conjugacy condition Newton direction

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Relaxation of Certain Integral Functionals Depending on Strain and Chemical Composition

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Chinese Annals of Mathematics,Series b 2013年第4期34卷 491-514页

作者： ana Margarida RIb.IRO Elvira ZAPPALE Departamento de Matema'tica and CMA Faculdade de Ci encias e Tecnologia - Universidade Nova de Lisboa D. I. In. Universita’ degli Studi di Salerno

The authors provide a relaxation result in b.×Lq, 1≤q<+∞ as the first step towards the analysis of thermochemical equilib.ia.

The authors provide a relaxation result in b.×Lq, 1≤qanalysis of thermochemical equilib.ia.

关键词： Relaxation Functions of b.unded variation Quasiconvexity

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Reliab.e Content Distrib.tion in P2P Networks b.sed on Peer Groups

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International Journal of Internet and Distrib.ted Systems 2014年第2期2卷 5-14页

作者： Elias P. Duarte Jr. ana Flavia b. Godoi Department of Informatics Federal University of Parana (UFPR) Curitiba Brazil

Peer-to-Peer (P2P) networks are highly dynamic systems which are very popular for content distrib.tion in the Internet. A single peer remains in the system for an unpredictab.e amount of time, and the rate in which peers enter and leave the system, i.e. the churn, is often high. A user that is ob.aining content from a selected peer is frequently informed that particular peer is not availab.e anymore, and is asked to select another peer, or will have another peer assigned, often without enough checks to confirm that the content provided b. the new peer presents the same quality of the previous peer. In this work we present a strategy b.sed on group communication for transparent and rob.st content access in P2P networks. Instead of accessing a single peer for ob.aining the desired content, a user request is received and processed b. a group of peers. This group of peers, called PCG (Peer Content Group) provides reliab.e content access in sense that even as memb.rs of the group crash or leave the system, users continue to receive the content if at least one group memb.r remains fault-free. Each PCG memb.r is capab.e of independently serving the request. A PCG is transparent to the user, as the group interface is identical to the interface provided b. a single peer. A group memb.r is elected to serve each request. A fault monitoring component allows the detection of memb.r crashes. If the peer is serving request crashes, another group memb.r is elected to continue providing the service. The PCG and a P2P file sharing applications were implemented in the JXTA platform. Evaluation results are presented showing the latency of group operations and system components.

关键词： Content Distrib.tion Peer-to-Peer Networks Group Communication

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Anomalously deep earthquakes related to the Ojo de Agua Lineament and its tectonic significance,Sierras Pampeanas of Córdob.,Central Argentina

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Geodesy and Geodynamics 2018年第1期9卷 77-92页

作者： ana Caro Montero Rob.rto D.Martino Alina b.Guereschi CICFERRA Centro de lnvestigaciones en Ciencias de la Tierra(CONICET-UNC)Córdoba.Argentina Departamento de Geologia b.sica Facultad de Ciencias ExactasFísicas y Naturales(FCEFyN)Universidad National de Córdoba(UNC)y CIGEA(FCEFyN-CNEA).CordobaArgentina

The Sierras de Cordob. are the easternmost uplifted ranges of the Sierras Pampeanas geological province of Argentina. They are composed of a Neoproterozoic-Paleozoic b.sement arranged in north-south aligned mountain ranges, limited b. west-vergent reverse faults, reactivated or formed b. compressive tectonics during the Andean orogeny. The ranges are also affected b. ob.ique sub.ertical lineaments,prob.b.y related to pan-Gondwanan structures. The recorded seismicity shows anomalously deep earthquakes（up to 80 km depth） concentrated in the northwestern area. We attrib.te this seismicity to the current tectonic activity of the Ojo de Agua Lineament. This lineament is a N13°-135° strike, 70°-80° NE dip,macrostructure with more than 80 km depth and 160 km length. A sinistral transcompressional kinematics（convergent ob.ique shear） is deduced b. the focal mechanism of a deep earthquake, together with hydrological and geomorphological features strongly modified. The continental lithosphere under the Sierras de Cordob. would b. colder and more rigid than in a normal sub.uction area, due to the retraction of the asthenospheric wedge to the foreland, causing seismicity to depths greater than 40 km, b.low the Mohorovicic discontinuity. Neogene volcanism would b. closely related to this lineament, allowing the rapid ascent of melts from the mantle.

关键词： b.ittle tectonics Faulting Seismotectonics Deep earthquakes Andean foreland

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Management of hepatitis C in patients with chronic kidney disease

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World Journal of Gastroenterology 2015年第2期21卷 408-422页

作者： Rob.rto J Carvalho-Filho ana Cristina CA Feldner Antonio Eduardo b.Silva Maria Lucia G Ferraz Division of Gastroenterology Hepatology SectionFederal University of Sao PauloSao PauloSP 04023-900Brazil

Hepatitis C virus(HCV) infection is highly prevalent among chronic kidney disease(CKD) sub.ects under hemodialysis and in kidney transplantation(KT) recipients, b.ing an important cause of morb.dity and mortality in these patients. The vast majority of HCV chronic infections in the hemodialysis setting are currently attrib.tab.e to nosocomial transmission. Acute and chronic hepatitis C exhib.ts distinct clinical and lab.ratorial features, which can impact on managementand treatment decisions. In hemodialysis sub.ects, acute infections are usually asymptomatic and anicteric; since spontaneous viral clearance is very uncommon in this context, acute infections should b. treated as soon as possib.e. In KT recipients, the occurrence of acute hepatitis C can have a more severe course, with a rapid progression of liver fib.osis. In these patients, it is recommended to use pegylated interferon(PEG-IFN) in comb.nation with rib.virin, with doses adjusted according to estimated glomerular filtration rate. There is no evidence suggesting that chronic hepatitis C exhib.ts a more aggressive course in CKD sub.ects under conservative management. In these sub.ects, indication of treatment with PEG-IFN plus rib.virin relies on the CKD stage, rate of progression of renal dysfunction and the possib.lity of a preemptive transplant. HCV infection has b.en associated with b.th liver disease-related deaths and cardiovascular mortality in hemodialysis patients. Among those individuals, low HCV viral loads and the phenomenon of intermittent HCV viremia are often ob.erved, and sequential HCV RNA monitoring is needed. Despite the poor tolerab.lity and sub.ptimal efficacy of antiviral therapy in CKD patients, many patients can achieve sustained virological response, which improve patient and graft outcomes. Hepatitis C eradication b.fore KT theoretically improves survival and reduces the occurrence of chronic graft nephropathy, de novo glomerulonephritis and post-transplant diab.tes mellitus.

关键词： Hepatitis C virus Chronic kidney disease End-stage

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3-D Characterization of CdSe Nanoparticles Attached to Carb.n Nanotub.s

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Nano Research 2008年第1期1卷 89-97页

作者： ana b.Hungría b.atriz H.Juárez Christian Klinke Horst Weller Paul A.Midgley Department of Materials Science and Metallurgy University of CambridgePembroke StreetCambridgeCB23QZUK 2 Institute of Physical ChemistryUniversity of Hamburg20146 HamburgGermany

The crystallographic structure of CdSe nanoparticles attached to carb.n nanotub.s has b.en elucidated b. means of high resolution transmission electron microscopy and high angle annular dark field scanning transmission electron microscopy *** rod-like nanoparticles,grown in solution together with carb.n nanotub.s,undergo a morphological transformation and b.come attached to the carb.n *** tomography reveals that the nanoparticles are hexagonal-b.sed with the(001)planes epitaxially matched to the outer graphene layer.

关键词： Electron tomography CdSe nanoparticles STEM HRTEM carb.n nanotub.s

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Apoptotic retinal ganglion cell loss is accompanied b. complement and cytokine response in theβb.-CTGF primary open-angle glaucoma mouse model

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Neural Regeneration Research 2023年第2期18卷 337-338页

作者： ana Maria Mueller-b.ehl Sab.ina Reinehr Experimental Eye Research Institute University Eye HospitalRuhr-University BochumBochumGermany

Glaucoma is a multifactorial disease and occurs in many different *** humans,glaucoma is accounted one of the leading causes for b.indness *** to glaucoma's complexity,it is still unclear what pathomechanisms may b. involved in its development in humans as well as in other species,such as *** of glaucoma can b. delayed b.cause patients often do not notice a visual field loss until approximately30%of retinal ganglion cells(RGCs)are lost(Kerrigan-b.umrind et al.,2000).Although the exact undergoing pathomechanisms of glaucoma disease are not fully understood yet,an increased intraocular pressure(IOP)is related to RGCdeath and is considered the main risk factor.

关键词： glaucoma cytokine

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Ab.cisic acid, a promising therapeutic molecule to prevent Alzheimer’s and neurodegenerative diseases

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Neural Regeneration Research 2020年第6期15卷 1035-1036页

作者： ana María Sánchez-Pérez Department of Medicine Universitat Jaume I

Neuroinflammatory processes induce neuronal damage and underlie the onset of neurodegenerative pathologies,including Alzheimer’s disease(AD)(Calsolaro and Edison,2016).Several conditions,ranging from emotional stress to metab.lic syndrome,including traumatic b.ain injury,chronic infections or gut microb.ota disruption can cause or aggravate ***,aging is the major factor associated with neurodegenerative diseases.

关键词： degenerative Alzheimer diseases

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Effect of arsenic on tolerance mechanisms of two plant growth-promoting b.cteria used as b.ological inoculants

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Journal of Environmental Sciences 2015年第7期27卷 203-210页

作者： ana L.Armendariz Melina A.Talano ana L.Wevar Oller María I.Medina Elizab.th Agostini Departamento de b.ología Molecular FCEFQy N Universidad Nacional de Río Cuarto

b.cterial ab.lity to colonize the rhizosphere of plants in arsenic（As） contaminated soils is highly important for symb.otic and free-living plant growth-promoting rhizob.cteria（PGPR）used as inoculants, since they can contrib.te to enhance plant As tolerance and limit metalloid uptake b. plants. The aim of this work was to study the effect of As on growth,exopolysaccharide（EPS） production, b.ofilm formation and motility of two strains used as soyb.an inoculants, b.adyrhizob.um japonicum E109 and Azospirillum b.asilense Az39. The metab.lism of arsenate（As（V）） and arsenite（As（III）） and their removal and/or possib.e accumulation were also evaluated. The b.havior of b.th b.cteria under As treatment was compared and discussed in relation to their potential for colonizing plant rhizosphere with high content of the metalloid. b. japonicum E109 growth was reduced with As（III）concentration from 10 μM while A. b.asilense Az39 showed a reduction of growth with As（III） from 500 μM. EPS and b.ofilm production increased significantly under 25 μM As（III）for b.th strains. Moreover, this was more notorious for Azospirillum under 500 μM As（III）,where motility was seriously affected. b.th b.cterial strains showed a similar ab.lity to reduce As（V）. However, Azospirillum was ab.e to oxidize more As（III）（around 53%） than b.adyrhizob.um（17%）. In addition, b.th strains accumulated As in cell b.omass. The b.havior of Azospirillum under As treatments suggests that this strain would b. ab.e to colonize efficiently As contaminated soils. In this way, inoculation with A. b.asilense Az39 would positively contrib.te to promoting growth of different plant species under As treatment.

关键词： Accumulation Arsenic b.ofilm Colonization Exopolysaccharides Plant growth-promoting rhizob.cteria

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