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Feeding behavior, life history, and virus transmission ability of Bemisia tabaci Mediterranean species (Hemiptera: Aleyrodidae) under elevated CO2

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Insect Science 2020年第3期27卷 558-570页

作者： Ainara Penalver-Cruz Elisa Garzo Ines Prieto-ruiz Miguel Diaz-Carro ana Winters Aranzazu Moreno Alberto Fereres Departamento de Proteccion vegetal Instituto de Ciencias Agrarias(ICA-CSIC)MadridSpain Laboratorio de Control Biologico Instituto de Ciencias BiologicasUniversidad de TalcaTalcaChile Institute of Biological Environmental and Rural SciencesAberystwyth UniversityAberystwythCeredigionUK

The continuous rise of CO2 concentrations in the atmosphere is reducing plant nutritional quality for herbivores and indirectly affects their *** whitefly(Bemisia tabaci,Gennadius)is a major worldwide pest of agricultural crops causing significant yield *** study investigated the plant‐mediated indirect effects of elevated CO2 on the feeding behavior and life history of *** Mediterranean *** were grown under elevated and ambient CO2 concentrations for 3 weeks after which plants were either used to monitor the feeding behavior of whiteflies using the Electrical Penetration Graph technique or to examine fecundity and fertility of *** leaf carbon,nitrogen,phenols and protein contents were also analyzed for each *** tabaci feeding on plants exposed to elevated CO2 showed a longer phloem ingestion and greater fertility compared to those exposed to ambient CO2 suggesting that *** is capable of compensating for the plant nutritional ***,this study looked at the transmission of the virus Tomato yellow leaf curl virus(Begomovirus)by *** exposing source and receptor tomato plants to ambient or elevated CO2 levels before or after virus transmission *** indicate that *** transmitted the virus at the same rate independent of the CO2 levels and plant ***,we conclude that *** Mediterranean species prevails over the difficulties that changes in CO2 concentrations may cause and it is predicted that under future climate change conditions,*** would continue to be considered a serious threat for agriculture worldwide.

关键词： carbon dioxide eggplant EPG fitness tomato whiteflies

Purification,characterization and antiproliferative activity of L-asparaginase from Aspergillus oryzae CCT 3940 with no glutaminase activity

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Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 785-794页

作者： Fernanda Furlan Goncalves Dias ana Lucia Tasca Gois ruiz Adriana Della Torre Helia Harumi Sato Department of Food Science School of Food EngineeringUniversity of Campinas Division of Pharmacology and Toxicology Multidisciplinary Center for ChemicalBiological and Agricultural ResearchUniversity of Campinas

Objective:To explore the anti-proliferative activity of purified L-asparaginase from Aspergillus oryzae CCT 3940(***).Methods:L-asparaginase was produced by submerged fermentation and purified to electrophoresis homogeneity by ionic exchanged chromatography in a fast protein liquid chromatographic *** purified enzyme was characterized and used for the antiproliferative assay against nine tumor cell lines and one non-tumor cell ***:The free glutaminase L-asparaginase was purified 28.6 fold.L-asparaginase showed high stability under physiological condition,remaining stable in the p H range7.0–8.0 after 1 h incubation at temperature range 30–45℃.The Km and Vmax values of purified L-asparaginase were estimated as 0.66 mmol/L and 313 IU/mL,*** purified enzyme could inhibit the growth of a broad range of human tumor cell lines at the concentrations ***,the enzyme from *** CCT 3940 could inhibit tumor growth of leukemia cell line(K562) with a total growth inhibition value of(3.2 ± 2.5) IU/mL and did not inhibit the non-carcinogenic human cell line growth at the concentrations ***:The sensitivity of the cells lines to purified L-asparaginase from *** CCT 3940 appeared to be concentration dependent affording a more significant decrease in cell growth than that observed for the commercial L-asparaginase from Escherichia *** L-asparaginase from *** CCT 3940 has a high potential for pharmaceutical exploitation in the treatment of leukemia.

关键词： L-asparaginase Aspergillus oryzae Purification Anti-tumoral activity

Mixed iridium-nickel oxides supported on antimony-doped tin oxide as highly efficient and stable acidic oxygen evolution catalysts

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Materials Futures 2024年第1期3卷 165-180页

作者： Jonathan ruiz Esquius Alec P LaGrow Haiyan Jin Zhipeng Yu ana Araujo Rita Marques Adélio Mendes Lifeng Liu Clean Energy Cluster International Iberian Nanotechnology LaboratoryAv.Mestre JoséVeigaBraga 4715-330Portugal Carbon Science and Technology Institute INCAR-CSICFrancisco Pintado Fe 26Oviedo 33011Spain Scientific Imaging Section Okinawa Institute of Science and Technology Graduate UniversityKunigami-gunOkinawa 904-0412Japan Songshan Lake Materials Laboratory Dongguan 523808People’s Republic of China Laboratory for Process Engineering EnvironmentBiotechnology and EnergyFaculty of EngineeringUniversity of PortoRua Dr Roberto FriasPorto 4200-465Portugal Associate Laboratory in Chemical Engineering Faculty of EngineeringUniversity of PortoRua Dr Roberto FriasPorto 4200-465Portugal.

Proton exchange membrane(PEM)water electrolysis represents a promising technology for green hydrogen production,but its widespread deployment is greatly hindered by the indispensable usage of platinum group metal catalysts,especially iridium(Ir)based materials for the energy-demanding oxygen evolution reaction(OER).Herein,we report a new sequential precipitation approach to the synthesis of mixed Ir-nickel(Ni)oxy-hydroxide supported on antimony-doped tin oxide(ATO)nanoparticles(IrNiyO_(x)/ATO,20 wt.%(Ir+Ni),y=0,1,2,and 3),aiming to reduce the utilisation of scarce and precious Ir while maintaining its good acidic OER *** tested in strongly acidic electrolyte(0.1 M HClO_(4)),the optimised IrNi1Ox/ATO shows a mass activity of 1.0 mAµgIr^(−1) and a large turnover frequency of 123 s^(−1) at an overpotential of 350 mV,as well as a comparatively small Tafel slope of 50 mV dec^(−1),better than the IrOx/ATO control,particularly with a markedly reduced Ir loading of only 19.7µgIr cm^(−2).Importantly,IrNi1O_(x)/ATO also exhibits substantially better catalytic stability than other reference catalysts,able to continuously catalyse acidic OER at 10 mA cm^(−2) for 15 h without obvious *** in-situ synchrotron-based x-ray absorption spectroscopy confirmed that the Ir^(3+)/Ir^(4+)species are the active sites for the acidic ***,the performance of IrNi1Ox/ATO was also preliminarily evaluated in a membrane electrode assembly,which shows better activity and stability than other reference *** IrNi1Ox/ATO reported in this work is a promising alternative to commercial IrO_(2) based catalysts for PEM electrolysis.

关键词： mixed oxides oxygen evolution reaction polymer electrolyte membrane antimony doped tin oxide membrane electrode assembly

Natural variability of essential oil and antioxidants in the medicinal plant Turnera diffusa

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Asian Pacific Journal of Tropical Medicine 2017年第2期10卷 114-119页

作者： ana Lucía Urbizu-González Octelina Castillo-ruiz Guillermo Cristian Guadalupe Martínez-Ávila Jorge Ariel Torres-Castillo Universidad Autónoma de Tamaulipas Instituto de Ecología AplicadaDivisión del Golfo 356Col.Libertad87019Ciudad VictoriaTamaulipasMéxico Universidad Autónoma de Tamaulipas Unidad Académica Multidisciplinaria Reynosa AztlánCalle 16 y Lago de Chápala s/n Col.AztlánC.P.88740ReynosaTamaulipasMéxico Universidad Autónoma de Nuevo León Facultad de AgronomíaFrancisco Villa s/n.Col.Ex Hacienda El Canadá66050General EscobedoNuevo LeónMéxico

Objective:To evaluate differences in yield and composition of the essential oil and antioxidant contents in *** plants from localities in central region of ***:Samples were collected in Tamaulipas,Mexico in the arid *** oil was obtained through steam distillation and analyzed using *** contents,antioxidant activities using ABTS and ferric reducing antioxidant power(FRAP) methods also were ***:A total of 21 compunds were identified in the essential oils;nevertheless,only Eucalyptol,1,4-Methanocycloocta[d]pyridazine,1,4,4a,5,6,9,10,10a-octahydro-11,11-dimethyl-,(1à,4à,4aà,10aà) y Ethanone,1-(1,3-dimethyl-3-cyclohexen-1-yl) were detected in the three *** contents were registered in the sample from Padrón y Juárez with phenolic content of 33.85 mg GAE/g of dry material and antioxidant activities with ABTS 72.32% and with FRAP 21.33 mg GAE/g of dry *** differences were observed in essential oil,phenolics and antioxidants contents between ***:Results suggest that climatic differences and origin influence the phytochemicals in the medicinal plant ***,and thus,it is worth to consider such effects for industrial and medicinal purposes.

关键词： Essential oil Antioxidants Phytochemicals Chemical variation

Resilience to structural and molecular changes in excitatory synapses in the hippocampus contributes to cognitive function recovery in Tg2576 mice

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Neural Regeneration Research 2024年第9期19卷 2068-2074页

作者： Carolina Aguado Sara Badesso JoséMartínez-Hernández Alejandro Martín-Belmonte Rocío Alfaro-ruiz Miriam Fernández ana Esther Moreno-Martínez Mar Cuadrado-Tejedor ana García-Osta Rafael Luján Synaptic Structure Laboratory Instituto de Investigación en Discapacidades Neurológicas(IDINE)Department of Medical SciencesFacultad de MedicinaUniversidad de Castilla-La ManchaCampus BiosanitarioAlbaceteSpain Gene Therapy for Neurological Disease Program Center for Applied Medical Research(CIMA)University of NavarraInstituto de Investigación Sanitaria de Navarra(IdiSNA)PamplonaSpain Pharmacology Unit Department of Pathology and Experimental TherapeuticsFaculty of Medicine and Health SciencesInstitute of NeurosciencesUniversity of BarcelonaBarcelonaSpain Neuropharmacology and Pain Group Neuroscience ProgramInstitut d’InvestigacióBiomèdica de BellvitgeIDIBELLL’Hospitalet de LlobregatSpain Department of Pathology Anatomy and PhysiologySchool of MedicineUniversity of NavarraPamplonaSpain UniversitéGrenoble Alpes Grenoble Institut des NeurosciencesGrenobleFrance

Plaques of amyloid-β(Aβ)and neurofibrillary tangles are the main pathological characteristics of Alzheimer’s disease(AD).However,some older adult people with AD pathological hallmarks can retain cognitive *** the factors that lead to this cognitive resilience to AD offers promising prospects for identifying new therapeutic *** hypothesis focuses on the contribution of resilience to changes in excitatory synapses at the structural and molecular levels,which may underlie healthy cognitive performance in aged AD *** the Morris Water Maze test,we selected resilient(asymptomatic)and cognitively impaired aged Tg2576 *** the enzyme-linked immunosorbent assay showed similar levels of Aβ42 in both experimental groups,western blot analysis revealed differences in tau pathology in the pre-synaptic supernatant *** further investigate the density of synapses in the hippocampus of 16-18 month-old Tg2576 mice,we employed stereological and electron microscopic *** findings indicated a decrease in the density of excitatory synapses in the stratum radiatum of the hippocampal CA1 in cognitively impaired Tg2576 mice compared with age-matched resilient Tg2576 and non-transgenic ***,through quantitative immunoelectron microscopy in the hippocampus of impaired and resilient Tg2576 transgenic AD mice,we uncovered differences in the subcellular localization of glutamate ***,the density of GluA1,GluA2/3,and mGlu5 in spines and dendritic shafts of CA1 pyramidal cells in impaired Tg2576 mice was significantly reduced compared with age-matched resilient Tg2576 and non-transgenic ***,the density of GluA2/3 in resilient Tg2576 mice was significantly increased in spines but not in dendritic shafts compared with impaired Tg2576 and non-transgenic *** subcellular findings strongly support the hypothesis that dendritic spine plasticity and synaptic machinery in the hippocampus play cr

关键词： aging Alzheimer´s disease cognitive hippocampus immunoelectron microscopy resilience synapse

Mitomycin C in pterygium treatment

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International Journal of Ophthalmology(English edition) 2016年第3期9卷 465-468页

作者： Thiago Goncalves dos Santos Martins ana Luiza Fontes de A zevedo Costa Milton ruiz Alves Roger Chammas Paulo Schor Federal University of Sao Paulo (UNIFESP) Sao Paulo 04023062 Brazil University of Sao Paulo (USP) Sao Paulo 01246903 Brazil

Pterygium is a benign lesion usually growing from the nasal side of the conjunctiva onto the *** cases of pterygium does not cause problem or requires specific *** exact cause of pterygium is not clear yet,but some factors are pointed as causes,being the most important the long-term ultraviolet ray *** surgery is usually considered when there are symptoms that do not respond to conservative *** is the main complication of the surgery,and much has been done to avoid *** C（MMC） has been used as a fibroblast proliferation inhibitor during the surgery to reduce the chance of recurrence of the *** review describes the use of MMC as an adjunctive,the optimal dosage,the duration of administration of MMC and possible complications,when used during,after and before the *** studies suggest that increased exposure（dose or duration） of MMC is associated with a lower recurrence,but with higher risks of complications.

关键词： mitomycin C pterygium surgery recurrence rate

Coping with drought?Effects of extended drought conditions on soil invertebrate prey and diet selection by a fossorial amphisbaenian reptile

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Current Zoology 2023年第4期69卷 367-376页

作者： José Martín Jesús Ortega Roberto García-Roa Gonzalo Rodríguez-ruiz ana Pérez-Cembranos Valentín Pérez-Mellado Departamento de Ecología Evolutiva Museo Nacional de Ciencias NaturalesCSICMadridSpain Institute of Evolutionary Biology CSIC-Universitat Pompeu FabraBarcelonaSpain Ethology Lab Cavanilles Institute of Biodiversity and Evolutionary BiologyUniversity of ValenciaValenciaSpain Departamento de Biología Animal Universidad de SalamancaSalamancaSpain

Arid climates are characterized by a summer drought period to which animals seem ***,in some years,the drought can extend for unusually longer *** the effects of these current extreme weather events on biodiversity can help to understand the effects of climate change,as models predict an increase in drought ***,we examined the effects of“unusual”extended drought on soil invertebrate prey availability and on diet composition(based on fecal contents)and diet selection of a fossorial amphisbaenian,the checkerboard worm lizard Trogonophis *** data show interannual variations in summer drought *** abundance and diversity of soil invertebrates in spring were high,and similar to those found in a“normal”early autumn,after some rain had ended with the summer *** contrast,in years with“unusual”extended drought,abundance,and diversity of soil invertebrates in early autumn were very ***,there were seasonal changes in amphisbaenians’diet;in autumn with drought,prey diversity,and niche breadth decreased with respect to spring and autumns after some rain had *** did not eat prey at random in any season,but made some changes in prey selection that may result from drought-related restrictions in prey ***,in spite that amphisbaenians showed some feeding flexibility,their body condition was lower in autumn than in spring,and much lower in autumn with *** extended drought became the norm in the future,amphisbaenians might suffer important negative effects for their health state.

关键词： amphisbaenians drought feeding ecology soil invertebrates Trogonophis wiegmanni weather conditions

Historical fluxes of metal and metalloids in an aquatic ecosystem affected by land-use change and mining activities in northwestern Mexico

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International Journal of Sediment Research 2023年第5期38卷 724-738页

作者： Ochoa-Contreras Roberto Jara-Marini Martín Enrique ruiz-Fernández ana Carolina Sanchez-Cabeza Joan Albert Meza-Figueroa Diana Pérez-Bernal Libia Hascibe Posgrado en Ciencias Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo 2. Centro de Investigación en Alimentación y Desarrollo Unidad Hermosillo Unidad Acad emica Mazatlán Instituto de Ciencias del Mar y Limnología Universidad Nacional Autónoma de M exico Departamento de Geología División de Ciencias Exactas y Naturales Universidad de Sonora Rosales y Encinas

Freshwater reservoirs are essential owing because of their ecological, economic, and social *** are particularly vulnerable to contamination, as of metal and metalloids, derived from anthropogenic activities like mining. The temporal variations in trace element concentrations(arsenic(As),mercury(Hg), chromium(Cr), copper(Cu), nickel(Ni), lead(Pb), and zinc(Zn)), enrichment, fluxes, and possible sources were evaluated by studying two sediment cores from the La Angostura(ANG) Reservoir(northwest Mexico), using210Pb geochronology. The enrichment factors showed from null to minor enrichment for most elements, but moderate to severe enrichment of mercury(Hg). Most trace element concentrations had a detrital origin, and notable Hg concentration increases since the past decade were associated with severe drought periods, likely resulting from wildfires. The observed sediment concentrations of As and Hg can cause adverse effects on biota in the ecosystem since they are above the probable effect level(PEL). Development of strategies for metal attenuation in this reservoir is recommended and metals should be controlled until specific ecotoxicological studies are performed.

关键词： Aquatic ecosystems ~(210)Pb geochronology Mining activities Metals Metalloids Enrichment factors

The Convex-Set Algebra and Intersection Theory on the Toric Riemann–Zariski Space

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Acta Mathematica Sinica,English Series 2022年第3期38卷 465-486页

作者： ana María BOTERO Institut Für Mathematik Universität RegensburgUniversitätsstr.3193053RegensbergGermany

In previous work of the author,a top intersection product of toric b-divisors on a smooth complete toric variety is *** is shown that a nef toric b-divisor corresponds to a convex set and that its top inetersection number equals the volume of this convex *** goal of this article is to extend this result and define an intersection product of sufficiently positive toric b-classes of arbitrary *** this,we extend the polytope algebra of McMullen to the so called convex-set algebra and we show that it embeds in the toric b-Chow *** this way,the convex-set algebra can be viewed as a ring for an intersection theory for sufficiently positive toric *** an application,we show that some Hodge type inequalities are satisfied for the convex set algebra.

关键词： Convex geometry intersection theory toric varieties b-Chow groups Riemann–Zariski spaces