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Partial purification,immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus

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Advances in Enzyme Research 2013年第4期1卷 79-90页

作者： ana lucia Ferrarezi Daniele H.Pivetta Gustavo Orlando Bonilla-Rodriguez Roberto da Silva Jose Manuel Guisan Eleni Gomes Benevides Costa Pessela Department of Biology Laboratory of Applied Biochemistry and MicrobiologyIBILCE-UNESPSao Jose do Rio Preto SPBrazil Department of Chemistry and Environmental Sciences IBILCE-UNESPSao Jose do Rio Preto SPBrazil Department of Enzyme Biocatalysis Institute of Catalysis and PetrochemistryCSICCampus UAMCantoblancoMadridSpain Department of Biotechnology and Food Microbiology Research Institute for Food ScienceCIAL-CSICCalleNicolás Cabrera 9Campus UAMMadridSpain

Lipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated by solid state fermentation producing lipolytic activity of about 0.5 U/mL(4U/g). A partial purification by gel filtration chromatography in Se-phacryl S-100 allowed obtaining a yield of about 85% and a purification factor of 5.7. Our results revealed that the purified enzyme is very stable with some significant differences in its properties when compared to crude extract. The crude enzyme extract has an optimum pH and temperature of 7.5 ° C and 40 ° C, respectively. After purification, a shift of the optimum pH from 7 to 8 was observed, as well as a rise in optimumtemperature to 60 ° C and an increase in stability. The enzyme was immobilized on CNBr-Agarose and Octyl-Agarose supports, having the highest immobilization yield of 94% in the second resin. The major advantage of immobilization in hydrophobic media such as Octyl is in its hyper activation, which in this case was over 200%, a very interesting finding. Another advantage of this type of immobilization is the possibility of using the derivatives in biotechnological applications, such as in oil enriched with omega-3 as the results obtained in this study display the hydrolysis of 40% EPA and 7% DHA from sardine oil, promising results compared to the literature.

关键词： Enzyme Immobilization Solid State Fermentation Purification n-3 Polyunsatured Fatty Acids Rhizomucor pusillus

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<i>Angiostrongylus costaricensis</i>: Systematic Review of Case Reports

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Advances in Infectious Diseases 2014年第1期4卷 36-41页

作者： Angela Romero-Alegría Moncef Belhassen-García Virginia Velasco-Tirado ana Garcia-Mingo lucia Alvela-Suárez Javier Pardo-Lledias Miguel Cordero Sánchez Department of Internal Medicine CAUSA IBSAL Salamanca Spain Department of Internal Medicine CAUSA IBSAL Salamanca Spain CIETUS University of Salamanca Salamanca Spain Department of Internal Medicine General Hospital of Palencia “Río Carrión” Palencia Spain

Introduction: Abdominal angiostrongyliasis is an infrequently diagnosed disease because it is little known and is usually well tolerated. Methods: We carried out a systematic review with PubMed as a search engine for the MedLine database, and we analysed the clinical, epidemiological and analytical parameters of this disease. Results: In total, reports of 27 case have been found, together with a series of 116 patients, which were later augmented up to 194 patients. In the results, we have observed a predominance of male patients, and Costa Rica, Brazil and the United States as the main countries of origin. Typical symptoms include abdominal pain on the right iliac fossa, with leukocytosis and eosinophilia. Discussion: There are some serological diagnostic methods, although lack of standardization leads to most of the diagnoses being reached via pathology. Surgery is the usual treatment approach. Although there are medical options, this is a controversial subject because of the low number of cases published in the literature, which implies an absence of solid studies.

关键词： Angiostrongylus costaricensis Abdominal Angiostrongyliasis Acute Abdomen Eosinophilia

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Characteristics of Mesenchymal Stem Cells under Hypoxia

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CellBio 2013年第1期2卷 11-19页

作者： Bruna Amorin ana Paula Alegretti Vanessa de Souza Valim Annelise Martins Pezzi da Silva Maria Aparecida Lima da Silva Felipe Sehn lucia Silla Cell Technology Center Experimental Research Center Hospital de Clínicas de Porto Alegre-Porto AlegreRio Grande do Sul Brazil

Mesenchymal stem cells (MSC) are considered non-hematopoietic multipotent stem cells with self-renewal properties and the ability to differentiate into a variety of mesenchymal tissues. Optimal conditions for the culture of these cells have been the subject of investigation for several years. In particular, ideal oxygen tension levels have not been established in the literature. In physiological environments, oxygen tension may vary from 12% in peripheral blood to 1% in the deep zone of cartilage regions. In any case, oxygen tension is considerably lower in vivo when compared with the normal atmosphere of standard cell culture conditions (21%). The objective of this study was to review the literature available on MSC characteristics (cell cycle, survival, proliferation, differentiation, morphology, immunophenotype, cytogenetics) when cultured under hypoxic conditions. Our focus on optimal culture conditions is justified by the key role currently played by these cells in regenerative medicine.

关键词： Mesenchymal Stem Cells Culture Hypoxia

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Information and Knowledge Flows in the Network of the Education through Work for Family Health Program

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Social Networking 2014年第2期3卷 119-126页

作者： ana Aurea Alécio de Oliveira Rodrigues Claudia Ribeiro Santos lopes Eliane Santos Souza Inacio de Sousa Fadigas Hernane Borges de Barros Pereira Universidade Estadual de Feira de Santana Feira de SantanaBrazil Programa de Difusao do Conhecimento DMMDC/UFBASalvadorBrazil Universidade Estadual do Sudoeste da Bahia JequiéBrazil Universidade Federal da Bahia SalvadorBrazil Universidade do Estado da Bahia SalvadorBrazil Programa de Modelagem Computacional SENAI CimatecSalvadorBrazil

The Education through Work for Family Health Program (PET Saúde, in Portuguese) created by the Ministries of Education and Health is a proposal to integrate service, learning and community. The goal of PET Saúde is to foster the formation of learning groups within the Family Health Strategy scope. While being studied at the city of Feira de Santana, the program was structured with one coordinator and eleven tutorial groups, distributed in 27 family health offices, which constituted a social network where the construction and knowledge diffusion occur. This article proposes the analysis of the information and knowledge flows of this network through the social networks analysis. This study highlights the play of PET Saúde’s coordinator in the processes of organization and articulation as central vertex of the network, and some scholars in the articulation of different tutorial groups. The results show a fragmentation in the PET Saúde network when the coordinator vertex is removed, whereas 5 components are generated where 4 tutorial groups appear isolated.

关键词： Social Networks Education-Service Interaction Health Information Knowledge

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Determination of Paraquat in Several Commercially Available Types of Rice

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Food and Nutrition Sciences 2018年第12期9卷 1368-1375页

作者： Thais lopes Lima Maria Aparecida Nicoletti Camila Munhoz Gabriel Ramos De Abreu Julia Zaccarelli Magalhaes Esther lopes Ricci Ricci Paula A.Faria Waziry Júlia Nathalia Alves da Costa ana Carolina Nascimento Antonio AndréRinaldi Fukushima Universidade Sao Judas Tadeu-R.Taquari Sao Paulo/SPBrazil Departamento de Farmacia.Faculdade de Ciencias Farmaceuticas.Universidade de Sao Paulo Sao Paulo/SPBrazil Instituto de Ciencias da Saude Universidade Presbiteriana MackenzieRua da ConsolacaoSao Paulo/SPBrazil Departamento de Patologia Faculdade de Medicina Veterinaria e ZootecniaUniversidade de Sao PauloSao Paulo/SPBrazil New Southeastern University Fort LauderdaleFloridaUnited States of America Veterans Affairs Medical CenterMiamiFloridaUSA Faculdade Sao Bento-Largo de Sao Bento s/no-CentroSao Paulo-SPSao Paulo/SPBrazil

Introduction: Paraquat (1,1’-dimethyl-4,4’-bipyridine-dichloride) is a herbicide used to combat weeds and improve crop productivity. Its acute toxicity can cause fatal poisoning in humans and animals and several studies have shown a strong correlation between paraquat and Parkinson’s disease. Objective: To determine the concentration of paraquat in different types of rice in Brazil. Method: Paraquat quantification is based on a complexation reaction with sodium dithionite, which generates a blueish color compound, whose absorbance was read in a spectrophotometer at the 600 nm wavelength. Results: Five samples were found to be above the maximum allowed limit, while one sample had a concentration in the upper allowed limit. The method employed did not allow quantification of black rice. Conclusion: There is a need for identification of the type of pesticide used in each examined crop as well as their respective dates of remission in order to improve the safety of agricultural practices.

关键词： Paraquat Herbicide Rice

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在量子通信中遏制木马攻击

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物理 2015年第11期44卷 769-769页

作者：戴闻 ana lopes 中国科学院理化技术研究所

就像古代神话＂特洛伊木所描述的,木马攻击是一种策它秘密潜入到受安全保护的空按照光学量子密钥分发（QKD）协的说明,这种攻击涉及＂木马子＂潜入到光学量子密钥分发统,企图获取密钥。

关键词：木马攻击量子密钥分发量子通信特洛伊古代神话外部供电量子信息被动防御防御系统光脉冲

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Determining the Soil Erodibility for an Experimental Basin in the Semi-Arid Region Using Geoprocessing

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American Journal of Plant Sciences 2017年第12期8卷 3174-3188页

作者： Erich Celestino Braga Pereira Fernando Bezerra lopes Francisco Emanoel Firmino Gomes Aldenia Mendes Masceno de Almeida ana Caroline Messias de Magalhaes Eunice Maia de Andrade Department Agricultural Engineering Federal University of CearáFortalezaBrazil Department of Soil Science Federal University of CearáFortalezaBrazil

Erosion is the natural process which has the greatest environmental impact, and is the principal trigger for desertification around the globe. The main model used to estimate soil loss by erosion is the Universal Soil Loss Equation (USLE), which unites the major factors that influence erosion into one equation. The soil erodibility factor (K) is the component of this equation that represents soil physics, and is defined as the inherent capacity of the soil to withstand disintegration of its particles and their subsequent transport. The use of geostatistics is seen as an alternative in spatializing this variable from sampled to non-sampled points. The aim of this study therefore, was to determine the soil erodibility factor for an experimental basin in the semi-arid region of Brazil, in addition to generating the soil erodibility map using geostatistics. Disturbed and undisturbed soil samples were collected from 35 points, and laboratory samples were processed to determine the granulometry, permeability and organic matter of the soil, data which are used to determine the K-factor. Kriging was performed to spatialize the study variable, when spherical, exponential and Gaussian semivariograms were tested for generation of the soil erodibility map, these being evaluated by their respective deviations resulting from cross-validation. The mean value of K for the Haplic Luvisol was 0.0328 ton·ha·h/ha·MJ·mm;for the eutrophic Red-Yellow Argisol it was 0.0258 ton·ha·h/ha·MJ·mm;and for the Fluvic Neosol, it was 0.0424 ton·ha·h/ha·MJ·mm. The experimental basin is classified as highly erodible. The semivariogram that presented the best fit for generating the soil erodibility map of the study area was Gaussian.

关键词： Erosion Universal Soil Loss Equation Geostatistics

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Total Occlusion of Anterior Descending Artery （AD） without ST-Segment Elevation： DE Winter Pattern

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Journal of Pharmacy and Pharmacology 2018年第6期6卷 595-599页

作者： ana Carolina Brito de Alcantara Francisco Marcelo Sobreira Filho Danielli Oliveira da Costa Lino lucia de Sousa Belem Carlos Jose Mota de Lima Unichristus Fortaleza 60192-350 Brasil Hospital Dr. Carlos Alberto Studart Gomes Fortaleza 60846-190 Brasil

The recognition of the presence of ST-segment elevation in the context of ACS （acute coronary syndrome） is fundamental for the good outcome of the case. However, some electrocardiographic patterns have been highlighted by the fact that there is no elevation, but they represent a severity similar to ACS with ST elevation. Hence, it is necessary that doctors who do the first service recognize these standards *** electrocardiographic pattern of association between the ST-segment depression and the hyperacute T waves representing myocardial infarction in the anterior wall, localizing more specifically in the anterior descending artery, now known as the De Winter pattern, was first described by Dressler, and then described by Winter six years later. Winter observed that, out of a series of 1,452 cases, 2% of patients with anterior wall infarction had the pattern of ST-segment depression, positive and symmetrical T waves, and occasionally mild ST-segment elevation at AVR derivation. Those patients are admitted to the emergency room with a typical chest pain. However, because the electrocardiographic findings of this syndrome are not known to many health professionals, in many cases the patients do not receive adequate care and have fatal outcome, since mortality is relevant in these cases. Thus, the rapid recognition of this pattern is extremely necessary for the correct and effective intervention.

关键词： Electrocardiography infarction cardiac catheterization cardiology.

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Kaphta:Text mining web tool to extract information on the anticancer activity of polyphenols

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Journal of Polyphenols 2022年第2期4卷 87-100页

作者： Ramon Gustavo Teodoro Marques da Silva Samuel Lucas Santos Gomes Paulo Muniz deÁvila Gustavo Joséda Silva ana lucia Fachin Edilson Carlos Caritá Mozart Marins Biotechnology Unit University of Ribeirão PretoAv.Costábile Romano 2201Ribeirão PretoSP 14096-900Brazil Federal Institute of Education Science and Technology of South of Minas Gerais–IFSULDEMINASPouso AlegreMG 37550-000Brazil Medicine School University of Ribeirão PretoRibeirão PretoSP 14096-900Brazil Centro de Ciências Exatas Naturais e TecnológicasUniversidade de Ribeirão PretoRibeirão Preto SP 14096-900Brazil

In this paper,we describe the application of Kaphta architecture,a resource for text mining of the anticancer activity of *** anticancer activity of these compounds against different types of cancer has been widely reported in the literature and they are one of the most promising molecules for the development of anticancer *** architecture,which comprises four sequential and well-defined steps,uses a hybrid approach composed of a dictionary,rules and machine learning to identify abstracts containing sentences with associations between polyphenol,cancer and gene *** application of the architecture on 23826 PubMed abstracts generated a knowledge base of indexed abstracts with 172169 sentences containing,polyphenol-cancer and polyphenol-gene associations.A Web tool was implemented that allowed the user to search for information on 2006 polyphenols,240 cancers and 3121 genes entities,and 11750 polyphenol-cancer and 9160 polyphenol-gene associations indexed in the knowledge base.A ranking algorithm calculates scores for each indexed abstract considering the number and type of sentences with entities and rules recognized.A test with users demonstrated that the visualization resources on the web tool contributes to the understanding of the association between polyphenols,genes and cancers,in comparison with the PubMed *** Kaphta architecture and web tool permits to extract knowledge on the anticancer activity of polyphenols and can thus contribute to the exploration of these molecules in the development of anticancer therapies.

关键词： polyphenols cancer text-mining

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Screenning of Chlamydia trachomatis Infection among Women Attending Outpatient Clinic of Infertility

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Open Journal of Obstetrics and Gynecology 2015年第11期5卷 600-607页

作者： Heloisa lopes Lavorato Natália Prearo Moco Laura Fernandes Martin ana Gabriela Pontes Santos anaglória Pontes Marli Teresinha Cassamassimo Duarte Márcia Guimaraes da Silva Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil Department of Pathology Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil Department of Nursing Botucatu Medical School Sao Paulo State University UNESP Botucatu Brazil

Objective: The goal of this study was to determine the prevalence of C. trachomatis in women diagnosed with infertility attending the Outpatient Clinic of Infertility from Botucatu Medical School, UNESP, Brazil. Patients and Methods: This molecular study enrolled a total of 112 women. Among these patients, 62 presented primary infertility while 50 presented secondary infertility. The criteria for eligibility included women who were: reproductive-aged;no prior report of seroconversion for HIV;no antibiotic or vaginal cream used in the preceding 30 days;and abstinence from sexual intercourse for 72 hours before the visit. The women were submitted to a gynecological examination and cervical samples were collected with an endocervical cytobrush for molecular analysis of C. trachomatis. Results: The prevalence of chlamydial infection was 8% with similar prevalence between primary (8.1%) and secondary (8.0%) infertility. Conclusion: Considering the asymptomatic nature of chlamydial infection and its association with tubal factor infertility, there is a pressing need to incorporate the screening of C. trachomatis infection as part of the routine investigation for infertility. The early diagnostic by screening can minimize complications and reduce Public Health costs with Assisted Reproductive Technology.

关键词： Chlamydial Infection Infertility Molecular Diagnosis

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