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Spontaneous and Bleomycin-Induced <i>γ</i>H2AX Signals in CHO9 Metaphase Chromosomes

Advances in Bioscience and Biotechnology 2014年第7期5卷 603-616页

作者： María Vittoria Di Tomaso Silvia Basso laura Lafon-Hughes Gustavo Saona Beatriz López-Carro ana laura reyes-ábalos Pablo Liddle Environmental Quality Evaluation and Control Service Municipality of Montevideo Montevideo Uruguay Genetics Department Instituto de Investigaciones Biológicas Clemente Estable Montevideo Uruguay

In eukaryotes, a cascade of events named DNA damage response (DDR) has evolved to handle DNA lesions. DDR engages the recruitment of signaling, checkpoint control, repair and chromatin remodeling protein complexes, allowing cell cycle delay, DNA repair or induction of apoptosis. An early DDR event involves the phosphorylation of the histone variant γH2AX on serine 139 (H2AX139 phosphorylation) originating the so-called γH2AX. DDR-related H2AX139 phosphorylation have been extensively studied in interphase nuclei. More recently, γH2AX signals on mitotic chromosomes of asynchronously growing cell cultures were observed. We performed a quantitative analysis of γH2AX signals on γH2AX immunolabeled cytocentrifuged metaphase spreads, analyzing the γH2AX signal distributions of CHO9 chromosomes harboring homologous regions both in control and bleomycin (BLM)-treated cultures. We detected γH2AX signals in CHO9 chromosomes of controls which significantly increase after BLM-exposure. γH2AX signals were uniformly distributed in chromosomes of controls. However, the γH2AX signal distribution in BLM exposed cells was significantly different between chromosomes and among chromosome regions, with few signals near the centromeres and a tendency to increase towards the telomeres. Interestingly, both basal and BLM-induced γH2AX signal distribution were statistically equal between CHO9 homologous chromosome regions. Our results suggest that BLM exerts an effect on H2AX139 phosphorylation, prevailing towards acetylated and gene-rich distal chromosome segments. The comparable H2AX139 phosphorylation of homologous regions puts forward its dependence on chromatin structure or function and its independence of the position in the karyotype.

关键词： H2AX Phosphorylation on Serine 139 γH2AX Signals Metaphase Chromosomes Homologous Chromosome Regions CHO9 Chinese Hamster Cell Line

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Using music to reduce anxiety among older adults in the emergency department:a randomized pilot study

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Journal of Integrative Medicine 2017年第6期15卷 450-455页

作者： laura Belland laura Rivera-reyes Ula Hwang center for family medicine newyork-presbyterian/columbia university medical centerNew YorkNY 10032USA department of emergency medicine icahn school of medicine at mount sinaiNew YorkNY 10029USA geriatric research education and clinical centerjames j.peters vamcNew YorkNY 10029USA

BACKGROUND： An emergency department （ED） visit may be distressing and anxiety-provoking for older adults （age 〉 65 years）. No studies have specifically evaluated the effect of music listening on anxiety in older adults in the ED. OBJECTIVE： The objective of this pilot study was to evaluate the effect of music listening on anxiety levels in older ED patients. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS： This was a randomized pilot study in the geriatric ED of an urban academic tertiary medical center. This was a sample of English-speaking adults （age 〉 65 years） who were not deaf （n = 35）. Subjects consented to participate and were randomized to receive up to 60 min of music listening with routine care, while the control group received routine care with no music. Subjects in the music treatment group received headphones and an electronic tablet with pre-downloaded music, and were allowed to choose from 5 selections. MAIN OUTCOME MEASURES： The primary outcome was change in anxiety levels, measured by the state- trait anxiety inventory （STAI）, at enrollment and 1 h later. RESULTS： A total of 35 participants were enrolled： 74% were female, 40% were white, and 40% were black; of these, 32 subjects completed the study protocol. When comparing control （n = 18） against intervention subjects （n = 17）, there were no significant differences in enrollment STAI scores （43.00 ± 15.00 vs. 40.30± 12.80, P = 0.57）. STAI scores 1 hour after enrollment （after the music intervention） were significantly reduced in the intervention subjects compared to the control subjects （with reduction of 10.00± 12.29 vs. 1.88 ± 7.97, P = 0.03）. CONCLUSION： These pilot results suggest that music listening may be an effective tool for reducing anxiety among older adults in the ED.

关键词： music anxiety emergency department randomized controlled trial

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The Janus face of N-terminal lysines in α-synuclein

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Neural Regeneration Research 2020年第10期15卷 1840-1841页

作者： ana Belén Uceda laura Mari?o Miquel Adrover Institut Universitari d’Investigació en Ciències de la Salut (IUNICS) Institut d’Investigació Sanitària Illes Balears (Id ISBa) Departament de Química Universitat de les Illes Balears

Parkinson’s disease(PD)is the second most prevalent progressive neurodegenerative disorder after Alzheimer’s *** usually starts with a tremor in the extremities(usually in the hands)and gradually evolves with other symptoms such as bradykinesia,muscle stiffness,impaired posture,loss of automatic movements or speech *** symptoms worsen as the condition progresses and eventually lead to death.

关键词： impaired Alzheimer eventually

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Increased DNA binding activity of NF-κB,STAT-3,SMAD3 and AP-1 in acutely damaged liver

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World Journal of Gastroenterology 2006年第37期12卷 5995-6001页

作者： Adriana Salazar-Montes Luis Ruiz-Corro ana Sandoval-Rodriguez Alberto Lopez-reyes Juan Armendariz-Borunda Institute of Molecular Biology in Medicine and Gene Therapy CUCS University of Guadalajara Apdo. Postal 2-123 Guadalajara 44281 Jal Mexico Institute of Molecular Biology in Medicine and Gene Therapy CUCS University of Guadalajara Apdo.Postal 2-123 Guadalajara 44281 Jal Mexico OPD Hospital Civil de Guadalajara Guadalajara Jal Mexico

AIM: To investigate the role of genes and kinetics of specific transcription factors in liver regeneration, and to analyze the gene expression and the activity of some molecules crucially involved in hepatic regeneration. METHODS: USING gel-shift assay and RT-PCR, transcription factors, such as NF-κB, STAT-3, SMAD3 and AP-1, and gene expression of inducible nitric oxide synthase (iNOS), hepatocyte growth factor (HGF) and c-met were analyzed in an animal model of chemically induced hepatectomy. RESULTS: Gene expression of HGF and its receptor c-met peaked at 3 h and 24 h after acute CCl4 intoxi- cation. iNOS expression was only observed from 6 to 48 h. Transcriptional factor NF-κB had an early activation at 30 min after acute liver damage. STAT-3 peaked 3 h post- intoxication, while AP-1 displayed a peak of activation at 48 h. SMAD3 showed a high activity at all analyzed times. CONCLUSION: TNF-α and IL-6 play a central role in hepatic regeneration. These two molecules are responsible for triggering the cascade of events and switch-on of genes involved in cell proliferation, such as growth factors, kinases and cyclins which are direct participants of cell proliferation.

关键词： Hepatic regeneration Transcription factor Gene regulation

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Noninvasive investigations for non alcoholic fatty liver disease and liver fi brosis

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World Journal of Gastroenterology 2010年第38期16卷 4784-4791页

作者： Carmen Fierbinteanu-Braticevici Ion Dina ana Petrisor laura Tribus Lucian Negreanu Catalin Carstoiu Carmen Fierbinteanu-Braticevici Ana PetrisorLaura TribusLucian NegreanuCatalin CarstoiuMedical Clinic Ⅱ and Gastroenterology University Hospital Bucharest Department of Gastroenterology St John’s Hospital 7001 Bucharest Carmen Fierbinteanu-Braticevici Ana PetrisorLaura TribusLucian NegreanuCatalin CarstoiuMedical Clinic Ⅱ and GastroenterologyUniversity Hospital Bucharest

Non-alcoholic fatty liver disease (NAFLD) includes a spectrum of diseases that have insulin resistance in common and are associated with metabolic conditions such as obesity, type 2 diabetes mellitus, and dyslipidemia. NAFLD ranges from simple liver steatosis, which follows a benign course, to nonalcoholic steatohepatitis (NASH), a more severe entity, with necroinflmmation and f ibrosis, which can progress to cryptogenic cirrhosis and end-stage liver disease. Liver biopsy remains the gold standard for evaluating the degree of hepatic necroinflammation and f ibrosis; however, several noninvasive investigations, such as serum biomarkers, have been developed to establish the diagnosis and also to evaluate treatment response. These markers are currently neither available in all centers nor validated in extensive studies. Examples include high-sensitivity C reactive protein and plasma pentraxin 3, which are associated with extensive liver f ibrosis in NASH. Interleukin-6 correlates with inflammation, and cytokeratin-18 represents a marker of hepatocyte apoptosis (prominent in NASH and absent in simple steatosis). Tissue polypep-tide specif ic antigen seems to have a clinical utility in the follow-up of obese patients with NASH.

关键词： Non-alcoholic fatty liver disease Biomarkers Necroinflammation Liver fibrosis

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Cancer stem cells: Involvement in pancreatic cancer pathogenesis and perspectives on cancer therapeutics

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World Journal of Gastroenterology 2014年第31期20卷 10790-10801页

作者： Cristiana Pistol Tanase ana Iulia Neagu laura Georgiana Necula Cristina Mambet ana-Maria Enciu Bogdan Calenic Maria Linda Cruceru Radu Albulescu Department of Biochemistry-Proteomics Victor Babes National Institute of Pathology Stefan S Nicolau Institute of Virology Carol Davila University of Medicine and Pharmacy National Institute for Chemical Pharmaceutical Research and Development

Pancreatic cancer is one of the most aggressive and lethal malignancies. Despite remarkable progress in understanding pancreatic carcinogenesis at the molecular level, as well as progress in new therapeutic approaches, pancreatic cancer remains a disease with a dismal prognosis. Among the mechanisms responsible for drug resistance, the most relevant are changes in individual genes or signaling pathways and the presence of highly resistant cancer stem cells(CSCs). In pancreatic cancer, CSCs represent 0.2%-0.8% of pancreatic cancer cells and are considered to be responsible for tumor growth, invasion, metastasis and recurrence. CSCs have been extensively studied as of late to identify specific surface markers to ensure reliable sorting and for signaling pathways identified to play a pivotal role in CSC self-renewal. Involvement of CSCs in pancreatic cancer pathogenesis has also highlighted these cells as the preferential targets for therapy. The present review is an update of the results in two main fields of research in pancreatic cancer, pathogenesis and therapy, focused on the narrow perspective of CSCs.

关键词： Cancer stem cells Pancreatic cancer Cancer stem cells signaling pathways Targeted therapy miRNA

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Gastrointestinal cancer stem cells as targets for innovative immunotherapy

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World Journal of Gastroenterology 2020年第14期26卷 1580-1593页

作者： Mihaela Chivu-Economescu laura G Necula Lilia Matei Denisa laura Dragu ana I Neagu Irina Alexiu Coralia Bleotu Carmen Cristina Diaconu Department of Cellular and Molecular Pathology Stefan S.Nicolau Institute of VirologyBucharest 030304Romania Nicolae Cajal Institute Titu Maiorescu UniversityBucharest 040441Romania

The role of cancer stem cells in gastrointestinal cancer-associated death has been widely *** cancer stem cells(GCSCs)are considered to be responsible for tumor initiation,growth,resistance to cytotoxic therapies,recurrence and metastasis due to their unique *** properties make the current therapeutic trials against GCSCs ***,recent studies have shown that targeting stem cell surface markers or stemness associated pathways might have an additional off-target effect on the immune *** advances in oncology and precision medicine have opened alternative therapeutic strategies in the form of cancer *** approach differs from classical anti-cancer therapy through its mechanism of action involving the activation and use of a functional immune system against tumor cells,instead of aiming physically destruction of cancer cells through radio-or *** immunological approaches for GCSCs targeting involve the use of different immune cells and various immune mechanisms like targeting specific surface antigens,using innate immune cells like the natural killer and T cells,T-cell chimeric antigen receptor technology,dendritic cell vaccine,or immune checkpoint *** this respect,better understandings of immune regulatory mechanisms that govern anti-tumor response bring new hope in obtaining long-term remission for cancer therapy.

关键词： Immunotherapy Gastrointestinal cancer Cancer stem cells CAR-T Dendritic cells vaccines Immune checkpoints inhibitors

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Application of formal languages in polynomial transformations of instances between NP-complete problems

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第8期14卷 623-633页

作者： Jorge A. RUIZ-VANOYE Joaquín PREZ-ORTEGA Rodolfo A. PAZOS RANGEL Ocotlán DíAZ-PARRA Héctor J. FRAIRE-HUACUJA Juan FRAUSTO-SOLíS Gerardo reyes-SALGADO laura CRUZ-reyes DACI Universidad Autónoma del Carmen Computer Science Centro Nacional de Investigación y Desarrollo Tecnológico Systems and Computer Science Instituto Tecnológico de Ciudad Madero Informatics Universidad Politécnica del Estado de Morelos Computer Science Instituto Tecnológico de Cuautla

We propose the usage of formal languages for expressing instances of NP-complete problems for their application in polynomial transformations. The proposed approach, which consists of using formal language theory for polynomial transformations, is more robust, more practical, and faster to apply to real problems than the theory of polynomial transformations. In this paper we propose a methodology for transforming instances between NP-complete problems, which differs from Garey and Johnson's. Unlike most transformations which are used for proving that a problem is NP-complete based on the NP-completeness of another problem, the proposed approach is intended for extrapolating some known characteristics, phenomena, or behaviors from a problem A to another problem B. This extrapolation could be useful for predicting the performance of an algorithm for solving B based on its known performance for problem A, or for taking an algorithm that solves A and adapting it to solve B.

关键词： Formal languages Polynomial transformations NP-completeness

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阿塞洛米塔尔图巴朗钢铁集团火花发射光谱法优化氮的分析

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冶金分析 2012年第7期32卷 25-31页

作者： FAGUNDES laura DIEGUEZ ana Carolina 阿塞洛米塔尔图巴朗钢铁集团

近年来,钢铁行业需求量快速上升,竞争也变得更加激烈。为了得到性能可靠的产品,钢铁车间在生产工艺方面需要进一步严格控制好产品的生产规格。在这种背景下,为了严格控制好生产工艺的密封性并保证产品所期望的特性(尤其是其韧性和机械... 详细信息

近年来,钢铁行业需求量快速上升,竞争也变得更加激烈。为了得到性能可靠的产品,钢铁车间在生产工艺方面需要进一步严格控制好产品的生产规格。在这种背景下,为了严格控制好生产工艺的密封性并保证产品所期望的特性(尤其是其韧性和机械加工性方面),精确地进行氮的分析必不可少。在过去几年中,经典燃烧技术(CC)已广泛应用于鉴定钢中的碳、硫和氮元素。而火花发射法因其性能改进、耗材成本(燃烧技术方面)降低以及在分析时间缩短等方面的优势,也在氮分析方面得到了应用,并且应用越来越广泛。本项研究的目的在于通过火花发射光谱仪(OES)对氮分析进行优化。研究是在巴西塞拉的阿塞洛米塔尔图巴朗钢铁集团的化学分析实验室进行,利用DMAIC方法学(一种用于改进生产工艺质量的研究方法)[1],对潜在的影响因素(包括漂移、样品和周期)进行了分析,并确定和优化了影响氮分析精度的根本原因。在实施该计划后,对结果的精度(基于内标物的标准偏差)和准确性(通过与OE认证的参考物质和力可交叉核验结果进行比较)进行了评估。在本研究项目之前,通过光发射法测得的氮元素的标准偏差高达0.004 0%。阿塞洛米塔尔图巴朗钢铁集团利用光谱仪完成所有的研究计划后,试验表明,其测定标准偏差低达0.000 5%,充分展现了该技术的潜力。本研究对这一标准偏差进行了监测,频率为每周一次,监测期为3个月。结果发现,该标准偏差在0.000 3%到0.000 5%之间波动,表明稳定性较好。由此可见,火花发射光谱技术是氮分析的一种较有前途的方法。

关键词：光发射氮分析钢铁经典燃烧

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Influence of surface roughness on the color of dental-resin composites

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第7期12卷 552-562页

作者： Razvan GHINEA laura UGARTE-ALVAN ana YEBRA Oscar E.PECHO Rade D.PARAVINA Maria del Mar PEREZ Department of Optics Faculty of ScienceUniversity of Granada Department of Stomatology Faculty of OdontologyUniversity of Granada Houston Center for Biomaterials and Biomimetics & Department of Restorative Dentistry and Biomaterials University of Texas Dental Branch at Houston

This study deals with the influence of surface roughness on the color of resin *** resin composites (microfilled,hybrid,and microhybrid) were each polished with 500-grit,1 200-grit,2 000-grit,and 4 000-grit SiC *** roughness parameter (Ra) was measured using a Plμ confocal microscope,and field-emission scanning electron microscope (Fe-SEM) images were used to investigate filler *** was measured using a spectroradiometer and a D65 standard illuminant (geometry diffuse/0° specular component excluded (SCE) mode).Surface roughness decreased with grit number and was not influenced by filler size or size distribution.A significant influence of Ra on lightness (L*) was *** increased with decreases in roughness,except for specimens that underwent polishing procedure 4 (PP4;500-grit,1 200-grit,2 000-grit,and 4 000-grit SiC papers consecutively).Generally,it was found that surface roughness influenced the color of resin *** composites that underwent PP1 (500-grit SiC paper) exhibited significant differences in chroma (C*),hue (h°),and lightness (L*) compared to composites that underwent PP3 (500-grit,1 200-grit,and 2 000-grit SiC papers consecutively) and *** difference (E*) between the polishing procedures was within acceptability thresholds in dentistry.

关键词： Color measurement Dental resin composites Roughness

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