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Case Report: Clinical and Diagnostic Aspects of Mayer-Rokitansky-Kuster-Hauser Syndrome

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Health 2019年第10期11卷 1367-1372页

作者： lucas Ferreira de Paula Filho Guilherme Carolino Neves Laís Figueira Bandoli lucas Caraline de Almeida Coelho Nathália de Souza Ferreira Feital Renan Vieira Murad ana Luísa Conceiç o de Jesus Diana Aristótelis de Sá Larissa Bianca Cunha de Sá Jonathan Mwambire Alberto Krayyem Arbex IPEMED Medical School/AFYA Educational Postgraduation in Endocrinology Rio de Janeiro Brazil UNIFESO Medical School Teresópolis Brazil Department of Pediatrics Medical School University of So Paulo (USP) So Paulo Brazil Medical Clinic in Endocrinology and Diabetology Schleswig-Flensburg Germany.

Mayer-Rokitansky-Kuster-Hauser Syndrome (MRKH) is a rare disease characterized by total or partial vagina agenesis, karyotype 46, XX with normal secondary sexual characters. Still, it is the second leading cause of primary amenorrhea. The absence of obvious signs and symptoms often causes the syndrome to be diagnosed only after puberty. The case presented here highlights exactly this difficulty of early diagnosis, which meets the objective of the study, and is precisely to provide reliable material that facilitates the diagnosis and management of patients with MRKH syndrome.

关键词： Mayer-Rokitansky-Kuster-Hauser Syndrome Primary Amenorrhea Gonadal Dysgenesis

Phenolic Compounds Influence Seed Dormancy of Palicourea rigida H.B.K.(Rubiaceae),a Medicinal Plant of the Brazilian Savannah

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American Journal of Plant Sciences 2013年第1期4卷 129-133页

作者： Marielle C.Inácio Rita Maria Moraes Patrícia C.Mendonca lucas J.F.Morel Suzelei C.Franca Bianca W.Bertoni ana M.S.Pereira Departamento de Horticultura Universidade Estadual Paulista Julio de Mesquita FilhoBotucatuBrazil National Center for Natu-ral Products Research School of PharmacyThe University of MississippiOxfordUSA Center for Water and Wetland Resources The University of MississippiAbbevilleUSA Departamento de Biotecnologia de Plantas Medicinais Universidade de Ribeirao PretoRibeirao PretoBrazil

Palicourea rigida H.B.K. (Rubiaceae), a medicinal species commonly known as douradinha, has wide distribution across ecosystems in Central and South America. This species exhibits seed dormancy delaying germination until optimal conditions for seedling growth and development are in place. While dormancy ensures species survival, it also presents a technical problem for developing P. rigida’s plant production program. Thus, the objective of this study was to investigate if secondary metabolites present in seeds influence the seed dormancy of P. rigida. Mature fruits were harvested from the native habitat, in the savanna region of the State of Minas Gerais during February 2009, 2010 and 2011. The content of phenolic compounds in the seed of P. rigida was measured, and the allelopathic effects were assessed using the germination of lettuces as model to detect phytotoxicity. The P. rigida seeds geminated at rates varying between 7% and 31% with a Seed Germination Index (SGI) of 0.09. Data suggest that the phenolic compounds present in the seeds may be responsible for seed dormancy.

关键词： Cerrado Douradinha Medicinal Plant Conservation

Effect of Domestic Wastewater as Co-Substrate on Biological Stain Wastewater Treatment Using Fungal/Bacterial Consortia in Pilot Plant and Greenhouse Reuse

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Journal of Water Resource and Protection 2018年第3期10卷 369-393页

作者： Pedroza-Camacho lucas David Lores-Acosta Juan Camilo Rojas-Enríquez Johans Farid Mateus-Maldonado Juan Felipe Puentes Cindy Stephanie Ramírez-Rodríguez Julio Mendez-Casallas Francy Janeth Salcedo-Reyes Juan Carlos Díaz-Ariza Lucía ana Lozano-Puentes Hair Santiago Pedroza-Rodríguez Aura Marina Programa de Biología. Departamento de Ciencias Básicas Universidad de La Salle Bogotá D.C. Colombia Facultad de Ingeniería Departamento de Ingeniería Ambiental Universidad de La Salle Bogotá D.C. Colombia Laboratorio de Microbiología Ambiental y de Suelos Grupo de Biotecnología Ambiental e Industrial Departamento de Microbiología Facultad de Ciencias Pontificia Universidad Javeriana Bogotá D.C. Colombia Laboratorio de Películas Delgadas y Nanofotonica Departamento de Física Facultad de Ciencias Pontificia Universidad Javeriana Bogotá D.C. Colombia Laboratorio Asociaciones suelo planta microorganismos (LAMIC) Grupo de Investigación en Agricultura Biológica Departamento de Biología Facultad de Ciencias Pontificia Universidad Javeriana Bogotá D.C. Colombia

In this study, a pilot wastewater treatment plant was used to evaluate the co-treatment of biological-staining residues and domestic wastewater under non-sterile conditions. A novel microbial consortia formed by Trametes versicolor, Trametes sp, Pleurotus ostreatus, Pseudomonas fluorescens, Pseudomonas azotoformans, Pseudomonas sp, Enterobacter xianfangensis and Bacillus subtillis was inoculated in an extended aeration type bio-reactor. The treatment units were operated during three consecutive cycles during a period of 147 h. After the last operating cycle, the concentrations of Chemical Oxygen Demand, Biochemical Oxygen Demand, Color Units, Total suspended solids, and the pH value were 1695 mg/L, 105 mg/L, 106 CU, 5), 1367 (CU), 566 mg/L (TSS) and 7.0 (pH) respectively. The reduction of pollutants load was related with the ratio of the two types of wastewater (3.5:0.5) combined to increase biodegradability, the concentration of fungi and bacteria used in the consortia (30 × 103 - 55 × 106 CUF/mL Total Fungi and 70 × 107 - 83 × 108 CFU/mL of Total Bacteria) and ligninolytic enzymes production, Laccase (13 - 96 U/L), MnP (9.8 - 39 U/L) and LiP (0.3 - 5.3 U/L). The post-treated effluent was used as irrigation water. Lolium perenne plants were watered during 60 days with post-treated effluent. The results of root weight showed that there are significant differences between the initial water and the effluent obtained after the operational cycles (p = 0.00470). The highest root weights (1 - 1.12 g) were found in plants irrigated with water obtained from the last treatment cycle.

关键词： Fungal/Bacterial Consortia Biological Staining and Domestic Wastewater Co-Treatment and Irrigation Reuse

Concentrations of Silver Nitrate in the In Vitro Development and Conservation of Passiflora gibertii ***

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American Journal of Plant Sciences 2017年第12期8卷 2944-2955页

作者： Gláucia Amorim Faria lucas Menezes Felizardo Antonio Flávio Arruda Ferreira Paula Suares Rocha Aline Namie Suzuki Antonio da Silva Souza Tatiana Góes Junghans Maria Angélica Pereira de Carvalho Costa ana Patricia Bastos Peixoto Augusto Ramalho de Morais Beatriz Garcia Lopes Tiago Almeida de Oliveira Departmentof Mathematics School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Department of Civil Engineering School of EngineeringSao Paulo State University(Unesp)Ilha SolteiraBrazil Laboratory of Environmental Mutagenesis Institute of BiosciencesSao Paulo State University(Unesp)Rio ClaroBrazil Embrapa Cassava and Fruticulture Cruz das AlmasBrazil Center for Agrarian Biological and Environmental SciencesFederal University of the Recôncavo of Bahia(UFRB)Cruz das AlmasBrazil Department of Statistics State University of Paraíba(UEPB)Campina GrandeBrazil Department of Exact Sciences Federal University of Lavras(UFLA)LavrasBrazil

The aim of this study was to evaluate the effects of silver nitrate in the in vitro development and conservation of passion fruit plants. Experiments were carried out at the Laboratory of Culture and Tissues, in the National Cassava and Fruits Research Center (EMBRAPA), using microcuttings of Passiflora gibertii N. E. Br., previously cultivated in vitro. The microcuttings were placed in MS media supplemented with silver nitrate at concentrations of 0, 1, 2, 4 and 8 mg·L-1, and, during 30, 60 and 90 days, the following variables were identified: Shooting length (cm), number of roots, number and coloration of leaves. A completely randomized design with 30 replications in a split-plot scheme was employed. Polynomial regression equations were adjusted in analysis of variance to compare averages of treatments. The obtained results support the assumption of high sensitivity of passion fruit tissues to ethylene, which reflects in the lost of vigor and in the induction of culture senescence by its accumulation. Based on obtained results, and in order to mitigate this problem, it is possible to indicate an addition of 2 mg·L-1?of silver nitrate in culture media, for the micropropagation of passion fruit plant, allowing the controlling of the atmosphere inside the culture test tubes, during the establishment of explants and other steps of the process.

关键词： Passion Fruits Tropical Fruits Plant Propagation Reduced Growth Ethylene

Characteristics relating to the interiorization of acquired immunodeficiency syndrome in Brazil: a cross-sectional study

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Infectious Diseases of Poverty 2015年第1期4卷 234-238页

作者： Gabriel de Deus Vieira ana Raquel Paz dos Reis Francisco Ormidiel Teles de Alcântara Augusto Karina Reis Martins Paulo Roberto Fernes Kern Thairini Fuza de Souza Sérgio de Almeida Basano Luís Marcelo Aranha Camargo Camila Maciel de Sousa Departament of Medicine São Lucas CollegePorto VelhoRondôniaBrazil Tropical Medicine Center of Rondônia Porto VelhoRondôniaBrazil Institute of Biomedical Sciences 5 University of São PauloMonte NegroRondôniaBrazil

Background:In recent years there has been changes in the social and geographic profile of acquired immunodeficiency syndrome(AIDS).The aim of this study was to evaluate the internalization of AIDS in the state of Rondônia,***:In Rondônia,1473 AIDS cases were reported,with an average annual incidence of 15.8/100,000 persons(42.7%women).The most common mode of viral transmission was sexual(96.5%),and the majority of the individuals had not completed their primary education(64.8%).There was heterogeneity in relation to case distribution,involving almost all of the municipalities in the *** average annual mortality rate was 2.5/100,000 ***:Rondônia has a higher incidence of AIDS than the national average and the northern *** to provide access to treatment and follow-up of these individuals should be implemented,prioritizing areas where the incidence is higher and decentralizing the treatment of patients with AIDS in the state.

关键词： Epidemiology Aids Residence characteristics Amazo

Kaphta:Text mining web tool to extract information on the anticancer activity of polyphenols

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Journal of Polyphenols 2022年第2期4卷 87-100页

作者： Ramon Gustavo Teodoro Marques da Silva Samuel lucas Santos Gomes Paulo Muniz deÁvila Gustavo Joséda Silva ana Lucia Fachin Edilson Carlos Caritá Mozart Marins Biotechnology Unit University of Ribeirão PretoAv.Costábile Romano 2201Ribeirão PretoSP 14096-900Brazil Federal Institute of Education Science and Technology of South of Minas Gerais–IFSULDEMINASPouso AlegreMG 37550-000Brazil Medicine School University of Ribeirão PretoRibeirão PretoSP 14096-900Brazil Centro de Ciências Exatas Naturais e TecnológicasUniversidade de Ribeirão PretoRibeirão Preto SP 14096-900Brazil

In this paper,we describe the application of Kaphta architecture,a resource for text mining of the anticancer activity of *** anticancer activity of these compounds against different types of cancer has been widely reported in the literature and they are one of the most promising molecules for the development of anticancer *** architecture,which comprises four sequential and well-defined steps,uses a hybrid approach composed of a dictionary,rules and machine learning to identify abstracts containing sentences with associations between polyphenol,cancer and gene *** application of the architecture on 23826 PubMed abstracts generated a knowledge base of indexed abstracts with 172169 sentences containing,polyphenol-cancer and polyphenol-gene associations.A Web tool was implemented that allowed the user to search for information on 2006 polyphenols,240 cancers and 3121 genes entities,and 11750 polyphenol-cancer and 9160 polyphenol-gene associations indexed in the knowledge base.A ranking algorithm calculates scores for each indexed abstract considering the number and type of sentences with entities and rules recognized.A test with users demonstrated that the visualization resources on the web tool contributes to the understanding of the association between polyphenols,genes and cancers,in comparison with the PubMed *** Kaphta architecture and web tool permits to extract knowledge on the anticancer activity of polyphenols and can thus contribute to the exploration of these molecules in the development of anticancer therapies.

关键词： polyphenols cancer text-mining