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8-Hydroxyquinolylnitrones as multifunctional ligands for the therapy of neurodegenerative diseases

Acta Pharmaceutica Sinica B 2023年第5期13卷 2152-2175页

作者： Damijan Knez Daniel Diez-Iriepa Mourad Chioua Andrea Gottinger Milica Denic Fabien Chantegreil Florian Nachon Xavier Brazzolotto Anna Skrzypczak-Wiercioch Ane Meden Anja Pilar Janko Kos Simon akelj Jure Stojan Kinga Saat Julia Serrano ana patricia fernández Aitana Sánchez-García Ricardo Martínez-Murillo Claudia Binda Francisco López-Muoz Stanislav Gobec JoséMarco-Contelles University of Ljubljana Faculty of PharmacyLjubljana 1000Slovenia Laboratory of Medicinal Chemistry(Institute of Organic Chemistry CSIC)Madrid 28006Spain Departamento de Química Orgánicay Química Inorgánica Alcaláde HenaresMadrid 28871Spain Department of Biology and Biotechnology University of PaviaPavia 27100Italy Département de Toxicologie et Risques Chimiques Institut de Recherche Biomédicale des ArméesParis 91220France Department of Animal anatomy and Preclinical Sciences University Centre of Veterinary Medicine JU-UAUniversity of Agriculture in KrakowKrakow 30-059Poland Institute of Biochemistry Faculty of MedicineUniversity of LjubljanaLjubljana 1000Slovenia Department of Pharmacodynamics Chair of PharmacodynamicsFaculty of PharmacyJagiellonian University Medical CollegeKrakow 30-688Poland Department of Translational Neurobiology Neurovascular Research GroupCajal Institute(CSIC)Madrid 28002Spain Biotechnology and Vegetal Biology Department Escuela Técnica Superior de Ingeniería AgronómicaAlimentaria y de BiosistemasUniversidad Politécnica de MadridMadrid 28040Spain Faculty of Health Camilo JoséCela University of Madrid(UCJC)Neuropsychopharmacology Unit“Hospital 12 de Octubre”Research InstituteMadrid 28692Spain

We describe the development of quinolylnitrones(QNs)as multifunctional ligands inhibiting cholinesterases(ChEs:acetylcholinesterase and butyrylcholinesterase—h BChE)and monoamine oxidases(hMAO-A/B)for the therapy of neurodegenerative diseases.We identified QN 19,a simple,low molecular weight nitrone,that is readily synthesized from commercially available 8-hydroxyquinoline-2-carbaldehyde.Quinolylnitrone 19 has no typical pharmacophoric element to suggest ChE or MAO inhibition,yet unexpectedly showed potent inhibition of h BChE(IC50=1.06±0.31 nmol/L)and h MAO-B(IC_(50)=4.46±0.18μmol/L).The crystal structures of 19 with hBChE and hMAO-B provided the structural basis for potent binding,which was further studied by enzyme kinetics.Compound 19 acted as a free radical scavenger and biometal chelator,crossed the blood—brain barrier,was not cytotoxic,and showed neuroprotective properties in a 6-hydroxydopamine cell model of Parkinson's disease.In addition,in vivo studies showed the anti-amnesic effect of 19 in the scopolamine-induced mouse model of AD without adverse effects on motoric function and coordination.Importantly,chronic treatment of double transgenic APPswe-PS1δE9 mice with 19 reduced amyloid plaque load in the hippocampus and cortex of female mice,underscoring the disease-modifying effect of QN 19.

关键词： Quinolylnitrone Butyrylcholinesterase Monoamine oxidase B Alzheimer's disease Multifunctional ligands 6-Hydroxydopamine model Passive avoidance task Novel object recognition

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Dietary(poly)phenol intake is associated with cardiometabolic health parameters in adolescents

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Food Science and Human Wellness 2024年

作者： Emily P.Laveriano-Santos Sara Castro-Barquero Camila Arancibia-Riveros ana María Ruiz-León Rosa Casas Ramón Estruch patricia Bodega Mercedes de Miguel Amaya de Cos-Gandoy Jesús Martínez-Gómez Gloria Santos-Beneit Juan M.fernández-Alvira Rodrigo fernández-Jiménez Rosa M.Lamuela-Raventós Anna Tresserra-Rimbau The Zena and Michael A.Wiener Cardiovascular Institute Icahn School of Medicine at Mount Sinai Department de Nutrició Ciències de l'Alimentació I Gastronomia XIA Facultat de Farmàcia i Ciències de l'alimentació Institut de Recerca en Nutrició i Seguretat Alimentària (INSA-UB) Universitat de Barcelona Department of Internal Medicine Hospital Clínic Institutd'Investigacions Biomèdiques August Pi I Sunyer (IDIBAPS) University of Barcelona Consorcio CIBER M.P.Fisiopatología de la Obesidad y Nutrición (CIBERObn) Instituto de Salud Carlos III (ISCIII) Mediterranean Diet Foundation 5. Foundation for Science Health and Education (SHE) 6. Centro Nacional de Investigaciones Cardiovasculares (CNIC) CIBER de Enfermedades Cardiovasculares (CIBERCV) 9. Hospital Universitario Clínico San Carlos

Background: The protective role of (poly)phenols against metabolic disorders has been extensively studied in adults but not in adolescents.Objective: To assess associations of dietary (poly)phenols and their subclasses with cardiometabolic health parameters in adolescents. Methods: A cross-sectional study was conducted in 944 individuals aged 11 to 14 years enrolled in the SI! Program for Secondary Schools trial (NCT03504059). (Poly)phenol intake was assessed using semi-quantitative food frequency questionnaires and the Phenol-Explorer database. The measured cardiometabolic parameters were waist circumference (WC) age-sex z-score， blood pressure (BP) age-sex z-score， blood glucose (BG)， triglycerides (TG)， and high-density lipoprotein cholesterol (HDL-c). Multilevel mixed-effect linear regression models were applied to examine the association between (poly)phenol quintiles and cardiometabolic health parameters.Results: Compared to the lowest quintile， adolescents in the highest quintile of total (poly)phenol intake had lower WC z-scores (β=-0.09， 95%CI=-0.09;-0.08， P-for trend=0.002)， mean arterial pressure z-scores (β =-0.11， 95%CI=-0.12;-0.09， P-for trend=0.206)， and HDL-c (β=-1.52， 95%CI=-2.90;-0.14， P-for trend=0.108)， after multivariable adjustment. The WC z-scores (β=-0.11， 95%CI=-0.19;-0.03， P-for trend=0.013) and HDL-c (β=-2.16， 95%CI=-3.78;-0.53， P-for trend=0.046) were lower in the highest quintile of flavonoid intake compared to the lowest quintile. The highest quintile of phenolic acid intake was associated with a lower WC z-score (β=-0.14， 95%CI=-0.26;-0.02， P-for trend=0.011) and TG levels (β=-8.83， 95%CI=-12.37;-5.30， P-for trend <0.001)， and the highest quintile of stilbene intake with lower BG (β=-1.83， 95%CI= -3.103;-0.64， P-for trend <0.001) and TG (β=-3.22， 95%CI= -4.95;-1.49， P-for trend=0.034)， and with higher HDL-c (β=2.328， 95%CI= 1.40;3.17， P-for trend=0.466) compared to the lowest quintile. Conclusion: A higher intake of (poly)phenols， especially flavonoids， phenolic acids， and stilbenes

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Vanadium mineralization at Los Chihuidos sediment-hosted Cu-V deposit,Neuquén Basin,Argentina:An approach to vanadium ore forming processes

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Geoscience Frontiers 2024年第1期15卷 225-246页

作者： ana L.Rainoldi Daniel Beaufort Marta B.Franchini Adolfo Giusiano Sabine Petit patricia Patrier M.Josefina Pons Dpto.de Geología Universidad Nacional del Sur(UNS)8000 Bahía BlancaArgentina Consejo Nacional de Investigaciones Científicas y Técnicas Centro Patagónico de Estudios MetalogenéticosArgentina Universitéde Poitiers IC2MPCNRS-UMR 7285HydrasaF-86022 Poitiers CedexFrance Universidad Nacional del Comahue Facultad de IngenieríaDepartamento de Geología y Petróleo8300 NeuquénArgentina Consultant Geologist 8300 NeuquénArgentina Universidad Nacional de Río Negro Instituto de Investigación en Paleobiología y GeologíaRío NegroArgentina

Vanadium mineralization at Los Chihuidos deposit of the Neuquén Basin is linked to the development of a redox front system related to the inflow of hydrocarbons into the red sandstone of the Huincul Formation.Interaction of hydrocarbons with oxidized red beds and connate water generated redox reactions where hematite was dissolved due to iron reduction resulting in the discoloration of the red strata.At the contact between oxidized red sandstone and reduced white sandstone,precipitation of specific mineral phases resulted in the V ore with minor amounts of Cu.With the implementation of the redox interface,abundant V-montmorillonite and V-hematite precipitated at the more oxidizing conditions and Cu-V-corrensite-type at the more reducing conditions of the redox front.As the redox front advanced with fluids constantly migrating into the reservoir,more reducing conditions were stablished,promoting chloritization and minor illitization with V-Cu incorporation and continuous upgrading of the ore.Main ore mineralogy consists of clay minerals including V-bearing montmorillonite,Cu-V-corrensite-type,V-di-trioctahedral chlorite and Cu-tri-trioctahedral chlorite with minor V-illite-smectite mixed-layer minerals and associated secondary V-hematite.Chloritization over illitization was favored due to high amounts of Fe and Mg in detrital clasts and in connate fluids and by low K availability related to low amounts of detrital K-feldspar.The spatial transition of V and/or Cu bearing clay minerals observed through the mineralized redox front at Los Chihuidos deposit(kaolinite→smectite→illite/smectite→corrensite-type→di-trioctahedral-chlorite→tri-trioctahedral-chlorite)and the related variation of V-Cu concentrations in bulk rock are indicative of increasing pH and decreasing Eh of resident solutions from red to white sandstones during the hypogene mineralization process.Late influx of Cu-rich oxidized basinal brines precipitated main copper ore with Cu-sulfides in the white sandstone up to the contact with the redox front in co

关键词： Clay minerals V-chlorite Redox front Bleaching Hydrocarbons

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Host-parasite interactions in perpetual darkness:Macroparasite diversity in the cavefish Astyanax mexicanus

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Zoological Research 2023年第4期44卷 782-792页

作者： ana Santacruz David Hernández-Mena Ramses Miranda-Gamboa Gerardo Pérez-Ponce De León Claudia patricia Ornelas-García Departamento de Zoología Instituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico City CP 04510México Departamento de Sistemas y Procesos Naturales Escuela Nacional de Estudios Superiores Unidad MéridaUcúYucatán CP 97357México Instituto de Energías Renovables Universidad Nacional Autónoma de MéxicoTemixcoMorelos CP 62580México Colección Nacional de Peces Departamento de ZoologíaInstituto de BiologíaUniversidad Nacional Autónoma de MéxicoMéxico City CP 04510México Stowers Institute for Medical Research Kansas CityMO 64110USA

Astyanax mexicanus has repeatedly colonized cave environments,displaying evolutionary parallelisms in many troglobitic traits.Despite being a model system for the study of adaptation to life in perpetual darkness,the parasites that infect cavefish are practically unknown.In this study,we investigated the macroparasite communities in 18 cavefish populations from independent lineages and compared them with the parasite diversity found in their sister surface fish populations,with the aim of better understanding the role that parasites play in the colonization of new environments.Within the cavefish populations,we identified 13 parasite taxa,including a subset of 10 of the 27 parasite taxa known for the surface populations.Parasites infecting the cavefish belong to five taxonomic groups,including trematodes,monogeneans,nematodes,copepods,and acari.Monogeneans are the most dominant group,found in 14 caves.The macroparasites include species with direct life cycles and trophic transmission,including invasive species.Surprisingly,paired comparisons indicate higher parasite richness in the cavefish than in the surface fish.Spatial variation in parasite composition across the caves suggests historical and geographical contingencies in the host-parasite colonization process and potential evolution of local adaptations.This base-line data on parasite diversity in cavefish populations of A.mexicanus provides a foundation to explore the role of divergent parasite infections under contrasting ecological pressures(cave vs.surface environments)in the evolution of cave adaptive traits.

关键词： Adaptation Metazoan parasites Niche change Parasite assemblage Prevalence

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Differential diagnosis between autism spectrum disorder and other developmental disorders with emphasis on the preschool period

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世界儿科杂志:英文版 2023年第8期19卷 715-726页

作者： Taynara Amancio de Lima patricia Aparecida Zuanetti Marina Estima Neiva Nunes ana Paula Andrade Hamad Department of Neurosciences and Behavioral Sciences Ribeirão Preto Medical SchoolUniversity of São PauloAv.Bandeirantes3900Monte AlegreRibeirão PretoSP14049-900Brazil University Hospital Ribeirão Preto Medical SchoolUniversity of São Paulo(HCFMRP-USP)Ribeirão PretoSPBrazil

Background Neurodevelopmental disorders are a heterogeneous group of conditions that manifest as delays or deviations in the acquisition of expected developmental milestones and behavioral changes.Autism spectrum disorder(ASD)is a neurodevelopmental disorder characterized by deficits in communication and social interaction and by repetitive and restricted patterns of behavior,interests and activities.The aim of this review is to discuss the clinical features of the differential diagnoses of ASD that are prevalent among preschoolers,focusing on their similarities and disparities.Data sources The international medical literature search was conducted using PubMed and was revised regarding the subject using single and/or combined keywords as follows:differential diagnosis,preschoolers,diagnostic challenge,attention deficit hyperactivity disorder,intellectual disability,high abilities/giftedness,childhood apraxia of speech,social communication disorder,Landau–Kleffner syndrome,stereotyped movement disorder and excessive screen time.Results We describe conditions commonly found in clinical practice,taking ASD as a reference.We addressed converging and divergent aspects of behavior,cognition,communication,language,speech,socialization,and stereotypes for the diagnosis of ASD and other disorders identified as potential differential or comorbid diagnoses.Conclusions The ranking and characterization of symptoms appear to be essential for better understanding the underlying common ground between children with developmental disorders and children with ASD,thus properly diagnosing and directing social,professional,or medication interventions.This detailed discussion adds to the literature since,although ASD differential diagnoses are frequently mentioned and discussed in textbooks and journal articles,they rarely occupy a prominent place as we aimed herein.

关键词： Autism spectrum disorder Clinical features Differential diagnosis Neurodevelopmental disorders Preschool

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Pancreatectomy with concomitant portal vein resection in the current neoadjuvant era

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Hepatobiliary Surgery and Nutrition 2022年第2期11卷 295-298页

作者： Benedetto Ielpo fernando Burdio ana Martinez patricia Sanchez-Velazquez Hepato-Biliary and Pancreatic Surgery Unit Hospital del MarUniversitat Pompeu FabraBarcelonaSpain

I’ve read with great interest the paper of Machairas et al.(1)which is of great interest for the oncological and surgical community.Pancreatic adenocarcinoma(PDAC)is still considered a malignancy with an extremely poor prognosis,being currently the 4th cause of death for cancer and,according to the Global Cancer Statistics in the next decade,it may ascend up to the third cause of death(2).During the last decades,there have been great efforts to improve survival of potentially resectable pancreatic cancer through several improvements.Firstly,the selection of patients:newer preoperative studies have been able to select those patients that might really benefit from resection.Secondly,the surgical procedure itself improved its outcome:proper mesopancreatic dissection and lymphadenectomy,superior mesenteric artery approach and vascular resection have been able to enhance the R0 resection rate with acceptable morbidity(3).Finally,newer chemotherapies regimens have been introduced,as well,with relevant survival improvement(4).

关键词： resection death

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加夫列尔·加西亚·马尔克斯图书馆,巴塞罗那,西班牙

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世界建筑 2024年第5期 76-81页

作者：无 Elena Orte Guillermo Sevillano Marta Romero Jesús Lopez Luis Sierra ana patricia Minguito Pablo Corroto María Abellán Sara Contreras RitaÁlvarez Tabio MiguelÁngel Maure Jesús Granada(摄影) SUMA建筑设计事务所不详

加布里埃尔·加西亚·马尔克斯图书馆是巴塞罗那的第40座图书馆,也是巴塞罗那30年计划的结晶。该计划旨在建立一个由优秀社区与区域设施组成的密集网络。这座图书馆位于圣马蒂德普罗旺斯区,为53,000人提供服务。关联式建筑图书馆被设计... 详细信息

加布里埃尔·加西亚·马尔克斯图书馆是巴塞罗那的第40座图书馆,也是巴塞罗那30年计划的结晶。该计划旨在建立一个由优秀社区与区域设施组成的密集网络。这座图书馆位于圣马蒂德普罗旺斯区,为53,000人提供服务。关联式建筑图书馆被设计成一座雕塑式实体建筑,让人联想到堆叠的书籍及其折叠的书页。该馆坐落在一处高架广场上,方便周围行人来往。同时,它又在空地上悬挑而出,门廊由现有的树木环绕,形成了一座城市大厅。不同大小及结构的开口定义了其不同立面与周围环境的关系。

关键词：巴塞罗那普罗旺斯周围环境加布里埃尔加西亚·马尔克斯图书馆关联式西班牙

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Relevance of Family in Academic Success on Children

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Health 2017年第2期9卷 279-288页

作者： patricia Martínez Lanz ana Cristina Losada fernández Fabiola Pecchioni fernández Martha patricia Hernández Valdez Universidad Aná huac Mé xico M&eacute xico City M&eacute xico

The purpose of this study was to analyze the relationship between academic success and functional and dysfunctional families. A non-experimental, cross-sectional, descriptive correlational design was used. A convenience sample of 200 children between 6 and 13 years old, primary and junior high school students was conducted (32.3% were female and 67.7% were male). To assess the relationship, a study case of 21 reagents was designed to empty the obtained information from medical review records from a psychopedagogic clinic. Academic success was measured as from the revision of informal tests and standardized tests. Results showed that the population levels of academic success were average. However, a significant relationship between the age of the parents and the level of academic success was found, indicating that these variables are associated with this problem. Besides that, it was found that there is a tendency in children with cognitive, emotional and sensory problems reflected in low academic success. The findings provide information that could be considered for the development of programs of psychopedagogic intervention to treat children with learning disabilities and low academic success.

关键词： Academic Success Low Academic Success Functional Family Dysfunctional Family Children

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P2X7 receptor blockade decreases inflammation,apoptosis,and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

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World Journal of Gastroenterology 2022年第30期28卷 4075-4088页

作者： ana A Q A Santos Deiziane V S Costa Danielle A Foschetti Antoniella S G Duarte Conceicao S Martins Pedro M G Soares patricia Castelucci Gerly A C Brito Department of Morphology School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Physiology and Pharmacology School of MedicineFederal University of CearáFortaleza 60430-170CearaBrazil Department of Pathology and Legal Medicine School of MedicineFederal University of CearaFortaleza 60430-170CearaBrazil Department of Morphology(UFC) Federal University of CearaFortaleza 60430-170CearaBrazil Department of anatomy Institute of Biomedical SciencesUniversity of São PauloSao Paulo 05508-270Brazil Department of Morphology Federal University of CearaFortaleza 60140-170CearaBrazil

Clostridioides difficile(C.difficile)is the most common pathogen causing health care-associated infections.C.difficile TcdA and TcdB have been shown to activate enteric neurons;however,what population of these cells is more profoundly influenced and the mechanism underlying these effects remain unknown.AIM To characterize a specific population of TcdA-affected myenteric neurons and investigate the role of the P2X7 receptor in TcdA-induced ileal inflammation,cell death,and the changes in the enteric nervous system in mice.METHODS Swiss mice were used to model TcdA-induced ileitis in ileal loops exposed to TcdA(50μg/Loop)for 4 h.To investigate the role of the P2X7 receptor,Brilliant Blue G(50 mg/kg,i.p.),which is a nonspecific P2X7 receptor antagonist,or A438079(0.7μg/mouse,i.p.),which is a competitive P2X7 receptor antagonist,were injected one hour prior to TcdA challenge.Ileal samples were collected to analyze the expression of the P2X7 receptor(by quantitative real-time polymerase chain reaction and immunohistochemistry),the population of myenteric enteric neurons(immunofluorescence),histological damage,intestinal inflammation,cell death(terminal deoxynucleotidyltransferasemediated dUTP-biotin nick end labeling),neuronal loss,and S100B synthesis(immunohistochemistry).RESULTS TcdA upregulated(P<0.05)the expression of the P2X7 receptor gene in the ileal tissues,increasing the level of this receptor in myenteric neurons compared to that in control mice.Comparison with the control mice indicated that TcdA promoted(P<0.05)the loss of myenteric calretinin+(Calr)and choline acetyltransferase+neurons and increased the number of nitrergic+and Calr+neurons expressing the P2X7 receptor.Blockade of the P2X7 receptor decreased TcdAinduced intestinal damage,cytokine release[interleukin(IL)-1β,IL-6,IL-8,and tumor necrosis factor-α],cell death,enteric neuron loss,and S100B synthesis in the mouse ileum.CONCLUSION Our findings demonstrated that TcdA induced the upregulation of the P2X7 receptor,which promoted enteric neuron loss,

关键词： Clostridioides difficile Clostridioides difficile toxin A P2X7 receptor Enteric nervous system Enteric neuron Enteric glia

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Roots and rhizomes of wild Asparagus:Nutritional composition,bioactivity and nanoencapsulation of the most potent extract

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Food Bioscience 2022年第1期45卷 349-360页

作者： Khaoula Adouni ana Júlio Celestino Santos-Buelga ana M.Gonzalez-Paramas Patrícia Filipe patricia Rijo Sofia A.Costa Lima Salette Reis Angela fernandes Isabel C.F.R.Ferreira Virginia fernandez-Ruiz patricia Morales Guido Flamini Lotfi Achour Pedro Fonte Laboratoire de Recherche“Bioressources:Biologie Integrative&Valorisation” Institut Superieur de Biotechnologie de MonastirAvenue Tahar HaddedBP 74Universite de Monastir5000MonastirTunisia CBIOS-Research Center for Biosciences&Health Technologies Universidade Lusofona de Humanidades e TecnologiasCampo Grande 3761749-024LisbonPortugal Department of Biomedical Sciences University of Alcal´aCtra.Madrid-Barcelona Km.33.60028871Alcala de HenaresMadridSpain Grupo de Investigacion en Polifenoles(GIP-USAL) Facultad de FarmaciaUniversidad de SalamancaCampus Miguel de UnamunoE-37007SalamancaSpain Research Institute for Medicines(iMed.ULisboa) Faculty of PharmacyUniversidade de LisboaAv.Professor Gama Pinto1649-003LisboaPortugal LAQV REQUIMTEDepartment of Chemical Sciences–Applied Chemistry LabFaculty of Pharmacy10 University of PortoRua Jorge de Viterbo Ferreira 2284050-313PortoPortugal Centro de Investigacao de Montanha(CIMO) Instituto Politecnico de BragancaCampus de Santa Apolonia5300-253BragancaPortugal Department of Nutrition and Food Science Faculty of PharmacyComplutense University of Madrid(UCM)Plaza Ramon y Cajal s/n28040MadridSpain Dipartimento di Farmacia University of Pisavia Bonanno 656126PisaItaly Centro Interdipartimentale di Ricerca“Nutraceutica e Alimentazione per la Salute”University of Pisa Via del Borghetto 8056124PisaItaly Center for Marine Sciences(CCMar) University of AlgarveGambelas Campus8005-139FaroPortugal Department of Chemistry and Pharmacy Faculty of Sciences and TechnologyUniversity of AlgarveGambelas Campus8005-139FaroPortugal iBB-Institute for Bioengineering and Biosciences Department of BioengineeringInstituto Superior TecnicoUniversidade de Lisboa1049-001LisboaPortugal Associate Laboratory i4HB—Institute for Health and Bioeconomy at Instituto Superior Tecnico Universidade de LisboaAv.Rovisco Pais1049-001LisboaPortugal

The nutritional composition and bioactive properties of roots and rhizomes of Asparagus stipularis were evaluated.Antioxidant activity of extracts obtained by infusion was evaluated using free radicals scavenging and reducing power methods.Porcine liver primary cell was used to check the hepatotoxicity of infusions.Results revealed that Asparagus samples are likely a source of nutrients,such as dietary fibre and essential fatty acids.HPLC-DAD-ESI/MS characterization of infusions allowed the identification and quantitation of 7 phenolic compounds,all hydroxycinnamoyl derivatives,with caffeic acid as the most abundant.Roots infusion contained the highest amounts of these compounds.It also exhibited the highest antioxidant activity in all assays,with EC_(50) values of 0.44±0.01,0.98±0.03 and 0.64±0.01 mg/mL for DPPH,ABTS and FRAP assays,respectively,with no toxicity towards PLP2 primary cell cultures(GI_(50)>400μg/mL).PLGA nanoparticles loaded with root extract were prepared using solvent-evaporation double emulsion method.Nanoparticles size was about 260 nm and a polydispersity index around 0.1,with a zeta potential of about-36 mV,as well as a good encapsulation efficiency of approximately 83%.Their morphology was analysed by SEM and spherical polymeric nanoparticles with a smooth surface were observed.FTIR and DSC were also performed,which allowed corroborating the efficacy of the encapsulation and to confirm the production of a stable and robust system to load Asparagus extracts.The developed nanoparticles are expected to be used as delivery systems for bioactive compounds of A.stipularis and they could be used as an innovative dietary supplement.

关键词： Asparagus stipularis Nutritional composition Antioxidant activity Nanoparticles PLGA

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