检索结果-南通市图书馆

Early detection of tomato bacterial canker by reflectance indices

在线全文

学校读者我要写书评

暂无评论

Information Processing in Agriculture 2022年第2期9卷 184-194页

作者： Gabriela Cordon carolina Andrade Lucia Barbara ana Maria Romero Universidad de Buenos Aires Consejo Nacional de Investigaciones Cientı´ficas y Te´cnicasInstituto de Investigaciones Fisiolo´gicas y Ecolo´gicas Vinculadas a la Agricultura(IFEVA)Facultad de Agronomı´aBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aA´rea de Educacio´n AgropecuariaBuenos AiresArgentina Universidad de Buenos Aires Facultad de Agronomı´aCa´tedra de Fitopatologı´aBuenos AiresArgentina

Early detection of infected asymptomatic plants could prevent the dissemination of tomatobacterial canker throughout the crop and, thus, reduce associated economic losses. Reflectance spectroscopy has been proposed for the detection of diseases in plants, mainlycaused by foliar fungal pathogens. In contrast, bacterial canker is a vascular disease thatdoes not produce visible foliar symptoms until it is too late to avoid contagion to otherplants. The effects of the disease on the conductivity of the xylem have an impact onthe water content of the leaves;initially, a subtle effect that cannot be assessed with thenaked eye but can be evaluated by reflectance spectroscopy. The objective of this workwas to identify indices, based on the reflectance spectral signature of the plants, for thedetection of tomato plants affected by bacterial canker prior to the appearance of symptoms. To verify that the proposed methodology is applicable to a broad range of tomatogenotypes, two experiments were carried out with different tomato cultivars grown in potsand another on the ground in a greenhouse. Plants were inoculated with the pathogenicbacteria and the reflectance spectrum of the apical leaflet of two leaves per plant wereobtained before symptoms expression;several spectral indices were calculated from thereflectance spectra. Three indices, of the shortwave-infrared zone, allowed the detectionof bacterial canker inoculated plants in a fast and non-destructive way, up to one weekbefore symptoms were visible: Normalized Difference Water Index (NDWI), Simple Ratioof Water Index (SRWI) and Water Index 1 180 (WI1180). Our research demonstrates, forthe first time, the usefulness of spectral indices sensitive to water content for the identifi-cation of diseased plants. These indices arise as a promising tool for the early detection ofvascular plant diseases, that could be integrated into sustainable management plans.

关键词： Clavibacter michiganensis subsp. michiganensis Bacterial wilt Reflectance indices Solanum lycopersicum L

Proactive reconciliation as a tool for integrating mining and milling operations

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Mining Science and Technology 2019年第2期29卷 239-244页

作者： ana carolina Chieregati Luiz Eduardo Campos Pignatari Francis Fernand Pitard Homero Delboni Jr Department of Mining and Petroleum Engineering University of Sao Paulo EDEM Engenharia Mineral Francis Pitard Sampling Consultants

Historically, reconciliation has been viewed as a quality test of model estimates as well as a powerful tool for detecting and correcting problems in all stages of mine operations from resource estimation to metal production. If used correctly, reconciliation helps to better predict the life of mine(LOM), improves the adherence of production plans to the respective budget, and allows for effective control of the mining and milling processes. However, the accuracy of reconciliation results requires that all input data must be generated in accordance with the principles of sampling correctness. Furthermore, complete reconciliation systems can become extremely complex and must be carefully analyzed in order to provide realistic and helpful conclusions. Based on these concepts, this paper presents a successful proactive reconciliation system applied to a copper and gold mine in Brazil for monitoring and integrating mining and milling operations.

关键词： Reconciliation Prognostication Sampling Gold Copper

Y-chromosome microdeletions in nonobstructive azoospermia and severe oligozoospermia

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2017年第3期19卷 338-345页

作者： carolina goncalves Mariana Cunha Eduardo Rocha Susana Fernandess, Joaquina Silva Luis Ferraz Cristiano Oliveira Alberto Barros Mairio Sousa Department of Biology ClCECO University of Aveiro Campus Universitario de Santiago 3810-193 Aveiro Portugal Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar University of Porto (ICBAS-UP) Rua Jorge Viterbo Ferreira 228 4050-313 Porto Portugal and Multidisciplinary Unit for Biomedical Research ICBAS-UP Portugal Centre for Reproductive Genetics Prof. Alberto Barros Av. do Bessa 240 1° Dto. Frente 4100-009 Porto Portugal Department of Microscopy Laboratory of Histology and Embryology ICBAS-UP Rua Jorge Viterbo Ferreira 228 4050-313 Porto Portugal Department of Genetics Faculty of Medicine University of Porto Alameda Prof. Hernani Monteiro 4200-319 Porto Portugal and Institute for Innovation and Health Research (13S) University of Porto Portugal Department of Urology Hospital Center of Vila Nova de Gala Rua Conceicao Fernandes 4430-502 Vila Nova de Gala Portugal.

The aim of the present work was to present the outcomes of the patients with Y-chromosome microdeletions treated by intracytoplasmic sperm injection （ICSI）, either using fresh （TESE） or frozen-thawed （TESE-C） testicular sperm and ejaculated sperm （EJAC）. The originality of this work resides in the comparisons between the different types of Y-microdeletions （AZFa, AZFb, and AZFc） and treatments, with detailed demographic, stimulation, embryological, clinical, and newborn （NB） outcomes. Of 125 patients with Y-microdeletions, 33 patients presented severe oligozoospermia （18 performed ICSI with ejaculated sperm） and 92 secretory azoospermia （65 went for TESE with 40 having successful sperm retrieval and performed ICSI）. There were 51 TESE treatment cycles and 43 TESE-C treatment cycles, with a birth of 19 NB （2 in AZFa/TESE-C, 12 in AZFc/TESE, and 5 in AZFc/TESE-C）. Of the 29 EJAC cycles, there was a birth of 8 NB （in AZFc）. In TESE and EJAC cycles, there were no significant differences in embryological and clinical parameters. In TESE-C cycles, there was a significant lower oocyte maturity rate, embryo cleavage rate and mean number of embryos transferred in AZFb, and a higher mean number of oocytes and lower fertilization rate in AZFc. In conclusion, although patients with AZFc microdeletions presented a high testicular sperm recovery rate and acceptable clinical outcomes, cases with AZFa and AZFb microdeletions presented a poor prognosis. Due to the reported heredity of microdeletions, patients should be informed about the infertile consequences on NB and the possibility of using preimplantation genetic diagnosis for female sex selection.

关键词： intracytoplasmic sperm injection newborn outcomes nonobstructive azoospermia severe oligozoospermia testicularsperm extraction Y-chromosome microdeletions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

棒与星系整体恒星形成性质关系的统计研究

天文学进展 2024年第1期42卷 115-127页

作者：穆子豪沈世银 Rafael S.de Souza ana L.Chies-Santos 中国科学院上海天文台星系宇宙学重点实验室上海200030 中国科学院大学北京100049 星系和宇宙学半解析研究重点实验室上海200034 赫特福德大学天体物理研究中心英国哈特菲尔德学院巷AL109AB 南里奥格兰德州联邦大学科学研究所巴西阿雷格里港9500

盘状星系中的棒结构特征与星系自身所处环境及整体恒星形成特征之间存在复杂的相互关系。利用细致的星系形态特征测量计划Galaxy Zoo DECaLS中的数据构建了一组目前最大的具有同样恒星质量分布的强棒、弱棒和无棒星系样本。通过引入星... 详细信息

盘状星系中的棒结构特征与星系自身所处环境及整体恒星形成特征之间存在复杂的相互关系。利用细致的星系形态特征测量计划Galaxy Zoo DECaLS中的数据构建了一组目前最大的具有同样恒星质量分布的强棒、弱棒和无棒星系样本。通过引入星系所处暗晕质量及相空间特征来控制星系所处的环境效应之后,进一步对比研究强棒、弱棒和无棒星系的恒星形成特征。研究表明,有棒星系中的恒星形成熄灭的比例系统地高于无棒星系,且强棒的效应更为明显。但是,对于仍处于恒星形成主序上的星系来说,强棒、弱棒和无棒星系的恒星形成特征并没有显著差别。以上结果表明,星系中的棒特征在其存在的时标中可能使部分星系迅速地从恒星形成转入熄灭状态。

关键词：棒旋星系恒星形成环境

First Serological Evidence on Endemicity of HEV Infection in Wild Boar(Sus scrofa) Populations from Portugal

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2018年第2期33卷 197-200页

作者： Daniel goncalves Joao Pereira-Vaz Vitor Duque Victor Bandeira Carlos Fonseca ana Donato Cristina Luxo ana Miguel Matos Laboratory of Virology Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Laboratory of Molecular Biology Clinical Pathology UnitHospital and University Centre of Coimbra3000-602 Coimbra Portugal Infectious Diseases Department Hospital and University Centre of Coimbra 3000-075 Coimbra Portugal Biology Department and CESAM University of Aveiro3810-193 Aveiro Portugal Clinical Laboratory Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal Research Centre on Chemical Processes Engineering and Forest Products (CIEPQF) Faculty of Pharmacy University of Coimbra 3030-790 Coimbra Portugal

Increasing number of hepatitis E virus（HEV）infection has been described in industrialized countries,with infection occurring as a sporadic disease,usually associated with travel to endemic regions or small outbreaks... 详细信息

关键词：感染 HEV 血清学葡萄牙野猪证据特性人口

A long-term record of land use change impacts on sediments in Oualidia lagoon,Morocco

在线全文

学校读者我要写书评

暂无评论

International Journal of Sediment Research 2014年第1期29卷 1-10页

作者： Mehdi MAanaN ana carolina RUIZ-FERNNDEZ Mohamed MAanaN Paul FATTAL Bendahhou ZOURARAH Mohamed SAHABI Géolittomer UMR 6554 LETG Université de Nantes Marine Geosciences Laboratory URAC 45Earth Sciences departmentFaculty of Sciences Universidad Nacional Autónoma de México.Instituto de Ciencias del Mary Limnología

An integrated approach,involving land use patterns and heavy metal contents of the surficial and cored sediment,was proposed to detect the characteristic spatial and temporal scale of point and non-point source human disturbances on the Oualidia lagoon in *** and description of the temporal and spatial distributions of the main contamination sources of heavy metals are analyzed using statistics and GIS *** results show that surficial sediments are highly enriched in heavy metals compared to their preindustrial background ***-use types affected lagoon sediment pollution in different ways:1) agricultural areas had the highest potential for sediment contamination by heavy metals,particularly Pb,Cu and Hg,2) Ni and Cr are the main pollutants originating from urban sewage and 3) oyster farming and traditional land uses such as salt flats,pastures showed low levels of others *** enrichment factors(normalized by Al) obtained from the sediment cores indicated that the lagoon is(strongly/moderately) polluted by all metals which were attributed to agricultural activities and wastewater discharges from urbanized areas.

关键词： Land use change(LUC) Geographical information systems(GIS) Sediment pollution Risk assessment

High passage numbers induce resistance to apoptosis in C2C12muscle cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2013年第1期37卷 1-9页

作者： LUCIA PRONSATO anaBELA LA COLLA ana carolina RONDA LORENA MILANESI RICARDOBOLAND ANDREA VASCONSUELO Departamento de Biología Bioquímica y FarmaciaUniversidad Nacional del Sur.San Juan 6708000 Bahía BlancaArgentina

Cell lines with high passage numbers exhibit alterations in cell morphology and *** thepresent work,C2C12 skeletal muscle cells with either low(60)passage numbers(identified as l-C2C12 or h-C2C12,respectively)were used to investigate the apoptotic response to H2O2 as a function ofculture age *** found that older cultures(h-C2C12 group)were depleted of mitochondrial DNA(mtDNA).When we analyzed the behavior of Bad,Bax,caspase-3 and mitochondrial transmembrane potential,we observed that cells in the h-C2C12 group were resistant to H2O2 induction of *** propose seriallycultured C2C12 cells as a refractory model to H2O2-induced *** addition,the data obtained in thiswork suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.

关键词： over-subculturing changes aged muscle cultures mitochondrial DNA

Purification,characterization and antiproliferative activity of L-asparaginase from Aspergillus oryzae CCT 3940 with no glutaminase activity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 785-794页

作者： Fernanda Furlan goncalves Dias ana Lucia Tasca Gois Ruiz Adriana Della Torre Helia Harumi Sato Department of Food Science School of Food EngineeringUniversity of Campinas Division of Pharmacology and Toxicology Multidisciplinary Center for ChemicalBiological and Agricultural ResearchUniversity of Campinas

Objective:To explore the anti-proliferative activity of purified L-asparaginase from Aspergillus oryzae CCT 3940(***).Methods:L-asparaginase was produced by submerged fermentation and purified to electrophoresis homogeneity by ionic exchanged chromatography in a fast protein liquid chromatographic *** purified enzyme was characterized and used for the antiproliferative assay against nine tumor cell lines and one non-tumor cell ***:The free glutaminase L-asparaginase was purified 28.6 fold.L-asparaginase showed high stability under physiological condition,remaining stable in the p H range7.0–8.0 after 1 h incubation at temperature range 30–45℃.The Km and Vmax values of purified L-asparaginase were estimated as 0.66 mmol/L and 313 IU/mL,*** purified enzyme could inhibit the growth of a broad range of human tumor cell lines at the concentrations ***,the enzyme from *** CCT 3940 could inhibit tumor growth of leukemia cell line(K562) with a total growth inhibition value of(3.2 ± 2.5) IU/mL and did not inhibit the non-carcinogenic human cell line growth at the concentrations ***:The sensitivity of the cells lines to purified L-asparaginase from *** CCT 3940 appeared to be concentration dependent affording a more significant decrease in cell growth than that observed for the commercial L-asparaginase from Escherichia *** L-asparaginase from *** CCT 3940 has a high potential for pharmaceutical exploitation in the treatment of leukemia.

关键词： L-asparaginase Aspergillus oryzae Purification Anti-tumoral activity

The peptide fraction of Bothrops jararaca snake venom induces toxicological effects on the male reproductive system after local envenomation in mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

BIOCELL 2023年第2期47卷 289-295页

作者： CARLOS ALBERTO-SILVA ana carolina DE ARAUJO RODRIGO SIMÃO BONFIM JOYCE MEIRE GILIO Natural and Humanities Sciences Center Experimental Morphophysiology LaboratoryFederal University of ABC(UFABC)São Bernardo do Campo09606-070Brazil Departamento de Biofísica Universidade Federal de São PauloSão Paulo04023-062Brazil

Bothrops envenomation is complex and provokes prominent local tissue damage and systemic disturbances,but little is known about their effects on the male reproductive *** intratesticular injection,the bioactive peptide fraction(Bj-PF)obtained from Bothrops jararaca snake venom changes the structure of different stages of the seminiferous epithelium cycle in adult *** the first time,we investigated whether local envenomation of Bj-PF induces toxicological effects on the male reproductive system,particularly on the seminiferous epithelium and Sertoli *** adult mice were treated with 0.24 ***^(-1) by intramuscular(i.m.)injection for 24 *** testes samples were collected for morphological and morphometric *** toxicological effects of Bj-PF were also analyzed on mitochondrial metabolism and nitrite(NO2)production in 15P-1 Sertoli cell ***-PF changed the structure and function of the seminiferous epithelium,particularly the disruption of the epithelium and the presence of degenerated germ cells in the adluminal compartment,but there were no alterations in the basal ***-PF increased the thickness of the seminiferous epithelium and decreased the lumen diameter of the *** histological assessment of the degree of tubule degeneration revealed that Bj-PF also increased the number of hypospermatogenic tubules compared to ***-PF reduced NO2 levels in 15P-1 Sertoli cells without changing the mitochondrial ***,the fact that Bj-PF alters the structure and function of the seminiferous epithelium suggests that bioactive peptides found in *** snake venom can have toxicological effects on the reproductive systems of affected male mice,providing new insight into the biological characteristics of snake venom and therapeutic strategies for envenomation inflammation.

关键词： Snake venom Bothrops jararaca Reproductive toxicology Seminiferous epithelium