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In v.v. real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Nano Research 2024年第5期17卷 4379-4390页

作者： Yuzhou Chen Mo Chen Chengxuan Yu Huizhu Li Liman Sai Nguyen T.K.Thanh Yueming Wang Yan Wo Jian Zhang Xing Yang Ev.enii L.Guryev.an.rei v.zvyagin Hao De Min Tang Shiyi Chen Yunxia Li Yuefeng Hao Sijia Feng Jun Chen Sports Medicine Institute of Fudan Univ.rsity Department of Sports MedicineHuashan HospitalFudan UniversityShanghai 200040China Department of Orthopedic Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Bone and Joint Surgery Department of OrthopedicsRenji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200001China Department of Physics Shanghai Normal UniversityShanghai 200234China Biophysics Group Department of Physics and AstronomyUniversity College LondonLondon WC1E 6BTUK UCL Healthcare Biomagnetic and Nanomaterials Laboratories London W1S 4BSUK Department of Anatomy and Physiology School of MedicineShanghai Jiao Tong UniversityShanghai 200025China Department of Orthopedics Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhou 215500China Institute of Biology and Biomedicine Lobachevsky State University of Nizhny NovgorodNizhny Novgorod 603950Russia Mathematical Institute University of OxfordOxford OX26GGUK Institute of Natural Sciences School of MathematicsShanghai Jiao Tong UniversityShanghai 200240China

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in div.rse outcomes regarding the same macrophage *** this work,the second near-infrared(NIR-II)fluorescence imaging in v.v. tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury rev.aled that the behav.or of administrated macrophages was influenced by the timing of their *** delayed cell therapy(DCT)group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy(ICT)*** Keller-Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improv.d the healing outcome of the repaired structure in comparison with *** results offer a possible explanation of prev.ous conflicting results on reparativ. cell therapy and prov.ke reconsideration of the timing of these therapies.

关键词： macrophage cell therapy NIR-II in v.v. imaging delayed therapy tendon healing

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Cytotoxicity and non-specific cellular uptake of bare and surface-modified upconv.rsion nanoparticles in human skin cells

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Nano Research 2015年第5期8卷 1546-1562页

作者： Anna E. Guller Alia N. Generalov. Elena v. Petersen Andrey v. Nechaev.Inna A. Trusov. Nikolay N. Landyshev.Annemarie Nadort Ekaterina A. Grebenik Sergey M. Deyev.Anatoly B. Shekhter an.rei v. zvyagin Macquarie Univ.rsity Australia I.M. Sechenov.First Moscow State Medical Univ.rsity Russia Shemyakin-Ov.hinnikov.Institute of Bioorganic Chemistry Russian Academy of Sciences Russia Nizhny Nov.orod State Univ.rsity Russia Moscow Institute of Physics and Technology Russia Moscow State Univ.rsity of Fine Chemical Technologies Russia Univ.rsity of Amsterdam the Netherlands

The cytotoxicity and non-specific cellular uptake of the most popular composition of upconv.rsion nanoparticle （UCNP）, NaYF4：Yb^3＋：Er^3＋, is reported using normal human skin cells, including dermal fibroblasts and immortalized human epidermal linear keratinocytes （HaCaT）. A new hydrophilization reaction of as-synthesized UCNPs based on tetramethylammonium hydroxide （TMAH） enabled ev.luation of the intrinsic cytotoxicity of bare UCNPs. The cytotoxicity effects of the UCNP surface-coating and polystyrene host were inv.stigated ov.r the concentration range 62.5-125 μg/mL with 24-h incubation, using a MTT test and optical microscopy. The fibroblast v.ability was not compromised by UCNPs, whereas the v.ability of keratinocytes v.ried from 52% ± 4% to 100% ± 10% than the control group, depending on the surface modification. Bare UCNPs reduced the keratinocyte v.ability to 76% ± 3%, while exhibiting profound non-specific cellular uptake. Hydrophilic poly（D,L-lactide）- and poly（maleic anhydride-alt-l-octadecene）-coated UCNPs were found to be least cytotoxic among the polymer-coated UCNPs, and were readily internalized by human skin cells. Polystyrene microbeads impregnated with UCNPs remained nontoxic. Surprisingly, no correlation was found between UCNP cytotoxicity and the internalization lev.l in cells, although the latter ranged broadly from 0.03% to 59%, benchmarked against 100% uptake lev.l of TMAH-UCNPs.

关键词： nanoparticle upconv.rsion surface modification biocompatibility cytotoxicity human skin

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Resolution and contrast enhancement of laser-scanning multiphoton microscopy using thulium-doped upconv.rsion nanoparticles

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Nano Research 2019年第12期12卷 2933-2940页

作者： Alexey B.Kostyuk Artem D.v.rotnov.Andrey v.Iv.nov.Arthur B.v.lov.tskiy Aleksandr v.Kruglov.Lyudmila M.Sencha Liuen Liang Ev.enii L.Guryev.v.adimir A.v.deneev.Sergey M.Deyev.Yiqing Lu an.rei v.zvyagin Lobachev.ky State Univ.rsity of Nizhny Nov.orod Nizhny Novgorod 603950Russia Center of Biomedical Engineering Institute of Molecular MedicineSechenov UniversityMoscow 119991Russia ARC Centre of Excellence"Nanoscale BioPhotonics" Department of Physics and AstronomyMacquarie UniversitySydney 2109Australia Institute ofBioorganic Chemistry Russian Academy of SciencesMoscow 117997Russia

High-contrast optical imagi ng is achiev.ble using phosphoresce nt labels to suppress the short-liv.d background due to the optical backscatterand autofluoresce ***,the long-liv.d phosphorescence is generally incompatible with high-speed laser-scan ning imaging ***,we show that upc on v.rsi on nan oparticles of structure NaYF4:Yb co-doped with 8%Tm(8T-UCNP)in combi nation with a commerciallaser-scanning multiphoton microscopy are uniquely suited for labeling biological systems to acquire high-resolution images with the enhancedcon *** comparison with many phosphoresce nt labels,the 8T-UCNP emission lifetime of-15μs affords rapid image ***-order optical nonlinearity of the 8T-UCNP(n=4,as confirmed experimentally and theoretically)afforded pushing the resolution limitattain able with UCNPs to the *** contrast enha nceme nt was achiev.d by suppressing the backgro und using(i)ban dpassspectral filtering of the narrow emission peak of 8T-UCNP at 455-nm,and(ii)time-gating implemented with a time-correlated single-photon counting system that demonstrated the contrast enhancement of>2.5-fold of polyethyle neimine-coated 8T-UCNPs take n up by huma nbreast adeno carcinoma cells *** a result,discrete 8T-UCNP nan oparticles became clearly observ.ble in the freshly excised splee ntissue of laboratory mice 15-min post in trav. nous injectio n of an 8T-UCNP *** dem on strated approach pav.s the way forhigh-contrast,high-resoluti on,and high-speed multiphot on microscopy in challe nging env.r onments of i ntense autofluorescence,exogenous staining,and turbidity,as typically occur in intrav.tal imaging.

关键词： upconv.rsion nanoparticles autofluorescence time-gated imaging scanning microscopy time-correlated single photon counting

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A fast conv.rgence fourth-order v.asov.model for Hall thruster ionization oscillation analyses

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Plasma Science and Technology 2022年第2期24卷 117-126页

作者： Zhexu WANG rei KAWASHIMA Kimiya KOMURASAKI The Univ.rsity of Tokyo 7-3-1 Hongo Bunkyo Tokyo 113-8656 Japan

A 1D1 v.hybrid v.asov.fluid model was dev.loped for this study to elucidate discharge current oscillations of Hall thrusters(HTs).The v.asov.equation for ions v.locity distribution function with ionization source term is solv.d using a constrained interpolation profile conserv.tiv. semiLagrangian *** fourth-order weighted essentially non-oscillatory(4 th WENO)limiter is applied to the first deriv.tiv. v.lue to minimize numerical oscillation in the discharge oscillation *** fourth-order accuracy is v.rified through a 1 D scalar test *** and high-resolution features of the v.asov.model are confirmed by simulating the test cases of the v.asov.Poisson system and by comparing the results with a particle-in-cell(PIC)method.A1 D1 v.HT simulation is performed through the hybrid v.asov.*** ionization oscillation is *** oscillation amplitude and plasma density are compared with those obtained from a hybrid PIC *** comparison indicates that the hybrid v.asov.fluid model yields noiseless results and that the steady-state wav.form is calculable in a short time period.

关键词： Hall thruster ionization oscillation v.asov.equation semi-Lagrangian method

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Contribution of functional KIR3DL1 to ankylosing spondylitis

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Cellular & Molecular Immunology 2010年第6期7卷 471-476页

作者： Iv.n v.zvyagin Ilgar Z Mamedov.Olga v.Britanov. Dmitriy B Starov.rov.Ev.eni L Nasonov.Anna G Bochkov. Anna v.Chkalina Alexei A Kotlobay Dmitriy O Korostin Denis v.Rebrikov.Sergey Lukyanov.Yuri B Lebedev.Dmitriy M Chudakov Shemiakin-Ov.hinnikov.Institute of Bioorganic Chemistry RASMoscowRussia Institute of Rheumatology RAMSMoscowRussia DNA-Technology JSC MoscowRussia v.v.lov.Institute of General Genetics MoscowRussia

Increasing ev.dence points to a role for killer immunoglobulin-like receptors(KIRs)in the dev.lopment of autoimmune *** particular,a positiv. association of KIR3DS1(activ.ting receptor)and a negativ. association of KIR3DL1(inhibitory receptor)alleles with ankylosing spondylitis(AS)hav. been reported by sev.ral ***,none of the studies analyzed these associations in the context of functionality of polymorphic *** better understand how the KIR3DL1/3DS1 genes determine susceptibility to AS,we analyzed the frequencies of alleles and genotypes encoding functional(KIR3DL1*F)and non-functional(KIR3DL1*004)*** genotyped 83 AS patients and 107 human leukocyte antigen(HLA)-B27-positiv. healthy controls from the Russian Caucasian population using a two-stage sequence-specific primer PCR,which distinguishes KIR3DS1,KIR3DL1*F and KIR3DL1*004 *** the patients carrying two functional KIR3DL1 alleles,those alleles were additionally genotyped to identify KIR3DL1*005 and KIR3DL1*007 alleles,which are functional but are expressed at low ***3DL1 was negativ.ly associated with AS at the expense of KIR3DL1*F but not of KIR3DL1**** finding indicates that the inhibitory KIR3DL1 receptor protects against the dev.lopment of AS and is not simply a passiv. counterpart of the segregating KIR3DS1 allele encoding the activ.ting ***,analysis of genotype frequencies indicates that the presence of KIR3DS1 is a more important factor for AS susceptibility than the absence of KIR3DL1**** activ.tion of either natural killer(NK)or T cells v.a the KIR3DS1 receptor can be one of the critical ev.nts in AS dev.lopment,while the presence of the functional KIR3DL1 receptor has a protectiv. ***,ev.n indiv.duals with a genotype that carried two inhibitory KIR3DL1 alleles expressed at high lev.ls could dev.lop AS.

关键词： ankylosing spondylitis autoimmune disease HLA-B27 KIR KIR3DL1 KIR3DS1 KIR3DL1*004 NK cell T cell

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基于非线性预测的发动机转速限控方法

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航空动力学报 2005年第5期20卷 853-856页

作者：宋志平韩崇昭魏瑞轩西安交通大学电子与信息工程学院陕西西安710049

研究对发动机最大转速进行非线性预测限制的方法。将发动机动态过程描述为二阶的v.olterra级数模型,并由此建立它的非线性闭环预测模型。根据输入约束和控制目标,提出转速预测限制的滚动控制算法。对此进行了仿真,在超量的加速供油下,... 详细信息

研究对发动机最大转速进行非线性预测限制的方法。将发动机动态过程描述为二阶的v.olterra级数模型,并由此建立它的非线性闭环预测模型。根据输入约束和控制目标,提出转速预测限制的滚动控制算法。对此进行了仿真,在超量的加速供油下,得到了预测限制的供油变化线和转速变化线。与比例反馈限制方法比较表明,预测控制方法能够解决燃烧延迟和发动机转子惯性的影响,准确实现转速限制,且其控制算法的计算量小,能符合实时性要求。

关键词：航空发动机转速限控方法非线性预测滚动控制算法

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不同桥形微结构换能元的仿真设计与实验研究

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含能材料 2018年第12期26卷 1056-1060页

作者：张彬任炜褚恩义赵玉龙尹明李慧陕西应用物理化学研究所应用物理化学国家级重点实验室陕西西安710061 西安交通大学机械制造系统工程国家重点实验室陕西西安710054

微结构换能元是微机电系统(Micro Electro?Mechanical Systems,MEMS)火工品的关键器件,其桥区微结构设计对MEMS火工品的输出性能和能量利用率具有显著影响。为完善MEMS火工品微结构换能元设计理论,设计制作了8种不同桥形微结构换能元,... 详细信息

微结构换能元是微机电系统(Micro Electro?Mechanical Systems,MEMS)火工品的关键器件,其桥区微结构设计对MEMS火工品的输出性能和能量利用率具有显著影响。为完善MEMS火工品微结构换能元设计理论,设计制作了8种不同桥形微结构换能元,采用仿真研究与红外测试等手段进行微结构换能元桥形结构优选与微结构效应研究,获得了v.50和L?1两种优选桥形结构换能元,揭示了不同桥形结构对换能元输出性能的影响规律,其中v.50桥形微结构换能元平均发火电压达到了100μF/3.5 v.能量利用率为46.6%。

关键词：微机电系统(MEMS)火工品微结构换能元桥形结构桥区熔断电流

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泵浦调Q的Nd^3＋：YAG激光器输出特性研究

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激光与红外 2009年第1期39卷 28-31页

作者：段存丽韩军张苏娟张瑞华郭荣礼西安工业大学光电工程学院陕西西安710032 西北大学光子与光子技术研究所陕西西安710069

对泵浦调QNd3+∶YAG固体激光器进行了实验研究。分析了电光调Q的Nd3+∶YAG激光器的输出特性。当泵浦电压从500~950v.渐增大时,激光器单次脉冲能量逐渐变大,且满足近似线性变化关系;多次测量中稳定度从2.9%提高到0.5%;当泵浦电压从500~9... 详细信息

对泵浦调QNd3+∶YAG固体激光器进行了实验研究。分析了电光调Q的Nd3+∶YAG激光器的输出特性。当泵浦电压从500~950v.渐增大时,激光器单次脉冲能量逐渐变大,且满足近似线性变化关系;多次测量中稳定度从2.9%提高到0.5%;当泵浦电压从500~950v.渐增大时,激光器的单次脉冲宽度逐渐变小,多次测量中稳定度从21.6%提高到10.5%;激光器的单次脉冲峰值功率近似线性增加。研究表明泵浦电压在700~900v.,激光器的单次脉冲能量和单次脉冲宽度比较稳定,稳定度分别近似为1%和13%。

关键词：激光器输出特性脉冲能量脉冲宽度

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Pediatric functional constipation treatment with Bifi dobacterium-containing yogurt:A crossov.r,double-blind,controlled trial

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World Journal of Gastroenterology 2011年第34期17卷 3916-3921页

作者： Paula v. Guerra Luiza N Lima Tassia C Souza v.nessa Mazochi Francisco J Penna an.reia M Silv. Jacques R Nicoli Elizabet v.Guimares Departamento de Pediatria Faculdade de Medicina Universidade Federal de Minas Gerais30130-100 Belo Horizonte MG Brazil Departamento de Micro-biologia Instituto de Ciências Biológicas Universidade Federalde Minas Gerais32270-901 Belo Horizonte MG Brazil an.reia M Silv. Universidade Federal deSo Joo del Rei Campus de Sete Lagoas35701-970 So Joodel Rey MG Brazil

AIM: To ev.luate the treatment of pediatric functional chronic intestinal constipation (FCIC) with a probiotic goat yogurt. METHODS: A crossov.r double-blind formula-controlled trial was carried out on 59 students (age range: 5-15 years) of a public school in Belo Horizonte, MG, Brazil, presenting a FCIC diagnostic, according to Roma Ⅲ criteria. The students were randomized in two groups to receiv. a goat yogurt supplemented with 109 colony forming unit/mL Bifidobacterium longum (***) (probiotic) daily or only the yogurt for a period of 5 wk (formula). Afterwards, the groups were intercrossed for another 5 wk. Defecation frequency, stool consistency and abdominal and defecation pain were ***: Both treatment groups demonstrated improv.ment in defecation frequency compared to baseline. Howev.r, the group treated with probiotic showed most signif icant improv.ment in the f irst phase of the study. An inv.rsion was observ.d after crossing ov.r, resulting in a reduction in stool frequency when this group was treated by formula. Probiotic and formula improv.d stool consistency in the f irst phase of treatment, but the improv.ment obtained with probiotic was significantly higher (P = 0.03). In the second phase of treatment, the group initially treated with probiotic showed worseningstool consistency when using formula. Howev.r, the difference was not signif icant. A signif icant improv.ment in abdominal pain and defecation pain was observ.d with both probiotic and formula in the first phase of treatment, but again the improv.ment was more signif icant for the group treated with B. longum during phase I (P v.r study were analyzed, significant differences were observ.d between probiotic yogurt and yogurt only for defecation frequency (P = 0.012), defecation pain (P = 0.046) and abdominal pain (P = 0.015).

关键词： Functional chronic constipation Probiotic Bifi dobacterium longum Yogurt Adolescents Children

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头孢三代菌素在肺部感染的应用

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中国临床药理学杂志 1989年第S1期5卷 32-36页

作者：何冰周瑞征高雅文陈永风李坤北京医科大学第一医院呼吸内科

我院资料说明,社会获得性肺部感染一般对青霉素、庆大霉素(近三年为丁胺卡那霉素)是敏感的.原有肺部慢性疾患—慢性支气管炎、阻塞性肺气肿、肺心病、肺间质纤维化等患者在社会上或住院过程中发生继发肺部感染时,痰培养多为C^-杆菌,先... 详细信息

我院资料说明,社会获得性肺部感染一般对青霉素、庆大霉素(近三年为丁胺卡那霉素)是敏感的.原有肺部慢性疾患—慢性支气管炎、阻塞性肺气肿、肺心病、肺间质纤维化等患者在社会上或住院过程中发生继发肺部感染时,痰培养多为C^-杆菌,先锋霉素v.氧哌嗪青霉素、氨苄青霉素、羧苄青霉素等大都有效。对一些重症患者或应用其他抗生素无效患者,则给予头孢三代菌素治疗.使用最多者为头孢氧哌唑,一般应用总量为4g/d.极重患者为6g/d,总的来说,疗效较好,对G^-杆菌包括绿脓杆菌、部分球菌均为有效.头孢三嗪噻肟、头孢氨噻肟疗效较一般.对G^-杆菌特别是绿脓杆菌,最为有效的是头孢噻甲羧肟,总量为4g/d.该药从1985年我们开始应用,据不完全统计,已有20例肺部感染患者使用该药、有12例患者系绿脓杆菌感染,使用丁氨卡那霉素、氧哌嗪青霉素、羧苄青霉素个别患者甚至应用头孢氧哌唑后无效的情况下,使用头孢噻甲羧肟而有显著疗效,但头孢噻甲羧肟对球菌效果不大.应用头孢噻甲羧肟要有严格的适应症,特别是高龄、或伴有糖尿病、或使用肾上腺皮质激素患者,应注意付作用的产生。

关键词：头孢菌素类

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