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Chinese Chemical Letters 2012年第1期23卷 97-100页

作者： jiao xia Sun Yuan Liang Wang Shu Hua Dou Key Lab of Biorheological Science and Technology Ministry of EducationResearch Center of BioinspiredMaterial Science and EngineeringCollege of BioengineeringChongqing UniversityChongqing 400030China

A new positively thermo-sensitive hydrogel was designed and synthesized by a condensation polymerization reaction of ethylenediaminetetraacetic dianhydride（EDTAD） and piperazine（PA） to give poly（ethylenediaminetetraacetic dianhydride-copiperazine）（PEP）.The obtained polymers＇ structure was characterized by FTIR and ^（13）C *** backbone of the polymer linked by amide bond and abundant of carboxyl groups as pendant group could form strong intermolecular and intramolecular hydrogen bond at lower temperature and dissociate at higher temperature,resulting in the polymer with *** aqueous solution of PEP at lower temperature（〈20℃） showed micro-gel formation and transformed to transparent solution at higher temperature（〉40℃）.Transition temperature shifted to higher value with the increasing of *** hydrogel exhibited reversible phase transition and the transmittance change was not weakened by multiple temperature changes.

关键词： Positively thermo-sensitive hydrogel Degradation Ethylenediaminetetraacetic dianhydride Piperazine

Oleuropein alleviates sepsis-induced acute lung injury via the AMPK/Nrf-2/HO-1 signaling

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Asian Pacific Journal of Tropical Biomedicine 2024年第5期14卷 187-198页

作者： Shan-Hu Wang Yang-Yang Wu xiao-jiao xia Department of Pediatrics The Second Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang 325027China

Objective:To explore the effect of oleuropein on sepsis-induced acute lung injury(ALI)in vitro and in vivo and investigate the underlying ***:In an lipopolysaccharide(LPS)-mediated cell model of sepsis-induced ALI and a cecal ligation and puncture-induced mouse model of septic ALI,CCK-8 assay and flow cytometry analysis were used to detect cell activity and *** and relevant assay kits were used to measure the levels of inflammatory cytokines and oxidative stress,*** blot was applied to determine the expression of apoptosis-and AMP-activated protein kinase(AMPK)/nuclear factor erythroid 2-related factor-2(Nrf-2)/heme oxygenase-1(HO-1)signaling-associated ***-1 staining,adenosine triphosphate(ATP)assay kit,and MitoSOX Red assays were performed to detect mitochondrial membrane potential,ATP content,and mitochondrial ROS formation,***,lung injury was evaluated by measuring lung morphological alternations,lung wet-to-dry ratio,myeloperoxidase content,and total protein ***:Oleuropein reduced inflammatory reaction,oxidative damage,and apoptosis,and ameliorated mitochondrial dysfunction in LPS-exposed BEAS-2B cells and mice with septic ***,oleuropein activated the AMPK/Nrf-2/HO-1 signaling ***,these effects of oleuropein were abrogated by an AMPK inhibitor compound ***:Oleuropein can protect against sepsis-induced ALI in vitro and in vivo by activating the AMPK/Nrf-2/HO-1 signaling,which might be a potential therapeutic agent for the treatment of sepsis-induced ALI.

关键词： AMPK/Nrf-2/HO-1 signaling Inflammatory response Lung damage Mitochondrial dysfunction Oleuropein Oxidative stress Sepsis

Design,synthesis and characterization of a novel pH-sensitive hydrogel

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Chinese Chemical Letters 2008年第12期19卷 1475-1478页

作者： jiao xia Sun Yan Feng Luo Hui Peng Zhi Wei Han College of Chemistry ＆ Chemical Engineering Chongqing University Chongqing 400030 China College of Bioengineering Chongqing University Chongqing 400030 China

A novel degradable pH-sensitive hydrogel with pendent carboxyl groups was designed and synthesized from ethylenediaminetetraacetic dianhydride （EDTAh） and butanediamine （BDA） with dicyclohexylcarbodiimide （DCC） as a condensating agent and BDA as a crosslinking agent. The obtained polymers were characterized by ^13C NMR, ^1H NMR and FTIR. The swelling experiments of the hydrogel in pH 3, 7, and 12 media indicated much higher swelling ratio in pH 12 media than in pH 3 and pH 7 media, exhibiting sound pH sensitivity. The pH sensitivity of this type of hydrogel may be regulated through controlling the type and the dose of the crosslinking agent.

关键词： pH-sensitive hydrogel Ethylenediaminetetraacetic dianhydride Butanediamine

GLYX-13 pretreatment ameliorates long-term isoflurane exposure-induced cognitive impairment in mice

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Neural Regeneration Research 2020年第1期15卷 128-135页

作者： Huan Liu xiang-Dan Gong Xin Zhao Yue Qian xiao-Ping Gu Tian-jiao xia Department of Anesthesiology The Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical SchoolNanjingJiangsu ProvinceChina Jiangsu Key Laboratory of Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina

Accumulating evidence indicates that inhalation anesthetics induce or increase the risk of cognitive impairment. GLYX-13（rapastinel） acts on the glycine site of N-methyl-D-aspartate receptors（NMDARs） and has been shown to enhance hippocampus-dependent learning and memory function. However, the mechanisms by which GLYX-13 affects learning and memory function are still unclear. In this study, we investigated these mechanisms in a mouse model of long-term anesthesia exposure. Mice were intravenously administered 1 mg/kg GLYX-13 at 2 hours before isoflurane exposure（1.5% for 6 hours）. Cognitive function was assessed using the contextual fear conditioning test and the novel object recognition test. The mRNA expression and phosphorylated protein levels of NMDAR pathway components, N-methyl-D-aspartate receptor subunit 2B（NR2B）-Ca2+/calmodulin dependent protein kinase II（CaMKII）-cyclic adenosine monophosphate response element binding protein（CREB）, in the hippocampus were evaluated by quantitative RT-PCR and western blot assay. Pretreatment with GLYX-13 ameliorated isoflurane exposure-induced cognitive impairment and restored NR2B, CaMKII and CREB mRNA and phosphorylated protein levels. Intracerebroventricular injection of KN93, a selective CaMKII inhibitor, significantly diminished the effect of GLYX-13 on cognitive function and NR2B, CaMKII and CREB levels in the hippocampus. Taken together, our findings suggest that GLYX-13 pretreatment alleviates isoflurane-induced cognitive dysfunction by protecting against perturbation of the NR2B/CaMKII/CREB signaling pathway in the hippocampus. Therefore, GLYX-13 may have therapeutic potential for the treatment of anesthesia-induced cognitive dysfunction. This study was approved by the Experimental Animal Ethics Committee of Drum Tower Hospital affiliated to the Medical College of Nanjing University, China（approval No. 20171102） on November 20, 2017.

关键词： Ca2+/calmodulin-dependent protein kinase II cognitive impairment contextual fear conditioning cyclic adenosine monophosphate response element binding protein GLYX-13 isoflurane N-methyl-D-aspartate receptor novel object recognition rapastinel

Sevoflurane plays a reduced role in cognitive impairment compared with isoflurane: limited effect on fear memory retention

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Neural Regeneration Research 2020年第1期15卷 96-102页

作者： Ying Du xiang-Dan Gong Xin Fang Fang Xing Tian-jiao xia xiao-Ping Gu Department of Anesthesiology Affiliated Drum Tower Hospital of Medical Department of Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory of Molecular Medicine Nanjing UniversityNanjingJiangsu ProvinceChina

Isoflurane and sevoflurane are both inhalation anesthetics,but in clinical application,sevoflurane has been considered to be less suitable for long-term anesthesia because of its catabolic compounds and potential ***,recent studies have shown that these two inhalation anesthetics are similar in hepatorenal toxicity,cost,and long-term anesthetic ***,sevoflurane possibly has less cognitive impact on young *** this study,C57BL/6 mice aged 8–10 weeks were exposed to 1.2%isoflurane or 2.4%sevoflurane for 6 *** function and memory were examined in young mice using the novel object recognition,contextual fear conditioning,and cued-fear extinction *** blot assay was performed to detect expression levels of D1 dopamine receptor,catechol-O-methyltransferase,phospho-glycogen synthase kinase-3β,and total glycogen synthase kinase-3βin the *** results show that impaired performance was not detected in mice exposed to sevoflurane during the novel object recognition *** memory impairment in the fear conditioning test was shorter in the sevoflurane group than the isoflurane ***-term sevoflurane exposure did not affect memory consolidation,while isoflurane led to memory consolidation and reduced *** of hippocampal D1 dopamine receptors and phosphorylated glycogen synthase kinase-3β/total glycogen synthase kinase-3βand upregulation of catechol-O-methyltransferase may be associated with differing memory performance after exposure to isoflurane or *** results confirm that sevoflurane has less effect on cognitive impairment than isoflurane,which may be related to expression of D1 dopamine receptors and catechol-O-methyltransferase and phosphorylation of glycogen synthase kinase-3βin the *** study was approved by the Institutional Animal Care and Use Committee,Nanjing University,China on November 20,2017(approval No.20171102).

关键词： catechol-O-methyltransferase cognitive impairment D1 dopamine receptors fear memory glycogen synthase kinase-3β isoflurane neurotoxicology sevoflurane

Spontaneous firing properties of rat medial vestibular nucleus neurons in brain slices by infrared visual patch clamp technique

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Frontiers of Medicine 2008年第3期2卷 264-268页

作者： jiao xia Weijia KONG Yun ZHU Yan ZHOU Yu ZHANG Changkai GUO Department of Otorhinolaryngology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Domestic application of infrared patch clamp techniques on brain slices is *** key of the tech-nique is to prepare high-quality brain *** present paper describes the preparation procedure of brainstem slices and the spontaneous firing properties of rat medial vestibular nucleus(MVN)*** infrared differ-ential interference contrast technique,neurons of rat MVN were visualized directly at the depth of 50–100 mm underneath the surface of *** activities of MVN neurons were recorded by the whole-cell patch clamp technique in artificial cerebrospinal fluid(ACSF)and low Ca^(2+)-high Mg^(2+) *** firing mode was more irregular and depressive in low Ca^(2+)-high Mg2+fluid than in *** to the averaged waveform of action potentials,cells were classified as the neurons with mono-phasic after-hyperpolarization potential(AHP),and the neurons with biphasic *** resting membrane potential(RMP),input resistance(Rin)and membrane capacitance(Cm)of neurons were recorded and com-pared between *** infrared videomicroscopy,patch clamp recordings could be made under direct obser-vation in freshly prepared brainstem *** discharge activities of MVN neurons were spontaneous and the fir-ing mode was modulated by extracellular calcium *** basic membrane properties of two types of neurons were not significantly different,while the differ-ences in waveform might play a role in the segregation between tonic and kinetic cells.

关键词： vestibular nucleus,medial brainstem slices infrared videomicroscopy whole-cell recording patch-clamp techniques spontaneous firing

Evolutionarily conserved boule and dazl identify germ cells of Coilia nasus

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Aquaculture and Fisheries 2023年第3期8卷 244-251页

作者： xiao Chen Peng Song jiao xia Jing Guo Yonghai Shi Ying Zhong Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China Shanghai Fisheries Research Institute Shanghai Fisheries Technology Extension StationShanghai200433China

Coilia nasus is an endangered fish species in the Yangtze River,and there is urgent need to know the genes related to its reproduction and *** DAZ family including boule,daz and dazl,plays an important role in germ cells *** this study,the cDNA sequence of boule(Cnbol)and dazl(Cndazl)genes were cloned and their expression patterns were characterized in Coilia ***-PCR showed that the expression of Cnbol and Cndazl mRNAs was restricted in adult *** section in situ hybridization indicated that the temporospatial expression patterns of Cnbol and Cndazl were significantly *** the testis,Cnbol was mainly detected in spermatocytes and spermatids,while Cndazl was prominently expressed in s *** the ovary,Cnbol and Cndazl were highly expressed in the early stages of ***,Cndazl was also concentrated in perinuclear speckle and then localized to the Balbiani body in late stages of *** addition,Cnbol and Cndazl 3′UTR can specifically label medaka Primordial Germ Cells(PGCs).According to our results,Cnbol and Cndazl are marker genes of germ cells and may play a vital role in the development and differentiation of germ cells in Coilia nasus.

关键词： Coilia nasus Germ cells boule dazl PGCs

Photoacoustic treatment mitigates cognitive dysfunction in a model of sleep-wake rhythm disturbance

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Neural Regeneration Research 2020年第6期15卷 1094-1101页

作者： Fang Xing Xin Fang xiang-Dan Gong Xin Zhao Ying Du Zheng-Liang Ma xiao-Ping Gu Tian-jiao xia Department of Anesthesiology Affiliated Drum Tower Hospital of Medical Department of Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory of Molecular Medicine Nanjing UniversityNanjingJiangsu ProvinceChina

Sleep-wake rhythm disturbances,which are characterized by abnormal sleep timing or duration,are associated with cognitive *** treatments including light and sound stimulation have been found to be effective in modulating sleep patterns and improving cognitive behavior in abnormal sleep-wake pattern *** this study,we examined whether light and sound interventions could reduce sleep-wake pattern disturbances and memory deficits in a sleep rhythm disturbance *** established a model of sleep rhythm disturbance in C57 BL/6 J mice via a sleep deprivation method involving manual cage tapping,cage jostling,and nest *** used a Mini Mitter radio transmitter device to monitor motor activity in the mice and fear conditioning tests to assess cognitive *** results indicated that an intervention in which the mice were exposed to blue light(40-Hz flickering frequency)for 1 hour during their subjective daytime significantly improved the 24-hour-acrophase shift and reduced the degree of memory deficit induced by sleep ***,interventions in which the mice were exposed to a 40-Hz blue light at offset time or subjective night time points,as well as 2 Hz-blue light at 3 intervention time points(subjective day time,subjective night time,and offset time points),had no positive effects on circadian rhythm shift or memory ***,a 2000-Hz sound intervention during subjective day time attenuated the24-hour-acrophase shift and memory decline,while 440-Hz and 4000-Hz sounds had no effect on circadian ***,these results demonstrate that photoacoustic treatment effectively corrected abnormal sleep-wake patterns and cognitive dysfunction associated with sleep-deprivation-induced disturbances in sleep-wake *** animal experiments were approved by the Experimental Animal Ethics Committee of Drum Tower Hospital Affiliated to the Medical College of Nanjing University,China(approval No.20171102)on N

关键词： circadian rhythm cognitive impairment fear conditioning light intervention photoacoustic treatment rhythm disturbance rhythm shift sleep deprivation sleep-wake rhythm sound intervention

The gas sensing properties of TiO_2 nanotubes synthesized by hydrothermal method

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Chinese Chemical Letters 2011年第5期22卷 603-606页

作者： Yan Li Wang Shun Tan Jia Wang Zhi Jin Tan Qiu xia Wu Zheng jiao Ming Hong Wu Institute of Nanochemistry and Nanobiology School of Environmental and Chemical Engineering Shanghai University Shanghai 200444 China

In this paper,the TiO_2 nanotubes were synthesized by hydrothermal method using a 10 mol/L NaOH aqueous solution at 150℃. The structure of prepared materials was characterized by X-ray diffraction（XRD）,transmission electron microscopy（TEM）. scanning electron microscope（SEM） and Brunauer-Emmett-Teller（BET）.The prepared TiO_2 nanotubes were used to prepare thick film gas sensors and the gas sensing properties to various gases were *** show the prepared TiO_2 nanotube gas sensors responses to ethanol under dry condition at 450℃.This could be attributed to the fact that it had high porous morphology and a higher pore volume,which can promote the diffusion of ethanol deep inside the films and improve the sensor response. Moreover,the gas sensor made with nanotubes exhibit high selective response towards ethanol gas compared with H_2,CO,acetone.

关键词： TiO_2 nanotubes Hydrothermal method Gas sensing

Discussion　on　the　physics　phenomena　and　the　mechanism　of　forming　weld　joint　in　the　process　of　copper－stainless　steel　friction　welding

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China Welding 1995年第1期4卷 19-27页

作者： Shi Kai(Xi’an Petroleum Institute)Wang Shiyan Qiu Fengxiang and xia Wei(Xi’an jiao Tong University)

The physics phenomena of copper and stainless steel in the process of forming friction welding joint are recorded and observed by the interrupted test *** test shows that the butt friction welding process of copper and stainless steel bar can be divided into three stages approximately: the adhesion at the beginning friction stage, the friction inside the copper and the plastic ring expansion, and the friction on the bound surface of copper-stainless steel and in stainless steel. During the process,the friction surface was transformed twice.

关键词： copper-stainless steel friction welding interrupted test method