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Nano Research 2024年第1期17卷 407-415页

作者： Yumei Ma Lijie Zhang kang zhu Binze Zhang Ranran Peng Changrong Xia Ling Huang State Key Laboratory of Chemistry and Utilization of Carbon Based Energy Resources College of ChemistryXinjiang UniversityUrumqi 830046China Department of Materials Science and Engineering University of Science and Technology of ChinaHefei 230026China

Promoting the oxygen reduction reaction(ORR)is critical for commercialization of intermediate-temperature solid oxide fuel cells(IT-SOFCs),where Sr_(2)Fe_(1.5)Mo_(0.5)O_(6)−δ(SFM)is a promising cathode by working as a mixed ionic and electronic *** this work,doping of In^(3+)greatly increases the oxygen vacancy concentration and the content of adsorbed oxygen species in Sr_(2)Fe_(1.5)Mo_(0.5−x)InxO_(6−δ)(SFMInx),and thus effectively promotes the ORR *** a typical example,SFMIn_(0.1)reduces the polarization resistance(R_(p))from 0.089 to 0.046Ω∙cm^(2)at 800°C,which is superior to those doped with other metal *** addition,SFMIn0.1 increases the peak power density from 0.92 to 1.47 W∙cm^(−2)at 800°C with humidified H_(2)as the fuel,indicating that In3+doping at the Mo site can effectively improve the performance of SOFC cathode material.

关键词： solid oxide fuel cell cathode In3+doping oxygen reduction reaction(ORR) Sr_(2)Fe_(1.5)Mo_(0.5)O_(6-δ)

Knockout of miR396 genes increases seed size and yield in soybean

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Journal of Integrative Plant Biology 2024年第6期66卷 1148-1157页

作者： Hongtao Xie Fei Su Qingfeng Niu Leping Geng Xuesong Cao Minglei Song Jinsong Dong Zai Zheng Rui Guo Yang Zhang Yuanwei Deng Zhanbo Ji kang Pang Jian‐kang zhu Jianhua zhu School of Life Sciences Anhui Agricultural UniversityHefei 230036China Research Center for Biological Breeding Technology Research Institute of Frontier ScienceAnhui Agricultural UniversityHefei 230036China Bellagen Biotechnology Co.Ltd Jinan 250000China Institute of Crop Sciences/National Nanfan Research Institute Chinese Academy of Agricultural SciencesKey Laboratory of Gene Editing Technologies(Hainan)Ministry of Agriculture and Rural AffairsSanya 572025China Southern University of Science and TechnologyShenzhen 518055China Hainan Yazhou Bay Seed Laboratory Sanya 572024China

Yield improvement has long been an important task for soybean breeding in the world in order to meet the increasing demand for food and animal *** R396 genes have been shown to negatively regulate grain size in rice,but whether mi R396 family members may function in a similar manner in soybean is ***,we generated eight soybean mutants harboring different combinations of homozygous mutations in the six soybean mi R396genes through genome editing with clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease(Cas)12SF01 in the elite soybean cultivar Zhonghuang 302(ZH302).Four triple mutants(mir396aci,mir396acd,mir396adf,and mir396cdf),two quadruple mutants(mir396-abcd and mir396acfi),and two quintuple mutants(mir396abcdf and mir396bcdfi)were *** found that plants of all the mir396 mutants produced larger seeds compared to ZH302 *** tests showed that mir396adf and mir396cdf plants have significantly increased yield in growth zones with relatively high latitude which are suited for ZH302 and moderately increased yield in lower *** contrast,mir396abcdf and mir396bcdfiplants have increased plant height and decreased yield in growth zones with relatively high latitude due to lodging issues,but they are suited for low latitude growth zones with increased yield without lodging *** together,our study demonstrated that loss-of-function of mi R396 genes leads to significantly enlarged seed size and increased yield in soybean,providing valuable germplasms for breeding high-yield soybean.

关键词： Cas12SF01 CRISPR/Cas miR396 seed size soybean yield

Breeding exceptionally fragrant soybeans for soy milk with strong aroma

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Journal of Integrative Plant Biology 2024年第4期66卷 642-644页

作者： Hongtao Xie Minglei Song Xuesong Cao Qingfeng Niu Jianhua zhu Shasha Li Xin Wang Xiaomu Niu Jian-kang zhu Bellagen Biotechnology Co.Ltd. Jinan 250000China Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China School of Life Sciences Anhui Agricultural UniversityHefei 230036China Shandong Normal University Jinan 250000China

Soybean(Glycine max(L.)Merr.)is a major source of vegetable protein and oil in human diet and animal *** seeds have been extensively used in various food products and *** quality,particularly the aroma,affects cooking and eating,and ultimately influences consumer *** milk is particularly popular in China and has been gaining popularity in many other countries in the world.

关键词： exceptional soybean cooking

Simple method for transformation and gene editing in medicinal plants

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Journal of Integrative Plant Biology 2024年第1期66卷 17-19页

作者： Xuesong Cao Hongtao Xie Minglei Song Lianghui Zhao Hailiang Liu Guofu Li Jian‐kang zhu Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhen 518055China Bellagen Biotechnology Co.Ltd. Jinan 250000China Institute for Regenerative Medicine Shanghai East HospitalTongji University School of MedicineShanghai 200123China Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies Nanfan Research InstituteChinese Academy of Agricultural SciencesSanya 572024China

Gene-editing technologies have ushered in a significant advancement in plant genetics research and molecular ***,a critical challenge hindering the widespread adoption of these technologies is the efficient delivery of gene-editing *** predominant methods for introducing these tools into plants typically involve Agrobacterium tumefaciens-mediated transformation or particle bombardment(Mao et al.,2019).Unfortunately,these traditional gene delivery methods require delicate and timeconsuming tissue culture procedures and show limited success,especially in medicinal and other less studied plants.A recent breakthrough is the development of the Cut-Dip-Budding(CDB)gene delivery *** CDB system is highly effective for plants with root-suckering *** allows the delivery of transgenes and gene-editing tools into plants through hairy root induction followed by shoot regeneration from transformed hairy roots,bypassing the need for tissue culture processes(Cao et al.,2023).

关键词： transformation culture breakthrough

Targeted G-to-T base editing for generation of novel herbicide-resistance gene alleles in rice

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Journal of Integrative Plant Biology 2024年第6期66卷 1048-1051页

作者： Yifu Tian Xinbo Li Jiyong Xie Zai Zheng Rundong Shen Xuesong Cao Mugui Wang Chao Dong Jian-kang zhu Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies(Hainan) Institute of Crop Sciences and National Nanfan Research InstituteChinese Academy of Agricultural SciencesSanya 572024China Hainan Seed Industry Laboratory Sanya 572024China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China Institute of Advanced Biotechnology and School of MedicineSouthern University of Science and TechnologyShenzhen 518055China

Deaminase-based cytosine base editors(CBEs)and adenine base editors(ABEs)induce C-to-T and A-to-G transitions,respectively,enabling single-nucleotide variants(SNVs)in plants for research and crop enhancement(Li et al.,2023a).The C-to-G base editors(CGBEs)and A-to-Y base editors(AYBEs),developed by incorporating glycosylases with CBEs and ABEs,expand the repertoire of base editing products,allowing C-to-T/G and A-to-T/G transversions in plants(Li et al.,2023b,2023c;Sretenovic et a.,2021).

关键词： base alleles CBE

Efficient and multiplex gene upregulation in plants through CRISPR-Cas-mediated knockin of enhancers

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Molecular Plant 2024年第9期17卷 1472-1483页

作者： Qi Yao Rundong Shen Yang Shao Yifu Tian Peijin Han Xuening Zhang Jian-kang zhu Yuming Lu Shanghai Collaborative Innovation Center of Agri-Seeds Joint Center for Single-Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of the Chinese Academy of Sciences BeijingChina College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China institute of Advanced Biotechnology and School of Medicine Southern University of Science and TechnologyShenzhen 518055China

Geneupregulation through genome editing is important for plant research and *** insertion of short transcriptional enhancers(STEs)into gene promoters may offer a universal solution akin to transgene-mediated overexpression while avoiding the drawbacks associated with ***,we introduce an“in locus activation”technique in rice that leverages well-characterized STEs for refined,heritable,and multiplexed gene *** address the scarcity of potent enhancers,we developed a large-scale mining approach and discovered a suite of STEs that are capable of enhancing gene expression in rice *** in locus integration of these STEs into eight rice genes resulted in substantial tran-scriptional upregulation in the edited plants,with up to 869.1-fold increases in their transcript ***-ploying a variety of STEs,we achieved delicate control of gene expression,enabling the fine-tuning of key phenotypic traits such as plant *** approach also enabled efficient multiplexed gene upregu-lation,with up to four genes activated simultaneously,significantly enhancing the nicotinamide mononucleotide metabolic ***,heritability studies from the To to T3 generations confirmed the stable and heritable nature of STE-driven gene ***,our work demon-strates that coupled with STE mining,leveraging genome editing for in locus activation and gene upregu-lation holds great promise to be widely adopted in fundamental plant research and crop breeding.

关键词： genome editing short transcriptional enhancers gene upregulation crop breeding

Liver transplantation following two conversions in a patient with huge hepatocellular carcinoma and portal vein invasion:A case report

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World Journal of Gastroenterology 2024年第36期30卷 4071-4077页

作者： Li-Cong Liang Wen-Sou Huang Zhao-Xiong Guo Hong-Ji You Yong-Jian Guo Ming-Yue Cai Li-Teng Lin Guo-Ying Wang kang-Shun zhu Department of Minimally Invasive Interventional Radiology The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260Guangdong ProvinceChina Department of Nuclear Medicine The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260Guangdong ProvinceChina Department of Hepatobiliary Surgery The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510120Guangdong ProvinceChina

BACKGROUND Surgical resection and liver transplantation(LT)are the most effective curative options for hepatocellular carcinoma(HCC).However,few patients with huge HCC(>10 cm in diameter),especially those with portal vein tumor thrombus(PVTT),can receive these *** internal radiation therapy(SIRT)can be used as a conversion therapy for them because it has the dual benefit of shrinking tumors and increasing residual hepatic ***,in patients with huge HCC,high lung absorbed dose often prevents them from receiving *** SUMMARY A 35-year-old man was admitted because of emaciation and pain in the hepatic region for about 1 *** computed tomography scan showed a 20.2 cm×19.8 cm tumor located in the right lobe–left medial lobes with right portal vein and right hepatic vein *** the pathological type of HCC was confirmed by biopsy,two conversions were *** first one was drug-eluting bead transarterial chemoembolization plus hepatic arterial infusion chemotherapy and lenvatinib and sintilimab,converted to SIRT,and the second one was sequential SIRT with continued systemic *** tumor size significantly decreased from 20.2 cm×19.8 cm to 16.2 cm×13.8 cm,then sequentially to 7.8 cm×6.8 *** the meantime,the ratio of spared volume to total liver volume increased gradually from 34.4%to 55.7%,then to 62.9%.Furthermore,there was visualization of the portal vein,indicating regression of the tumor ***,owing to the new tumor in the left lateral lobe,the patient underwent LT instead of resection without major *** Patients with inoperable huge HCC with PVTT could be converted to SIRT first and accept surgery sequentially.

关键词： Hepatocellular carcinoma Two conversions Liver transplantation Yttrium-90 resin microspheres Transarterial chemoembolization Hepatic arterial infusion chemotherapy Case report

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高原丝路上建立吐蕃佛幢的汉藏因缘

世界宗教文化 2024年第4期 24-32页

作者：尹邦志康珠西南民族大学哲学学院

经过多年的研究,“高原丝绸之路”的历史存在已经取得了国内外学术界的共识,有关它的真相和更多的意义还有待更加广泛和深入的探索。从丝绸之路对佛教在亚洲的传播历史来看,高原丝绸之路为研究吐蕃佛教和汉藏佛教关系提供了新的视角,即... 详细信息

经过多年的研究,“高原丝绸之路”的历史存在已经取得了国内外学术界的共识,有关它的真相和更多的意义还有待更加广泛和深入的探索。从丝绸之路对佛教在亚洲的传播历史来看,高原丝绸之路为研究吐蕃佛教和汉藏佛教关系提供了新的视角,即从政教关系、教派源流和宗教领袖之外的立场,在一个更为宏阔的时间和空间维度上来看待佛教的历史和教义。本文聚焦于吐蕃时期桑耶寺建立佛法僧三宝齐备的法幢这一历史事件,立足于青藏高原丝绸之路来分析各方面的因缘,希望阐明汉藏佛教交流在特定的时空条件下具有其客观性和必然性,藏传佛教是文明交汇的成果。

关键词：高原丝绸之路吐蕃佛教汉藏交流中印交流佛教地缘关系

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数字峰会的溢出效应:基于股票流动性的研究

华东经济管理 2024年第1期38卷 79-88页

作者：朱康唐勇福州大学经济与管理学院福建福州350108

数字中国建设峰会是否会对资本市场形成冲击,尚缺乏可靠的经验证据。文章采用2010—2021年我国A股上市公司参展数字峰会的数据,结合渐进双重差分模型,分析企业参展数字峰会能否改善资本市场的股票流动性。研究发现:数字峰会提高了股票... 详细信息

数字中国建设峰会是否会对资本市场形成冲击,尚缺乏可靠的经验证据。文章采用2010—2021年我国A股上市公司参展数字峰会的数据,结合渐进双重差分模型,分析企业参展数字峰会能否改善资本市场的股票流动性。研究发现:数字峰会提高了股票流动性,在进行内生性及稳健性检验后结论仍然成立;机制检验表明,提高股票交易强度、提升分析师关注程度以及增强股吧活跃度是数字峰会产生溢出效应的主要途径;经进一步的异质性分析发现,数字峰会的溢出效应在信息环境较差的企业中更为显著,而且企业多次参展数字峰会更能促进股票流动性;在经济后果方面,发现数字峰会提高股票流动性具有缓解企业融资约束的作用。

关键词：数字峰会股票流动性溢出效应

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论中日茶文化中的横柄壶

农业考古 2024年第5期 93-102页

作者：詹嘉朱康景德镇陶瓷大学考古文博学院江西省文化和旅游厅陶瓷文明史研究中心景德镇陶瓷职业技术学院

横柄壶是中国传统器皿,唐代已引入茶事,功能随历代饮茶方式之不同而多有变化。18世纪中期,横柄壶传入日本,经煮茶器、煮水器、瀹茶具等数次功能转变,成为日本常见的茶壶样式。本文梳理了横柄壶在中日两国的发展历程,并结合文化审美、造... 详细信息

横柄壶是中国传统器皿,唐代已引入茶事,功能随历代饮茶方式之不同而多有变化。18世纪中期,横柄壶传入日本,经煮茶器、煮水器、瀹茶具等数次功能转变,成为日本常见的茶壶样式。本文梳理了横柄壶在中日两国的发展历程,并结合文化审美、造型功能等探析其在日本流行的原因。

关键词：横柄壶煎茶文化中日茶文化交流